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All Journal Pendidikan Biologi Jurnal Produksi Tanaman Atrium Pendidikan Biologi Serambi Biologi Sainstek : Jurnal Sains dan Teknologi TROPICAL GENETICS International Journal on Health and Medical Sciences Tropical Genetics Berita Biologi
Des M
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Veterinary Other

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Journal : TROPICAL GENETICS

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Design of primer Ipomoea batatas chloroplast gene matK S. Syamsurizal; Ardi Ardi; Des M; Resti Fevria; Yusni Atifah; Elsa Badriyya; Afifatul Achyar
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Sweet potato varieties (Ipomoea batatas) Pucuk Hitam Panyalaian and Madu Pucuk Hitam Panyalaian have the potential to be a superior commodity in West Sumatra, but the information of genetic diversity is very limited. Data on the diversity of sweet potato germplasm is beneficial in the selection of plants to obtain superior cultivars in plant breeding. Aim: The aim of the study was to find specific primers for the study of sweet potato genetic markers using Ipomoea batatas chloroplast genes matK. Methods: DNA extraction from sweet potato young leaves; designing forward and reverse primers Ipomoea batatas chloroplast genes matK; PCR; sequencing; bioinformatics analysis and species identification by comparing the NCBI database. Results: Plant DNA barcoding PCR using designed primers matK were successfully resulting single DNA band in different amplicon size in some samples. This indicating that the designed primers used were able to distinguish variation in one species.
Co-Authors Achyar, Afifatul Albar, Rahmat Anggaraini, Mita Annisa Tulhasanah Ardi Ardi Atifah, Yusni Berlinda Paradisa, Yashanti Chatri, Moralita Chatri Dwi Hilda Putri Elsa Badriyya Elsa Yuniarti Fevria, Resti Ficil Mikaf Mades Fifendy Moralita Chatri Novitasari, Yuliana Diyah Oki Jumaidi Rahmadhani Fitri Ratna Sari Aprilia Renta Dwi Ananda Rizka Meisy Evis Putri Rizki Nanda Putra Withman Rizki Rizki S. Syamsurizal Sari Ramadhani SOLICHATUN SOLICHATUN Violita Violita wahyuni wahyuni Zulyusri Zulyusri