Amiruddin Amiruddin
Laboratorium Klinik Fakultas Kedokteran Hewan Universitas Syiah Kuala

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Profil Hematologi Kelinci Setelah Implan Plate Material Logam (Profile Hematology Of Rabbit After Metal Plate Implant) Yolanda Sari; erwin erwin; Nuzul Asmilia; Zuhrawaty Zuhrawaty; Amiruddin Amiruddin; Muhammad Hambal
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 1 (2021): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i1.11797

Abstract

ABSTRAKPenelitian ini bertujuan mengamati jumlah eritrosit, hemoglobin, hematokrit dan trombosit kelinci setelah implan plate material logam. Penelitian ini menggunakan 6 ekor kelinci yang dibagi menjadi 2 kelompok. Kelompok 1 diimplan plate tantalum dan kelompok 2 diimplan plate besi. Pengambilan darah melalui vena auricularis pada hari ke 0, 7, 14, 28, dan 56. Setelah perlakuan jumlah eritrosit, hemoglobin, hematokrit dan trombosit dihitung menggunakan hematology analyzer. Hasil penelitian menunjukkan penurunan jumlah eritrosit, hemoglobin dan hematokrit setelah implan material logam dengan perbedaan tidak signifikan antar waktu pengamatan sedangkan jumlah trombosit menunjukkan perbedaan signifikan antar waktu pengamatan. Implan plate tantalum dan plate besi menyebabkan perubahan jumlah eritrosit, hemoglobin, hematokrit dan trombosit pada beberapa hari pengamatan. Kata kunci : eritrosit, hemoglobin, hematokrit, plate besi, plate tantalumABSTRACT            This study was aimed to determine the number of erythrocytes, hemoglobin, hematocrit and thrombocytes on rabbits after metal plate implant. This study used 6 rabbits that divided into 2 groups. Group 1 was implanted of tantalum plate and group 2 was implanted of iron plate. Taking blood through auricular vein on days 0, 7, 14, 28, dan 56. After treatment the number of erythrocytes, hemoglobin, hematocrit and thrombocytes were calculated with the hematology analyzer. The results showed a decrease in the number of  erythrocytes, hemoglobin and hematocrit after metal plate implant with no significant difference between the time of observation while trombocytes showed a significant difference between the time of observation. Tantalum implant and iron plate causes changes in the number of erythrocyte, hemoglobin, hematocrit and thrombocyte on several days of observation. Keywords: total of erythrocyte, hemoglobin, hematocrit, thrombocyte, iron plate, tantalum plate
Sensitivity Test of Bandotan Leaf Extract (Ageratum conyzoides) Against Pseudomonas aeruginosa Bacteria Masda Admi; Yuni Sari; Rasmaidar Rasmaidar; Amiruddin Amiruddin; T Zahrial Helmi; Yusrizal Akmal; M Isa
The International Journal of Tropical Veterinary and Biomedical Research Vol 7, No 1 (2022): Vol. 7 (1) May 2022
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.256 KB) | DOI: 10.21157/ijtvbr.v7i1.28504

Abstract

  The leaves of Bandotan (Ageratum conyzoides) are a plant thought to have antibacterial properties. This study aims to determine the sensitivity of Bandotan leaf extract in inhibiting the growth of the bacteria Pseudomonas aeruginosa. This study used a stock extract of Bandotan leaves from the Pharmacology Laboratory and a bacterial isolate of P. aeruginosa in the Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala, which was identified by Gram staining, indole test, Methyl Red test, and confectionery test. The research method was carried out by planting the re-identified bacterial isolates on Nutrient Broth (NB) media, incubated at 37ºC for 24 hours. Then the turbidity composition of the isolates was arranged to match the turbidity in 0.5 McFarland solution. Furthermore, the sensitivity test of the extract on Mueller Hinton Agar (MHA) media was carried out by levelling the bacterial isolates on the surface of the media and attaching a disc containing bandotan leaf extract with a concentration of 25%, 50%, 75% and gentamicin disk as a positive control and distilled water as a negative control. All treatments were incubated at 37ºC for 24 hours, and then the inhibition zone was measured using millimeters (mm) callipers. The results showed that concentrations of 25%, 50% and 75%, respectively, had an inhibition zone of 8.16 mm, 9.82 mm, and 16.08 mm, respectively. In contrast, the average inhibition zone for gentamicin was 25, 30 mm and 0 mm distilled water. Therefore, it can be concluded that the Bandotan leaf extract is sensitive to growth inhibition of P. aeruginosa bacteria.
Sensitivity Test of Bandotan Leaf Extract (Ageratum conyzoides) Against Pseudomonas aeruginosa Bacteria Masda Admi; Yuni Sari; Rasmaidar Rasmaidar; Amiruddin Amiruddin; T Zahrial Helmi; Yusrizal Akmal; M Isa
The International Journal of Tropical Veterinary and Biomedical Research Vol 7, No 1 (2022): Vol. 7 (1) May 2022
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v7i1.28504

Abstract

  The leaves of Bandotan (Ageratum conyzoides) are a plant thought to have antibacterial properties. This study aims to determine the sensitivity of Bandotan leaf extract in inhibiting the growth of the bacteria Pseudomonas aeruginosa. This study used a stock extract of Bandotan leaves from the Pharmacology Laboratory and a bacterial isolate of P. aeruginosa in the Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala, which was identified by Gram staining, indole test, Methyl Red test, and confectionery test. The research method was carried out by planting the re-identified bacterial isolates on Nutrient Broth (NB) media, incubated at 37ºC for 24 hours. Then the turbidity composition of the isolates was arranged to match the turbidity in 0.5 McFarland solution. Furthermore, the sensitivity test of the extract on Mueller Hinton Agar (MHA) media was carried out by levelling the bacterial isolates on the surface of the media and attaching a disc containing bandotan leaf extract with a concentration of 25%, 50%, 75% and gentamicin disk as a positive control and distilled water as a negative control. All treatments were incubated at 37ºC for 24 hours, and then the inhibition zone was measured using millimeters (mm) callipers. The results showed that concentrations of 25%, 50% and 75%, respectively, had an inhibition zone of 8.16 mm, 9.82 mm, and 16.08 mm, respectively. In contrast, the average inhibition zone for gentamicin was 25, 30 mm and 0 mm distilled water. Therefore, it can be concluded that the Bandotan leaf extract is sensitive to growth inhibition of P. aeruginosa bacteria.