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All Journal Jurnal Sain Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter JURNAL PETERNAKAN Jurnal Peternakan Indonesia Vitek : Bidang Kedokteran Hewan J-Kesmas: Jurnal Kesehatan Masyarakat (Indonesian Journal of Public Health) Arwana: Jurnal Ilmiah Program Studi Perairan The International Journal of Tropical Veterinary and Biomedical Research Buletin Pengabdian Jurnal Medika Veterinaria
Masda Admi
Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh Dewi, Maryulia; Darmawi, Darmawi; Nurliana, Nurliana; Karmil, T. Fadrial; Helmi, T. Zahrial; Fakhrurrazi, Fakhrurrazi; Erina, Erina; Abrar, Mahdi; AK, M. Daud; Admi, Masda
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.862 KB) | DOI: 10.22146/jsv.57359

Abstract

Biofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium was used to biofilm bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were λ 595 nm. Data were analyzed by using Analysis of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive.
Mucosal Mast Cells Contribution in Intestinal Defense of Chickens (Gallus domesticus) Infected Naturally by Ascaridia galli Ummu Balqis; Masda Admi; Darmawi Darmawi; Muhammad Hambal
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.91 KB)

Abstract

This study was aimed at finding out the investigation of mucosal mast cells   in intestines of chicken  that  were  naturally  infected  by  Ascaridia galli.  Amount  of  ten  intestine  of  freshly slaughtered chickens (Gallus domesticus) found from local abatoir in Banda Aceh were divided into two groups containing five intestines of each. Mucosal mast cells count were done of which histologic slides were made in stained serial histological sections with Alcian blue (pH 0,3) and Safranin-O (pH 0,1) of the intestines. The result showed that the mucosal mast cells increased significantly (P 0.05) in intestines of chickens infected naturally by survival A. galli adult worms. It was concluded that the intestinal defense of chickens against parasite infection is associated with the mucosal mast cells contribution by creating an environment hostile to the establishment and survival of intestinal nematodes, A. galli.
Inhibition of Salmonella sp. Growth in Comparison with Antibiotics and Pineapple (Ananas comosus) Squeeze Masda Admi; Erina Erina; Azhari Azhari; Ummu Balqis; Muhammad Hambal; Darmawi Darmawi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1063.056 KB)

Abstract

The purpouse of this study to examine antibiotics inhibition growth of Salmonella sp. isolated from feces of broiler chickens to chloramphenicol, gentamicin, and tetracycline in coparison with pineapple squeeze. Swab  sample  from  broiler  chicken  cloaca  was  cultured  to  nutrient  broth  media  and incubated at 37°C temperature for 24 hours. Culture was compared with Mc Farland 3 standardization, and separated on surface of Muller Hinton Agar (MHA). Antibiotic sensitivity was tested by mean of  chloramphenicol, gentamisin, and tetrasiklin in coparison with pineapple squeeze. The antibiotic disks (Oxoid) were put on the surface of MHA media which had inoculated with Salmonella sp. bacteria. Antibiotic sensitivity was determined based on formation of inhibition zone in surrounding of antibiotic disc. The result showed that Salmonella sp. bacteria growth was inhibited in ≥ 21 mm and ≥ 18 mm, ≥ 7 mm and ≥ 9 mm against chloramphenicol, gentamisin, tetrasiklin, and pineapple squeeze respectively. This research concluded that the Salmonella sp. cultured in this manner were sensitive to chloramphenicol and gentamisin. The inhibition growth of Salmonella sp. caused by chloramphenicol and gentamicin was significantly higher in comparison with pineapple (Ananas comosus) squeeze, meanwhile the Salmonella sp. bacteria was resistance against tetracycline. Our result suggest that both antibiotics chloramphenicol and gentamicin were recommended for antibiotic therapy in Salmonella sp. infection.
Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh (Antibiotic Activities To Staphylococcus aureus Biofilms Of Aceh Cattle Preputium Isolate) Maryulia Dewi; Darmawi Darmawi; Nurliana Nurliana; T. Fadrial Karmil; T Zahrial Helmi; Fakhrurrazi Fakhrurrazi; Erina Erina; Mahdi Abrar; M. Daud AK; Masda Admi
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.35219

Abstract

AbstractBiofilm  is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB)  medium were used to biofilm. Bacterial  while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were  λ 595 nm. Data were analyzed by using Analysis Of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive. Keywords: biofilm, antibiotics, Staphylococcus aureus, cows aceh.                Abstrak Biofilm merupakan salah satu parameter untuk menentukan jalur patogenisitas suatu bakteri. Antibiotik dapat melemahkan aktivitas virulensi bakteri dengan menghambat pembentukan biofilm. Penelitian ini bertujuan untuk melihat karaksteristik dan aktivitas antibiotik terhadap biofilm Stapylococcus aureus isolat preputium sapi Aceh. Penelitian bersifat eksplorasi dan eksperimental, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan menggunakan tiga jenis antibiotik, tiga konsentrasi dan tiga kali ulangan. Antibiotik yang digunakan adalah tetrasiklin, oksitetrasiklin dan fosfomisin dengan konsentrasi masing-masing 10%, 20% dan 30% (mg/mL). Pengujian biofilm dilakukan pada media Nutrient Broth (NB), menggunakan mikroplat U-bottom polystyrene 96 wells. Pewarnaan biofilm menggunakan kristal violet 1%. Optical Density (OD) biofilm dibaca pada    λ 595 nm. Data hasil penelitian dianalisis dengan menggunakan Analysis Of Variance (ANOVA). Hasil penelitian menunjukkan aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm tergolong positif moderat, yaitu 2,667cell/µL. Uji Biofilm menunjukkan jenis dan konsentrasi antibiotik berpengaruh secara nyata (P<0,05) terhadap daya hambat biofilm Staphylococcus aureus isolat preputium sapi Aceh. Tetrasiklin 30% (mg/mL) mempunyai kemampuan paling baik menghambat pembentukan biofilm Staphylococcus aureus isolat preputium sapi Aceh sebesar 52,18% menjadi 1,262 cell/µL, sehingga aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm dari positif moderat menjadi positif palsu. Kata Kunci: biofilm, antibiotik, Staphylococcus aureus, sapi aceh.
Efektifitas ekstrak daun nenas (Ananas cosmosus) terhadap penetasan telur ikan bandeng (Chanos chanos Forskall) Muhammad Walidin; Masda Admi
Arwana: Jurnal Ilmiah Program Studi Perairan Vol 2 No 2: November 2020
Publisher : Program Studi Akuakultur, Fakultas Pertanian, Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/jipsbp.v2i2.387

Abstract

Pemijahan mengasilkan telur-telur ikan yang kemudian ditetaskan untuk menghasilkan benih. Jamur sering dijumpai menyerang telur ikan, jamur akan membalut telur ikan dengan ciri-ciri seperti benang dan telur tidak akan menetas dan kemudian membusuk. Daun nenas memiliki senyawa yang sama dengan kulit buah nenas yaitu flavonoid, folifenol, alkaloid dan fenol yang berfungsi sebagai antibakteri dan antimikroba tetapi dengan jumlah berbeda. Penggunaan ekstrak daun nenas dapat mencegah dan membunuh mikroba yang menyerang telur ikan. Penelitian telah dilakukan pada bulan Januari 2016 dan Bulan Februari 2017 menggunakan metode eksperimental sebanyak 3 perlakuaan dosis ekstraksi daun nenas dengan masing-masing 3 ulangan. Perlakuan dosis yaang diberikan adalah 0 ml/ 5 liter air, 20 ml/ 5 liter air dan 30 ml/ 5 liter air. Hasil penelitian menunjukkan penggunaan ekstrak daun Nenas dengan konsentrasi 30 ml/ 5 liter meningkatkan persentase daya tetas sebesar 77%, menurunkan tingkat abnormalitas menjadi 8,95% dan meningkatkan tingkat kelangsungan hidup larva ikan bandeng sebesar 87,87%. Hasil terbaik terdapat pda perlakuan 30 ml/ 5 liter air.
Isolasi dan Identifikasi Bakteri Gram Positif Staphylococcus aureus dan Micrococcus pada Peternakan Sapi yang Terindikasi Mastitis Siti Rani Ayuti; Widya Nur Hidayati; Masda Admi; Rosmaidar Rosmaidar; Zainuddin Zainuddin; Hennivanda Hennivanda; Ali Makmur
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 25, No 1 (2023): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.25.1.98-109.2023

Abstract

Mastitis menjadi salah satu penyakit yang menyebabkan penurunan produksi ternak dan sulit disembuhkan dengan menggunakan antibiotik dan dapat menimbulkan residu, serta memicu resistensi antibiotik. Penelitian ini bertujuan untuk mengetahui bakteri Gram positif yang dapat menjadi penyebab mastitis pada sapi. Penelitian menggunakan sampel yang diambil dari peternakan rakyat dengan cara swab ambing ternak. Isolasi bakteri dilakukan dengan cara penanaman pada media Mannitol Salt Agar (MSA) dan melakukan Identifikasi dengan pewarnaan Gram, Uji Katalase dan Uji biokimia (maltosa dan laktosa). Data dianalisis menggunakan analisis deskriptif yaitu jenis-jenis bakteri sebagai agen penyebab mastitis. Hasil penelitian yang didapatkan sebagai bakteri penyebab mastitis spesies Staphylococcus aureus dan Micrococcus sp. yang tergolong pada bakteri Gram positif. Dapat disimpulkan bahwa pada sapi terindikasi mastitis terdapat bakteri Gram positif, hal tersebut secara morfologi diidentifikasi yaitu Staphylococcus aureus dan Micrococcus.
Isolation and Identification of Gram-Negative Bacteria on Cattle Farms Indicated by Mastitis Siti Rani Ayuti; Zahwa Amelia Pratiwi; Masda Admi; Darniati Darniati; M Isa; Herrialfian Herrialfian
The International Journal of Tropical Veterinary and Biomedical Research Vol 7, No 2 (2022): Vol. 7 (2) November 2022
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1636.968 KB) | DOI: 10.21157/ijtvbr.v7i2.30735

Abstract

Mastitis is one  of the causes  of diseases that can reduce livestock production.  Mastitis can be caused by various types of bacteria that cause a decrease in livestock production that is difficult to cure with antibiotics has been reported resistance. This study aims to determine gram-negative bacteria that can be the cause of mastitis in cows. The study used samples taken from folk farms by means of livestock udder swabs. Bacterial isolation is carried out by  planting in Mannitol Salt Agar (MSA) media  and identifying by Gram staining  and  biochemical tests (maltose and lactose). The data analysis used is descriptive analysis, namely the types of bacteria as the causative agents of mastitis.  The results of the study obtained bacteria that cause mastitis of  gram-negative species Klebsiella  sp and Enterobacter sp yang classified as Gram-negative  bacteria. It can be concluded that in cows indicated mastitis there are Gram-negative bacteria, these are morphologically identified, namely Klebsiella  sp and Enterobacter sp.
Sensitivity Test of Bandotan Leaf Extract (Ageratum conyzoides) Against Pseudomonas aeruginosa Bacteria Masda Admi; Yuni Sari; Rasmaidar Rasmaidar; Amiruddin Amiruddin; T Zahrial Helmi; Yusrizal Akmal; M Isa
The International Journal of Tropical Veterinary and Biomedical Research Vol 7, No 1 (2022): Vol. 7 (1) May 2022
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v7i1.28504

Abstract

  The leaves of Bandotan (Ageratum conyzoides) are a plant thought to have antibacterial properties. This study aims to determine the sensitivity of Bandotan leaf extract in inhibiting the growth of the bacteria Pseudomonas aeruginosa. This study used a stock extract of Bandotan leaves from the Pharmacology Laboratory and a bacterial isolate of P. aeruginosa in the Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala, which was identified by Gram staining, indole test, Methyl Red test, and confectionery test. The research method was carried out by planting the re-identified bacterial isolates on Nutrient Broth (NB) media, incubated at 37ºC for 24 hours. Then the turbidity composition of the isolates was arranged to match the turbidity in 0.5 McFarland solution. Furthermore, the sensitivity test of the extract on Mueller Hinton Agar (MHA) media was carried out by levelling the bacterial isolates on the surface of the media and attaching a disc containing bandotan leaf extract with a concentration of 25%, 50%, 75% and gentamicin disk as a positive control and distilled water as a negative control. All treatments were incubated at 37ºC for 24 hours, and then the inhibition zone was measured using millimeters (mm) callipers. The results showed that concentrations of 25%, 50% and 75%, respectively, had an inhibition zone of 8.16 mm, 9.82 mm, and 16.08 mm, respectively. In contrast, the average inhibition zone for gentamicin was 25, 30 mm and 0 mm distilled water. Therefore, it can be concluded that the Bandotan leaf extract is sensitive to growth inhibition of P. aeruginosa bacteria.
1. The Sensitivity Level Of Gentamicine, Cholramphenicol and Penicillin Inhibiting The Growth Of Pseudomonas Aeruginosa Bacteria Isolate From Aceh Bull Prepunce Mada Admi; Annisa Anwar Sitorus; Rinidar Rinidar; Amalia Sutriana; Rosmaidar Rosmaidar; Sugito Sugito
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.20856

Abstract

This study aims to determine the sensitivity level of gentamicin, chloramphenicol and penicillin antibiotics to inhibit the bacterial growth of P. aeruginosa taken from preputium isolate of Aceh cattle. This Stock of Pseudomonas aeruginosa isolate from Microbiology Laboratory of FKH USK, re-identified its purity through: indole test, methyl red test, sugar test and as well as on Nutrient Broth (NB) media; incubated at 37ºC for 24 hours; observed the turbidity level of 0.5 Mc Farland solution. Then, the antibiotic sensitivity test was carried out using the method of Mueller Hinton Agar (MHA). The observations were made by measure the inhibition zone using the calipers in millimeters (mm) against antibiotics. The results of the observation of the inhibition zone on the gentamicin antibiotic was averaged of 25.5 mm, 23.7 mm chloramphenicol, and 12.1 mm penicillin. This study concluded that the gentamicin, chloramphenicol, and penicillin antibiotic were still effective against P. aeruginosa bacteria with the highest level of sensitivity seen in gentamicin antibiotic. 
Assistance in agricultural waste fermentation technology in Krueng Kalee Village, Darussalam Sub-District, Aceh Besar [Pendampingan teknologi fermentasi limbah pertanian di Gampong Krueng Kalee Kecamatan Darussalam Aceh Besar] Admi, Masda; Azhari, A; Syah, Hendri
Buletin Pengabdian Vol 4, No 3 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v4i4.42666

Abstract

Agricultural waste holds significant potential as a source of animal feed and liquid organic fertilizer through the application of fermentation technology. The types of agricultural waste that can be utilized include water spinach leaves, sweet potato leaves, and spinach leaves. The utilization of these wastes not only adds value but also contributes to enhancing the economic independence of communities. Using waste as animal feed offers a sustainable alternative, especially during dry seasons and planting periods, with the potential for higher nutritional content following the fermentation process. The aim of this community service initiative is to educate the public about the types of agricultural and plantation waste that can be used as animal feed, as well as the risks to be avoided related to the effects of fermented feed. Additionally, the program involves training the community in the application of fermentation technology on rice straw, vegetable waste, and cassava leaves, utilizing microbial starters derived from the rumen of goats and cows that have adapted to cyanide toxins. The methods employed in this initiative include outreach and hands-on practice in the production of fermented feed and liquid organic fertilizer. The results indicate a high level of enthusiasm among community members regarding the dissemination of information on utilizing agricultural waste as materials for fermented feed and liquid organic fertilizer, as well as an increased awareness of the importance of environmental preservation surrounding residential and agricultural areas. In conclusion, this initiative has fostered a commitment from partners to independently implement fermentation processes, along with a hope for the continuation of further community service programs focused on the application of technology to enhance the economic conditions of farmers and livestock farmer.
Co-Authors - Fakhrurrazi . Darniati . Nurliana Abd. Rasyid Syamsuri Abdul Harris Abdullah Hamzah, Abdullah Ali Makmur Amalia Sutriana Amiruddin Amiruddin Annisa Anwar Sitorus Ayuti, Siti Rani Azhari Azhari Azhari Azhari Azhari, A Daniel, Daniel Daniel Darmawi Darmawi Darmawi Darmawi Darniati Darniati Dasrul Dasrul Erina Erina Erina Erina Erina Erina Faisal Jamin Faisal Mustafa Fajar Fakri Fajri, Fauzan Fakhrurrazi Fakhrurrazi Fitri, Khairani Gholib Gholib, Gholib Ginta Riady Helmi, T. Zahrial Hendri Syah Hennivanda Hennivanda Hennivanda, Hennivanda Herrialfian Herrialfian Herrialfian, Herrialfian Isa, M Kurniawan, Wilda Lestari, Rafika M Daud AK M Isa M Isa M.iSA - Mahdi Abrar Maryulia Dewi Maryulia Dewi Maulida, Rafdhayatul Muhammad Hambal Muhammad Walidin Mulyadi Adam, Mulyadi Muslim Akmal Nanda Yulian Syah Nora Usrina NURLIANA NURLIANA Nurzalika, Siti Pratiwi, Zahwa Amelia putri, anggun bellia Rasmaidar Rasmaidar Rastina, Rastina Rinidar Rinidar Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Siti Rani Ayuti Siti Rani Ayuti Sugito Sugito Syafruddin Syafruddin T Zahrial Helmi T Zahrial Helmi T. Fadrial Karmil T. Fadrial Karmil Teuku Zahrial Helmi Triva Murtina Lubis Ummu Balqis Wardhani, Lailia Dwi Kusuma Widya Nur Hidayati Winaruddin Winaruddin Yuni Sari Yusrizal Akmal Zahwa Amelia Pratiwi Zainuddin Zainuddin Zainuddin, Zainuddin Zulfitri Zulfitri, Zulfitri Zulkifli, Baidillah