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KAJIAN ETHYLENE TRIPLE RESPONSE TERHADAP KECAMBAH TIGA VARIETAS KACANG HIJAU Kristiani Walesasi; Feky R Mantiri; Herny Simbala; Marhaenus Rumondor
JURNAL ILMIAH SAINS Volume 16 Nomor 2, Oktober 2016
Publisher : Sam Ratulangi University

KAJIAN ETHYLENE TRIPLE RESPONSE TERHADAP KECAMBAH TIGA VARIETAS KACANG HIJAU ABSTRAK Pembudidayaan tanaman kacang hijau selalu terkendala dengan keadaan lahan yang terbatas. Tanaman kacang hijau di tanam dengan tumpang sari, sehingga mengakibatkan tanaman ini tumbuh dengan keadaan ternaungi. Naungan ini menyebabkan naiknya produksi etilen, sehingga menimbulkan triple response yang dapat mempengaruhi pertumbuhan dan perkembangan tanaman. Oleh karena itu hal ini menjadi alasan dilakukan penelitian ini dengan etilen pada tiga varietas kacang hijau yaitu VIMA I, Seriti dan Kutilang. Setelah dikecambahkan tujuan untuk menyeleksi varietas kacang hijau yang tahan dengan adanya peningkatan jumlah selama 5 hari, panjang kacang hijau varietas VIMA I yang diberi karbid (penghasil etilen) adalah yang tertinggi. Bengkokan varietas kacang hijau yang terendah adalah varietas VIMA I. Namun untuk diameter kecambah ketiga varietas tidak berbeda nilainya baik yang diberi karbid maupun yang tidak diberi karbid. Dari penelitian ini menunjukkan varietas VIMA I adalah tanaman yang tahan terhadap peningkatan etilen, sehingga varietas ini berpotensi untuk ditanam di naungan. Kata Kunci :Etilen, Triple Response, Kacang Hijau STUDY OF ETHYLENE TRIPLE RESPONSE ON SEEDLINGS OF THREE VARIETIES OF MUNGBEAN ABSTRACT Grow green beans are always constrained by the state of limited land. Thus the green bean crop planted with intercropping, resulting in these plants grow with shady circumstances. However, this shade causes increased production of ethylene, causing triple response which can affect plant growth and development. Therefore this is the reason of this research with the aim of selecting resistant varieties of green beans with the increased amount of ethylene on three varieties of green beans that VIMA I, Seriti and Kutilang. Once germinated for 5 days, the length of green beans varieties by VIMA I carbide (ethylene producer) is the highest. And bend varieties of green beans were the lowest observed varieties VIMA is I who have given carbide. But for the three varieties of sprouts diameter does not vary in value whether it is given or not given acetylene carbide. From this research shows VIMA I is a plant varieties that are resistant to an increase in ethylene, so that this variety has the potential to be planted in the shade. Keywords: Ethylene, Triple Response, Mung Bean

EVALUASI TAHAN BANJIR PADI LOKAL SULAWESI UTARA PADA FASE VEGETATIF DENGAN VARIASI WAKTU PERENDAMAN Risky Hiskia Poluan; Ai S. Nio; Feky Recky Mantiri
JURNAL ILMIAH SAINS Volume 17 Nomor 1, April 2017
Publisher : Sam Ratulangi University

ABSTRAK Banjir merupakan salah satu kendala yang menghambat produktivitas padi. Masalah ini dapat diselesaikan dengan beberapa cara, salah satunya dengan strategi adaptasi. Oleh karena itu perlu dilakukan kajian tentang adaptasi banjir khusunya pada padi lokal Sulawesi Utara (Superwin, Burungan, Temo dan Ombong). Penelitian telah dilaksanakan untuk mengakaji pengaruh cekaman banjir terhadap padi lokal Sulawesi Utara pada fase vegetatif. Penelitian ini dilakukan dengan 4 varietas padi lokal Sulawesi Utara (Superwin,Â Ombong, Temo dan Burungan) dengan 12 kali ulangan dan perlakuan dilakukan pada saat padi berusia 2 minggu. Perlakuannya yaitu dengan membanjiri tanaman padi yang berusia 2 minggu hingga hari ke-20. Dari hasil penenlitian ini tinggi tanaman dari keempat varietas padi lokal Sulawesi Utara sama-sama menunjukkan pertumbuhan yang baik sehingga bisa dijadikan indikator padi yang tahan banjir. Berdasarkan jumlah daun dari keempat varietas varietas Superwin yang memiliki daun terbanyak dibanding dengan ketiga varietas lainnya, namun secara keseluruhan dari jumlah daun setiap varietas bisa bertahan dalam banjir sehingga bisa dipakai sebagai indikator padi yang tahan banjir. Kata kunci: tinggi tanaman, jumlah daun, padi lokal Sulawesi Utara, fase vegetatif, banjir Â EVALUATION OF THE LOCAL FLOOD-RESISTENT RICE NORTH SULAWESI THE VEGETATIVE PHASE WITH THE VARIATION OF IMMERSION TIME ABSTRACT Flood is one of the problems that hinder rice production. This problem can be solved in several way, such as adaptation strategies. It is therefore necessary to study about the adaptation of flooding especially to the local rice of North Sulawesi (Superwin, Burungan, Temo and Ombong). This research has been conducted to assess the effect of flooding stress to the local rice of North Sulawesi on the vegetative phase. This research was conducted by four local rice varieties (i.e., Superwin, Ombong, Temo and Burungan) with 12 repetitions and treatments carried out during 2 weeks old rice. The treatment is to flood the rice plants 2 weeks old up to the 20th day. From this research, plant height for each of four local rice varieties show good growth so it can be used as indicator of flood-resistant rice. Based on the number of leaves of four varieties, Superwin has most leaves compared with the three other varieties, but overall on the number of leaves each variety can survive in flood that could be used as an indicator of flood-resistant rice. Keywords: plant height, leaf numbers, local rice of North Sulawesi, vegetative phase, flood

KAJIAN VARIASI SEKUNES INTRASPESIES DAN FILOGENETIK MONYET HITAM SULAWESI (Macaca nigra) DENGAN MENGGUNAKAN GEN COI Fitri Elisabeth Br. Hasibuan; Feky R Mantiri; Rooije R.H Rumende
JURNAL ILMIAH SAINS Volume 17 Nomor 1, April 2017
Publisher : Sam Ratulangi University

KAJIAN VARIASI SEKUNES INTRASPESIES DAN FILOGENETIK MONYET HITAM SULAWESI (Macaca nigra) DENGAN MENGGUNAKAN GEN COIÂ ABSTRAKMacaca nigra merupakan salah satu spesies yang endemik dan terancam punah di Sulawesi Utara. Eksploitasi yang berlebihan serta alih fungsi hutan menjadi ancaman bagi spesies ini di alam. Penelitian ini dilakukan untuk mengetahui variasi sekuens intraspesies M. nigra yang berada di Pusat Penyelamatan Satwa Tasikoki Bitung. Analisis sekuens menunjukkan terdapat perbedaan sepuluh pasang basa nukleotida pada urutan sekuens sampel dilokasi yang berbeda. Jarak genetik antara kedua sampel yaitu 0.016. Hasil perhitungan jarak genetik menunjukkan variasi genetik masih berada dalam kisaran variasi intraspesies dengan ambang batas untuk variasi intraspesies yaitu 0.015-0.025. Selain itu, variasi juga ditunjukkan pada sampel dengan kerabat dekatnya yang terdata di GenBank disebabkan karena adanya mutasi sinonim dan mutasi nonsinonim. Analisis filogenetik berdasarkan gen COI (Cytochrome Oxidase-I) menunjukkan bahwa sampel M. nigra yang digunakan dalam penelitian ini berada satu klaster dengan M. nigra yang ada di database dan termasuk ke dalam kelompok Silenus.Kata kunci: Variasi sekuens intraspesies, Gen COI, Macaca nigra, analisis filogenetik.Â THE STUDY INTRASPECIFIC VARIATION SEQUENCES AND PHYLOGENETIC CELEBES BLACK MACAQUE (Macaca nigra) USING COI GENEÂ ABSTRACTMacaca nigra is listed as one of the endemic species and endangered in North Sulawesi. Exploitation and forest conversion have become threats to this species in the wild. This study was conducted to determine the intraspecific sequence variation of M. nigra located in Tasikoki Wildlife Rescue Center, Bitung. Sequence analysis revealed ten nucleotides differences between these two specimens. Genetic distance for both of specimens is 0.016. The result of genetic distance, the genetic variation between the specimens of M. nigra was still within the range of intraspecific variation. Distance analysis was also conducted by comparing with the close relatives of M. nigra based on BLAST search, which showed range from 0.015-0.025. These differences resulted in both synonymous and nonsynonymous mutation. Phylogenetic analysis using DNA sequences of COI (Cytochrome Oxidase-I) gene revealed that the two specimens of M. nigra claster together with M. nigra sequences that have been deposited in GeneBank. Moreover M. nigra is claster in the silenus group which is in accordance with previous reports.Keywords: Intraspecific Sequence Variation, COI Gene, Macaca nigra, Phylogenetic analysis.

Isolation and Characterization of Potential Lignocellulosic Degrading Bacteria From chicken Manure Compost Zhafira Tiza Maesharoh Soleman; Feky Mantiri; Marhaenus Rumondor
JURNAL BIOS LOGOS Vol. 13 No. 2 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i2.42791

Lignocellulose is the main component that can be found in plants. Lignocellulose consists of three components, namely hemicellulose, lignin, and cellulose. The maximum utilization of lignocellulose cannot be carried out without being degraded, however there are difficulties in carrying out the degradation. The difficulty faced in the degradation process is the presence of lignin components that provide strength and stiffness to the plant, which make it is quite resistant to the degradation process. Lignocellulose degradation requires delignification. The delignification process can be facilitated by enzymes produced by a number of microorganisms, including bacteria can produce lignocellulosic enzymes. This study aims to isolate and characterize lignocellulose-degrading bacteria in chicken manure, and to assess the ability of bacteria to degrade lignocellulose. The results obtained from this research showed that an isolate which has high similarity with Shigella flexneri bacteria which was found in chicken manure and exhibits a potential to degrade lignocellulose.

Isolation and Characterization of Potential Lignocellulosic Degrading Bacteria From chicken Manure Compost Zhafira Tiza Maesharoh Soleman; Feky Mantiri; Marhaenus Rumondor
JURNAL BIOS LOGOS Vol. 13 No. 2 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i2.42791

Lignocellulose is the main component that can be found in plants. Lignocellulose consists of three components, namely hemicellulose, lignin, and cellulose. The maximum utilization of lignocellulose cannot be carried out without being degraded, however there are difficulties in carrying out the degradation. The difficulty faced in the degradation process is the presence of lignin components that provide strength and stiffness to the plant, which make it is quite resistant to the degradation process. Lignocellulose degradation requires delignification. The delignification process can be facilitated by enzymes produced by a number of microorganisms, including bacteria can produce lignocellulosic enzymes. This study aims to isolate and characterize lignocellulose-degrading bacteria in chicken manure, and to assess the ability of bacteria to degrade lignocellulose. The results obtained from this research showed that an isolate which has high similarity with Shigella flexneri bacteria which was found in chicken manure and exhibits a potential to degrade lignocellulose.

Isolation and Identification of Thermophilic Bacteria from Lahendong Hot Spring, North Sulawesi Christiana Abrita Nathania Corneles; Feky Mantiri; Marina Singkoh
JURNAL BIOS LOGOS Vol. 13 No. 2 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i2.50184

Thermophilic bacteria are a group of procaryotic organisms that can grow in high temperature around 450C until 900C. Thermophilic bacteria are able to produce lipase thermostable enzyme. Lipase thermostable enzymes produced from thermophilic bacteria are able to catalyze the hydrolysis of triglycerides into glycerol and free fatty acids and the synthesis of esters in organic solvents. Lipase thermostable enzymes are potential to be used in industrial production. Lipase enzymes have been used for the production of laundry detergents as additives or to replace chemical detergents because of their environmental friendliness. This research was aimed to isolates and identified themophilic bacteria from Lahendong hot springs, North Sulawesi and to conduct the morphological characterization, biochemical test, and molecular identification with 16S rRNA. Results showed that isolates obtained from the- Lahendong hot spring have high similarities with Geobacillus kaustophilus, Bacillus cereus, and Geobacillus lituanicus. The three isolates are able to produce lipase thermostbale enzyme.

Isolation and Identification of Thermophilic Bacteria from Lahendong Hot Spring, North Sulawesi Christiana Abrita Nathania Corneles; Feky Mantiri; Marina Singkoh
JURNAL BIOS LOGOS Vol. 13 No. 2 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i2.50184

Thermophilic bacteria are a group of procaryotic organisms that can grow in high temperature around 450C until 900C. Thermophilic bacteria are able to produce lipase thermostable enzyme. Lipase thermostable enzymes produced from thermophilic bacteria are able to catalyze the hydrolysis of triglycerides into glycerol and free fatty acids and the synthesis of esters in organic solvents. Lipase thermostable enzymes are potential to be used in industrial production. Lipase enzymes have been used for the production of laundry detergents as additives or to replace chemical detergents because of their environmental friendliness. This research was aimed to isolates and identified themophilic bacteria from Lahendong hot springs, North Sulawesi and to conduct the morphological characterization, biochemical test, and molecular identification with 16S rRNA. Results showed that isolates obtained from the- Lahendong hot spring have high similarities with Geobacillus kaustophilus, Bacillus cereus, and Geobacillus lituanicus. The three isolates are able to produce lipase thermostbale enzyme.

Efektivitas Feromon terhadap Interest Kumbang Tanduk (Oryctes rhinoceros) pada Tanaman Kelapa (Cocos nucifera L.) Putri Sri Anggini; Lalu Wahyudi; Feky Recky Mantiri
JURNAL BIOS LOGOS Vol. 12 No. 1 (2022): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v12i1.40116

(Article History: Received Mar 1, 2022; Revised Mar 14, 2022; Accepted Apr 11, 2022)The population of Oryctes rhinoceros that out of control at coconut plantation could be effect on productivity. Therefore, necessary to control using pheromone traps. Pheromones as chemical compounds use to reproduction communicate between individual beetles. The purpose of this study was to reveal the potential of synthetic pheromones in order to control the population of horn beetle (O. rhinoceros). This study conducted observation and response of application pheromone to the population of O. rhinoceros and offcourse the leaf counting as parametre of the degree bettles offensive. The analyze quantitative of sample with associated the decrease coconut productivity. The results of this study indicate that pheromone traps was effective to control the O. rhinoceros by the number of beetles trapped at the area coconut plantations in Pineleng Village, there are160 tails and the ratio of male and female beetles was 1:2. Representative in this area was classified high degree offensive where obtained 6-7 beetles/ha and 45% losses of productivity.Keywords: Oryctes rhinoceros L.; pheromones; coconut plant; attack intensity.Abstrak Populasi kumbang Oryctes rhinoceros yang meningkat secara berkepanjangan tanpa adanya pengendalian dapat menyebabkan penurunan produktivitas kelapa dan kerugian bagi masyarakat, hal ini disebabkan oleh serangan dari kumbang Oryctes rhinoceros. Oleh karena itu diperlukan pengendalian salah satunya menggunakan perangkap berferomon. Tujuan penelitian ini adalah untuk mengungkapkan potensi feromon dalam mengendalikan populasi kumbang tanduk (O. rhinoceros). Penelitian ini menggunakan metode observasi dan respons aplikasi feromon untuk memonitoring populasi kumbang O. rhinoceros dan mengetahui intensitas serangan dengan cara menghitung pelepah daun kelapa yang terserang selama dua bulan disetiap minggunya. Kemudian dianalisis secara kuantitatif sederhana dan dihubungkan dengan penurunan produktivitas kelapa. Hasil penelitian menunjukan bahwa penggunaan perangkap berferomon sebagai pengendalian kumbang O. rhinoceros sangat efektif dengan jumlah kumbang yang terperangkap di perkebunan kelapa dalam Desa Pineleng sebanyak 160 ekor dengan perbandingan kumbang jantan dan betina yaitu 1:2. Intensitas serangan kumbang tergolong dalam kategori tinggi mencapai 6-7 ekor/ha dan menyebabkan penurunan produktivitas buah sebesar 45 %.Kata kunci: Oryctes rhinoceros L.; feromon; tanaman kelapa; intensitas serangan.

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