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All Journal HAYATI Journal of Biosciences Media Veteriner Jurnal Ilmu Dasar Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) BERITA BIOLOGI Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY Biosaintifika: Journal of Biology & Biology Education Makara Journal of Science
ENDANG TRI MARGAWATI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Mathematics Veterinary Other

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Effect of maturation periods and leukaemia inhibitory factor on in vitro bovine embryo development Endang Tri Margawati
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 3 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (612.166 KB) | DOI: 10.14334/jitv.v1i3.26

Abstract

The period of in vitro maturation,~20 vs 24 hours) with of without supplementation of :,~uk_ni;ia inhib toryy factor (LIF) (0, 500, 1000 or 2000 U/ml) was studied on bovine embryo development in vitro in a 2 x 4 factotial experiment w id oestgnoo i, a randomized block design . A total of 870 bovine oocytes were used . Besides embryo development, cell numbers of blastocysts v. : ;re also co , mted in order to study the quality of the embryos . Oocytes were matured in a modified TCM199 medium containing 10 ug/nil of FS-14 and LP., I 1~ t1L rstradiol, fertilized in TALP and cultured in SOF/AABSA medium. There was no interaction between maturation periods and LIF doses on embryo development (P>0 .05). Maturation periods, however, affected (P<0.05) blastocyst rates but did not for cleavage o, oocytes and the percentage of oocytes that developed into blastocysts . LIF doses during in vitro maturation did not affect embryo development (P>0 .05) . Cell numbers of blastocysts were also not affected by maturation periods and LIF doses (P<0 .05), however 20 h in vitro maturation and supplementation of LIF doses tended to increase the cell numbers. This study suggests that 20 h maturation increases blastocyst rates and that supplementation with LIF during maturation does not affect the quality of embryos produced in vitro.
Metode Sensitif untuk Identifikasi Pencemaran Babi pada Makanan Tanpa Diolah dengan Teknik Amplifikasi PCR Endang Tri Margawati; Muhamad Ridwan; Indriawati Indriawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.117

Abstract

Pada era globalisasi akhir-akhir ini, tidak mungkin menghindar dari masuknya bahan makanan olahan atau tanpa diolah dari luar negeri. Tujuan penelitian ini yaitu menguji sensitivitas (kerentanan) teknik PCR untuk deteksi kandungan rendah kontaminan daging babi pada daging sapi mentah. Sebanyak 5 tingkat kontaminan daging babi (0,05; 0,10; 0,15; 0,20 dan 0,25%) dalam 5 gram berat total campuran daging telah diuji. Ke lima campuran daging dan 100% daging sapi serta 100% daging babi, dikoleksi DNA nya menggunakan kit DNA (QIAGEN). Satu pasang primer spesifik untuk porcine Leptin digunakan dalam amplifikasi DNA dengan kit PCR. Suhu annealing ditentukan dengan optimasi PCR terlebih dahulu. Dua siklus PCR (25 dan 35) diaplikasikan dalam amplifikasi. Produk PCR divisualisasi pada 1020% gradient PAGE untuk spesifik porcine Leptin. Hasil menunjukkan bahwa ukuran potongan Leptin (152pb) telah teridentifikasi pada ke lima sampel sampuran maupun pada control positif (pork), namun tidak terindikasi pada kontrol negatif (daging sapi). PCR dengan 35 siklus menghasilkan tampilan pita lebih baik dari 25 siklus. Studi ini menyarankan bahwa PCR dengan 35 siklus dapat digunakan sebagai metode cepat untuk identifikasi pencepamaran daging babi dengan dengan tingkat sensitivitas pencemaran daging babi sampai 0,05%.
Expression and Characterization of Recombinant Protein of J-SU pGEX either by Single or Double Cell Lysis Endang Tri Margawati; Muhamad Ridwan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2579

Abstract

Penelitian ini dimaksudkan untuk optimasi produk protein rekombinan Superficial Unit dari virus Jembrana (JSU) yang dieksperikan melalui pemecahan sel secara tunggal dan ganda dengan sistem pGEX dalam skala flask 100ml media kultur. Dua metode pemecahan sel yang digunakan yaitu Freeze and Thaw (FT) sebagai pemecahan tunggal dan gabungan FT dan Sonikasi sebagai pemecahan ganda. Sel inang (E. Coli pembawa konstruk JSU pGEX) ditumbuhkan dengan induksi IPTG pada 37oC dengan pengocok berkecepatan 200rpm sampai mencapai kepadatan sel 0,8. Sel atau pelet dikoleksi dengan sentrifugasi, pelet dipecah dengan 2 perlakuan pemecahan sel tunggal dan ganda. Hasil pemecahan sel disentrifugasi untuk dikoleksi peletnya sebagai inclusion body. Solubilisasi dilakukan terhadap inclusion body dengan solubilisasi buffer dan diperoleh substrat protein JSU kemudian dimurnikan melalui Gluthation sepharose 4B (500μl resin) dengan metode batch capture. Hasil karakterisasi dengan SDS PAGE dan Western Blotting menunjukkan ukuran protein JSU pGEX yang tepat yaitu 60kDa pada kedua sistem pemecahan sel. Namun demikian, pemecahan sel secara tunggal menghasilkan protein murni JSU pGEX lebih besar (0.812ng/ul) dibanding pemecahan sel secara ganda (0.486ng/ul). Dari penelitian ini dapat disimpulkan bahwa protein rekombinan JSU pGEX terekspresi lebih baik dengan metode pemecahan sel Freeze and Thaw.
Producing the Greatest Good for Greatest Numbers-Implementation of Utilitarianism Principle: The Case Study of Producing Recombinant Protein of JDV Endang Tri Margawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2729

Abstract

Advanced technology in molecular biology often uses microorganism, consequently, researcher should have a responsibility in producing of laboratory products safely both for human and their environment. This presentation was intended (1) to report recombinant protein research in the Jembrana Disease Virus (JDV); (2) to identify relevancy of the ethics towards the research of recombinant protein and (3) to discuss relationship of utilitarianism principle with the development of the recombinant protein. The Jembrana disease is an infectious virus caused by a virus classified as retrovirus of Retrovidae family. The disease only attacks Bali cattle (Bos javanicus) that caused about 20% mortality rate. Up to present, crude vaccine from lymph organ of acute infected Bali cattle is often used for vaccination. Development of the Jembrana vaccine was attempted to increase the availability of qualified Jembrana vaccine by recombinant DNA approaches subsequently could be used as vaccine substances. This article was presented with much bioethics issues in associated with recombinant protein research and other examples of related research which use micro-organism in their investigation. It is expected that bioethics could be a restrain for researchers who deal with advanced technology in their investigation.
Confirmation of Existing Insulin-like Growth Factor-1 Gene Associated with Growth and Milk-Production Traits and Genetic Diversity in Buffalo Margawati, Endang Tri; Volkandari, Slamet Diah; Indriawati,; Thalib, Chalid
Makara Journal of Science Vol. 21, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Insulin-like growth factor-1 (IGF-1) gene plays an important role in the endocrine system of animals by regulating nutrient metabolism, growth, and milk production. There have been extensive molecular genetics research studies on cattle but less studies have focused on buffalo (Bubalus bubalis). This study aimed to confirm the association of IGF-1 gene in swamp or river buffalo (B. bubalis spp.) with growth and milk production traits. DNA samples were obtained from 12 buffalos (eight swamp buffalo and four river buffalo). One Bali cattle (Bos javanicus) was included as an outgroup (control). The eight swamp buffalo originated from East Nusa Tenggara (n = 1), Baluran, East Java (n = 4), and Banyuwangi, East Java (n = 3), while the four river buffalo originated from Sei Putih, Medan of North Sumatera. All DNA samples were amplified using an IGF-1 primer for 30 cycles, and amplicons were visualized on 1% agarose gel. Five of the 13 samples were sequenced to determine nucleotide sequence variation between the swamp and river buffalo. The results revealed that the size (225–231 bp) of all the fragments was in in accordance with that of IGF-1. There was not found genetic variation among the buffalo samples. The results indicate that buffalo samples bear growth and milk production traits.
The Application of UTY and SRY Molecular Markers for Determination of Unknown Sex Samples in Bali Cattle Indriawati, Indriawati; Volkandari, Slamet Diah; Margawati, Endang Tri
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.781 KB) | DOI: 10.19184/jid.v21i1.9333

Abstract

An investigation involving large number of animals is often resulting incomplete or in accurate information such as animal parentage, or misidentify on sex due to unlabeled sex samples. A PCR method by applying Y chromosome markers (UTY and SRY) facilitates in determination of unknown sex problem. This study was intended to determine sex from unlabelled sex of blood samples by applying PCR method using a pooled-DNA template. Twenty five of unknown sex blood samples from Nusa Penida, Bali were used in this study. The samples were plotted into 5 pooled-DNA whith each pool DNA consisted of 5 individuals DNA. Two pairs of sex primers, UTY (58oC) and SRY (60oC) with 35 cycles were applied to amplify the samples. The result showed there was only one pooled-DNA (P4) amplified by UTY (484bp). Whereas re-PCR of the positive pooled-DNA (P4) using SRY primer, only one out of 25 samples determined as male Bali cattle (325bp). This finding suggests that UTY and SRY primers are suitable for sex determination and the pooled-DNA could be used as an efficient PCR method both in consumables and PCR process for sex determination. Keywords: Determination, sex, unknown sample, pooled DNA, Bali cattle.
Determining the FecXE Allele in Batur Ewes (Ovis aries) of Indonesia Pintaka Bayu Putra, Widya; Margawati, Endang Tri; Samsudewa, Daud
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 10, No 1 (2023): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v10i1.28654

Abstract

Batur sheep (Ovis aries) is one of the Indonesian native sheep that originated from Banjarnegara Regency. This study aimed to detect the FecXE allele of Bone Morphogenetic Protein 15 (BMP15) in Batur ewes using the sequencing method. A total of six (6) Batur ewes were randomly selected for investigation. The results showed that three (3) ewes were detected as carrier animals (FecX+/ FecXE), and the other ewes were wild-type animals (FecX+/FecX+). Unfortunately, this study did not observe a mutant animal (FecXE/FecXE). It can be concluded that the presence of the FecXE allele in Batur ewes may affect the prolificacy trait since previous studies reported that heterzygous ewes have the largest litter size traits than in wild-type ewes. Keywords: Batur sheep, BMP15 gene, litter size, sequencing,
Detection of the TYR Gene as a Candidate Causal Mutation for Albinism in Taro Cattle Reza, Muhammad Aulia; Noor, Ronny Rachman; Jakaria, Jakaria; Margawati, Endang Tri
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.12

Abstract

Bali Cattle are known as cattle with a unique coat color pattern. At Taro village was found different coat colors of Bali cattle, namely Taro cattle perform white coat color. The TYR gene has a vital role in producing coat color. This research was aimed to determine mutations that caused of the white color in Taro cattle. Two methods of PCR and PCR-RFLP were applied in this study. PCR method followed by sequencing used 29 DNA samples consisted of 17 Taro cattle, 12 DNA samples of 3 breeds cattle as comparison while RFLP method applied 56 DNA samples (17 Taro; 31 wild Bali; 4 Simental, 4 PO/Ongole descendant). All DNA were collected from fresh blood, and extracted by using DNA Extraction Kit. Amplification of TYR (exon 5) gene was conducted using forward and reverse primers to get polymerase chain reaction products with lengths of 320 bp and sequenced with Sanger method (services). PCR-RFLP was conducted with enzyme restriction of AciI. Determination of TYR gene diversity was analyzed through sequencing data. TYR gene sequencing was analyzed by using Bioedit and Mega11 software to identify single nucleotide polymorphisms (SNPs). SNP genotyping was analyzed from PCR-RFLP. The results showed that the TYR gene (exon 5) of Taro cattle was found with 320 bp length. SNP was obtained at one position c.1467 T>C. This SNP has never been reported yet on the ensemble website. This finding suggests that a specific genetic marker for albinism in Bali cattle needs to be more explored
Co-Authors Chalid Thalib Daud Samsudewa HARIMURTI MARTOJO Heni Utami Ningsih, Heni Utami HERMAN WILLEM RAADSMA Indriawati Indriawati Indriawati, Indriawati, Indriawati Indriawati, Indriawati Indriawati, Indriawati Jakaria Jakaria Martien Herna Susanti Muhamad Ridwan Muhammad Ridwan Pintaka Bayu Putra, Widya Reza, Muhammad Aulia Ronny Rachman Noor Salfia, Mega Slamet Diah Volkandari, Slamet Diah SUBANDRIYO SUBANDRIYO Tatag Bagus Putra Praakarsa, Tatag Bagus Putra