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All Journal Journal of Tropical Soils BioWallacea Journal of Biological Research Indonesian Journal of Biotechnology
Erni Martani
Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Poly-β-Hydroxybutyrate (PHB) Production By Amylolytic Micrococcus sp. PG1 Isolated From Soil Polluted Arrowroot Starch Waste Margino, Sebastian; Martani, Erni; Prameswara, Andriessa
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Abstract

Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB ispotential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. Thedevelopment of PHB production using starch as an alternative carbon source has been conducted to reducethe dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processingsite were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of thestudy showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB rangedfrom 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 andafter optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubationtime. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed asMicrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicatedas poly-β- hydroxybutyrate (PHB)
Superoxide Dismutase of Micrococcus sp. S2 and Its Involve in Paraquat Detoxification Margino, Sebastian; Martani, Erni; Magdalena, Medhina
Indonesian Journal of Biotechnology Vol 12, No 1 (2007)
Publisher : Universitas Gadjah Mada

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As an active ingredient of herbicide, paraquat will induce formation of superoxide radicals. The previousresearch succeeded in isolating paraquat degrading bacteria from peat soil, Micrococcus sp. S2, that tolerant to highconcentration of paraquat. An anti-oxidative enzyme, namely superoxide dismutase (SOD, EC.1.15.1.1), wasbelieved to be responsible for the paraquat tolerance. This research was conducted to study the characteristic of theSOD synthesize by Micrococcus sp. S2 and its ability on neutralize superoxide which arise from paraquat reoxidation.To observe the effect of paraquat on Micrococcus sp. S2, the bacteria was grown in 10% Luria Bertani brothmedium amended with several concentrations of paraquat, from 0 (control) up to 100 mg/ml. Within incubationtime of 72 hours, bacterial growth, activity of superoxide dismutase and paraquat residue were analyzed. Theisozymes of superoxide dismutase were distinguished using two kinds of specific inhibitor, namely HO and KCN. 2 2The results showed that paraquat significantly inhibit the growth of Micrococcus sp. S2. The higher paraquatcocentration in the medium caused the higher growth inhibition. However, the bacteria is still survive in the mediumcontaining toxic herbicide, and this ability was suggested related to superoxide dismutase activity in removing thesuperoxide radicals. Analysis using gel electrophoresis indicated that at least three types of SOD isozyme weresynthesized by Micrococcus sp. S2; they were Ferri-SOD (Fe-SOD), Mangani-SOD (Mn-SOD), and the last one wassuspected to be the Cupro Zinc-SOD (CuZn-SOD). The Mangani-SOD was suspected to play an important roles ondetoxifying superoxide which arise from paraquat oxidation.Keywords : Micrococcus sp.S2, paraquat, superoxide dismutase, isozymes
PENGHASILAN BIOPLASTIK OLEH ISOLAT INDIGENUS Bacillus sp. AMILOLITIK DENGAN SUBSTRAT PATI SUWEG (Amorphophallus campanulatus) Margino, Sebastian; Sari, Rarat Mulat; Martani, Erni
Jurnal BioWallacea Vol 2, No 1 (2015): Bodiversitas
Publisher : Jurnal BioWallacea

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bioplastic that was synthesized by bacteria. Glucose as the main carbon source for PHBproduction but its cost was expensive, so we need to substitute with another substrate,including starch.The ability of amylolytic bacteria hydrolyzed starch into simple sugars andconverted to PHB can minimize the costs of PHB production. This research aim was to findout amylolitic bacterial isolates and produce PHB using the selected isolate. A total of 52bacteria were isolated using starch mediumfrom soil sample around Amorphophalluscampanulatus plant. Twenty eight among of them had amylase activity, and eleven among ofthem had amylase activity value equal or more than 2 point.The next selection was donebased on the specific amylase activity and found that five of those isolates had specificamylase activity more than 10 DUN/mg.Final selection was done based on PHB productionand found that isolate E5 had the high amylase activity and PHB production. PHB productionused isolate E5 in optimum conditions (inoculum concentration 10 %, elephant foot yamstarch 3 %, medium pH 7, temperature 30 °C, agitation 125 rpm, and 48 hour incubationtimeshowed that percentage of PHB increased from 9,5% to 14,5%. Characterization andidentification showed that isolate E5 closed to the genus Bacillus sp.Keywords : Amylolytic Bacillus sp., poly-β-hidroxybutyrate, elephant foot yam starch.
Isolation and Selection of Rhizobium Tolerant to Pesticides and Aluminum from Acid Soils in Indonesia Martani, Erni; Margino, Sebastian; Indradewa, Didiek; Supriyo, Agus
JOURNAL OF TROPICAL SOILS Vol. 16 No. 1: January 2011
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2011.v16i1.47-54

Abstract

Application of Rhizobium as inoculum in acid soil requires specific characters, namely high tolerance to pesticide residues, soil acidity, and high concentration of Aluminum. This study was conducted to isolate Rhizobium having these characters. Inspite of acid soils from Kalimantan, Sumatra, Sulawesi and Java; root nodules of legumes planted in those regions were used as source of isolates. Rhizobial isolation was done using direct isolation andenrichment technique. A paper disc diffusion technique was used in selecting tolerance to pesticides. The selected isolates were examined the tolerance to pH, Al, and ability to form root nodule with soybean. From soil analysis, it could be seen the correlation between pH value and Al concentration. It means that the lower pH value the higher Al concentration. The number of Rhizobium isolates and its tolerance to paraquat was depended on soil type. From 173 strains of isolated Rhizobium, 24 strains were tolerance to pesticides and Aluminum. They were able to grow in wide range of pH, namely 3 – 8, or some of them in 5 - 8. Around 92% of the selected bacteria could form root nodules with soybean plant in different number and size. Hopefully, these isolates can be applied in the pesticide polluted agricultural lands, especially in acid soils with high concentration of Al, and it can also increase soybean production.Keywords: Acid soils, aluminium, Rhizobium, pesticides
Co-Authors Agus Supriyo, Agus ANDRIESSA PRAMESWARA Bostang Radjagukguk Elisa Nurnawati Indradewa, Didiek Irfan D. Prijambodo, Irfan D. Kurniawan Wibowo, Kurniawan Medhina Magdalena, Medhina Sari, Rarat Mulat Sarto Sebastian Margino Yanisworo Wijayaratih, Yanisworo