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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Science and Technology Indonesia Jurnal Penelitian Sains IJEMS (Indonesian Journal of Environmental Management and Sustainability) QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Berkala Penelitian Hayati Sriwijaya Bioscientia Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat BIOVALENTIA: Biological Research Journal Jurnal Pepadu Jurnal Manusia dan Lingkungan

Elisa Nurnawati

Biology Department, Faculty Of Mathematics And Natural Sciences, Sriwijaya University, Indonesia

Author-ID : 3516002

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Isolasi dan Karakterisasi Jamur Pendegradasi Katekin dari Seresah Pinus Nurnawati, Elisa; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2003): October 2003
Publisher : Universitas Atma Jaya Yogyakarta

Isolation of catechin-degrading fungus from pine litter samples was done using minimal medium that containing catechin as sole carbon and energy source.Â A total of 53 isolates were chosen to represent different colonial types of catechin degrading-fungus. The isolates were screened for their ability to degrade catechin in three stages. The first stage of screening was based on their ability to grow on solid medium containing 2 mM, and as a result, 28 isolates were selected.Â The second stage of screening on the same medium but containing 4 mM of catechin resulting in 14 selected isolates. The third stage screening was based on their mean growth rate constant (k), instantaneous growth rate constant (m) and generation time (g) on minimal medium containing 4 mM catechin. The result showed that four isolates (D9, K2, K11, and S11) were the best catechin degradator. Further growth kinetic studyÂ (k, m ,and g) of selectedÂ isolatesÂ Â indicated thatÂ D9, K2, and S11 grew well on the medium containing 40 mM, butÂ K11 was inhibited by concentration of higher than 10 mM. Catechin biodegradation process was determined by following the decrease of catechin concentration on liquid medium. It was found that isolate K2 had higher ability to degrade catechin than the isolate K11. Finally, the four selected isolates from the third stage were characterized in terms of macroscopic, microscopic and phenotypic characters and identified. The result of the study showed that the isolates D9, K2 and S11 were identified as member of Aspergillus niger group. The isolate D9 was very similar to isolate S11, while the isolate K2 was found to be the most similar with Aspergillus niger van Tiegh. IFO 6341. The isolate K11 was assigned to be member of the genus Trichoderma.

POTENCY AND ACTIVITY OF SECONDARY METABOLITE OF Trichoderma harzianum AC1(b) J2 INHIBITOR GROWTH Colletotrichum capsici IPBCC 13.1098 Nurkayah, Nurkayah; Nurnawati, Elisa; Widjajanti, Hary
BIOVALENTIA: Biological Research Journal Vol. 5 No. 1 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Trichoderma harzianum is a fungus that can produce secondary metabolites which able to inhibit the growth of Colletotrichum capsici. a pathogenic fungus causing anthracnose in plants. the Aims of this research were to obtain and analyze the ability of secondary metabolites of Trichoderma harzianum AC1 (b) J2 isolated from Scleria poaeformis. to inhibit the growth of Colletotrichum capsici IPBCC13.1098. The secondary metabolite eluat of Trichoderma harzianum were tested for the antifungal activity toward Colletotrichum capsici IPBCC 13.1098. Fractionation was carried out by Column Chromatography and produced 31 eluat. The secondary metabolite eluat of Trichoderma harzianum with the highest inhibition zone diameter was eluat 1 with 8.4 mm in diameter. The secondary metabolite eluat with the highest value of inhibition zone was carried out by MIC test and thin layer chromatography (TLC). Based on the MIC results, the minimum inhibitory concentration of the secondary metabolite of Trichoderma harzianum to fungus Colletotrichum capsici was 250 ppm with inhibition zone diameter was 0.10 mm. The results of TLC showed orange spots on the TLC plate that indicated alkaloite compounds.

EXPLORATION OF ENDOPHYTIC FUNGI OF DRAGON SCALEâ€™S FERN (Pyrrosia piloselloides (L.) M.G. Price) AS AN ANTIBACTERIAL SOURCES Asiandu, Angga Puja; Widjajanti, Hary; Nurnawati, Elisa
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Endophytic fungi are fungi which live inside the host plant tissue and have been undergone a horizontal gene transfer process. Endophytic fungi are able to synthesize the same bioactive compounds which synthesized by their host plants. The host plant used in this research was dragonâ€™s scales fern (Pyrrosia piloselloides (L.) M.G. Price). Dragonâ€™s scales fern produces various of bioactive compounds which used as antibacterial agents such as polyphenols. This research was aimed to obtain endophytic fungi isolates from trophophyll fronds and sporophyll fronds of dragonâ€™s scales fern, to determine the antibacterial activity of the secondary metabolite extracts of endophytic fungi, to determine the Minimum Inhibitory Concentration (MIC), to determine the characteristics of the endophytic fungi isolates which potentially as antibacterial source. Based on the research, 13 endophytic fungi isolates were obtained from dragonâ€™s scales fern fronds consist of 5 isolates from trophophyll fronds and 8 isolates from sporophyll fronds. The antibacterial activity test showed that the extract of secondary metabolites of the isolate DTP2 had the highest inhibition zone diameter against E.coli 14.82 Â± 4.05 mm, DTP4 against S.aureus 8.80 Â± 0.03 mm and DSP4 against S.dysentriae 10.15 Â± 0.36 mm. MIC of ethyl acetate extracts of secondary metabolites of isolate DTP2 against E.coli was 125 Âµg/mL, DTP4 against S.aureus was 125 Âµg/mL and DSP4 against S.dysentriae was 31.25 Âµg/mL. The endophytic fungi isolate DTP2 identified as Aureobasidium melanogenum, DTP4 identified as Penicillium alliisativi and DSP4 identified as Aspergillus flocculosus.

Pengujian Aktivitas Kitinase dari Bacillus Circulans Untuk Dikembangkan Sebagai Agen Biokontrol pada Penyakit Tanaman Muharni Muharni; Elisa Nurmawati
Jurnal Penelitian Sains Vol 10, No 1 (2007)
Publisher : Faculty of Mathtmatics and Natural Sciences

Bacillus circulans mempunyai kemampuan memproduksi kitinase secara ekstraseluler, aktivitas kitinase ini dapat digunakan untuk menghambat pertumbuhan fungsi patogen karena mempunyai kitin pada dinding selnya. Untuk itu pada penelitian ini dilakukan pengujian aktivitas kitinase baik secara kualitatif maupun kuantitatif,s erta kemampuan dalam menghambat pertumbuhan fungi patogen pada tanaman. Berdasarkan hasil yang telah didapatkan dikethui bahwa B. circulans memproduksi kitinase tertinggi pada akhir fase eksponensial yaitu pada jam ke 20 sebesar 0,58 U/ml. Hasil uji antagonisme terhadap fungi patogen Fusarium oxysporum dan Rhizoctonia solani dengan zona hambat sebesar 0,65 cm.

Verulensi Jamur Beauveria Bassiana Indigenus Terhadap Spodoptera exigua Hub. Mustafa Kamal; Umi Kalsum; Elisa Nurnawati
Jurnal Penelitian Sains No 17 (2005)
Publisher : Faculty of Mathtmatics and Natural Sciences

Telah dilakukan penelitian tentang virulensi Beauveria bassiana Indigenus terhadap ulat Bawang Merah Spodoptera exigua Hub. Penelitian ini bertujuan untuk mengetahui virulensi Beauveria bassiana isolat Lahat terhadap larva. Spodoptera exiguna dengan melihat daya bunuh isolat terhadap larva. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan 4 perlakuan dan 5 kali pengulangan, dilanjutkan dengan Uji Lanjut Duncan dengan taraf nyata 5%. Waktu infeksi mulai terjadi pada hari ke 2 setelah aplikasi, waktu kematian larva mulai terjadi pada 4 hari setelah aplikasi, dan terjadi pada konsentrasi tertinggi (106 konidia/ml) diantara 2 konsentrasi lainnya (104 konidia/ml dan 105 konidia/ml). Persentase kematian S. Exiguna tetinggi mencapai 76% pada perlakuan 106 konidia/ml, terendah yaitu 0% pada kontrol. Beauveria bassiana dari tanah perkebunan Lahat sudah dapat membunuh larva Spodoptera exiguna pada konsentrasi 104 konidia/ml.

Identifikasi dan Penapisan Aktivitas Antibakteri Beberapa Jenis Tumbuhan di Desa Napal Licin Kawasan Taman Nasional Kerinci Seblat (TNKS) Salni Salni; Harmida Harmida; Elisa Nurmawati
Jurnal Penelitian Sains Vol 11, No 3 (2008)
Publisher : Faculty of Mathtmatics and Natural Sciences

Telah dilakukan penelitian identifikasi dan penapisan aktivitas antibakteri beberapa jenis tumbuhan desa Napal Licin kawasan Taman Nasional Kerinci Seblat (TNKS) dari bulan Maret dampai Agustus 2005. Identifikasi tumbuhan dilakukan di Laboratorium Herbarium Jurusan Biologi FMIPA Universitas Andalas dan pengujian aktivitas antibakteri dilakukan di Laboratorium Genetika dan Bioteknologi FMIPA Universitas Sriwijaya. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis tumbuhan yang ada di Desa Napal Licin, melakukan penapisan aktivitas antibakteri untuk mengetahui jenis-jenis tumbuhan yang berpotensi sebagai sumber senyawa antibakteri. Hasil penelitian menunjukkan penapisan aktivitas antibakteri terhadap 45 jenis tumbuhan, diperoleh 40 jenis tumbuhan mempunyai aktivitas antibakteri. Dari 40 jenis tumbuhan yang aktif terdapat lime jenis tumbuhan yang paling potensial sebagai sumber senyawa antibakteri, yaitu daun dan kulit Calophyllum dasypodum Miq, daun Jambu Bioa {Eugene densiflora (BI.) Duthie}, daun jambu-jambuan (Eugene virens (BI.) K. & V), daun sirih gedebong (Piper aduncum L.) dan sirih merah (Piper miniatum BI.).

Isolasi dan Karakterisasi Jamur Pendegradasi Katekin dari Seresah Pinus Elisa Nurnawati; Langkah Sembiring
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2003): October 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i3.2855

Isolation of catechin-degrading fungus from pine litter samples was done using minimal medium that containing catechin as sole carbon and energy source. A total of 53 isolates were chosen to represent different colonial types of catechin degrading-fungus. The isolates were screened for their ability to degrade catechin in three stages. The first stage of screening was based on their ability to grow on solid medium containing 2 mM, and as a result, 28 isolates were selected. The second stage of screening on the same medium but containing 4 mM of catechin resulting in 14 selected isolates. The third stage screening was based on their mean growth rate constant (k), instantaneous growth rate constant (m) and generation time (g) on minimal medium containing 4 mM catechin. The result showed that four isolates (D9, K2, K11, and S11) were the best catechin degradator. Further growth kinetic study (k, m ,and g) of selected isolates indicated that D9, K2, and S11 grew well on the medium containing 40 mM, but K11 was inhibited by concentration of higher than 10 mM. Catechin biodegradation process was determined by following the decrease of catechin concentration on liquid medium. It was found that isolate K2 had higher ability to degrade catechin than the isolate K11. Finally, the four selected isolates from the third stage were characterized in terms of macroscopic, microscopic and phenotypic characters and identified. The result of the study showed that the isolates D9, K2 and S11 were identified as member of Aspergillus niger group. The isolate D9 was very similar to isolate S11, while the isolate K2 was found to be the most similar with Aspergillus niger van Tiegh. IFO 6341. The isolate K11 was assigned to be member of the genus Trichoderma.

Triacylglycerols produced by biomass of endophytic fungus Neopestalotiopsis surinamensis from the Scurrula atropurpurea Elfita Elfita; Muharni Muharni; Mardiyanto Mardiyanto; Fitrya Fitrya; Elisa Nurmawati; Hary Widjajanti
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 5, No 3 (2020): October 2020
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v5.i3.95

In Indonesia, Scurrula atropurpurea is a medicinal plant known as benalu. Triacylglycerols can be produced by plants, animals, algae, and microorganisms such as endophytic fungi. Triacylglycerols can be used in cosmetics, food, and medicine because they have biological activities such as antitumor, antibacterial, and cytotoxic. Besides, the compound can be used as a biodiesel substitute for triacylglycerols sourced from oil palm. This study aims to isolate and characterize triacylglycerol from biomass of endophytic fungal N. surinamensis from the S. atropurpurea leaves. The compound was isolated and purified by the column chromatography method. The structure of the compound was determined by spectroscopic data ( FTIR, 1H-NMR, and 13C-NMR). Analysis of the spectrum and compared with the literature, the isolated compound is a triacylglycerol.

Phylogenetic Analysis Of Endophytic Fungi Isolate from Bellucia pentamera Naudin Based On ITS rDNA Andika Puspita Dewi; Elisa Nurnawati; Laila Hanum; Hary Widjajanti
Indonesian Journal of Environmental Management and Sustainability Vol. 3 No. 4 (2019): December
Publisher : Research Centre of Inorganic Materials and Complexs

Endophytic fungi can produce secondary metabolites of endophytic fungi isolated from host plants. The purpose of this study is to identify and analyze the sequencing of endophytic fungi isolates with ITS and Beta-tubulin markers and phylogenetic trees. The endophytic fungi isolate DKJ1, DKJ3a, DKJ3c and DKJ4 were successfully isolated from the cardia plant (Bellucia pentamera Naudin) indicated by Aspergillus niger group, Aspergillus fumigatus group and Penicillium sp. The results of the sequencing analysis of isolates DKJ1, DKJ3a, DKJ3c, and DKJ4 were successfully amplified with an annealing temperature of 54ºC with a pair of ITS1-ITS4 primers with a molecular weight of 570 bp and a Beta-Tubulin primer with an annealing temperature of 56.1ºC molecular weight of 550 bp. From the results of identification and analysis of DNA sequencing of endophytic fungi DKJ1, DKJ3a, DKJ3c and DKJ4 with the primary pair of ITS and Beta-tubulin shows that the phylogenetic tree is different from the species obtained. ITS DKJ1 isolates have similarities with the species Aspergillus piperis CBS 112811, ITS DKJ3c has similarities with the species Aspergillus flavus var flavus strain ATCC 16833, ITS DKJ3a has similarities with Penicillium rolfsii strain NRRL 1078 species and ITS DKJ4 has similarities with Penicillium oxalicum NRRL 787 species. Whereas isolate from DKJ1 Beta-Tubulin has similarities with NRRL 4875 Aspergillus tubingensis species, DKJ3c has similarities with species of Aspergillus novoparasiticus strain DTO 223-C4 and DKJ4 has a similarity with Penicillium guaibinensis species. But there are similarities based on Cluster A (Aspergillus Group) and Cluster B (Penicillium Group) on phylogenetic trees.

Potency of endophytic fungi from Nauclea orientalis L. as antioxidant producer Elisa Nurnawati; Hary Widjajanti; Vivi Hendra Sutandar; Maulida Harwati; Eka Amelia; Siti Alharzsa; Nina Tanzerina
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 1 (2021): December 2021
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869//bphjbr.27.1.20216

Nauclea orientalis L. is widely distributed in Indonesia. Secondary metabolites such as flavonoids, polyphenols, and saponins are abundant in the bark of N. orientalis L. These compounds have the potential to act as antioxidants. Endophytic fungi, through genetic transfer and coevolution, can produce the same metabolites as their host plant. As a result, understanding the potential of endophytic fungi from N. orientalis L. to produce antioxidant compounds that can be developed is critical. According to the findings of this study, twelve isolates have the potential to produce secondary metabolites with antioxidant properties. Three isolates had high antioxidant activity: DB2 was identified as Aspergillus minisclerotigenes with an IC50 of 21 g/mL containing tannins, terpenes, and flavonoids, AB3 as Colletotrichum perseae with an IC50 of 31 g/mL containing tannins and terpenoids, and AB1 as Diaporthe tulliensis with an IC50 of 48 g/mL containing tannins. The secondary metabolite group has the potential to be developed into an antioxidant agent

Co-Authors Ady Mara, Ady Afreni Hamidah Agung Bimantara Alfarado, Daniel Ali Djamhuri Andika Puspita Dewi Asiandu, Angga Puja Christina Vivid Handayani Daniel Alfarado Desnelli Desnelli Desnelli Desnelli Eca Desriana Zahwa Eka Amelia Elfita Elfita Endro Setyo Cahyono, Endro Setyo Erni Martani Erni Martani Erni Martani Evi Yuliza Evi Yuliza, Evi Fatma Ferlinahayati Ferlinahayati Fitri Maya Puspita Fitri Maya Puspita Fitrya Fitrya Harmida Harmida Harmida Harmida Harry Widjajanti Hary Widjajanti Hary Widjajanti Hary Widjajanti Hary Widjajanti Heni Yohandini Heni Yohandini Indrawati Indrawati Jurnal Pepadu Laila Hanum LANGKAH SEMBIRING Langkah Sembiring M.Ridha Mauludi Mardiyanto Mardiyanto Maulida Harwati Mega Tiara Muhammad Said MUHAMMAD SAID Muharni Muharni Muharni Muharni Muharni Muharni Mustafa Kamal Niko Erlangga Nina Tanzerina Nita Aminasih Nurkayah, Nurkayah Poedji Loekitowati Hariani Putra Bahtera Jaya Bangun Putri, Hanindita Alifia Rizkiani, Delisa Robinson Sitepu Rosmania, Rosmania Salni Salni Sarno Sarno Sarto Sarto Sarto Sebastian Margino Sebastian Margino Sebastian Margino Sisca Octarina Siti Alharzsa Suheyanto Suheryanto Sutandar, Vivi Hendra Umi Kalsum Widjajanti, Haryati Ahda

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