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All Journal AL KAUNIYAH Riau Journal of Empowerment JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) Unri Conference Series: Community Engagement

Siti Fatonah

Jurusan Biologi FMIPA, Universitas Riau Pekanbaru

Author-ID : 3182067

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Environmental Science Materials Science & Nanotechnology Public Health Social Sciences

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PEMBENTUKAN TUNAS DARI BIJI MANGGIS (Garcinia mangostana L.) ASAL BENGKALIS DENGAN PENAMBAHAN BAP DAN MADU SECARA IN VITRO Mayta Novalida Isda; Siti Fatonah; Lia Novita Sari
Al-Kauniyah: Jurnal Biologi Vol 9, No 2 (2016): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakGarcinia mangostana L. merupakan salah satu spesies dari famili Guttiferae kaya senyawa dari golongan xanthon. Manggis memiliki juvenil yang lama. Salah satu metode yang dapat memecahkan masalah dalam perbanyakan tanaman manggis dalam jumlah yang banyak, bisa sepanjang musim dan menghasilkan tanaman yang seragam yaitu kultur in vitro. Tujuan dari penelitian ini adalah untuk menentukan konsentrasi terbaik antara Benzil Amino Purin (BAP) dan madu terhadap induksi tunas dari eksplan biji manggis yang belah tiga melintang secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan BAP (0;3 mg/L) dan madu (3;6 mg/L) pada media MS (Murashige-Skoog) dengan pengamatan 70 hari setelah tanam (hst). Hasil penelitian menunjukkan bahwa pemberian BAP maupun madu mampu meningkatkan pembentukan tunas. Pemberian BAP dan madu berpengaruh nyata terhadap persentase terbentuknya tunas, waktu muncul tunas, jumlah tunas dan panjang tunas. Jumlah tunas terbanyak terdapat pada perlakuan 3 mg/L BAP sebanyak 20 tunas per biji. Kombinasi perlakuan dengan penambahan 3 mg/L BAP + 3 ml/L madu menghasilkan persentase terbentuk tunas tertinggi (100%); waktu muncul tunas tercepat 12,75 hst; dan panjang tunas tertinggi 1,86 cm. Pada penelitian ini telah berhasil menginduksi tunas dari biji manggis yang dibelah tiga melintang asal Bengkalis dengan penambahan BAP dan madu.AbstractGarcinia mangostana L. is one of the species within the family Guttiferae that rich in compounds from the class of xanthon. Mangosteen has time juvenile problems. One method that can solve the problem in the mangosteen plant propagation which can be produced in a large number in all season and uniform is in vitro culture. The purpose of this study was to determine the best concentration of BAP and honey to induce the formation of shoot from explants of mangosteen seeds split transversely into three pieces by in vitro method. This study used a Completely Randomized Design (CRD) with a treatment of BAP (0;3 mg/L) and honey (3;6 mg/L) on MS medium with 70 days of observation. The results showed that the BAP and honey can improve the formation of shoots. The addition of BAP and honey significantly affected the percentage of the formed shoots, the period of shoots appear, the number of shoots and shoot length. The treatment of 3 mg/L BAP resulted in the most shoots formed as many as 20 shoots per seed. The combined treatment 3 mg/L BAP + 3 ml/L of honey produced 100% of the formed shoots; fastest period of shoots appear (12.75 DAS); and the longest shoot (1.86 cm). Therefore, the addition of BAP and honey to the three transversely-split mangosteen seeds from Bengkalis origin successfully induced the formation of the shoot.

Induksi Akar pada Eksplan Tunas Anggrek Grammatophylum scriptum var. citrinum secara In Vitro pada Media MS dengan Penambahan NAA Dan BAP Mayta Novaliza Isda; Siti Fatonah
Al-Kauniyah: Jurnal Biologi Vol 7, No 2 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Grammatophyllum scriptum var. citrinum is one variant of endangered from habitat destruction due to logging and forest fires and hunts by orchid collectors. The aim of this study was to determine the effect of BAP and NAA concentration in the root orchid propagation G. scriptum var. citrinum. The results showed that the application of BAP and NAA significant effect on the time of formation appeared roots (19 days), the best root number in the treatment of BAP 0.5 mg/l + 1.0 mg/l NAA for (5 pieces) and root length was obtained NAA 1 mg/l and a combination of 1.0 mg/l BAP + 0.5 mg/l NAA respectively 6.66 cm and 7.40 cm.

Potensi Ekstrak Daun Gulma Babadotan (Ageratum conyzoides L.) terhadap Perkecambahan dan Pertumbuhan Paspalum conjugatum Berg. Mayta Novaliza Isda; Siti Fatonah; Rahmi Fitri
Al-Kauniyah: Jurnal Biologi Vol 6, No 2 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

The allelopathic substances are an organic agents which produced and released by a plant that cause alteration on the neighboring plants. This research was aimed to determine the effect of Ageratum conyzoides L. leaf extract and also to determine optimum concentration on seed germination and growth of weed Paspalum conjugatum Berg.. This experiment used to extract concentration 0%, 5%, 10%, 15% and 20%. The experiment showed that germination and growth of Paspalum conjugatum Berg.were reduced and to happen damage by leaf extract of Ageratum conyzoides L. The highest germination, growth inhibition and demage at concentrations of the leaf extract 20% respectively are 80.5%, 63.15% dan 17,72 %.

Induksi Kalus dari Eksplan Daun In Vitro Keladi Tikus (Typhonium sp.) dengan Perlakuan 2,4-D dan Kinetin Marlina Agustina Sitinjak; Mayta Novaliza Isda; Siti Fatonah
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Rodent tuber (Typhonium sp.) belongs to Araceae that has a potential as medicinal plant. This procedure takes a long periode to produce a large quantity of plant. Therefore, an in vitro propagation using callus culture is necessary to solve this problem. This research aimed to determine the best concentration of 2,4-D and kinetin to induce callus of in vitro leaf rodent tuber explants. This research used in vitro leaft explants of rodent tuber with MS medium. The design used is Randomize Group Design with 10 treatment. The results showed that explants could not produce callus, the explants response just a swelling leaf explants. The treatment that could give up to 100% swelling response was 2.4-D 0.5 mg / L dan 1 mg / L and the combination treatment 0.5 mg / L 2.4-D + 0.3 mg /L kinetin, 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1.5 mg / L 2.4-D + 0.5 mg / L kinetin. The treatments that could maintain 66.67% explants growth was the combination 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1 mg /L 2.4-D + 0.5 mg / L kinetin.

Co-Authors Arini, Arini Ennie Cahyadi Fitmawati Fitmawati Indriatsari Indriatsari Isnaini Isnaini L. FIBRIARTI, BERNADETA Lia Novita Sari Marlina Agustina Sitinjak Mayta Novaliza Isda Muhammad Yulis Hamidy Murniati Murniati Nery Sofiyanti Oswati Hasanah Rahmi Fitri Rodesia Mustika Roza Rr. Sri Catur S Shorea Khaswarina Silvera Devi Sri Catur Setyawatiningsih Sujarwati Sujarwati Yusfiati Yusfiati

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