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Isolation and Identification of Biosurfacting Bacteria from the Contact Pond of WWTP Palm Oil Industry Pond Raynaldo Empindonta Sembiring; M. Hasbi M. Hasbi; Budijono Budijono
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractBiosurfactants are surfactants produced by bacteria capable of degrading oil in water. This study aims to obtain bacteria that can produce biosurfactants from the Contact Pond of IPAL palm oil industry PT. Eka Dura Indonesia, Kota Lama, Rokan Hulu Regency, which was held from April to July 2019. The procedure for the implementation, first, the sample was diluted and enriched using BHI (Brain Heart Infusion) media for 24 hours. Based on the results of the study found 3 isolate of bacteria. The three bacteria are bacteria that can produce bio surfactants with an emulsification index of Isolate D1 41.81%, Isolate D2 43.6%, and Isolate D3 32.72%. Keywords: Bio surfactants, Proteus vulgaris, Proteus mirabilis, and Enterobacter aerogenes

Pb ANDCdCONTENTIN (Osphronemus gouramyand Channa micropeltes) FROM THE KOTO PANJANG DAM, RIAU PROVINSI Suhaili Akbar; Budijono Budijono; M. Hasbi M. Hasbi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 7, No 2 (2020): 2 Juli - Desember 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Osphronemus gouramy and Channa micropeltesinhabit the the Koto Panjang Dam, Riau. The presence of anthropogenic activities may produce heavy metal that enter the water and thus accumulate in the organisms living in that area. A study aims to understand the heavy metal content in gill, muscle and kidney of O. gouramy and C. micropeltesliving in that dam, as well as in the water and in the sediment has been conducted in April 2019. Samplings were conducted 2 times, once/ 2 weeks. Results shown that the heavy metal content in O. gouramywere as follows: Pb 0.0873 mg/kg and Cd 0.0426 mg/kg (gill), Pb 0.0684 mg/kg and Cd 0.0394 mg/kg (kidney), Pb 0.0376 mg/kg and Cd 0.0219 mg/kg (muscle). Water quality parameters were as follows: pH 6-6.7, temperature 28-33°C, DO5.42-7.14, brightness 1.5-2.5 m and depth 22-55 m. Based on data obtained, it can be concluded that the heavy metals content in fish, in the water and sediment were relatively low. Key words: Koto Panjang Reservoir, heavy metal, goramy fish

Isolation And Identification Of Biosurfactant Producing Bacteria From The Coastal Area Of Mengkapan Village, Sungai Apit Distict, Siak Regency, Province Of Riau Apriani Adha Nasution; M. Hasbi M. Hasbi; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract The activities of petroleum drilling, ferry ports and leakages of underwater pipeline caused oil pollution in the coastal area of the Mengkapan Village. The presence of oil pollution may trigger gene mutation in specific bacteria and it may produce biosurfactants for degrading the oil. A study aims to investigate the biosurfactantproducing bacteria in the coastal of Mengkapan village was conducted from March to May 2018. There were 2 stations, in the petroleum drilling area (St1) and in the TanjungButon port (St2). Results shown that the highest bacterial density was inthe St2. Morphological and biochemical characeristics shown that there were 2 bacteria species, namely Bacillus sp and Pseudomonas sp present. Emulsification Index of the Bacillus sp was43% and that of the Pseudomonasspwas 54%, indicating that those bacteria were potencial for producing biosurfactant. Keywords : Microorganisms, Biodegradationt, Oil, Emulsification

Pb andCd Content in Suspended Solid from The Water of tThe Sail River Pekanbaru Mhd Hari Pratama Rangkuti; Budijono Budijono; M. Hasbi M. Hasbi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 7, No 2 (2020): 2 Juli - Desember 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractMany anthropogenic activities are conducted along the watershed of the Sail River and thus producing pollutant that may enter the water. As the pollutant may contain heavy metals, the metal trace might be found in the water’s suspended solid. A research aims to understand the Pb and Cdcontent in the suspended solid from the river has been conducted in February-March 2019. Sampling were conducted 3 times, once/ 10 days and samples were taken from 3 stations, namely upstream (Station I), middlepart (Station II) and downstream (Station III). Results shown that the average of suspended solid in theupstream was 149 mg/L), in the middle part was 237 mg/L and in the downstream was 114 mg/L. The average of Pb and Cd content in the suspended solids from the upstream was 0.00046 mg/L and 0.0364 mg/L respectively, from the middle part was 0.0030 mg/L and 0.0303 mg/L. While the average of Pb and Cd content in the TSS from the downstream was 0.0024 mg/L and 0.0248 mg/L respectively. Based on data obtained, it can be concluded that the Sail River waters has been contaminated by Pb and Cd.Keywords :heavy metals, river pollution, anthropogenic waste, Plumbum, Cadmium

Isolation And Identification of Biosurfic Producing Bacteria From Contact Pond Pond WWTP Sludge Roy Astika Simatupang; M. Hasbi M. Hasbi; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 7, No 1 (2020): Edisi 1 Januari - Juni 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis study aims to obtain bio surfactant-producing bacteria originating from the IPAL pond sludge of the palm oil industry at PT. Eka Dura Indonesia, Ujung Batu, Rokan Hulu, Riau Province. This research uses the survey method. Sludge samples are taken from the Contact pond by using a sample bottle, then put into a cooler, and taken to the laboratory. Next, 5 grams of sludge sample was weighed and the dissolution of the sample was carried out using BPS (Buffer Phosphate Saline) media. Furthermore, this sample was enriched with BHI (Brain Heart Infusion) media, then incubated for 24 hours. Furthermore, this sample was diluted using 0.98% NaCl to 10-6 dilution, and counted the number of bacteria growing, using Petrifilm at 10-4, 10-5 and 10-6 dilution. Then the planting and purification of bacteria were carried out using Blood Agar and MacConkey Agar. Then, it was identified using biochemical tests and PCR (Polymerase Chain Reaction) tests. The results found 2 types of bacterial isolates with 2 species producing bio surfactants, Proteus mirabilis, and Enterobacter aerogenes. The DNA rank of the bacterial species that has the highest Emulsification Index, which is 100% Proteus mirabilis.Keywords: Oil contamination, Biochemical Test, PCR (Polymerase Chain Reaction) Test, Proteus mirabilis and Enterobacter aerogenes

Isolation and Identification of Biosurfactant Producing Bacteria from Contact Pond Ponds of Palm Oil Industry Wwtp Elma Damayanti Br Sembiring; M. Hasbi M. Hasbi; Budijono Budijono
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract Biosurfactants are surfactant compounds produced by microorganisms, and have the ability to reduce surface tension, and can break oil. This study aims to obtain biosurfactant producing bacteria from the pond contact ponds of the palm oil industry WWTP in PT. Sawit Asahan Indah, Ujung Batu, Rokan Hulu Regency. This research was conducted in March-May 2019. First, samples were enriched and diluted with BHI media for 24 hours. Then bacteria are identified by biochemical methods and 18S rDNA. The results revealed that the total number of colonies was 8.7 x 106 Cfu / ml. Morphological and biochemical characteristics obtained 3 types of bacteria, namely Proteus vulgaris, Proteus mirabillis, and Escherichia coli. Two of them are biosurfactant bacteria with the Proteus vulgaris emulsification index 58.3%, and Proteus mirabillis 61.6%. Keywords: Biosurfactant, Proteus vulgaris, Proteus mirabilis, Emulsification Index, 18S rDNA, Biodegradation

Pb and Znconcentration in the water, sediment and Tubifex sp living in the Sail River, Pekanbaru Desita Yohana R; M. Hasbi M. Hasbi; Budijono Budijono
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractSail River is one of polluted rivers in Riau. High Pb and Zn concentrations in the water and sediments may affects organisms living in the rivers, including Tubifex. A study aims to understand the Pb and Zn concent in the water, sediment and Tubifexin that river was conducted in October - November 2017. There were 3 stations, in the upstream (St1), in the residential area (St2) and in the downstream (St3). Samplings were conducted 3 times,once/2 weeks. Results indicate that the concentration of Pb in the water, sediment and Tubifex were 0.276 to 0.776 mg/L, 1.249 to 3.643 mg/kg and 3.643 to 5.613 mg/kg. While the Zn concentration in the water, sediment and Tubifex were 0.597 to 0.785 mg/L, 62.165 to 124.329 mg/kg and 83.982 to 94.995 mg/kg respectively. It was concluded that the water, sediments and Tubifex worms from the Sail River were already contaminated with Pb and Zn.Keywords : Heavy metals, polluted rivers, residential area, contaminated

Isolation and Identification of Biosurfactant Producing Bacteria from Anaerobic Pond Mud WWTP Palm Oil Industry Lovi Yona; M. Hasbi M. Hasbi; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract This study aims to isolate and identify the types of biosurfactant producing bacteria in anaerobic ponds WWTP palm oil industry PT. Sawit Asahan Indah. This research was conducted from April to May 2019. Bacteria were isolated and identified biochemically and 16S R DNA method. The number of bacteria obtained is 8,3 x 106 CFU / ml. The results of the study identified three types of bacteria, namely Proteus vulgaris, Proteus mirabilis and Enterobacter aerogenes. The Proteus vulgaris emulsification index is 25%, the Proteus mirabilis emulsification index is 43.3% and the emulsification index Enterobacter aerogenes is 41.6%. This shows that all three bacteria have the potential to produce biosurfactants. Keywords : Biosurfactant, Proteus vulgaris, Proteus mirabilis, Enterobacter aerogenes, Emulsification Index

Kandungan Logam Berat Pb dan Zn Dalam Organ Ikan Baung (Hemibagrus nemurus) dari Dua Danau Oxbow di Kecamatan Siak Hulu, Kampar Hendra Rezki; Budijono Budijono; M. Hasbi M. Hasbi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 7, No 2 (2020): 2 Juli - Desember 2020
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractLubuk Siam Lake and Pinang Dalam Lake are oxbow lakes formed due to the interruption of the Kampar River flow. Fish is one of the aquatic biota which can be used as an indicator of the level of pollution that occurs in the waters. Asian Redtail Catfish (Hemibagrus nemurus) inland fisheries that has economic value and is often caught by fishermen around the lake. The purpose of this study was to determine the content of heavy metals Pb and Zn which accumulate in the skin, liver, and kidney organs of the fish in the two lakes. Organ samples were collected and digested according to standard procedures to determine the concentrations of heavy metals Pb and Zn tested using Atomic Absorption Spectrophotometry (AAS). The results showed that the highest and lowest heavy metals were in the organ of Asian Redtail Catfish in Lubuk Siam Lake, namely Pb (kidney> liver> skin) and Zn (kidney> liver> skin), while for Pinang Dalam Lake, namely: Pb (liver> skin). > kidney) and Zn (kidney> skin> liver).Keywords: Heavy metal, baung fish, organs, Pinang Dalam Lake.

THE EFFECT OF BIOJUK TREATMENT ON DECREASING TOM AND BOD LEVELS CONTAINED ON RIVER SAIL WATER IN PEKANBARU CITY Hawari Putriningsih; Budijono Budijono; M. Hasbi M. Hasbi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTSail River is one of the rivers in the city of Pekanbaru that has been polluted with organic pollutants. This study aims to determine the decrease in TOM and BOD levels by using different bioijuk densities. This research was carried out on the Sail River, in March-April 2019. The method used was an incomplete design (CRD), with 3 treatments of bioijuk density namely P1 (low density), P2 (medium density) and P3 (high density), and each -one with 3 replications. The sampling location was selected in the inlet section (before passing bioijuk) and the outlet section (after passing bioijuk) artificial ditch. The results showed that the highest decrease in TOM levels occurred in P2 (0.046 mg / L) with a decrease in effectiveness (EP) of 95%. The highest decrease in BOD levels was found in P2 (4.66 mg / L) with an EP of 53%. It turns out that the treatment of different bioijuk densities does not have a significant effect on decreasing levels of the TOM and BOD of the Sail River.Keywords: Bioijuk, TOM, BOD, bacteria, effective sperm

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