M. Hasbi M. Hasbi
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The effectiveness of combined anaerob-aerob biofilters and phytoremediation using Eichhornia crassipes for reducing ammonia and TSS content in noodle industry liquid waste Zubaidah Zubaidah; Sampe Harahap; M. Hasbi M. Hasbi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract The noodle industry liquid waste is rich in ammonia and TSS and thus need to be processed before being flown to environment. A research aims to reduce the ammonia and TSS on liquid waste using biofilters has been conducted in March-April 2018.The noodle industry liquid waste (175 liters) wastreated using a batch system that was consisted of2 anaerob and 1 aerob tanks and Eichhrornia crassipes phytoremediation pond. The liquid waste was kept for 10 days in anaerob tank with gravel, sand and fibers media. Then the waste was kept for 7 days in aerob tanks with mangrove charcoal media.Finally the waste was kept for 15 days in a phytoremediation pondthat was completed with Eichhrornia crassipes. By the end of the experiment, the ammonia reduced from 9.123 mg/L to 0.415 mg/L (effectiveness 95.45%)and TSS reduced from 448 mg/L to 85 mg/L (effectiveness 81.02%). The treated waste was used for rearing Cyprinus carpio L. fingerling (4 - 7 mm TL) for 15 days and survival rate of the fish was 85%. Based on data obtained, the combination of anaerob-aerob biofilters and phytoremediationusing Eichhornia crassipes is effective to reduce the ammonia and TSS content in thenoodle industry liquid waste.Keywords: Mangrove charcoal, Organic waste, organic pollutant, waste water, batch system  
Isolation and Selection of Biosurfactant-Producing Bacteria from Sludge Siak River, Around Sungai Duku Port of Pekanbaru Vera Linda Uli Simanjuntak; M. Hasbi M. Hasbi; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractActivities in the Sungai Duku port was causing oil pollutionwhich then trigger gene mutation in specific bacteria.  The bacteria may produce biosurfactants for degrading the oil. A study aims to obtain the biosurfactantproducingbacteria from oil polluted sludge was conducted fromJune to July 2018. Thesludge sample was taken to laboratoryand 2 ml of the sample was mixed with Enrichment media to grow up the bacteria. The bacteria were isolated and purified using Blood Agar and MacConkey media. The purified bacteria were then morphologically studied and biochemicallytested. Morphological and biochemical characteristics shown that there were 2 bacteria species, namely Proteus sp and Enterobacter sp.Emulsification Index of the Proteus sp was 42.5% and  Enterobacter sp was 50%, indicating that those bacteria were potencial for producing biosurfactant.Keywords : Enterobactersp, Proteus sp, Biodegradationt, Oil pollutant, Emulsification Index
Organic Pollution Carrying Capacity of the BatangArau River, Padang, Sumatra Barat Province Muhammad Ridho; Sampe Harahap; M. Hasbi M. Hasbi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT BatangArau River received organic matter from domestic activities conducted around the river. As the load capacity of a river is limited, any load addition may damage the ecosystem in the river. The aims of this study is to determine the capacity of the organic pollution load of the BatangArau River based on the BOD and COD content. This research was conducted in June 2017. There were 5 stations, in the upstream (S1), in the middle of the river (S2 and S3) and in the downstream (S4 and S5). Water samplings were conducted 3 times, once per week. Parameters measured were BOD and COD. The load capacity was calculated using a mass balance method. Results shown that the BOD content was 4.3 mg/L and COD 17.7 mg/L. Based on government standard (PP. 82 of 2001), the river is categorized as polluted and it may not be able to receive any organic matter load addition.Keyword :Mass balance method, pollution load, organic matter, domestic activities
Isolation and Identification of Biosurfactant Producing Bacteria from Contact Pond Pond WWTP Palm Oil Industry Herman Syahputra Tambunan; M. Hasbi M. Hasbi; Eko Purwanto
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractThis study aims to obtain biosurfactant producing bacteria originating from the pond mud of IPAL in the palm oil industry at PT. Sawit Asahan Indah, Ujung Batu, Rokan Hulu, Riau Province. This study uses a survey method. Mud samples are taken from the Contact pond by using a sample bottle, then inserted into the cool box, then taken to the laboratory. Furthermore, 5 gramsof mud samples were weighed and sample dissolution was carried out using BPS (Buffer Phosphate Saline) media. Furthermore, this sample was enriched with BHI(Brain Heart Infusion) media, then incubated for 1x24 hours. Furthermore, this sample was diluted using 0.98% NaCl until a dilution of 10-6, and calculated the number of bacteria growing, using Petrifilm at 10-4, 10-5 and 10-6 dilutions. Then bacterial planting and purification were carried out using the media of Blood Agar and MacConkey Agar. Then, it was identified using biochemical tests andPCR (Polymerase Chain Reaction) tests. The results found 3 types of bacterial isolates with 2 biosurfactant producing species, Proteus mirabilis, and Providencia stuartii. Then it is known the rank ofDNA from bacterial species that have the highest Emulsification Index, namely 100% Proteus mirabilis.Keywords: Oil contamination, Biochemical Test, PCR test, Proteus mirabilis and Providencia stuartii