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All Journal Dental Journal (Majalah Kedokteran Gigi) Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) JPMP
Eddy Hermanto
Bedah Mulut Fakultas Kedokteran Gigi Universitas Hang Tuah
Humanities Dentistry Energy Engineering Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Physics Social Sciences

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Effects of Anadara granosa shell combined with Sardinella longiceps oil on oesteoblast proliferation in bone defect healing process Rima Parwati Sari; Eddy Hermanto; Dinda Divilia; Indra Candra; Wisnu Kuncoro; Tantri Liswanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 1 (2016): March 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.93 KB) | DOI: 10.20473/j.djmkg.v49.i1.p27-31

Abstract

Background: Alveolar bone damage is the most common case in dentistry. One way to fix the bone damage is by using bone graft. Anadara granosa shell is a potential bone substitute since it is rich in calcium which can be processed into hydroxyapatite. The addition of Sardinella longiceps oil rich in omega-3 can modulate inflammation, thus accelerating the healing process. Purpose: This study aimed to determine effects of application of Anadara granosa shell combined with Sardinella longiceps oil on osteoblast proliferation in the healing process of bone defects. Method: The subjects were 32 male rats type Wistar divided into 4 groups (n = 8). Making defect was performed on the right bone of the femurs with a half of the diameter of round Mcisinger® Germany bur sized 18. The first group (K) is a negative control group that was not given anything. The second group (AG) was given Anadara granosa pasta. The third group (AM10) was given Anadara granosa pasta combined with 10% Sardinella longiceps oil. And, the fourth group (AM30) was given Anadara granosa pasta combined with 30% Sardinella longiceps oil. Next, preparations and animal euthanasia were performed on the 7th day after the treatment. The number of osteoblasts then was measured after making preparations for HPA with Hematoxylin eosin staining (HE). Afterward, tabulation of data followed by statistical analysis of Anova and HSD Tukey was carried out. Result: The average number of osteoblasts in Groups K, AG, AM10, and AM30 was 19.00, 34.63, 33.50, and 38.50. The results of Anova test showed a significant difference (p<0.05). Similarly, the results of Tukey-HSD test also showed significant differences (p<0.05) between Group K and all other groups (AG, AM10, and AM30). Nevertheless, there were no significant differences between Group AG and Groups AM10 and AM30, as well as between Group AM10 and Group AM30. Conclusion: The application of the combination of Anadara granosa shell and Sardinella longiceps oil can not increase the proliferation of osteoblasts in the healing process of bone defects.
Grafting effectiveness of Anadara granosa shell combined with sardinella longiseps gel on the number of osteoblast-osteoclast cells Eddy Hermanto; Rima Parwati Sari; Asri Cahyadita Dwi Imaniar; Kevin Anggoro
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 3 (2017): September 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.491 KB) | DOI: 10.20473/j.djmkg.v50.i3.p138-143

Abstract

Background: Bone grafts derived from Anadara granosa shells contain calcium carbonate that possesses bone-healing properties. The combination of Sardinella Longiceps fish oil, containing EPA and DHA, and Anadara granosa shells was assumed to regulate the number of osteoblasts-osteoclasts during the bone-healing process. Purpose: This study aimed to determine the effectiveness of Anadara granosa shell grafts, combined with Sardinella Longiceps fish oil, in the bone-healing process by observing the ratio of osteoblasts-osteoclasts in Rattus novergicus rats. Methods: The Wistar rat subjects (n = 25) were divided into five groups, namely: one untreated group (control), one group treated with bone grafts derived from Anadara granosa shells (P1), and the other three groups treated with a combination of Anadara granosa shells and Sardinella longiceps fish oil at concentrations of 10%, 20%, and 30% (P2, P3, and P4). Then, a wound equivalent in size to half the diameter of a round bur (±1.5mm) was intentionally inflicted on the right femur of all the subjects. The rats were subsequently sacrificed on day 14, their femur in the transversal side being cut before HE staining was completed. Thereafter, the ratio of osteoblasts to osteoclasts was measured by means of a light microscopy. The data was subsequently analyzed using one-way ANOVA. Results: The average number of osteoblasts in all research groups increased, viz: 9.420±0.8044 for control group (K), 12.080±0.79811 for group P1, 20.020±0.7190 for group P2, 25.940±0.7197 for group P3, and 36.280±0.9985 for group P4. Similarly, the number of osteoclasts in all groups subject to analysis also increased, namely: 1.73±0.098 for group K, 2.19±0.305 for group P1, 1.60±0.088 for group P2, 1.60±0.724 for group P3, and 1.80±1.302 for group P4. Moreover, the results of the One-way Anova test confirmed that there were no significant differences in osteoclasts between all research groups (p>0.05). The results of the one-way ANOVA and LSD tests confirmed there to be significant differences (p <0.05) between group K and other treatment groups (P1, P2, P3, and P4). Conclusion: The grafts derived from the combination of Anadara granosa shells and Sardinella longiceps gel can induce the production of osteoblasts, but not in the numbers necessary during the healing processin the femurs in Rattus novergicus rats.
Progressive systemic sclerosis with unilateral osteolysis of the mandible: a unique case report and review Lusi Epsilawati; Chrisna Ardhya Medika; Eddy Hermanto
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Vol 5 No 3 (2021): Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI)
Publisher : Ikatan Radiologi Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jrdi.v5i3.734

Abstract

Objectives: This case report is aimed to discuss case findings of Progressive Systemic Sclerosis (PSS), an overview of the characteristics in the form of osteolysis on one side of the mandible, and a theoretical review. Case Report: A 30-year old male patient came to an oral surgeon after tooth extraction. Clinical extraoral examination revealed hyperpigmentation on the right side of the face. A radiological study showed widening periodontal space on posterior teeth, and the angles of the mandibular arch, the jaw branch and the mandibular condyle neck were dissolved in the form of bone resorption. Conclusion: Characteristics of Progressive Systemic Sclerosis (PSS) in radiographs appear in the form of expansion of the periodontal space and osteolysis of the mandibular angle, branch, and even condyle. This disease is caused by an autoimmune disease that affects the entire body, but it can manifest on one side of the body.
KNOWLEDGE AND INDEPENDENCE IN THE CARE OF COMMUNITY DENTAL AND ORAL HEALTH TO TEACHERS AND PARENTS OF ELEMENTARY SCHOOL STUDENTS Restika Pinasti; Agni Febrina; Kristanti Parisihni; Linda Rochyani; Meinar Nur; Chaterina Dyah Nanik; Onge Victoria; Vivin Ariestania; Arifzan Razak; Yoifah Rizka; Eddy Hermanto; Nafiah; Endah Wahjuningsih
Jurnal Pengabdian Masyarakat Pesisir VOLUME 2 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v2i1.89

Abstract

Kesehatan gigi dan mulut di Indonesia masih merupakan masalah kesehatan yang perlu diperhatikan. Berdasarkan Riskesdas 2018, dilaporkan bahwa prevalensi masalah gigi dan mulut di Indonesia masih sangat tinggi. Sebanyak 57,6 persen orang Indonesia memiliki masalah gigi dan mulut. Hal yang paling memperhatinkan adalah sebanyak 93% anak-anak usia sekolah mengalami masalah gigi dan mulut. Kegiatan ini ditujukan sebagai program sekolah yang komprehensif di Indonesia, yang terdiri dari program 21 hari kesehatan gigi dan mulut pada anak di sekolah. Pelaksanaan Program Kesehatan Sekolah ini mengunakan sistem online melalui aplikasi zoom. Target peserta adalah 5 orang tua dan 5 guru di SD Hangtuah 6 Surabaya. Untuk lokasi dan waktu pelaksanaan adalah Sabtu, 5 November 2022, pukul 08.30 – 11.00 WIB dengan pelaksanaan menggunakan zoom meeting. Hasil analisis statistik menggunakan paired sample t-test nilai signifikansi 0.000, hal ini berarti bahwa terdapat perbedaan yang signifikan pada tingkat pengetahuan menjaga kesehatan gigi dan mulut pada guru pengajar dan orang tua murid di SD Hangtuah 6 sebelum diberi materi DHE dan sesudah diberi DHE melalui “Training of Trainer”. Guru dan orang tua dapat menjadi pioner dalam menyebarluaskan ilmu dan cara menjaga kesehatan gigi dan mulut bagi anak tingkat sekolah, juga untuk mengubah perilaku menjaga kesehatan gigi dan mulut menjadi lebih baik.
Co-Authors Agni Febrina ariestania, Vivin Arifzan Razak Asri Cahyadita Dwi Imaniar Chaterina Dyah Nanik Chrisna Ardhya Medika Dinda Divilia Endah Wahjuningsih Indra Candra Kevin Anggoro Kristanti Parisihni Linda Rochyani Lusi Epsilawati Meinar Nur Nafiah Onge Victoria Restika Pinasti sari, Rima parwati Tantri Liswanti Wisnu Kuncoro Yoifah Rizka