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Effects of Anadara granosa shell combined with Sardinella longiceps oil on oesteoblast proliferation in bone defect healing process Rima Parwati Sari; Eddy Hermanto; Dinda Divilia; Indra Candra; Wisnu Kuncoro; Tantri Liswanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 1 (2016): March 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.93 KB) | DOI: 10.20473/j.djmkg.v49.i1.p27-31

Abstract

Background: Alveolar bone damage is the most common case in dentistry. One way to fix the bone damage is by using bone graft. Anadara granosa shell is a potential bone substitute since it is rich in calcium which can be processed into hydroxyapatite. The addition of Sardinella longiceps oil rich in omega-3 can modulate inflammation, thus accelerating the healing process. Purpose: This study aimed to determine effects of application of Anadara granosa shell combined with Sardinella longiceps oil on osteoblast proliferation in the healing process of bone defects. Method: The subjects were 32 male rats type Wistar divided into 4 groups (n = 8). Making defect was performed on the right bone of the femurs with a half of the diameter of round Mcisinger® Germany bur sized 18. The first group (K) is a negative control group that was not given anything. The second group (AG) was given Anadara granosa pasta. The third group (AM10) was given Anadara granosa pasta combined with 10% Sardinella longiceps oil. And, the fourth group (AM30) was given Anadara granosa pasta combined with 30% Sardinella longiceps oil. Next, preparations and animal euthanasia were performed on the 7th day after the treatment. The number of osteoblasts then was measured after making preparations for HPA with Hematoxylin eosin staining (HE). Afterward, tabulation of data followed by statistical analysis of Anova and HSD Tukey was carried out. Result: The average number of osteoblasts in Groups K, AG, AM10, and AM30 was 19.00, 34.63, 33.50, and 38.50. The results of Anova test showed a significant difference (p<0.05). Similarly, the results of Tukey-HSD test also showed significant differences (p<0.05) between Group K and all other groups (AG, AM10, and AM30). Nevertheless, there were no significant differences between Group AG and Groups AM10 and AM30, as well as between Group AM10 and Group AM30. Conclusion: The application of the combination of Anadara granosa shell and Sardinella longiceps oil can not increase the proliferation of osteoblasts in the healing process of bone defects.
Grafting effectiveness of Anadara granosa shell combined with sardinella longiseps gel on the number of osteoblast-osteoclast cells Eddy Hermanto; Rima Parwati Sari; Asri Cahyadita Dwi Imaniar; Kevin Anggoro
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 3 (2017): September 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.491 KB) | DOI: 10.20473/j.djmkg.v50.i3.p138-143

Abstract

Background: Bone grafts derived from Anadara granosa shells contain calcium carbonate that possesses bone-healing properties. The combination of Sardinella Longiceps fish oil, containing EPA and DHA, and Anadara granosa shells was assumed to regulate the number of osteoblasts-osteoclasts during the bone-healing process. Purpose: This study aimed to determine the effectiveness of Anadara granosa shell grafts, combined with Sardinella Longiceps fish oil, in the bone-healing process by observing the ratio of osteoblasts-osteoclasts in Rattus novergicus rats. Methods: The Wistar rat subjects (n = 25) were divided into five groups, namely: one untreated group (control), one group treated with bone grafts derived from Anadara granosa shells (P1), and the other three groups treated with a combination of Anadara granosa shells and Sardinella longiceps fish oil at concentrations of 10%, 20%, and 30% (P2, P3, and P4). Then, a wound equivalent in size to half the diameter of a round bur (±1.5mm) was intentionally inflicted on the right femur of all the subjects. The rats were subsequently sacrificed on day 14, their femur in the transversal side being cut before HE staining was completed. Thereafter, the ratio of osteoblasts to osteoclasts was measured by means of a light microscopy. The data was subsequently analyzed using one-way ANOVA. Results: The average number of osteoblasts in all research groups increased, viz: 9.420±0.8044 for control group (K), 12.080±0.79811 for group P1, 20.020±0.7190 for group P2, 25.940±0.7197 for group P3, and 36.280±0.9985 for group P4. Similarly, the number of osteoclasts in all groups subject to analysis also increased, namely: 1.73±0.098 for group K, 2.19±0.305 for group P1, 1.60±0.088 for group P2, 1.60±0.724 for group P3, and 1.80±1.302 for group P4. Moreover, the results of the One-way Anova test confirmed that there were no significant differences in osteoclasts between all research groups (p>0.05). The results of the one-way ANOVA and LSD tests confirmed there to be significant differences (p <0.05) between group K and other treatment groups (P1, P2, P3, and P4). Conclusion: The grafts derived from the combination of Anadara granosa shells and Sardinella longiceps gel can induce the production of osteoblasts, but not in the numbers necessary during the healing processin the femurs in Rattus novergicus rats.
Progressive systemic sclerosis with unilateral osteolysis of the mandible: a unique case report and review Lusi Epsilawati; Chrisna Ardhya Medika; Eddy Hermanto
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Vol 5 No 3 (2021): Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI)
Publisher : Ikatan Radiologi Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jrdi.v5i3.734

Abstract

Objectives: This case report is aimed to discuss case findings of Progressive Systemic Sclerosis (PSS), an overview of the characteristics in the form of osteolysis on one side of the mandible, and a theoretical review. Case Report: A 30-year old male patient came to an oral surgeon after tooth extraction. Clinical extraoral examination revealed hyperpigmentation on the right side of the face. A radiological study showed widening periodontal space on posterior teeth, and the angles of the mandibular arch, the jaw branch and the mandibular condyle neck were dissolved in the form of bone resorption. Conclusion: Characteristics of Progressive Systemic Sclerosis (PSS) in radiographs appear in the form of expansion of the periodontal space and osteolysis of the mandibular angle, branch, and even condyle. This disease is caused by an autoimmune disease that affects the entire body, but it can manifest on one side of the body.
KNOWLEDGE AND INDEPENDENCE IN THE CARE OF COMMUNITY DENTAL AND ORAL HEALTH TO TEACHERS AND PARENTS OF ELEMENTARY SCHOOL STUDENTS Restika Pinasti; Agni Febrina; Kristanti Parisihni; Linda Rochyani; Meinar Nur; Chaterina Dyah Nanik; Onge Victoria; Vivin Ariestania; Arifzan Razak; Yoifah Rizka; Eddy Hermanto; Nafiah; Endah Wahjuningsih
Jurnal Pengabdian Masyarakat Pesisir VOLUME 2 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v2i1.89

Abstract

Kesehatan gigi dan mulut di Indonesia masih merupakan masalah kesehatan yang perlu diperhatikan. Berdasarkan Riskesdas 2018, dilaporkan bahwa prevalensi masalah gigi dan mulut di Indonesia masih sangat tinggi. Sebanyak 57,6 persen orang Indonesia memiliki masalah gigi dan mulut. Hal yang paling memperhatinkan adalah sebanyak 93% anak-anak usia sekolah mengalami masalah gigi dan mulut. Kegiatan ini ditujukan sebagai program sekolah yang komprehensif di Indonesia, yang terdiri dari program 21 hari kesehatan gigi dan mulut pada anak di sekolah. Pelaksanaan Program Kesehatan Sekolah ini mengunakan sistem online melalui aplikasi zoom. Target peserta adalah 5 orang tua dan 5 guru di SD Hangtuah 6 Surabaya. Untuk lokasi dan waktu pelaksanaan adalah Sabtu, 5 November 2022, pukul 08.30 – 11.00 WIB dengan pelaksanaan menggunakan zoom meeting. Hasil analisis statistik menggunakan paired sample t-test nilai signifikansi 0.000, hal ini berarti bahwa terdapat perbedaan yang signifikan pada tingkat pengetahuan menjaga kesehatan gigi dan mulut pada guru pengajar dan orang tua murid di SD Hangtuah 6 sebelum diberi materi DHE dan sesudah diberi DHE melalui “Training of Trainer”. Guru dan orang tua dapat menjadi pioner dalam menyebarluaskan ilmu dan cara menjaga kesehatan gigi dan mulut bagi anak tingkat sekolah, juga untuk mengubah perilaku menjaga kesehatan gigi dan mulut menjadi lebih baik.
The Difference in Blood Pressure Using the Open Extraction Method Before and After Local Anesthesia with Lidocaine + Adrenaline 1:80,000 at RSGM Nala Husada Mecthildis Vianeys; Eddy Hermanto
Denta Journal Kedokteran Gigi Vol 19 No 1 (2025): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v19i1.5

Abstract

Background: Local anesthesia is the first step before open method extraction. The local anesthesia contains 2% lidocaine and adrenaline in a 1:80,000 ratio to extend its duration and reduce the risk of toxicity. However, the use of vasoconstrictors may lead to a spike in blood pressure. Objective: To determine the difference in blood pressure before and after the administration of lidocaine + adrenaline in a 1:80,000 ratio for open method extraction at RSGM Nala Husada Surabaya. Materials and Methods: Blood pressure was measured before and after the administration of local anesthesia containing lidocaine + adrenaline in a 1:80,000 ratio for open method extraction, following the inclusion criteria with a sample size of 21. Blood pressure was measured using an "Omron HEM-FL31" digital sphygmomanometer. Data analysis was performed using a paired t-test. Results: The paired t-test results showed a significant difference in systolic blood pressure (p<0.05), but no significant difference in diastolic blood pressure (p>0.05) before and after the administration of local anesthesia for open method extraction patients. The systolic blood pressure showed the highest value after the administration of local anesthesia (122.95 ± 14.59). Conclusion: Blood pressure showed a significant difference before and after the administration of local anesthesia containing lidocaine + adrenaline in a 1:80,000 ratio for open method extraction at RSGM Nala Husada Surabaya.
Closed reduction and drainage incision for the treatment of neglected and infected mandibular fractures Hermanto, Eddy; Laihad, Fanny Margaretha; Pranoto, Amelia Elizabeth; Elidasari, Monika; Tantra, Ivan; Sarianoferni; Damayanti, Dian Widya
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p197-201

Abstract

Background: Jaw fracture is the most common facial fracture in oral and maxillofacial bone and is usually caused by trauma. The fracture itself could lead to infection due to bone and tissue damage, which is the port of entry for microorganisms. Fracture-related infection (FRI) in the patient discussed in this study manifested as a submandibular abscess. The goals of fracture treatment were achieving the anatomic reduction of the fracture line and regaining acceptable occlusion. There are two methods for treating mandibular fractures: the closed method, also called conservative treatment, and the open method, which requires advanced surgery. Closed method treatment uses a maxillomandibular fixation (MMF) device in order to reduce and immobilize fracture fragments. Treatment of FRI should use a multidisciplinary approach to achieve an outstanding result, such as wound debridement, antimicrobial therapy, and implant retention. Purpose: The purpose of this article is to report a case of neglected mandibular fracture with a submandibular abscess, which was treated with a combination of the closed reduction method and incision drainage. Case: A 25-year-old female visited Nala Husada Hospital because of a submandibular abscess on the neglected mandibular fracture of the right parasymphysis and left corpus. Case management: The case was managed using an arch bar in the mandible and an eyelet in the maxilla while continuing with MMF and an extra oral drainage incision. Conclusion: Combination therapy (MMF and incision drainage) was needed to treat this case because of the occurrence of a submandibular abscess due to a neglected mandibular fracture.
Characteristics of biphasic calcium phosphate scaffold formed by Anadara granosa shell synthesis Sari, Rima Parwati; Sari, Maharani Dewinta; Ramanindia, Andiani; Fitri, Karimatul; Susanto, Michelle Rosalind; Pratiwi, Safira Agnes; Yusuf, Diajeng Ayu Permatasari; Prananingrum, Widyasri; Ariestania, Vivin; Widaningsih; Apsari, Anindita; Widyastuti; Brahmanta, Arya; Hermanto, Eddy
Dental Journal (Majalah Kedokteran Gigi) Vol. 59 No. 1 (2026): March
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v59.i1.p78-86

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Background: Bone augmentation requires biomaterials that can trigger bone regeneration, especially when treating alveolar bone defects. Biphasic calcium phosphate (BCP) can induce cell proliferation and differentiation. Anadara granosa shell can be synthesized into BCP and made into a scaffold with the addition of gelatin. Purpose: To determine the optimal BCP ratio from A. granosa shell synthesis and to establish the material characteristics. Methods: Anadara granosa shells were synthesized using the hydrothermal method at 200°C for 12 and 18 hours, followed by particle size analysis. Next, the resulting powder was made into scaffolds by adding gelatin through the freeze-drying method, and a series of observations were performed regarding its morphology, pore size, porosity, compressive strength (CS), and cell viability. The data were analyzed using an independent t-test, with p < 0.05. Results: Synthesis of A. granosa shells for 12 hours produced a BCP ratio of 69:20 with 11% calcite content and a particle size of 3,343.00 ± 445.25 nm, whereas synthesis for 18 hours produced a ratio of 22:78 with a particle size of 919.96 ± 375.77 nm. In scaffold manufacture, the BCP18 pore size (26.56–145.45) was smaller than that of BCP12 (19.72–309.09), the porosity level was lower (61.49% vs. 81.98%, respectively), and the CS was higher (4.66 ± 1.12 vs. 2.89 ± 0.799, respectively). Nevertheless, both had relatively good cell viability (≥50%). Conclusion: Biphasic calcium phosphate obtained from A. granosa shell synthesis exhibits varying ratios; nevertheless, both variants possess favorable attributes for consideration as potential bone substitute materials, as they meet the necessary biocompatibility requirements.
Management of Osteoma Peripheral Type in the Lingual of Right Lower jaw Eddy Hermanto; Andri Hardianto; Winarno Priyanto; Amelia Elizabeth Pranoto
Denta Journal Kedokteran Gigi Vol 17 No 1 (2023): February
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v17i1.5

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Background: Osteoma is benign bone tumors are often found in skeletal bones. These tumors can involve any bone, but usually involve the long bones. The affected bone is generally prominent and clinically visible. This tumor is treated with surgery to remove the tumor mass. The cause of osteoma is unclear, but generally accepted theories are related to embryology, trauma, or infection. Objective: This case reports a osteoma peripheral type in the lingual of right lower jaw and and discuss according to the literature and several other similar cases. Case Report: A 37 years old man with chief complaint swelling in the posterior region right lower jaw for 16 years without pain, fever or any symptom. Case management: Panoramic radiograph and provisional diagnosis was made before surgery. Surgical excision was previously conducted under general anesthesia. Six months after surgery, the patient is in good health. Conclusion: Peripheral osteoma at the lingual surface of the right lower jaw be can be surgically treated well and do not show recurrence.
The effect of adrenaline concentration in lidocaine on prehypertension patients during tooth extraction: an observational analysis Hermanto, Eddy; Arjanti, Hanifah; Laihad, Fanny M.; Pranoto, Amelia Elizabeth; Sari, Rima Parwati; Krisna, Indra Hadi
Padjadjaran Journal of Dentistry Vol 38, No 1 (2026): March 2026
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol38no1.65838

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Introduction: Prehypertension refers to a systolic value 120–139 mmHg or diastolic value 80–90 mmHg.  Studies have shown that the prevalence of prehypertension is over 30% among young adults. The extraction procedure was performed using lidocaine and adrenaline as anesthetics. The purpose of this study was to analyze the effect of adrenaline concentration in lidocaine on prehypertension patients during tooth extraction. Methods: This is a prospective analytical observational study with a pre–post measurement design.  This study was conducted on 36 prehypertensive patients who were divided into 3 groups. Blood pressure was measured before and less than 5 minutes after administration of lidocaine 2% local anesthesia with either 1:80,000, 1:100,000 or 1:160,000 adrenaline, using a calibrated digital tensimeter (TensiOne 1A). Data were analyzed using paired t-tests. Results: Homogeneity test: systole before 0,033, systole after 0,329, diastole before 0,174, diastole after 0,274. Anova test: systole before 0,685, systole after 0,837, diastole before 0,03, diastole after 0,058. Post hoc results showed there were statistically significant differences in diastolic blood pressure in the 1:100.00 and 1:160.000 group before diastole. Before performing the one-way ANOVA and paired t-test, the data were assessed for normality using the Shapiro–Wilk test, which indicated a normal distribution (p > 0.05). Statistical analysis was then conducted using paired t-tests and one-way ANOVA, followed by post hoc testing. Conclusion: The administration of 2% lidocaine with adrenaline concentrations of 1:80,000, 1:100,000, and 1:160,000 did not produce meaningful changes in blood pressure in prehypertensive patients undergoing closed-method dental extraction
Differences in the Accuracy of Age Estimation in Children Using the London Atlas and the Atlas of Dental Development in the Indonesian Population at RSGMP Nala Husada Agitha, Shintya Rizki Ayu; Annariswati, Icha Artyas; Hermanto, Eddy
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 22 No. 1 (2026): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v22i1.12846

Abstract

Introduction: Teeth can be used for age estimation of the victim. Several atlases can be used to estimate age through teeth, such as the London Atlas(AL) and The Atlas of Dental Development in the Indonesian Population(ADDIP). The Atlas of Dental Development in the Indonesian Population (ADDIP) is a modification of the London atlas (AL), made by looking at the process of dental development of children in Indonesia from the age of 5 years to 23 years. Therefore, this study aims to compare the accuracy of child age estimation using the London Atlas (AL) and the Atlas of Dental Development in the Indonesian Population (ADDIP). Material and Methods: This study used 128 panoramic radiographs from pediatric patients aged 5 years to 16 years at RSGMP Nala Husada Surabaya from February to May 2025. Data analysis was conducted using SPSS software for different tests, including a Wilcoxon test. Results and Discussions: Using the London Atlas (AL) method, the average difference between chronological age and dental age was found to be between -0.13 and -1.51 years. Meanwhile, according to ADDIP, the average difference between chronological age and dental age was found to be between -0.03 and -1.31 years. Conclusion: ADDIP shows a smaller difference between chronological age and dental age than the AL, suggesting that ADDIP demonstrated higher accuracy than AL for estimating age in Indonesian children.