Ari Subiyanto
Department Of Conservative Dentistry, Faculty Of Dental Medicine, Universitas Airlangga, Surabaya

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Journal : Conservative Dentistry Journal

Perbedaan daya pembersih kavitas saponin ekstrak kulit manggis (Garcinia mangostana Linn) 0,78% dan asam sitrat 6% (The difference of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity) Ivon Dewi Setianingrum; Ketut Suardita; Ari Subiyanto; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.54 KB) | DOI: 10.20473/cdj.v7i1.2017.6-11

Abstract

Background: Cleanliness of cavity is considered important for a restoration. Smear layer formed after cavity preparation should be removed in order not to disrupt the bond adhesion between restorative materials and dental cavities. Saponins contained in mangosteen pericarp (Garcinia mangostana L.) have surfactant properties that can eliminate the smear layer assessed. 6% citric acid is a chelating agent which can eliminate the inorganic particles of the smear layer. Until now, the research on the differences of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity has never been done. Purpose: To see the differences between 0,78% saponin from mangosteen pericarp extract and 6% citric acid as cavity cleanser. Method: Eighteen human teeth with complete crown, no caries,  and no fractures were randomized in 3 groups (n≥6), in this experiment use (n=6). The cavity was prepared using wheels bur for hand use instrument. After instrumentation, each cavity on the first group used  0,78% saponin from mangosteen pericarp extract as cavity cleanser, the second group used 6% citric acid as cavity cleanser, and the control group used aquadest. Then, the teeth were split to be observed on Scanning Electron Microscope (SEM). Result: For Mann- Whitney test there were significant differences just between 078% saponin from mangosteen pericarp extract with 6% citric acid, and 6% citric acid with aquadest, but not for 0,78% saponin from mangosteen pericarp extract with aquadest. Median value of 6% citric acid showed 2,000 which is the smallest value compared to the value of the other groups. Conclusion: The cleanliness of cavity with 6% citric acid is better than that with 0,78%  saponin from mangosteen pericarp extract. 
Daya bunuh dan daya hambat antimikrobial chlorhexidine 2% dan povidone iodine 1% sebagai medikamen saluran akar terhadap Enterococcus faecalis The ability of chlorhexidine 2% and povidone iodine 1% as root canal medicaments to kill and inhibit Enterococcus faecalis Nathania Astria; Ari Subiyanto; Latief Mooduto
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.683 KB) | DOI: 10.20473/cdj.v7i1.2017.12-17

Abstract

Background. Enterococcus faecalis is one of the pathogenic organisms associated with root canal treatment failure and apical periodontitis. Root canal medicament  is needed to prevent re-infection in the root canal and increase the success of treatment. Chlorhexidine and povidone iodine is a broad spectrum root canal medicaments that can kill gram-positive bacteria. Purpose. The purpose of this study was to discover the ability to kill and inhibit of antimicrobial chlorhexidine 2% and 1% povidone iodine asvroot canal medicaments against bacteria Enterococcus faecalis. Methode. This research was done by measuring the inhibition zone and count the number of colonies. Determination of the inhibition of root canal medicaments against Enterococcus faecalis by diffusion method. 10 microliter root canal medicaments dropped on paperdisk and placed on nutrient agar media with enterococcus faecalis, then inhibition zone was calculated. Determination ability to kill enterococcus faecalis is done by inserting 1 ml medicaments root canal into 5 ml BHIB media, then 0.05 ml inoculum of Enterococcus faecalis inserted into each tube, except the negative control. 0.1 ml of each tube implanted in the media nutrient agar. Media incubated for 24 hours, then Enterococcus faecalis bacterial colonies that grow in media calculated using the CFU. Results. There no colony growth of enterococcus faecalis in both root canal medicaments. There are significant differences in inhibition zone of 2% chlorhexidine and 1% povidone iodine (p<0.05). Conclusion. Both of root canal medicaments can kill enterococcus faecalis, but chlorhexidine 2% was more capable inhibit Enterococcus faecalis.
Kemampuan Bioaktif Glass (Novamin) dan Casein Peptide Amorphous Calcium Phosphate (CPP-ACP) terhadap Demineralisasi Enamel Jeanny Kathleen H; Cecilia G.J. Lunardhi; Ari Subiyanto
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.255 KB) | DOI: 10.20473/cdj.v7i2.2017.111-119

Abstract

Background: Oral cavity is always associated with a dynamic atmosphere where the process of demineralization and remineralization will continue to occur. Caries is a pathological state of continuous demineralization process. Prevention of the occurrence of demineralization process can be done by enhancing remineralization, materials that can be used materials that can trigger a process of remineralization include fluoride, cpp-acp, and novamin. Purpose: The aim of this study was to evaluate and compare the potential of bioactive-Glass (Novamin) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) containing dentifrice on enamel demineralization. Method: A total of 24 sound human premolars were divided into 4 groups and continued into pH cycling regime to be evaluated with Scanning Electron Microscope – Energy Dispersive X-Ray. Result: Group D showed significantly higher values (P < 0.05) when compared with less demineralization lesion than Group A, B, and C. Conclusions: The ability of Novamin on demineralization lesions better than CPP-ACP.
EKSPRESI Nuclear Factor of Activated T cells c-1 (NFATc-1) DAN OSTEOKALSIN PADA KERUSAKAN TULANG PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis Arindah Hadi; M. Roelianto; Ari Subiyanto; Tamara Yuanita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.617 KB) | DOI: 10.20473/cdj.v7i2.2017.138-144

Abstract

Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation then lead to bone destruction. Bone destruction occurs due to the inflammation process that is mediated by immune system. The important cell in the process of bone destruction is osteoclast. Bone destruction is marked by the form of osteoclast that is called osteoclastogenesis. NFATc-1 and osteocalcin play important things in osteoclastogenesis. Purpose. The aim of this study is to know about the expression of NFATc-1 and osteocalcin during the periapical bone destruction due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of periapical bone, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of NFATc-1 and osteoclast increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of NFATc-1 and osteocalcin are increasing during the periapical bone destruction that induced by E.faecalis.
Ekspresi MMP-1 dan TGF-β1 setelah aplikasi dentin bonding berbasis HEMA Adioro Soetojo; Mulia Rahmah; Ari Subiyanto; Moch. Mudjiono
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.424 KB) | DOI: 10.20473/cdj.v8i2.2018.77-84

Abstract

Background: Dentin bonding adalah bahan untuk melekatkan resin komposit terhadap dentin. Komponen utama penyusun dentin bonding adalah 2-hydroxyethyl methacrilate (HEMA). Setelah polimerisasi, terdapat monomer sisa yang akan berdifusi ke dalam tubuli dentin sampai ke dalam pulpa yang dapat mempengaruhi sel odontoblas. Objective: Menganalisis ekspresi MMP-1 dan TGF-β1 pada dentin gigi tikus yang diberikan dentin bonding HEMA dan HEMA murni. Methods: Gigi molar pertama rahang atas tikus wistar dipreparasi klas 1 tanpa aplikasi bahan selama 3 hari (kelompok 1), kemudian diaplikasikan HEMA murni selama 3 hari (kelompok 2), dentin bonding HEMA selama 3 hari (kelompok 3). Kemudian gigi ditumpat dengan Fuji II LC. Tikus wistar di dekaputasi dan rahangnya direseksi pada hari ke 3 kemudian diproses untuk pemeriksaan histokimia. Result: Ekspresi MMP-1 pada penggunaan dentin bonding HEMA lebih sedikit dibandingkan dengan HEMA murni,  ekspresi  TGF-β1 pada penggunaan dentin bonding lebih banyak dibandingkan dengan HEMA murni. Conclusion : Ada perbedaan bermakna pada ekspresi MMP-1 dan TGF-β1 antara penggunaan dentin bonding HEMA dibandingkan dengan HEMA murni
Perbedaan ketebalan odontoblast-like cells setelah aplikasi CAPE dan Kalsium Hidroksida Putri Galuh Prawitasari; Karlina Samadi; Ari Subiyanto
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.899 KB) | DOI: 10.20473/cdj.v8i2.2018.118-122

Abstract

Background : CAPE is the main component of propolis, it has several biology and fpharmocological advantages as antioxidant, antiinflammation, anticancer and as an immunomodulator. There were the research before of CAPE is capable to stimulate the production of the TGF-β 1 and collagen sintesis by the pulp tissue with many superiority compare to Ca(OH)2, recently Ca(OH)2 is  the gold standard for pulp capping treatment procedure. Objective : To determine and compare the effectiveness of CAPE and Ca(OH)2 to the thickness of odontoblast-like cells in rat pulp tissue. Methods : Maxillary first molar tooth of wistar mice was class 1 preparated until the pulp opened, then Ca(OH)2 was aplicated for 14 and 28 days, CAPE for 14 and 28 days. After application of Ca(OH)2 and propolis extract, the tooth was filled with RMGIC. Teeth were extracted on defined day and processed for histological evaluation. Result : There is a significant difference in the thickness of odontoblast-like cells after application of CAPE for 14 days with Ca(OH)2 for 14 days and CAPE for 28 days with Ca(OH)2 for 28 days. Conclusion : odontoblast-like cells after aplication of CAPE extract material is thicker than Ca(OH)2.
Endodontic Retreatment In Case Of Failure: Case Report Kevin Prayogo; Dian Agustin Wahjuningrum; Ari Subiyanto
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.606 KB) | DOI: 10.20473/cdj.v9i2.2019.109-111

Abstract

Background: In medical practice, clinicians come across a lot of obstacle during a treatment , that can lead to a failure, like other dental treatments, endodontics too can fail. Inappropriate mechanical debridement, persistence of bacteria in the canals and apex, poor obturation quality, over and under extension of the root canal filling, and coronal leakage are some of the commonly attributable causes of failure. Despite the high success rate of endodontic treatment, failures do occur in a large number of cases and most of the times can be attributed to the already stated causes. Purpose: Endodontic retreatment of a failure is required by the increased desire to preserve the tooth on the dental arch, preventing the need for dental extraction that may have adverse consequences in terms of functional and psychological effect on patients. Case: This article presents a case report about dental retreatment with 2 common failure, that was under filling obturation and non-fit post. Case management: The failure was corrected with endodontic retreatment, and finally restored with fiber post and porcelain fused to metal crown. Conclusion: Endodontic retreatment was done successfully on underfilling obturation and non-fit post. The patient was satisfied with the result.
Comparison of ratios combination calcium hydroxide Ca(OH)2 with red pine (Pinus densiflora) of viscosity Latief Mooduto; Ari Subiyanto; Shafa Prasita
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.50-55

Abstract

Background: Calcium hydroxide is a gold standard for root canal sealing material. However, there are many defects in calcium hydroxide that have prompted many researchers to look for alternative materials, including natural origin ingredients. Red pine (Pinus densiflora) is often used in dentistry for its antioxidant and antibacterial properties. The mixture of calcium hydroxide and red pine has never been achieved before. One of the criteria for material sealing is that the channel should be fully sealed with high flow ability and low viscosity. Therefore, this research is performed to analyze the viscosity value of the mixture of calcium hydroxide and red pine (Pinus densiflora). Purpose: Knowing the difference in viscosity of the combination of calcium hydroxide and red pine with a ratio of 1:1, 1:1.5, and 1:2. Methods: This study used four treatment groups, with each group consisting of 8 replications. Group 1 was a combination of calcium hydroxide and red pine with a 1: 1, group 2 the ratio was 1: 1.5, group 3 had the ratio 1: 2, and the positive control group used calcium hydroxide and a sterile aquadest. Calcium hydroxide in powder form and an extract of red pine in liquid form are mixed according to the ratio. The viscosity value is measured using a viscosity tester, namely the Brookfield Viscometer. Results: The control group had a lower viscosity than group 3, group 3, and group 2 than group 1. Conclusion: Combination of calcium hydroxide and red pine with a ratio of 1:2, the lowest viscosity was obtained compared to the ratio of 1: 1 and 1: 1.5