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Jayus Jayus

*Centre For Development Of Advance Science And Technology, And Agricultural Product Technology Department. The University Of Jember

Author-ID : 3234918

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Law, Crime, Criminology & Criminal Justice Social Sciences Other

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DEGRADASI KOMPONEN SELULOSA, HEMISELULOSA, DAN PATI TEPUNG KULIT UBI KAYU MENJADI GULA REDUKSI OLEH Aspergillus niger, Trichoderma viride, DAN Acremonium sp. IMI 383068 Jay Jayus; Ahmad Nafi'; Anis Shabrina Hanifa
JURNAL AGROTEKNOLOGI Vol 13 No 01 (2019)
Publisher : Faculty of Agricultural Technology, University of Jember

As the solid waste produced from cassava processing industry such as tapioca factory or its derivatives, the cassava peel is potential to be use as a source of reducing sugar through hydrolysis process, since the peels contains a high amount of starch and lignocellulose components. The more environmentally friendly enzymatic hydrolysis using several microorganisms will be introduced in this study as an alternative to avoid the unsafe acid hydrolysis. However, the hydrolysis process using a single microorganism is not efficient since the hydrolytic enzyme produced is limited to a single enzyme, while the component in the cassava peels to be hydrolyzed is diverse which include cellulose, lignin, hemicellulose and starch. Therefore, it is necessary to optimize the hydrolysis process by combining several microorganisms (A. niger, T. viride and Acremonium sp. IMI 383068) which produced different specificity of hydrolytic enzyme depending on the substrate available in the cassava peels. The aims of this research were to determine the effect of single and mixed culture on the amount of reducing sugar released during the simultaneous cultivation. The result showed that the use of simultaneous mixed cultures during hydrolysis process was able to produce higher reducing sugar compare to that of single culture. The hydrolysis of cassava flour using a single strain of A. niger, T. viride and Acremonium sp. IMI 383068 respectively produced 4.86 g/L, 4.02 g/L, and 1.68 g/L of reducing sugar, while the hydrolysis of it using simultaneous mixed cultures of A. niger, T. viride, and Acremonium sp. IMI 383068 produced 7.23 g/L of reducing sugar. Keywords: cassava peels, hydrolysis, reducing sugar

Pelarutan P dan K dari batuan leusit dan apatit menggunakan kombinasi senyawa humat-BPF-BPK Mohammad Jimmy KURNIATA; Tri Candra SETIAWATI; Jay JAYUS
E-Journal Menara Perkebunan Vol 87, No 2 (2019): OKTOBER, 2019
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractDue to the limited availability of potassium and phosphate in soil and the high price of their synthetic mineral fertilizers, the importance of minerals-solubilizing microorganismshas been increasingly significant aimed at sustainable agriculture achievement. Apatite and leucite are considerably abundant minerals; however, their solubility is low. This study was aimed to study the effects of the combination of phosphate-and potassium-solubilizing bacteria (PhSB and PSB) and humic acid of cassava as bioleaching agents in the solubilization process of potassium and phosphate from agromineral material. Some leucite agromineral materials were obtained from Situbondo and Pati, while apatite materials were obtained from Tuban and Ciamis, Indonesia. The minerals were treated with 2.10 x 107CFU/g PhSB and 1.61 x 107CFU/g PSB isolates, combined with 100 ppm organic C humic compounds from cassava as the media. The minerals solubility was monitored every two weeks for 12 weeks ofobservation, which includes the concentration of dissolved K and P, as well as the pH of the media. The presence of organic acids was observed to monitor the bacterial activity by using HPLC, while the physical changes of the rock surface due to bacterial dissolution were scanned by using scanning electron microscopy (SEM). The results showed that the highest dissolution of P was recorded at week 4 (344.23 ppm) released from Tuban apatite with the combination of PhSB and PSB under the humic compound.In contrast, the highest K dissolution was obtained at week 6 for Situbondo leucite (44.21 me/100 g) with a combination of humic cassava compound and PSB only. Statistical analyses showed a mark different on both minerals for dissolution of K and P. The SEM result indicates breakage of the rock surface after a three month observation period indicating that the dissolution minerals occurred. Selected organic acids such as citric, ferulic, coumaric, syringic, and malic acids were detected during the treatment.[Keywords: leucite, apatite, humic compounds, organic acid] Abstrak Ketersediaan kalium (K) dan fosfat (P) dalam tanah terbatas,danharga pupuk sintetik relatif mahal, menjadikan mikroorganismepelarut mineral memegang peranan penting dalam menunjang pertanian berkelanjutan.Batuan mineral apatit dan leusitmerupakan sumber daya mineral dengan ketersediaan tinggi tetapi mempunyai kelarutan mineral rendah. Penelitian bertujuan untukmempelajari pengaruh kombinasi bakteri pelarut fosfat (BPF), bakteri pelarut kalium (BPK), dan humatsingkong sebagai agen bioleachingdalam proses pelarutan K dan P dari bahan agromineral. Bahan agromineral leusitdiperoleh dariKabupatenSitubondo dan Pati, sedangkan bahan apatit berasal dari Kabupaten Tuban dan Ciamis, Indonesia. Bahan mineral diperlakukan dengan 2,10 x107CFU/gBPF dan 1,61 x 107CFU/gBPK, dikombinasikan dengan 100 ppm C organik senyawa humatdari singkong sebagai media. Kelarutan mineral diamati setiap dua minggu sekali selama 12 minggu meliputi kelarutan K dan P, dan pH media. Produksi asam organik dianalisis untuk mengamati aktivitas bakteri menggunakan HPLC dan perubahan fisik permukaan batuan akibat pelarutan bakteri dipindai menggunakan SEM.Hasil penelitian menunjukkan bahwa pelarutan fosfat tertinggi tercatat pada minggu ke 4 (344,23 ppm) yang dilepaskan dari apatit Tuban dengan kombinasi BPF dan BPK dengan senyawa humat, sedangkan pelarutan kalium tertinggi diperoleh pada minggu ke 6 dari leusitSitubondo (44,21 me / 100 g) dengan kombinasi senyawa humatsingkong dan BPK. Secara statistikpada kedua mineral pelarutan K dan P menunjukkan berbedanyata. Hasil analisis dengan menggunakan SEMterjadi kerusakan permukaan batuansetelah periode pengamatan tigabulan yang menunjukkan bahwa terjadi pelarutan mineral. Selama percobaan dideteksi beberapa asam organik seperti asam sitrat, ferulat, kumarat, siringatdan malat. [Kata kunci: leusit, apatit,senyawa humat,asam organik]

Pelarutan P dan K dari batuan leusit dan apatit menggunakan kombinasi senyawa humat-BPF-BPK Mohammad Jimmy KURNIATA; Tri Candra SETIAWATI; Jay JAYUS
Menara Perkebunan Vol. 87 No. 2 (2019): 87 (2), 2019
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v87i2.330

AbstractDue to the limited availability of potassium and phosphate in soil and the high price of their synthetic mineral fertilizers, the importance of minerals-solubilizing microorganismshas been increasingly significant aimed at sustainable agriculture achievement. Apatite and leucite are considerably abundant minerals; however, their solubility is low. This study was aimed to study the effects of the combination of phosphate-and potassium-solubilizing bacteria (PhSB and PSB) and humic acid of cassava as bioleaching agents in the solubilization process of potassium and phosphate from agromineral material. Some leucite agromineral materials were obtained from Situbondo and Pati, while apatite materials were obtained from Tuban and Ciamis, Indonesia. The minerals were treated with 2.10 x 107CFU/g PhSB and 1.61 x 107CFU/g PSB isolates, combined with 100 ppm organic C humic compounds from cassava as the media. The minerals solubility was monitored every two weeks for 12 weeks ofobservation, which includes the concentration of dissolved K and P, as well as the pH of the media. The presence of organic acids was observed to monitor the bacterial activity by using HPLC, while the physical changes of the rock surface due to bacterial dissolution were scanned by using scanning electron microscopy (SEM). The results showed that the highest dissolution of P was recorded at week 4 (344.23 ppm) released from Tuban apatite with the combination of PhSB and PSB under the humic compound.In contrast, the highest K dissolution was obtained at week 6 for Situbondo leucite (44.21 me/100 g) with a combination of humic cassava compound and PSB only. Statistical analyses showed a mark different on both minerals for dissolution of K and P. The SEM result indicates breakage of the rock surface after a three month observation period indicating that the dissolution minerals occurred. Selected organic acids such as citric, ferulic, coumaric, syringic, and malic acids were detected during the treatment.[Keywords: leucite, apatite, humic compounds, organic acid] Abstrak Ketersediaan kalium (K) dan fosfat (P) dalam tanah terbatas,danharga pupuk sintetik relatif mahal, menjadikan mikroorganismepelarut mineral memegang peranan penting dalam menunjang pertanian berkelanjutan.Batuan mineral apatit dan leusitmerupakan sumber daya mineral dengan ketersediaan tinggi tetapi mempunyai kelarutan mineral rendah. Penelitian bertujuan untukmempelajari pengaruh kombinasi bakteri pelarut fosfat (BPF), bakteri pelarut kalium (BPK), dan humatsingkong sebagai agen bioleachingdalam proses pelarutan K dan P dari bahan agromineral. Bahan agromineral leusitdiperoleh dariKabupatenSitubondo dan Pati, sedangkan bahan apatit berasal dari Kabupaten Tuban dan Ciamis, Indonesia. Bahan mineral diperlakukan dengan 2,10 x107CFU/gBPF dan 1,61 x 107CFU/gBPK, dikombinasikan dengan 100 ppm C organik senyawa humatdari singkong sebagai media. Kelarutan mineral diamati setiap dua minggu sekali selama 12 minggu meliputi kelarutan K dan P, dan pH media. Produksi asam organik dianalisis untuk mengamati aktivitas bakteri menggunakan HPLC dan perubahan fisik permukaan batuan akibat pelarutan bakteri dipindai menggunakan SEM.Hasil penelitian menunjukkan bahwa pelarutan fosfat tertinggi tercatat pada minggu ke 4 (344,23 ppm) yang dilepaskan dari apatit Tuban dengan kombinasi BPF dan BPK dengan senyawa humat, sedangkan pelarutan kalium tertinggi diperoleh pada minggu ke 6 dari leusitSitubondo (44,21 me / 100 g) dengan kombinasi senyawa humatsingkong dan BPK. Secara statistikpada kedua mineral pelarutan K dan P menunjukkan berbedanyata. Hasil analisis dengan menggunakan SEMterjadi kerusakan permukaan batuansetelah periode pengamatan tigabulan yang menunjukkan bahwa terjadi pelarutan mineral. Selama percobaan dideteksi beberapa asam organik seperti asam sitrat, ferulat, kumarat, siringatdan malat. [Kata kunci: leusit, apatit,senyawa humat,asam organik]

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