Bremmy Laksono
Departemen Oral Biologi, Fakultas Kedokteran Gigi, Universitas Padjadjaran, Bandung, Indonesia, 40132

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Perbedaan potensi antibakteri ekstrak metanol umbi sarang semut (Myrmecodia pendens Merr. & Perry) dan NaOCl terhadap Streptococcus mutans (ATCC 25175)The antibacterial potential differences between the methanolic extract of ant-plant (Myrmecodia pendens Merr. & Perry) tubers and NaOCl towards Streptococcus mutans (ATCC 25175) Apriyanti, Eria Ariningtyas; Satari, Mieke Hemiawati; Laksono, Bremmy
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 28, No 2 (2016): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.406 KB) | DOI: 10.24198/jkg.v28i2.18704

Abstract

Pendahuluan: Streptococcus mutans merupakan organisme kariogenik utama. Antibakteri sintetik yang biasa digunakan adalah NaOCl. Sarang semut mengandung senyawa kimia yang dapat menghambat pertumbuhan Streptococcus mutans. Tujuan penelitian ini adalah mengetahui daya hambat ekstrak metanol sarang semut (Myrmecodia pendans Merr. & Perry) dan NaOCl terhadap Streptococcus mutans serta perbedaan potensi antibakteri keduanya. Metode: Jenis penelitian adalah eksperimental laboratoris. Uji daya antibakteri dilakukan dengan menggunakan metode difusi agar. Media agar darah ditetesi 0,1 ml suspensi bakteri lalu dimasukkan ekstrak metanol sarang semut dengan konsentrasi 30%, 15%, 7,5%, 3,75%, 1,875% dan NaOCl dengan konsentrasi 5%, 2,5%, 1,25% diinkubasi pada suhu 37° C selama 24 jam dalam suasana fakultatif anaerob dan dilakukan replikasi tiga kali. Uji statistik menggunakan metode ANAVA dan Independent t-test. Hasil: Ekstrak metanol sarang semut konsentrasi 30% menghasilkan diameter zona hambat rata-rata 5,87 mm, sedangkan NaOCl 1,25% adalah 9,33 mm. Simpulan: Ekstrak metanol sarang semut dan NaOCl memiliki potensi daya hambat terhadap Streptococcus mutans, namun potensi antibakteri NaOCl lebih besar dibandingkan dengan ekstrak metanol sarang semut. ABSTRACTIntroduction: Streptococcus mutans is the main cariogenic organism. The synthetic antibacterial commonly used is NaOCl. Ant nests contain chemical compounds that can inhibit the growth of Streptococcus mutans. The purpose of this study was to determine the inhibitory power of methanol extracts of ant nests (Myrmecodia pendans Merr. & Perry) and NaOCl against Streptococcus mutans and the differences in antibacterial potential both. MethodS: This type of research is experimental laboratory. Antibacterial power test was carried out using agar diffusion method. Blood agar media was dripped with 0.1 ml of bacterial suspension and then inserted methanol extract of ant nests with a concentration of 30%, 15%, 7.5%, 3.75%, 1.875% and NaOCl with a concentration of 5%, 2.5%, 1, 25% was incubated at 37C for 24 hours in a facultative anaerobic atmosphere and replicated three times. Statistical test using ANAVA method and Independent t-test. Results: Methanol extract of ant nest 30% resulted in an average inhibition zone diameter of 5.87 mm, while 1.25% NaOCl was 9.33 mm. Conclusion: Methanol extract of ant nests and NaOCl has potential inhibitory power against Streptococcus mutans, but the antibacterial potency of NaOCl is greater than that of methanol extracts of ant-plants.Keywords: Antibacterial, methanolic extract, ant-plants, NaOCl, Streptococcus mutans.
Sindrom Patau dengan genotipe 47,xy,+13,t(13:18) Vanda Elfira; Fiva Aprilia Kadi; Bremmy Laksono; Sjarif Hidajat Effendi
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n3.1389

Abstract

Trisomy 13 (Patau syndrome) is cytogenetically classified as a 47,XY,+13 or 47,XX,+13, due to nondisjunction at meiosis I or II, or at mitosis (mosaicism), and partial trisomy due to translocation. Patau syndrome is one of the most common chromosomal anomalies with an estimated incidence of about 1/10,000 births characterized by the presence of cleft lip and/or palate, post axial polydactyly, low set ears, rocker-bottom feet, cryptorchidism, and congenital heart disease. This was a case report of a newborn baby in Dr. Hasan Sadikin General Hospital Bandung in January 2016 with translocation of chromosome 13 segment to chromosome 18 or 47,XY,+13,t(13:18). Sindrom Patau dengan Genotype 47,XY,+13,t(13:18)Trisomi 13 (sindrom Patau) secara sitogenetik diklasifikasikan 47,XY,+13 atau 47,XX,+13, disebabkan oleh kegagalan pemisahan pada meiosis I atau II, atau pada mitosis (mosaicism) dan trisomi parsial yang disebabkan oleh translokasi. Sindrom Patau merupakan salah satu anomali kromosom yang paling sering dengan insidensi sekitar 1/10.000 kelahiran, dengan karakteristik fenotipe seperti celah bibir dan atau celah langit-langit, postaxial polydactyly, low set ears, rocker bottom feet, kriptokismus, serta kelainan jantung kongenital. Dilaporkan kasus sindrom Patau pada bayi baru lahir di Rumah Sakit Dr. Hasan Sadikin Bandung pada Januari 2016 yang menunjukkan segmen dari kromosom 13 translokasi ke kromosom 18 atau 47,XY,+13,t(13:18).
Efek perendaman pada landasan akrilik self-cured terhadap viabilitas sel fibroblasImmersion effect on self-cured acrylic base towards the fibroblast cell viability Laurensius Randy Soetono; Taufik Sumarsongko; Lisda Damayanti; Bremmy Laksono
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 32, No 1 (2020): April 2020
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v32i1.18075

Abstract

Pendahuluan: Akrilik merupakan salah satu bahan landasan gigi tiruan yang populer digunakan oleh dokter gigi di bidang prostodonti. Pembuatan gigi tiruan saat ini dapat dilakukan juga oleh tukang gigi sesuai dengan peraturan Permenkes, namun terbatas pada bahan akrilik heat-cured sebagai landasan gigi tiruan. Tukang gigi diketahui sering menggunakan bahan akrilik self-cured sebagai landasan gigi tiruan, tanpa memperhatikan toksisitas dari bahan. Penelitian ini bertujuan untuk mengamati pengaruh toksisitas akrilik self-cured terhadap viabilitas sel fibroblast. Metode: Penelitian menggunakan metode eksperimental in vitro dengan melakukan kontak antara piringan akrilik self-cured dan sel fibroblast REF (Rat Embryonic Fibroblast) selama 48 jam. Piringan akrilik self-cured yang digunakan pada penelitian ini dibuat dengan ukuran diameter 1 cm dan tebal 1 mm. Pengaruh toksisitas piringan akrilik self-cured dilihat dengan membandingkan viabilitas sel fibroblast pada piringan akrilik self-cured yang sudah direndam pada air suhu ruangan (25o C) selama 1 jam dengan monomer sisa yang terukur sebagai kontrol (2,5%). Hasil: Jumlah rata-rata viabilitas sel fibroblast pada piringan akrilik self-cured sebesar 24,04% dan pada kontrol 17,43%. Analisis statistik uji-t menunjukkan nilai p dibawah nilai kemaknaan (p < 0,05), yaitu 0,02. Simpulan: Terdapat pengaruh toksisitas bahan landasan akrilik self-cured terhadap viabilitas sel fibroblast dan adanya perbedaan bermakna pada perendaman piringan akrilik dalam air suhu ruangan selama 1 jam terhadap kontrol.Kata kunci: Akrilik self-cured, sel fibroblas, viabilitas sel. ABSTRACTIntroduction: Acrylic is one of denture base’s materials which are popularly used by dentists in prosthodontic. Denture manufacture can be done by artisans corresponding to National Health Regulation (Permenkes) but is limited on heat-cured acrylic material only. Dental artisans are usually known to use self-cured acrylic as denture base material, without paying attention to its toxicity. This study was aimed to evaluate the toxicity effect of self-cured acrylic by measuring the viability of fibroblast cells in-vitro. Methods: This research was an in-vitro experimental by contacted self-cured acrylic discs with REF (Rat Embryonic Fibroblast) for 48 hours. Self-cured acrylic discs that used in this research were made in disc shape with 1 cm in diameter and 1 mm thick. Toxicity effect of self-cured acrylic discs was measured by comparing cell viability at self-cured acrylic discs that had been immersed in water with room temperature (25oC) for 1 hour to measured residual monomer as control (2.5%). Results: The research result was showing the average cells viability at self-cured discs was 24.04%, and the control group was 17.43%. The t-test analysis result showed that the p-value was p = 0.02. Conclusion: There was a toxicity effect of acrylic self-cured materials towards the fibroblast cell viability. This research also found a difference between the acrylic self-cured discs that had been immersed in room temperature water for 1 hour with the control group.Keywords: Cell viability, fibroblast cells, self-cured acrylic.
Perbedaan potensi antibakteri ekstrak metanol umbi sarang semut (Myrmecodia pendens Merr. & Perry) dan NaOCl terhadap Streptococcus mutans (ATCC 25175)The antibacterial potential differences between the methanolic extract of ant-plant (Myrmecodia pendens Merr. & Perry) tubers and NaOCl towards Streptococcus mutans (ATCC 25175) Eria Ariningtyas Apriyanti; Mieke Hemiawati Satari; Bremmy Laksono
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 28, No 2 (2016): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.406 KB) | DOI: 10.24198/jkg.v28i2.18704

Abstract

Pendahuluan: Streptococcus mutans merupakan organisme kariogenik utama. Antibakteri sintetik yang biasa digunakan adalah NaOCl. Sarang semut mengandung senyawa kimia yang dapat menghambat pertumbuhan Streptococcus mutans. Tujuan penelitian ini adalah mengetahui daya hambat ekstrak metanol sarang semut (Myrmecodia pendans Merr. & Perry) dan NaOCl terhadap Streptococcus mutans serta perbedaan potensi antibakteri keduanya. Metode: Jenis penelitian adalah eksperimental laboratoris. Uji daya antibakteri dilakukan dengan menggunakan metode difusi agar. Media agar darah ditetesi 0,1 ml suspensi bakteri lalu dimasukkan ekstrak metanol sarang semut dengan konsentrasi 30%, 15%, 7,5%, 3,75%, 1,875% dan NaOCl dengan konsentrasi 5%, 2,5%, 1,25% diinkubasi pada suhu 37° C selama 24 jam dalam suasana fakultatif anaerob dan dilakukan replikasi tiga kali. Uji statistik menggunakan metode ANAVA dan Independent t-test. Hasil: Ekstrak metanol sarang semut konsentrasi 30% menghasilkan diameter zona hambat rata-rata 5,87 mm, sedangkan NaOCl 1,25% adalah 9,33 mm. Simpulan: Ekstrak metanol sarang semut dan NaOCl memiliki potensi daya hambat terhadap Streptococcus mutans, namun potensi antibakteri NaOCl lebih besar dibandingkan dengan ekstrak metanol sarang semut.Kata kunci: Antibakteri, ekstrak metanol, sarang semut, NaOCl, Streptococcus mutans. ABSTRACTIntroduction: Streptococcus mutans is the main cariogenic organism. The synthetic antibacterial commonly used is NaOCl. Ant nests contain chemical compounds that can inhibit the growth of Streptococcus mutans. The purpose of this study was to determine the inhibitory power of methanol extracts of ant nests (Myrmecodia pendans Merr. & Perry) and NaOCl against Streptococcus mutans and the differences in antibacterial potential both. Methods: This type of research is experimental laboratory. Antibacterial power test was carried out using agar diffusion method. Blood agar media was dripped with 0.1 ml of bacterial suspension and then inserted methanol extract of ant nests with a concentration of 30%, 15%, 7.5%, 3.75%, 1.875% and NaOCl with a concentration of 5%, 2.5%, 1, 25% was incubated at 37°C for 24 hours in a facultative anaerobic atmosphere and replicated three times. Statistical test using ANAVA method and Independent t-test. Result: Methanol extract of ant nest 30% resulted in an average inhibition zone diameter of 5.87 mm, while 1.25% NaOCl was 9.33 mm. Conclusion: Methanol extract of ant nests and NaOCl has potential inhibitory power against Streptococcus mutans, but the antibacterial potency of NaOCl is greater than that of methanol extracts of ant-plants.Keywords: Antibacterial, methanolic extract, ant-plants, NaOCl, Streptococcus mutans.
Frequency of MTHFR GENE C677T Polymorphism for Non-Syndromic Autism Spectrum Disorder Patients Bremmy Laksono; Ani Melani Maskoen; Tri Indah Winarni; Syarief Taufik; Sultana MH Faradz
Journal of Biomedicine and Translational Research Vol 1, No 2 (2015): December 2015
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.554 KB) | DOI: 10.14710/jbtr.v1i2.61

Abstract

Background: The folate metabolism is a pathway that may involve in the non-syndromic Autism Spectrum Disorder (ASD). Methylenetetrahydrofolate reductase enzyme has a key role in folate metabolism. The C677T polymorphism of MTHFR gene could reduce the effectiveness of the enzyme.Objectives: To evaluate the frequency of MTHFR geneC677T polymorphism for non-syndromic ASD patients.Method: Thirty-four DNA samples were taken from each group. PCR mixture was consisted of 1µL DNA, 2.5µL PCR buffer, 0.5µL dNTP, 1.5µL MgCL2, 0.125µLTaqenzyme, 0.5µLofforwardandreverseprimerandaquabidesttoreach a volume of 25 µL. The PCR profiles were initiation 95ºC for 5 min, denaturation 94ºC for 1min, annealing 55ºCfor 45 seconds, and elongation 72ºC for30 seconds. The cycles were done in 35 times an dfinal elongation was at 72ºC for 5min. The PCR product was 198bp, and then digested by the Hinfl enzyme for 16hours at 37°C, and visualized using2%agarosegeland then electrophoresed for 30 minutes at 100 volts.Result: Non-syndromic ASD samples showed none had homozygote mutant type (677TT), 3 (8.8%) samples had heterozygote (677CT)and 31 (91.2%) samples had wild type (677CC). Meanwhile, normal control showed only 1 (2.9%)sample had homozygote mutant type(677TT), 9 (26.5%) samples had heterozygote (677CT)and 24 (70.6%) samples had  wild type (677CC).Conclusion: The frequency of MTHFR geneC677T polymorphism in patients with non-syndromic ASD and controls are not significantly different.
Kelebihan dan kekurangan penggunaan radiasi dosis rendah (Low Doses Radiation: advantages and disadvantages) Lusi Epsilawati; Azhari Azhari; Bremmy Laksono; Haris Nasutianto; Eha Renwi Astuti
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Vol 4 No 3 (2020): Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI)
Publisher : Ikatan Radiologi Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jrdi.v4i3.633

Abstract

Objectives: To inform and discuss the benefits and harms of low dose radiation. Literature Review: Low dose radiation (LDR) was often used, including for services in the field of dentistry. Several studies have studied this for a long time but the results are still not conclusively agreed. Some researchers found a variety of side effects that are not beneficial to the body, but some found no association with the body damage caused by this, and even some found that LDR has beneficial effects on the body. Conclusion: low doses of radiation cause significant changes in the body, although this condition is met with an adaptive reaction which is considered a beneficial response by the body. Therefore, no matter how small the radiation is given, it will still have an impact on the body so it is better to be wise in its use.
The Association between the rs35874116 Gene TAS1R2 Polymorphism with Caries Index Pelangi Mumtazdhia; Ani Melani Maskoen; Bremmy Laksono; Witri Ardini
The Avicenna Medical Journal Vol 3, No 1 (2022)
Publisher : Faculty of Medicine, UIN (State Islamic University) Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/avicenna.v3i1.27319

Abstract

Background: Dental caries is a multifactorial disease influenced by complex genetic and environmental factors. At least 27 gene polymorphisms have been identified that are associated with caries risk. Polymorphisms of genes encoding taste receptors, such as the TAS1R2 gene, can cause individual differences in perception and sensitivity to sweet tastes. Research shows that the TAS1R2 gene polymorphism is associated with the consumption of sweet foods, which affects increasing caries risk. This study aims to determine the relationship between the TAS1R2 gene rs35874116 polymorphism and the caries index in Tangerang Selatan population. Methods: This study is observational with a cross-sectional method involving 266 research subjects (45 males and 221 females; 20-55 years). DNA was extracted from venous blood using the Genomic DNA Mini Kit from Geneaid and the genotyping process using the rhAmp-SNP assay kit, which was analyzed using RT-PCR. Dental caries was assessed by calculating D(decayed), M(missing), and F(filled) on permanent teeth. The hypothesis between the TAS1R2 genotype and the DMF-T index was tested using the Mann-Whitney test, while the comparison between the TAS1R2 genotype and caries risk was tested using Kruskal-Wallis' test. The results of the study were considered significant if p<0.05. Results: The minor allele frequency was 16.5%. The proportion of TT, TC, and CC genotypes were 71.%, 24.1%, and 4.5%, respectively. According to WHO classification, the DMF-T index of all research subjects was 5.62, a high category. The group of subjects with the CC genotype had a higher DMF-T index (7.08; very high) than the group of subjects with the CT genotype (6.05; high) and TT (5.38; high). CT and TT genotypes with high risk were significantly lower than those with low-moderate caries risk. Conclusion: there is no significant association between the TAS1R2 gene polymorphism rs35874116 with the caries index value in the people in South Tangerang.