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Happy Harmono
Bagian Biomedik, Fakultas Kedokteran Gigi, Universitas Jember, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Library & Information Science Medicine & Pharmacology Nursing Public Health

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Daya Hambat Ekstrak Biji Kopi Robusta (Coffea robusta) terhadap Pertumbuhan Fusobacterium nucleatum Hakima, Anisa Nur; Ermawati, Tantin; Harmono, Happy
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 15 No 2 (2018)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v15i2.17932

Abstract

Fusobacterium nucleatum is a bacteria found in normal flora of the oral cavity that plays a role in the occurrence of periodontal disease. The prevalence of periodontal disease in Indonesia reaches 70%. One treatment by using medicinal plants is robusta coffee beans. Robusta coffee beans contain volatile acids, chlorogenic acids, caffeine acids, phenols and caffeine suspected to be antibacterial. The objective of this study was to determine the inhibition of coffee robusta extract on growth of Fusobacterium nucleatum. This study used disc diffusion method with 4 samples in each study group. The study group consisted of 6 treatment groups (12,5%, 25%, 50% and 100% coffee robusta extract), positive control group (chlorhexidine gluconate 0,2%), and negative control group (sterile aquades). Data were analyzed using One Way Anova test and LSD (Least Significant Difference) test. Coffee robusta extract has the ability to inhibit the growth of Fusobacterium nucleatum. The concentration of coffe robusta extract 100%, 50%, 25%, 12,5% have same ability to inhibit the growth of Fusobacterium nucleatum.
Inhibition activity of Robusta coffee beans polyphenol extract on the production of TNF-α neutrophil cells Tantin Ermawati; Zahara Meilawaty; Happy Harmono
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1230.046 KB) | DOI: 10.22146/majkedgiind.28352

Abstract

Polyphenols are one of the active substances in the Robusta coffee beans with various benefits for humans’ health including anti-inflammation. neutrophil cell (polymorphonuclear PMN) plays a significant role as the primary immune response against foreign agent. Inflammatory response is characterized by the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α). Hence, the purpose of this study is to determine anti-inflammation capacity of Robusta coffee beans polyphenols extract on the TNF-α production in neutrophil cells. Neutrophil was derived from human peripheral venous blood by means of gradient density method. One hundred microliter of neutrophil  (1,5x103 cell) was incubated with 3.13%, 6.25%, 12.5%, 25% polyphenol extract and exposed with 100 μl of Lipopolysaccharides (LPS) 25 mg/ml. The production of TNF-α were determined by ELISA. Data were analyzed by one way Anova. Polyphenols of Robusta coffee bean extract was shown to inhibit TNF-α production in LPS-exposed neutrophil cells compared to controls. Whereas, LPS-exposed neutrophil increased TNF-α production. The most effective concentration to inhibit TNF-α production was 12.5%. It was concluded that polyphenols of Robusta coffee beans has anti-inflammatory properties as indicated by its ability to decrease TNF-α levels.
Pengaruh Jus Tomat Segar (Lycopersicon esculentum Mill) terhadapKadar Trigliserida dalam Darah Tikus Wistar Jantan yang Diberi Lipid Peroral (The Effect of Fresh Tomato Juice (Lycopersicon esculentum Mill) to The Triglyceride Levels in The Blood of Male W Riclas Yusuf Punta Wibisono; Happy Harmono; Erna Sulistyani
Pustaka Kesehatan Vol 2 No 3 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Increased levels of lipid which is characterized by increasedlevels of triglycerides until now has not found an effective and efficient. Some sources say there are9-oxo-ODA in tomatoes to cause triglyceride levels in the blood. Purpose : The aim of The Study todetermineeffect of tomatoe juice on blood serum Triglycerides level inmalewistarrat. This experimental study used male wistar rats, and the post test only control group design. Rats were divided into three groups, i.e. negative control group (K-), positif control group (K+), and treatment group (P). K-group was untreated by fresh tomato juice and fed with standart diet only. K+ group was fed with standart and peroral lipid. P group treated with fresh tomato juice and also fed with standart and lipid peroral.Fresh Tomato juice were treated by means of stomach sondation for 14 days. On day the 15th, rats were sacrified and intracardial blood were taken 3-5 ml for examination of lipid profile by enzimatic colorimetric method using an automatic test analizer. Data were analyzed by one way anova followed by LSD test. The conclusion of this study is the average triglyceride levels between the negative control group (K-) and positive control (K +) was not significantly different, and no changes in triglyceride levels in the control group (K +) and the treatmentgroup. Keywords: tomato juice, Triglycerides, 9-oxo-ODA
Pengaruh Lama Pemberian Distres Kronis terhadap Aktivitas Sel Osteoklas pada Tulang Alveolar Tikus Sprague-Dawley (The Effect of Chronic Distress Duration to Osteoclast Cells Activity in Alveolar Bone of Sprague-Dawley Rats) Maria Devitha; Didin Erma Indahyani; Happy Harmono
Pustaka Kesehatan Vol 3 No 3 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Excessive and prolonged distress (chronic) can cause damage to periodontal tissues, specifically alveolar bone resorption. Alveolar bone resorption is caused by osteoclast cells activity. The purpose of the research is to determine effect of chronic distress duration to osteoclast cells activity in alveolar bone of male Sprague-Dawley rats attain the age of 3-4 months. The rats were given an electric shock stressor (Electrical Foot Shock) as distress chronic for 7, 14, and 28 days. Stressors were given for 30 minutes at 9 a.m every day during the treatment period. The amount of electrical current is 2-8 mA, 48 V voltages and 0.5 Hz frequency. Osteoclasts activity were observed histologically with 400x magnification using osteoclast Bone Interface (OBI) Index by dividing the length of howship's lacunae with osteoclasts. Result showed signifficant difference at 0.00 (p<0.05) beetwen the variable.Activity of osteoclasts in alveolar bone of Sprague-Dawley rats which were exposed to the stressor is increased when compared to the group that were not exposed to electrical shock stressor, but at day 14 decreased when compared to the other treatment groups. Keyword : chronic distress, osteoclast activity, alveolar bone
Pengaruh Lama Distres Kronis Terhadap Perubahan Jumlah Sel Osteoklas Pada Tulang Alveolar Tikus Sprague dawley (The Effect of Chronic Distres Duration on Osteoclast Changes in Alveolar Bone of Sprague dawley Rats) Cicik Khilar Rizqi; Happy Harmono; Raditya Nugroho
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Chronic distress is a psycho-physiological reaction of stimuli that disrupt homoestasis which cause damage to the body, one of kind is an electrical shock stressor. The distress can increase catecholamines level in considerable amounts and allegedy it produce pro-inflamatory cytokines. Cytokine will affect the Hypoyhalamic pituitary adrenal (HPA) axis to produce cortisol and also act as negative control of cytokines. In this assumption, cytokines can increase bond of osteoclast formation key mediator which is RANKL with its receptor (RANK) to produce osteoclasts. This study was an experimental laboratories research using 8 weeks old Sprague dawley rats with body weight 200-250 gram as samples to observe increase of the number of osteoclasts in the alveolar bone. A total of 32 samples were divided into 4 groups: the rats group exposed with electrical shock stressor  for 0, 7, 14, and 28 days. Then samples were processed by tissue processing  until it’s ready to observe using an electron microscope with 400x magnification. The results showed  that there were significant differences (p <0.05) between groups. It is concluded that the number of osteoclasts alveolar bone osteoclast were increased in Sprague dawley rats that experienced chronic distress. Keywords: stress/distress, cathecolamines, cytokine, cortisol,osteoclast
Evaluation of orthodontic tooth movement by 3D micro-computed tomography (µ-CT) following caffeine administration H. Herniyati; Happy Harmono; Leliana Sandra Devi; Sri Hernawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 1 (2019): March 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.876 KB) | DOI: 10.20473/j.djmkg.v52.i1.p1-7

Abstract

Background: The compressive strength of orthodontic tooth movement will be distributed throughout the periodontal ligament and alveolar bone, resulting in bone resorption on the pressure side and new bone formation on the tension side. Caffeine, a member of the methyl xanthine family, represents a widely-consumed psychoactive substance that can stimulate osteoclastogenesis through an increase in RANKL. A 3D Micro-Computed Tomography (µ-CT) x-ray device can be used to measure orthodontic tooth movement and changes in periodontal ligament width. Purpose: The purpose of this research was to analyze the effects of caffeine on the distal movement distance of two mandibular incisors using 3D µ-CT. Methods: The research subjects (guinea pigs) were randomly divided into four groups. Of the two control groups created, one received two weeks of treatment and the other three weeks. The members of these two control groups were subjected to orthodontic movement but received no caffeine. Meanwhile, the other two groups were treatment groups whose members also received either two or three weeks of treatment. In these two treatment groups, the subjects were subjected to orthodontic movement and received a 6 mg/500 BM dose of caffeine. The orthodontic movement of the subjects was induced by installing a band matrix and orthodontic bracket on each mandibular incisor to move distally by means of an open coil spring. Observations were then conducted on days 15 and 22 with µ-CT x-rays to measure the distal movement distance of the two mandibular incisors and the width of the periodontal ligament. Results: The administration of caffeine increased the tooth movement on day 15 (p<0.05) and day 22 (p<0.05). The increase in the tooth movement on day 22 was greater than that on day 15 (p<0.05). The width of the periodontal ligament on the pressure side of the treatment groups experienced greater narrowing than that of the control groups (p<0.05). Meanwhile, the width of periodontal ligament on the tension side of the treatment groups widened more than that of the control groups (p<0.05). Conclusion: µ-CT x-ray can be used to evaluate the extent of orthodontic movement in addition to the width of the mandibular incisor periodontal ligament during orthodontic tooth movement. Moreover, it has been established that the administering of caffeine can improve orthodontic tooth movement.
Daya Hambat Ekstrak Biji Kopi Robusta (Coffea canephora) terhadap Pertumbuhan Fusobacterium nucleatum Anisa Nur Hakima; Tantin Ermawati; Happy Harmono
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 17 No 1 (2020)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v17i1.23608

Abstract

Fusobacterium nucleatum is a bacteria found in normal flora of the oral cavity that plays a role in the occurrence of periodontal disease. The prevalence of periodontal disease in Indonesia reaches 70%. One treatment by using medicinal plants is robusta coffee beans. Robusta coffee beans contain volatile acids, chlorogenic acids, caffeine acids, phenols and caffeine suspected to be antibacterial. The aim was to determine the inhibition of coffee robusta extract on growth of Fusobacterium nucleatum. This study used disc diffusion method with 4 samples in each study group. The study group consisted of 6 treatment groups (12,5%, 25%, 50% and 100% coffee robusta extract), positive control group (chlorhexidine gluconate 0,2%), and negative control group (sterile aquades). Data were analyzed using One Way Anova test and LSD (Least Significant Difference) test. Coffee robusta extract has the ability to inhibit the growth of Fusobacterium nucleatum. The concentration of coffe robusta extract 100%, 50%, 25%, 12,5% have same ability to inhibit the growth of Fusobacterium nucleatum.
Efektivitas Gel Ekstrak Biji Kopi Robusta (Coffea canephora) terhadap Peningkatan Jumlah Fibroblas pada Penyembuhan Luka Pasca Gingivektomi Nailah Rahmadani; Melok Aris Wahyukundari; Happy Harmono
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 19 No 1 (2022)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v19i1.30694

Abstract

Gingivectomy is a surgical procedure aimed at eliminating pseudo pocket in gingival hyperplasia, and also to obtain a good gingival margin aesthetic. The process of wound healing after gingivectomy takes a long time. Fibroblasts are cells that takes an important role in the process of wound healing. One alternative treatment giving medication by applying Robusta coffee bean extract gel which acts as an anti-inflammatory and antioxidant in the wound healing process. The objective was to determine the potential and optimal concentration of Robusta coffee bean extract gel to increase the number of fibroblast cells in post-gingivectomy wound healing phase. This study used 32 male wistar rats were divided into 4 groups. All male wistar rats were treated with gingivectomy in the left mandibular first molar tooth area. The first group as a negative control did not apply any drug, the second group applied 40% robusta coffee extract gel, the third group applied 50% robusta coffee extract gel, and the fourth group 60% robusta coffee extract gel was applied. On the 3rd and 7th day, the experimental animals in all groups were decapitated to do a histological examination of fibroblasts in post-gingivectomy wounds phase. The result showed that applied robusta coffee extract gel present higher number of fibroblasts than the negative control group. The group of 40% dan 50% shows a significant difference of the number of fibroblasts on the 3rd and 7th day. The conclusion was robusta coffee bean extract (Coffea canephora) potential to increase the number of fibroblasts in wound healing phase after gingivectomy, with an effectivity concentration of 40% and 50%.
The effect of Porphyromonas gingivalis induction on neutrophil’s superoxide production Nahdiya Fitriyana; Yuliana MD Arina; Happy Harmono; IDA Susilawati
Journal of Dentomaxillofacial Science Vol. 12 No. 3 (2013): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v12i3.370

Abstract

Microbicidal mechanism with producing free radical superoxide is one of neutrophil response to the bacterialinvasion. When overproducted, the free radical superoxide greater than antioxidan defense system and destroyed thecell itself. P.gingivalis had been knowed as the major bacteria that induce periodontal inflammation. These bacteriacan stimulated neutrophil activities. This was aimed to determinate the superoxide production from neutrophilstimulated with P.gingivalis. Neutrophil as samples were isolated from subject who fulfill the selected criteria. Theintracelluler superoxide production was determinated by nitroblue tetra zolium (NBT)test and the extracellulerproduction by spectrophotometer with wave length 580 nm. The result showed there was superoxide production fromneutrophil stimulated by P.gingivalis. It was concluded that the stimulation of P.gingivalis on neutrophil can promotesuperoxide production both intracelluler and extracelluler.
IL-17 plasma levels and erythrocyte sedimentation rate on oral candidiasis animal model Erna Sulistyani; Iin Eliana Triwahyuni; Happy Harmono; Lisa Miftakhul Janna; Saikha Adila Azzah; Muchamad Ziyad Afif; Ainunnusak Ayuningtyas
Dental Journal Vol. 55 No. 4 (2022): December 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i4.p209-214

Abstract

Background: A study with female animal models is important because the system immune of females is remarkably different from the male because of interaction between sex hormone and immune system. Interleukin-17 (IL-17) plays an important role in immune response toward Candida albicans (C. albicans) infection and Erythrocyte Sedimentation Rate (ERS) is an easy and sensitive test to assess the inflammation. Purpose: This study aimed to evaluate C. albicans infection, analyse the IL-17 levels and ESR in a female animal model of oral candidiasis. Methods: Female Wistar rats were used as oral candidiasis animal model. The rats divided into three groups (pre-treatment group (P0), 5th-day post-treatment group (P1) and 8th-day post- treatment group (P1). Each group consists of six rats. After the adaptation period, the P0 was sacrificed. The drinking water of the P1 and P2 was added tetracycline HCl 500mg /1L. On the day before and after inoculation C. albicans, Methylprednisolone was injected. Ten minutes before inoculation with 0.3 ml C. albicans 9.4 x 107/mL, rats were sedated by CPZ 0.7 mg IM. The rats in P1 group were sacrificed after five days and in P2 were sacrificed eight days after inoculation. The IL-17 plasma levels measured by enzyme-linked immunosorbent assay (ELISA), decreased on the 5th day but not on the 8th-day post-treatment. The obtained data were analyzed by parametric and non-parametric tests according to normality and homogeneity of the data with p<0.05. Results: The colony forming unit (CFU) of C. albicans collected over the mouth on increased almost 8-fold and on 8th-day post-treatment almost 3-fold compared with pre-treatment. The ESR increased on the 8th day but not on the 5th-day post-treatment. Conclusion: The IL-17 level was decreased on the 3rd day, ESR increased on eight days after inoculation of C. albicans in female rats’ model of oral candidiasis.
Co-Authors Ainunnusak Ayuningtyas Anisa Nur Hakima Arina, Yuliana Mahdiyah Daat Cicik Khilar Rizqi Desi Sandra Sari Didin Erma Indahyani Dina Nuur Rosyiidah Dwi Prijatmoko Erna Sulistyani Fany, Manta Fawwazi, Rafif Hanif Hakima, Anisa Nur Herniyati Herniyati Herniyati Hestieyonini Hadnyanawati I Dewa Ayu Ratna Dewanti I Dewa Ayu Susilawati IDA Susilawati Iin Eliana Triwahyuni Kartikasari, Dania Leliana Sabdra deviade Putri Leliana Sandra Devi Ade Putri Lisa Miftakhul Janna Maria Devitha Masari, Al Mayangsari, Zahra Melok Aris Wahyukundari Melok Aris Wahyukundari, Melok Aris Morauli, Fatricia Muchamad Ziyad Afif Nahdiya Fitriyana Nailah Rahmadani Nailufar, Khanun Perwitasuci, Brigitta Damai Prameswari, Syefira Putri Prasetya, Rendra C. Prihatiningrum, Berlian Pudjiastuti, Peni Pujiana Endah Lestari Qurratul Aini Wahyu Pratiwi Raditya Nugroho Riclas Yusuf Punta Wibisono Saikha Adila Azzah Sari Setyaningsih Setyaningsih, Sari Sholehah, Almaratus Sri Hernawati Suhartini Suhartini Tantin Ermawati Tantin Ermawati Utha, Maria Fransisca Wanodyo, Ari Tri Wulandari, Widya Nurfitriastuti Yuliana Mahdiyah Daat Arina Zahara Meilawaty Zahara Meilawaty Zahreni Hamzah