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The effects of ethyl acetate fraction of Ananas Comosus (L.) Merr. of tongue cancer cell growth inhibition Supriâs Clone-1, invitro Martina, Maureen; Oewen, Roosje Rosita; Riyanti, Eriska; Syawqie, Achmad; Supriatno, S.
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Ananas comosus (L.) Merr has several purposes which include antioxidant and anti-inflammatory activity that shows some pharmacological effects and the subject of anti-cancer or anti-cancer supporting material. The research objective was to analyze the effects of ethyl acetate fraction of Ananas comosus (L.) Merr. of tongue cancer cell growth inhibition Supriâs clone-1 (SP-C1). This type of study was a research laboratory. Next, cell growth inhibition testing by the ethyl acetate fraction of Ananas comosus (L.) Merr. with various concentrations (0; 62.5; 125; 250; 250; 500 and 1000 microgram/ml) using the MTT assay test. Growth barriers identified by Biorad microplate tool reader with a wavelength of 540 nm. The number of SP-C1 cells examined was 2 x 104 cells/wells with incubation time 24 and 48 hours. Data were analyzed using a two-ways ANOVA followed by post hoc test (LSD test) with 95% significance level. The results showed ethyl acetate fraction of Ananas comosus (L.) Merr. able to inhibit the growth of cancer cells SP-C1. Various concentrations of ethyl acetate fraction of pineapple were highly significant, meaning that the concentration effect on cell growth of SP-C1. Similarly, incubation time effect on the growth of SP-C1 cells that were very meaningful. The biggest obstacle effect of ethyl acetate fraction of Ananas comosus (L.) Merr. occurred at a concentration of 1000 ug/ml (43.45%) with an incubation time of 48 hours. Conclusion of this study was the fraction of ethyl acetate Ananas comosus (L.) Merr. has the effect of inhibiting the growth of cancer cells SP-C1.

Ekspresi Reseptor-Estrogen pada Adenoma Pleomorfik Parotis Yazid, Achmad Syawqie
Majalah Kedokteran Bandung Vol 42, No 4
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Adenoma pleomorfik parotis (APP) merupakan tumor jinak yang umum terjadi pada kelenjar liur parotis, memilikiÂ perilaku biologi beragam, yaitu sering rekurens setelah dioperasi, dapat berubah menjadi ganas, juga kadangÂ bermetastasis ke kelenjar getah bening atau ke organ lain. Selayaknya untuk tumor APP ini dilakukan pencarianÂ bentuk terapi alternatif lain selain terapi bedah yang diduga penyebab tingginya rekurensi tumor. Terapi alternatifÂ lain dapat berupa hormonal yang memerlukan data dasar ekspresi reseptor estrogen oleh sel tumor APP. UntukÂ mengungkapkan ekspresi reseptor estrogen oleh sel tumor APP, dilakukan penelitian retrospektif eksperimentalÂ laboratoris pemeriksaan imunohistokimia teknik LsAB/labelled strepavidine-biotine terhadap ekspresi estrogenÂ receptor (ER) pada 50 sampel tumor APP di Bagian Patologi Anatomi FKUP/RSHS pada tahun 1998â€“1999.Â Pengamatan ditujukan pada kelompok penyebaran tingkat imunoekspresi ER oleh sel tumor APP: 0=negatif;Â +1=fokal (>20%); +2=heterogen (20â€“50%); dan +3=difus (>50% sel tumor imunoreaktif). Hasil menunjukkanÂ 40/50 sampel tumor APP (80%) imunoreaktif terhadap ER dengan komposisi imunoekspresi: 67,5% heterogenÂ (27/40) dan 32,5% difus (13/40). Pola pulasan homogen teramati pada inti sel tumor. Sepuluh kasus memperlihatkanÂ hasil negatif (negatif 8 kasus dan fokal 2 kasus). Komponen jaringan tumor APP yang positif meliputi sel epitelÂ duktal/tubuloduktal, area miksomatosa, dan hiperselular. Simpulan, tumor APP berperan sebagai target hormonÂ estrogen dan besar kemungkinannya responsif terhadap terapi hormonal. [MKB. 2010;42(4):181â€“6].Kata kunci: Adenoma pleomorfik, estrogen receptor, tumor parotisEstrogen-Receptor Expression in Parotid Pleomorphic AdenomaParotid pleomorphic adenoma (PPA) is a benign tumor frequently found in parotid salivary gland. This tumor hasÂ various biological behaviors including recurrence after surgery, ability to transform into the malignant form, andÂ occasionally, metastasizes to lymph nodes or other organs. For this PPA tumor, it is appropriate to look for alternative therapy forms other than surgery which is assumed to be the cause of the high tumor recurrence. Other alternativesÂ may include hormonal therapy that needs baseline data on estrogen-receptor expression by PPA tumor cells. ToÂ collect the baseline data, a laboratory experimental retrospective study on immunohistochemical study usingÂ LsAB/labelled strepavidine-biotine technique of estrogen receptor (ER) receptor on 50 PPA tumor samples at theÂ Anatomic Pathology Department of the Faculty of Medicine, Padjadjaran University/Hasan Sadikin GeneralÂ Hospital during 1998â€“1999 has been conducted. The observation is focused on the grouping of ERÂ immunoexpression level spread by PPA tumor cells: 0=negative; +1=focal (>20%); +2=heterogeneous (20â€“50%);Â and +3=diffuse (>50% immunoreactive tumor cells). The results showed that 40/50 PPA tumor samples (80%) wereÂ immunoreactive towards ER with an immunoexpression composition of: 67.5% heterogeneous (27/40) and 32.5%Â diffuse (13/40). A homogenous smear pattern was observed in tumor cell nucleus. Ten cases showed negative resultsÂ (8 cases negative and 2 cases focal). The positive PPA tumor tissue components included ductal /tubuloductalÂ epithels, mixomatose and hypercellullar areas. In conclusion, PPA tumor plays the role as target of estrogen hormoneÂ and there is a high possibility that it is responsive to hormonal therapy. [MKB. 2010;42(4):181â€“6].Key words: Estrogen receptor, parotid tumors, pleomorphic adenomaÂ DOI: http://dx.doi.org/10.15395/mkb.v42n4.34

Hubungan Kadar sCD14, Jumlah Streptococcus Mutans, pH-Dapar, Fluor di Dalam Saliva dengan Kejadian Karies pada Anak Karyawan PTPN VIII Pangalengan Usia 12-15 Tahun Dudi Aripin; Setiawan Natasasmita; Richata Fadil; Achmad Syawqie; Wazillah Nasserie
Indonesian Journal of Applied Sciences Vol 1, No 3 (2011)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijas.v1i3.1881

Dental caries is a multifactorial infection where there are four main etiological factors, i.e. salivary and teeth bacteria, carbohydrate, and time. The interaction among those factors can be described as four overlapped circles. The salivary sCD14 protein plays an important role in protecting hard and soft oral tissues from infection by pathogenic bacteria. There is a relationship between the presence of sCD14 in saliva of active caries patients and in caries-free patient which leads to an assumption that this salivary protein may play an important role in caries development process. To look for an evidence of that assumption, a study on the relationship between salivary sCD14 level, number of Streptococcus mutans, Buffer pH, fluor in the saliva and dental caries incidence. The objective is to see the difference in the salivary sCD14 level, number of Streptococcus mutans, Buffer PH and fluor in the saliva as well as to analyze the correlation of those factors with high caries incidence. This study is an analytical observational study using “case control” design with children of PTPN VIII Pangalengan employees with an age range of 12-15 years old as the sample. The sample size was determined using the rule of thumb with a minimum of 80 samples. The selection of subjects was conducted using simple random sampling and the analysis in this study is performed using bivariate (t-test and X2 test) and multivariate (multiple logistic regression analysis). To determine the cut off point, an ROC (Receiver Operating Characteristic) curve. The significance of the test results was determined based on a p value of < 0.005. The results of the study show that the sCD14 level in low caries subjects is higher than in the high caries subjects which is statistically and significantly different. The number of Streptococcus mutans bacteria in low caries subjects is fewer compared to the high caries subjects, which difference is statistically significant. Based on the results of the multiple logistic regression analysis, there is a correlation between sCD14 level, number of Streptococcus mutans, buffer-pH, fluor in saliva and the high incidence of caries. Those four factors strongly influence the high caries incidence It is concluded that there is a strong correlation between sCD14 level number of Streptococcus mutans, buffer-pH, fluor in the saliva and high caries incidence. The risk factor with the highest influence towards caries incidence in this study is the fluor concentration in saliva.****Karies gigi merupakan penyakit infeksi multifaktorial yang disebabkan oleh empat faktor utama sebagai etiologi, yaitu gigi dan saliva, bakteri, karbohidrat dan waktu. Paduan faktor tersebut dapat digambarkan sebagai empat lingkaran yang saling tumpang tindih. Protein sCD14 di dalam saliva memegang peran penting dalam melindungi jaringan lunak dan keras di rongga mulut dari infeksi oleh bakteri patogen. Terdapat hubungan antara keberadaan sCD14 di dalam saliva antara penderita karies aktif dengan penderita bebas karies, hal ini menimbulkan dugaan bahwa protein sCD14 mungkin berperan penting dalam proses perkembangan karies. Untuk mengungkapkan hal tersebut diperlukan penelitian tentang hubungan kadar sCD14, jumlah Streptococcus mutans, pH-bufer, fluor di dalam saliva dengan kejadian karies. Tujuannya yaitu untuk melihat perbedaan kadar sCD14, jumlah Streptococcus mutans, pH-bufer, fluor di dalam saliva serta menganalisis hubungan faktor tersebut dengan kejadian karies tinggi. Penelitian ini bersifat observasional analitik dengan rancangan “kasus kontrol”. Sampel penelitian adalah anak karyawan PTPN VIII Pangalengan usia 12-15 tahun. Ukuran sampel ditentukan secara ‘rule of thumb’ dengan jumlah sampel minimal 80. Pemilihan subjek dilakukan secara simple random sampling. Pada penelitian ini digunakan analisis bivariable (uji t dan uji Chi Kuadrat), multivariable (regresi logistik ganda), dan untuk menentukan cut off point digunakan kurva ROC (Receiver Operating Characteristic). Kemaknaan hasil uji ditentukan berdasarkan nilai p≤0,005. Hasil penelitian kadar sCD14 pada karies rendah lebih tinggi bila dibandingkan dengan karies tinggi, secara statistik berbeda dan bermakna. Jumlah Streptococcus mutans pada karies rendah lebih sedikit bila dibandingkan dengan karies tinggi, secara statistik berbeda sangat bermakna. Berdasarkan hasil analisis regresi logistik ganda menunjukkan adanya hubungan kadar sCD14, jumlah Streptococcus mutans, pH-bufer, fluor di dalam saliva dan kejadian karies tinggi, keempat faktor tersebut berpengaruh kuat terhadap kejadian karies tinggi. Simpulan terdapat hubungan yang kuat antara kadar sCD14, jumlah Streptococcus mutans, pH-bufer, fluor di dalam saliva dengan kejadian karies tinggi. Faktor risiko yang berpengaruh paling kuat terhadap kejadian karies pada penelitian ini adalah konsentrasi fluor di dalam saliva.

The effects of ethyl acetate fraction of Ananas Comosus (L.) Merr. of tongue cancer cell growth inhibition Supri’s Clone-1, invitro Maureen Martina; Roosje Rosita Oewen; Eriska Riyanti; Achmad Syawqie; S. Supriatno
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Faculty of Dentistry Universitas Padjadjaran

Ananas comosus (L.) Merr has several purposes which include antioxidant and anti-inflammatory activity that shows some pharmacological effects and the subject of anti-cancer or anti-cancer supporting material. The research objective was to analyze the effects of ethyl acetate fraction of Ananas comosus (L.) Merr. of tongue cancer cell growth inhibition Supri’s clone-1 (SP-C1). This type of study was a research laboratory. Next, cell growth inhibition testing by the ethyl acetate fraction of Ananas comosus (L.) Merr. with various concentrations (0; 62.5; 125; 250; 250; 500 and 1000 microgram/ml) using the MTT assay test. Growth barriers identified by Biorad microplate tool reader with a wavelength of 540 nm. The number of SP-C1 cells examined was 2 x 104 cells/wells with incubation time 24 and 48 hours. Data were analyzed using a two-ways ANOVA followed by post hoc test (LSD test) with 95% significance level. The results showed ethyl acetate fraction of Ananas comosus (L.) Merr. able to inhibit the growth of cancer cells SP-C1. Various concentrations of ethyl acetate fraction of pineapple were highly significant, meaning that the concentration effect on cell growth of SP-C1. Similarly, incubation time effect on the growth of SP-C1 cells that were very meaningful. The biggest obstacle effect of ethyl acetate fraction of Ananas comosus (L.) Merr. occurred at a concentration of 1000 ug/ml (43.45%) with an incubation time of 48 hours. Conclusion of this study was the fraction of ethyl acetate Ananas comosus (L.) Merr. has the effect of inhibiting the growth of cancer cells SP-C1.

Oral Manifestations in Patients with Down's Syndrome and Management in Prosthodontics Achmad Syawqie; Jennifer Putri; Stephanie Yessica; Nuroh Najmi
Jurnal EduHealth Vol. 14 No. 01 (2023): Jurnal eduHealth, Periode Januari-Maret, 2023
Publisher : Sean Institute

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Down syndrome is an autosomal disorder associated with involving mental and physical changes. The types of craniofacial phenotypes and dental anomalies in individuals with Down syndrome have been described extensively, but further elaboration of their impact of oral and craniofacial conditions still needs to be understood. In addition, in prosthodontic, oral rehabilitation with complete dentures and implants in patients with Down's syndrome still needs to be studied. The aim of this review is to describe that the oral and craniofacial manifestations may occur in patients with Down syndrome, and determine the effectiveness of oral rehabilitation treatments in patients with Down syndrome. The article was conducted of two databases and were limited to the period from January 2016 to February 2022 with a combination of the following keywords: “Down Syndrome”, “oral manifestation”, “implant”, “prosthodontic treatment”. The results obtained were 194 articles found at the beginning of the search in both databases, and the 16 full text articles were selected for further review and discussion. The results of this study shows that Down syndrome causes several oral and craniofacial manifestations, dental caries risk, higher periodontal disease, dental abnormalities, soft and occlusal tissue, and the presence of a craniofacial complex. In prosthodontics, the choice of using removable dentures or implants must be adjusted to each patient's condition. Down syndrome is an autosomal disorder that has oral and craniofacial manifestations that must be a concern for health practitioners, especially dentists. In prosthodontics, oral rehabilitation with either complete dentures or implants in patients with Down's syndrome is the right choice, but it is necessary to consider the complications that may occur.

The Role Of Entamoeba Gingivalis In Periodontal Disease: A Literature Study Emma Rachmawati; Rizky Farida Berlian; Ina Hendiani; Nuroh Najmi; Achmad Syawqie
Jurnal EduHealth Vol. 14 No. 02 (2023): Periode April-June, 2023
Publisher : Sean Institute

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Background: Periodontal disease can affect the supporting structures of the teeth and can cause tooth loss. Periodontal disease is caused mainly by bacteria in dental plaque, but protozoa can also be found in deep pockets. The most common protozoa existing in periodontal disease is Entamoeba gingivalis. This study aims to determine the role of E. gingivalis in periodontal disease. Method: This research was a rapid review which is a method of synthesizing and assessing existing research. Articles were searched using the PubMed database from February 2021 to April 2021 with inclusion criteria in English from 2010 to 2020 and discussing the presence of E. gingivalis in periodontal disease. Result: Articles that comply with the inclusion criteria of this study are 11 articles. The study designs included case-control, cross-sectional, cohort, and case series. All articles concluded that E. gingivalis was found in patients with periodontal disease. Conclusion: The presence of E. gingivalis in large numbers in inflamed periodontal tissues indicates that E. gingivalis has a role in the course of periodontal disease.

Insights Into Oral Manifestations As Diagnostic Indicators Of Viral Infections : A Literature Review Sovira, Gita Dwi Jiwanda; Achmad Syawqie
Jurnal EduHealth Vol. 15 No. 04 (2024): Jurnal EduHealt (inpres), Year 2024
Publisher : Sean Institute

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Background: Infectious diseases, particularly viral infections, represent a major global health concern, with significant implications for oral health. Despite their clinical relevance, oral manifestations of viral infections are often underdiagnosed due to their complex and varied presentations. Early recognition of these manifestations can aid in timely diagnosis and management, improving overall patient outcomes. Objective: This study aims to systematically review the oral manifestations associated with viral infections to provide a comprehensive reference for healthcare professionals in diagnosing and managing such conditions. Methods: A systematic review was conducted using five databases, including PubMed and Scopus, to identify relevant studies published between 2014 and 2024. Keywords such as "oral manifestation" and "viral infection" were used. Eligible studies were selected based on predefined inclusion and exclusion criteria, focusing on articles in English with clinical relevance to oral health. Data were extracted independently by multiple authors and analyzed to identify patterns and significant findings regarding oral manifestations of viral infections. Results: The review identified 133 articles, with 24 studies meeting the inclusion criteria. Key findings revealed that human herpesviruses, including HSV-1, HSV-2, VZV, EBV, CMV, HHV-6, HHV-7, and HHV-8, frequently present with distinct oral lesions. These lesions range from vesicles and ulcers to gingival overgrowth and vascular tumors. The study highlights the diagnostic importance of oral findings, particularly in immunocompromised patients. Conclusion: Oral manifestations are critical diagnostic indicators of viral infections and provide an opportunity for early disease recognition. A thorough understanding of these manifestations can enhance clinical diagnosis, reduce healthcare costs, and improve patient outcomes. Future research should focus on developing diagnostic algorithms incorporating oral and systemic findings for better clinical utility.

The role of virtual reality haptic technology in dental education: A systematic review Abraari, Mohammad Agiel; Syawqie, Achmad; Nur'aeny, Nanan
Padjadjaran Journal of Dental Researchers and Students Vol 9, No 3 (2025): October 2025
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v9i3.65494

ABSTRAKIntroduction: Dental students are required to develop strong clinical motor skills, but conventional training tools such as mannequins often lack realistic sensory feedback. Haptic virtual reality (VR) technology offers a modern solution by providing tactile simulation, though its effectiveness compared to traditional methods remains unclear. This study aims to evaluate the effectiveness of haptic VR in improving motor skills, knowledge, and confidence among dental students. Methods: A systematic review was conducted following PRISMA guide lines, including RCT or observational studies on haptic VR in dental education from PubMed, Scopus, and ScienceDirect databases. Data were analyzed narratively due to study heterogeneity. Results: A total of 12 studies show that haptic VR is effective for training procedures such as caries preparation, scaling, veneer preparation, and pulpotomy. Students demonstrated improved hand-eye coordination, procedural understanding, and confidence. However, effectiveness depends on device quality, user experience, and integration into the curriculum. Conclusions: Haptic VR cannot fully replace traditional methods but serves as a valuable complementary tool, especially in endodontics, periodontics, and as a supplementary aid in conservative dentistry, prosthodontics, and pediatric dentistry.KEY WORDS: haptic virtual reality, motoric skill, dentistryPeran teknologi haptic virtual reality dalam pendidikan kedokteran gigi: Ulasan sistematikABSTRACTPendahuluan: Mahasiswa kedokteran gigi membutuhkan keterampilan motorik klinis yang baik, namun pelatihan konvensional seperti manekin memiliki keterbatasan dalam memberikan umpan balik sensorik. Teknologi haptic virtual reality (VR) menawarkan solusi modern dengan stimulasi taktil yang realistis, namun efektivitasnya masih perlu dikaji secara sistematis. Studi ini bertujuan mengevaluasi efektivitas haptic VR dalam meningkatkan keterampilan motorik, pengetahuan, dan kepercayaan diri mahasiswa. Metode: Penelitian menggunakan metode systematic review dengan pedoman PRISMA, mencakup artikel RCT atau observasional mengenai haptic VR dalam pendidikan kedokteran gigi dari database PubMed, Scopus, dan ScienceDirect. Analisis dilakukan secara naratif karena heterogenitas studi. Hasil: Terdapat 12 artikel yang menunjukkan haptic VR efektif dalam pelatihan prosedur seperti preparasi karies, scaling, veneer, dan pulpotomi. Mahasiswa mengalami peningkatan koordinasi tangan-mata, pemahaman prosedur, dan kepercayaan diri. Efektivitas dipengaruhi oleh kualitas perangkat, pengalaman pengguna, dan integrasi dalam kurikulum. Simpulan: haptic VR belum menggantikan metode tradisional, namun berpotensi sebagai alat bantu yang efektif, khususnya dalam pelatihan endodontik, periodontik, konservasi, prostodontik, dan kedokteran gigi anak.KATA KUNCI: haptic virtual reality, keterampilan motorik, pendidikan kedokteran gigi

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