Garuda - Garba Rujukan Digital

Antioxidant Capacities of Holothuria Sea Cucumbers Murniasih, Tutik; Putra, Masteria Yunovilsa; Pangestuti, Ratih
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i2.217

Sea cucumbers are a potential source of bioactive metabolites for nutraceutical, pharmaceutical and cosmeceutical products. In this study we investigated the antioxidant activityof several extracts of sea cucumbers, Holothuria scabra, Holothuria atra, Holothuria leocospilota, and Holothuria excellens collected from Lombok Island. Phytochemical screening revealed the presence flavonoid, terpenoids, phenol, saponin, alkaloid, anthraquinone and glycoside in the extracts of Holothuria sea cucumbers. Radical scavenging effects on the DPPH model were evaluated. The crude extract of H. leocospilota and H. atra showed the highest potential antioxidant capacity. H. atra extract was separated by column chromatography silica gel eluting with a gradient system of increasing polarity. Fraction 7 of H. atra extract showed significantly inhibited radical scavenging activity (35.3487 %) at concentration 1 mg/ml. However, these are preliminary findings and further studies will have to focus on the isolation of bioactive substances derived from H atra and identify other health benefit effects of H. atra.

CARBON SOURCE OPTIMIZATION FOR ANTIBIOTIC PRODUCTION FROM AAPTOS-ASSOCIATED BACTERIA Rhodobacteracea bacterium SP.2.11 Murniasih, Tutik; Bayu, Asep
Marine Research in Indonesia Vol 40, No 2 (2015)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

MarineÂ spongeÂ Aaptos-aaptosÂ isÂ thoughtÂ toÂ produceÂ antimicrobialÂ aaptamineÂ andÂ itsÂ derivatives.Â ToÂ investigateÂ whetherÂ itsÂ associatedÂ bacteriaÂ areÂ inÂ factÂ theÂ producerÂ ofÂ suchÂ bioactiveÂ compounds,Â aÂ studyÂ ofÂ antibacterialÂ compoundsÂ derivedÂ fromÂ Aaptos-associatedÂ bacteriaÂ wasÂ conducted.Â InÂ thisÂ research,Â approximatelyÂ 10Â bacterialÂ coloniesÂ wereÂ isolatedÂ fromÂ theÂ spongeÂ AaptosÂ aaptos.Â AmongÂ theÂ bacteriaÂ isolated,Â theÂ oneÂ thatÂ showedÂ theÂ mostÂ potentialÂ forÂ producingÂ antibacterialÂ compoundsÂ wasÂ RhodobacteraceaÂ bacterium.Â ExtraÂ andÂ intracellularÂ bacterialÂ extractÂ fromÂ thisÂ strainÂ stronglyÂ inhibitedÂ theÂ growthÂ ofÂ pathogenicÂ bacteriaÂ StaphylococcusÂ aureusÂ andÂ VibrioÂ eltor,Â whileÂ wereÂ moderatelyÂ effectiveÂ againstÂ BacillusÂ subtilis.Â OptimizationÂ ofÂ antibacterialÂ activityÂ wasÂ conductedÂ byÂ culturingÂ RhodobacteraceaeÂ bacteriumÂ inÂ variousÂ carbonÂ sourcesÂ suchÂ asÂ glucose,Â lactose,Â amylum,Â molassesÂ andÂ glycerol.Â TheÂ highestÂ productionÂ ofÂ biomassÂ wasÂ obtainedÂ byÂ culturingÂ thisÂ bacteriaÂ inÂ SYPÂ (SeawaterÂ YeastÂ Â Peptone)Â medium,Â enrichedÂ withÂ 1%Â glycerolÂ asÂ theÂ carbonÂ sourceÂ andÂ withÂ aÂ harvestingÂ timeÂ ofÂ aroundÂ 56-104Â hours.Â TheÂ highestÂ activityÂ (8Â U/ml)Â wasÂ reachedÂ whenÂ culturingÂ thisÂ strainÂ inÂ SYPÂ mediumÂ withoutÂ anyÂ addingÂ ofÂ carbonÂ sources.Â DataÂ analysisÂ usingÂ aÂ statisticalÂ toolÂ indicatedÂ thatÂ carbonÂ sourcesÂ addedÂ toÂ mediumÂ doÂ notÂ haveÂ aÂ significantÂ effectÂ onÂ antibacterialÂ activity.Â CharacterizingÂ theÂ compoundÂ responsibleÂ forÂ theÂ antibacterialÂ propertiesÂ willÂ beÂ theÂ topicÂ ofÂ furtherÂ work.KeywordsÂ :Â carbonÂ source,Â RhodobacteraceaÂ bacterium,Â AaptosÂ aaptos,Â associatedÂ bacteriaÂ

BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBON (PAH), PHENANTHRENE BY MARINE BACTERIUM THALASSOSPIRA SP. C.260 Murniasih, Tutik; Lisdiyanti, Puspita; Yopi, -
Marine Research in Indonesia Vol 35, No 1 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) compound that is known to be reported toxic to marine flora and fauna. Remediation of this environmental pollutant using chemical and physical methods causes environmental issues. Bioremediation using marine has been applied to degrade such various PAH compounds. Screening of marine microorganism in degrading this recalcitrant is very importance for bioremediation application in Indonesian waters. The purpose of this study was to screen and isolate bacterial with potential application in biodegradation of phenanthrene and other harmful PAH in marine environments. Several potential bacteria strains were isolated from oil contaminated sea water in Cilacap area. Sequence analysis using 16S rRNA gene marine bacterium strain C.260 showed 96% sequence homology to sequence of Thalassospira sp. In biodegradation of phenanthrene, within 28 days experiments, this bacterium degraded 50% and 99.75% of phenanthrene in medium with and without enrichment with NPK fertilizer respectively. Using sublimation method, this bacterium also degradeds phenothiazine, fluoranthene, and dibenzothiophene.

Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges Artanti, Nina; Maryani, Faiza; Mulyani, Hani; Dewi, Rizna Triana; Saraswati, Vienna; Murniasih, Tutik
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The marine bacteria were cultured in liquid medium under shaking condition were extracted with ethyl acetate. Antidiabetes was measured using inhibition of Î±-glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method.S creening results showed that at sample concentration of 200 Âµg/ml, there was significant Î±-glucosidase inhibitory activity was detected in the extracts of strain sp 7.9 (84% inhibition) and 8.10 (75% inhibition),however the antioxidant activity of these two strains were low only around 30% inhibition, antioxidant activities of other strains were very low.Screening for antibacterial activities using 10Âµl samples showed that extract of strain Sp 8.5was best for Staphylococcusaureus (14 mm inhibition); Sp 7.9 and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 for Pseudomonas aeuriginosa. Based on these results marine bacteria strain Sp 7.9 and Sp 8.10 were selected to be used for further studies in the isolation of bioactive that has potential as antidiabetes and antibacterial.Results of molecular identification conducted by INACC showed that identity of both strain based on BLAST Homology using NCBI database were Bacillus thuringiensis strain Ou2.

The Investigation of Polycyclic Aromatic Hydrocarbon and Oil Degrading Bacteria Isolated from The Marina Port Ancol, Jakarta Bay Lisdiyanti, Puspita; Yopi, Yopi; Murniasih, Tutik
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/45

Polycyclic Aromatic Hydrocarbons (PAHs) as well as crude oil are widespread environmental pollutants. The contamination of air, soil, freshwater (surface water and groundwater), and marine environments by PAHs as well as crude oil has been reported. Of concern to public health is the fact that many PAHs or their metabolites are mutagenic, carcinogenic, or both. North Java coastal area such as Jakarta Bay is the polluted marine area in Indonesia as a result from anthropogenic wastes and the oil spill. Although evaporation and photo-oxidation play an important role in oil detoxification, ultimate and complete degradation is accomplished mainly by marine micro flora, and being dominant in this function. Certain bacteria are well-known could consume and degrade the PAHs as well as crude oil. Therefore investigating the potential PAH and oil degrading marine bacteria is important. In this study, we collected sample from oil polluted area in Marina Port Ancol, Jakarta Bay and isolated four PAH substrates and Arabian crude oil degrading marine bacteria using enrichment method and direct isolation method. As result, 223 strains could degrade PAHs, among these strains, 94 strains could degrade phenanthrene, 23 strains degrade fluoranthene, 92 strains could degrade dibenzotiophen, 14 strains could degrade phenotiazin and 106 isolates degrade crude oil.Key words: polycyclic aromatic hydrocarbons, crude oil, degrading bacteria, bioremediation.

BIODIVERSITY OF HYDROCARBON-PRODUCING MICROALGAE FROM OIL CONTAMINATED IN COASTAL ZONE OF BATAM ISLAND Susilaningsih, Dwi; Siburian, Marlinang Diarta; Murniasih, Tutik
Marine Research in Indonesia Vol 33, No 2 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Addressing to the environmental and energy crises in Indonesia, the new alternative of green-economic-energy sources is required. In this study marine microalgae were isolated from oil-polluted coastal zone in Batam Island for alternative energy sources. The research was carried out by collecting the sea water samples from several peninsulas of Batam Island. The abundance of microalgal diversity was observed. The isolation was done by capillary micropipette technique. The method of specific screening for hydrocarbon-producing microalgae was done using selected media, CO2 rate and light intensity period treatments. The results showed that the Cyanobacteria and diatom occupied the Batams coastal waters. From the isolated samples, 11 strains positively deposit the hydrocarbon in their cells. Further study for determining the isolates and their properties is still in progress.

BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBON (PAH), PHENANTHRENE BY MARINE BACTERIUM THALASSOSPIRA SP. C.260 Murniasih, Tutik; Lisdiyanti, Puspita; Yopi, -
Marine Research in Indonesia Vol 35 No 1 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) compound that is known to be reported toxic to marine flora and fauna. Remediation of this environmental pollutant using chemical and physical methods causes environmental issues. Bioremediation using marine has been applied to degrade such various PAH compounds. Screening of marine microorganism in degrading this recalcitrant is very importance for bioremediation application in Indonesian waters. The purpose of this study was to screen and isolate bacterial with potential application in biodegradation of phenanthrene and other harmful PAH in marine environments. Several potential bacteria strains were isolated from oil contaminated sea water in Cilacap area. Sequence analysis using 16S rRNA gene marine bacterium strain C.260 showed 96% sequence homology to sequence of Thalassospira sp. In biodegradation of phenanthrene, within 28 days experiments, this bacterium degraded 50% and 99.75% of phenanthrene in medium with and without enrichment with NPK fertilizer respectively. Using sublimation method, this bacterium also degradeds phenothiazine, fluoranthene, and dibenzothiophene.

CARBON SOURCE OPTIMIZATION FOR ANTIBIOTIC PRODUCTION FROM AAPTOS-ASSOCIATED BACTERIA Rhodobacteracea bacterium SP.2.11 Murniasih, Tutik; Bayu, Asep
Marine Research in Indonesia Vol 40 No 2 (2015)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Marine sponge Aaptos-aaptos is thought to produce antimicrobial aaptamine and its derivatives. To investigate whether its associated bacteria are in fact the producer of such bioactive compounds, a study of antibacterial compounds derived from Aaptos-associated bacteria was conducted. In this research, approximately 10 bacterial colonies were isolated from the sponge Aaptos aaptos. Among the bacteria isolated, the one that showed the most potential for producing antibacterial compounds was Rhodobacteracea bacterium. Extra and intracellular bacterial extract from this strain strongly inhibited the growth of pathogenic bacteria Staphylococcus aureus and Vibrio eltor, while were moderately effective against Bacillus subtilis. Optimization of antibacterial activity was conducted by culturing Rhodobacteraceae bacterium in various carbon sources such as glucose, lactose, amylum, molasses and glycerol. The highest production of biomass was obtained by culturing this bacteria in SYP (Seawater Yeast Peptone) medium, enriched with 1% glycerol as the carbon source and with a harvesting time of around 56-104 hours. The highest activity (8 U/ml) was reached when culturing this strain in SYP medium without any adding of carbon sources. Data analysis using a statistical tool indicated that carbon sources added to medium do not have a significant effect on antibacterial activity. Characterizing the compound responsible for the antibacterial properties will be the topic of further work.Keywords : carbon source, Rhodobacteracea bacterium, Aaptos aaptos, associated bacteria

BIODIVERSITY OF HYDROCARBON-PRODUCING MICROALGAE FROM OIL CONTAMINATED IN COASTAL ZONE OF BATAM ISLAND Susilaningsih, Dwi; Siburian, Marlinang Diarta; Murniasih, Tutik
Marine Research in Indonesia Vol 33 No 2 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Addressing to the environmental and energy crises in Indonesia, the new alternative of green-economic-energy sources is required. In this study marine microalgae were isolated from oil-polluted coastal zone in Batam Island for alternative energy sources. The research was carried out by collecting the sea water samples from several peninsulas of Batam Island. The abundance of microalgal diversity was observed. The isolation was done by capillary micropipette technique. The method of specific screening for hydrocarbon-producing microalgae was done using selected media, CO2 rate and light intensity period treatments. The results showed that the Cyanobacteria and diatom occupied the Batam's coastal waters. From the isolated samples, 11 strains positively deposit the hydrocarbon in their cells. Further study for determining the isolates and their properties is still in progress.

POTENSI BAKTERI LAUT SEBAGAI SUMBER ANTIBIOTIK BARU PENGHAMBAT STAPHYLOCCUS AUREUS Mardiana, Nur Agustin; Murniasih, Tutik; Rukmi, Widya Dwi; Kusnadi, Joni
Jurnal Teknologi Pertanian Vol 21, No 1 (2020)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

ABSTRAK Penemuan akan antibiotik baru yang semakin menurun dan meningkatnya penyakit yang disebabkan oleh Staphylococcus aureus telah meningkatkan minat terhadap bakteri laut sebagai produsen senyawa antibiotik baru. Tujuan dari penelitian ini adalah mengetahui aktivitas antibakteri dari lima isolat bakteri laut terhadap Staphylococcus aureus dan mengidentifikasi jenis bakteri laut yang memiliki aktivitas antibakteri tertinggi dengan 16S rRNA. Hasil penelitian menunjukkan dari lima isolat bakteri laut, isolat M1.SP3121015.101.a menunjukkan aktivitas antibakteri tertinggi dengan rerata daya hambat sebesar 10,20 mm. Karakterisasi M1.SP3.121015.101.a dilakukan dengan menggunakan 16S rRNA didapatkan cluster gen pada ± 1500 bp. Berdasarkan sekuens isolat M1.SP3121015.101.a memiliki kemiripan dengan Bacillus tequilensis strain K2.4.2 dengan 100% kesamaan. Penelitian ini memberikan manfaat yaitu didapatkannya isolat bakteri laut yang memiliki kemampuan sebagai antibakteri dan dapat digunakan pada penelitian selanjutnya seperti proses isolasi senyawa bioaktif maupun proses optimasi. Kata Kunci: 16S rRNA; Aktivitas Antibakteri; Bakteri Laut; Staphylococcus aureus ABSTRACT A decreasing invention on new antibiotic and increasing disease caused by Staphylococcus aureus has raised increasing interest in marine bacteria as promising producers of new antibiotic agent. The aim of this study is to explore marine bacteria activity to inhibit Staphylococcus aureus . The result showed that among five isolates of marine bacteria, the M1.SP3121015.101.a strain showed the highest antibacterial activity with inhibition zone was 10,20 mm. The characterization ofM1.SP3.121015.101.a was carried out using 16S rRNA, it showed that gene cluster at ±1500 bp. The sequences of M1.SP3121015.101.a have been closes to Bacillus tequilensis strain K 2.4.2 with 100% similarity. This study provides some information about the isolate of marine bacteria that has the highest antibacterial activity and it can be used in further research such as the process of isolation of bioactive compounds and the optimization process.Keywords: 16S rRNA; Antibacterial Activity; Marine Bacteria; Staphylococcus aureus

Title

Found 15 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 15 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 15 Documents
Search