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All Journal Jurnal Veteriner Journal of Parasite Science
Agnes Theresia Soelih Estoepangestie
Department Of Veterinary Public Health - Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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Deteksi Molekuler Gen Penyandi Protein Virb11 pada Brucella abortus Isolat Lokal Asal Pinrang, NTT dan Strain Vaksin Maria Gladis Bupu Maze; Didik Handijatno; Wiwik Tyasningsih; Suwarno Suwarno; Agnes Theresia Soelih Estoepangestie; Rahaju Ernawati
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.177 KB) | DOI: 10.19087/jveteriner.2020.21.4.503

Abstract

Brucellosis in cattle is a disease caused by Brucella abortus due to the reduction in livestock population caused by abortion, stillbirth, weak birth, infertility and sterility. Brucella abortus has several potentialvirulence factors, i.e. virB11 gene that encodes VirB11 protein is an important virulence factor acts as an ATPase for assembling organelles when the bacteria replicate, helping to complete the bacterial cycle and agress to another cells. The aim of this study are to re-identification Brucella abortus and detect virB11 gene as encoding of B. abortus VirB11 protein in local isolates from Pinrang, NTT, strain vaccines S19 and RB51. The isolates Brucella abortus were re-cultured in Brucella agar base and re-identification is followed by microscopic with Gram staining and biochemical tested with urease, citrat, indol and TSIA test. virB11gene was detected with PCR method. The PCR result showed virB11 gene have DNA band 720 bp.virB11 gene are present in local isolates from Pinrang, NTT, strain vaccines S19 and RB51.
The Prevalence and Infection’s Degree of Gastrointestinal Worm of Local Chicken (Gallus domesticus) in Kramat Village, District of Bangkalan, Madura, East Java Indonesia Ellza Agatha Damayanti; Poedji Hastutiek; Agnes Theresia Soelih Estoepangestie; Nunuk Dyah Retno Lastuti; Kusnoto Kusnoto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.378 KB) | DOI: 10.20473/jops.v3i1.16436

Abstract

The study was aimed to know the prevalence and level of gastrointestinal worm investation of local chicken (Gallus domesticus) in Kramat Village, district of Bangkalan, Madura East Java province. The samples used in the study were 100 faeces of kampung chicken. using sedimentation and floating method. Faeces samples were tested, than followed by microscopically test to identify the worm is species. The result showed that in 38 out of 100 samples could be indentified some worm species as followed : Capillaria sp.14 (36,8%), Railletina 7 (18,4%), Hymenolepis 16 (42,1%) and Heterakis gallinarum 1 (2,6%). Using Mann Whitney U-test could be differentiated the level of the infection with an average of 45.53 in cock, while in hen with an average of 55.47. There was no significancy (p>0,05) on the level of worm gastrointestinal infection of different sex of kampung chicken.
Co-Authors . Suwarno Didik Handijatno Ellza Agatha Damayanti Endang Suprihati Kusnoto Kusnoto Maria Gladis Bupu Maze Nunuk Dyah Retno Lastuti Poedji Hastutiek Rahaju Ernawati Wiwik Tyasningsih