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All Journal Jurnal Sain Veteriner Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Journal of Aquaculture Science Journal of Aquaculture and Fish Health Journal of Parasite Science Journal of Marine and Coastal Science Journal of Fisheries & Marine

Kusnoto Kusnoto

Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya

Author-ID : 3558585

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Respon Imun Mencit terhadap Protein 24 dan 71 kDa Toxocara vitulorum dalam Membentuk Antibodi dan Protektifitasnya terhadap Infeksi Buatan Candra Dwi Atma; Kusnoto Kusnoto; Eduardus Bimo Aksono HP.
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study aimed to get 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity on ELISA and to get the protein which able to protect mice against artificial infection of L2 T. vitulorum. This study using mice Balb/c aged 6 to 8 weeks. Proteins isolated were 24 and 71 kDa. Proteins 24, 71 kDa and intestinal homogenates immunized in mice with the addition of adjuvant (1: 1) for 3 times with period of 2 weeks. Two weeks after the last booster, serum drawn from mice tested by Indirect ELISA to determine the value of optical density (OD). The next stage, mice were infected L2 with a dose of 10-17 larvae / g of body weight. The results showed the average OD value by ANOVA Factorial antigen P24 was not significantly different with antigen P71 T. vitulorum. Antigen 24 kDa and 71 kDa with different immunization, both were showed P0 significantly different with P1, P2 and P3. Based on percentage of L2 in the somatic tissue of mice, P0 were showed 79.1% of total number of L2 early infection, whereas the treatment of P1 were showed 0.04%, P2 and P3 showed as much as 0.02% and 0.04%. 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity.

Molecular characterization of Pasteurella multocida pfhaB1 gene fragment from buffalo and cattle isolates from Nusa Tenggara Timur Indonesia Ine Karni; Didik Handijatno; Lucia Tri Suwanti; Kusnoto Kusnoto; Jola Rahmahani; Wiwiek Tyasningsih
Aceh Journal of Animal Science Vol 4, No 2: December 2019
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.4.2.13581

Almost all regions in Nusa Tenggara Timur East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Haemorragic Septicaemia (HS), which is caused by Pasteurella multocida (P. multocida ) Serotypes B: 2. The fragment pfhaB1gene is one of the virulence factors of P. multocida.The objective of this study was to determaine the phylogenetic, homology of P. multocidapfhaB1gene fragment of isolatedfrom Buffalo and Cattle in NTT. The P. multocida isolateswere re-cultured and further microscopic examined the biochemical tests, PCR, sequencing, homology, and phylogenetic relatedness test. P. multocida was observed as gram negative, coccobacilus, no growth on MacConkey Agar, does not produce H2S and gas, nonmotile and indole positive, does not produce urease enzymes, does not use citrate as a carbon source, does not ferment maltose and lactose but it does ferment glucose, sucrose and mannitol. ThepfhaB1gene fragmentfrom buffalo and cattle NTT isolates and also Katha strain vaccine showed DNA band 506 bp. P.multocida isolates from buffalo and cattle in NTT have 91% - 99% score homology with the comparative isolate. The isolate P. multocida from buffalo and cattle in NTT are in one cluster and their phylogenetic relatedness is close to isolates from Iran and India. It is concluded that the pfhaB1gene fragmentof P. multocida from buffalo and cattle isolates have phylogenetic relatedness close and homolog with the other comparative isolates. Keywords: Haemorrhagic Septicaemia; Nusa Tenggara Timur; Pasteurella multocida; pfhaB1gene

The Anthelmintic Effect of Pumpkin Seed (Cucurbita moschata Durch) Infusion on Lethal Death Time of Fasciola giganticaIn Vitro Andriani Dwi Siswarini; Kusnoto Kusnoto; Retno Bijanti
Journal of Parasite Science (JoPS) Vol. 1 No. 1 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Fasciola gigantica is a worm which could infect breeding animal and human. One of the plants that can be used as worm drug is Pumpkin seed (Cucurbita moschata Durch).Pumpkin seed (Cucurbita moschata Durch) has been used in traditional anthelmintic medicine. This research was conducted to determine the anthelmintic effect of Pumpkin seed (Cucurbita moschata Durch) infusion on lethal death time of Fasciola gigantica in vitro. This research used Fasciola gigantica 25-75 mm in length without differentiating their sex. The concentrations of Pumpkin seed (Cucurbita moschata Durch) for treatment of Fasciola gigantica were 25%, 50% and 75%. The negative control used NaCl physiology (0.9 %). ANOVA factorial test showed significant difference among all of the experiment groups (p <0.05). Duncan multiple range test was seen the best treatment was the shortest lethal death time in concentration of 75%.Six hours of the treatment was the best time for making death of the worm. The Pumpkin seed (Cucurbita moschata Durch) in 75% dose was the best dose for making death of the worm. In 6th hours of the treatment in 75% dose administering had the best for making death of Fasciola gigantica. As for the reason, pumpkin seed (Cucurbita moschata Durch) can be use as anthelmintic.

Anthelmintic Activity of Basil Leaves (Ocimum sanctum Linn.) Infusion Against Ascaris suum In Vitro Ogen Sea; Mas'ud Hariadi; Setiawan Koesdarto; Kusnoto Kusnoto; Ngakan Made Rai Widjaja
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Ascaris suum is a parasite nematode that causes infection in swines with high prevalence rates in host populations and usually associated with liver damages called â€œmilk spotsâ€ caused by larvae migration, resulting in organ condemnation. Basil leaves (Ocimum sanctum) phytochemical constituents contains flavonoid, phenol and tannin. Tannins and phenolics are known to interfere with the energy generation in helminth parasites by uncoupling oxidative phosphorylation and also bind to free proteins in the gastrointestinal tract of host animal or glycoprotein on the cuticle of the parasite and leading to death. This study was aimed to determine the activity of basil leaves (Ocimum sanctum) infusion in several concentrations against A. suum in vitro. This research used six treatments and four replications. This research used 10 A. suum in each treatment with four replication. The observations were done at 12, 18, 24, 30 and 36 hours in an incubator at 37oC. Based on the data analysis, basil leaves (Ocimum sanctum) infusion has anthelmintic activity against A. suum in vitro. The greater of the concentration and the longer of time of immersion, will make the death percentage of A. suum become higher. Concentration of basil leaves (Ocimum sanctum) infusion 15% is the effective concentration that can kill 100% of the A.suum during 36 hours of immersion.

Anthelmintic Potential Extract Mango Gadung Seed (Mangifera indica L.) Mecistocirrus digitatus in vitro Ria Nikmatul Jannah; Sri Mumpuni Sosiawati; Sri Chusniati; Kusnoto Kusnoto; Rahmi Sugihartuti; Setiawati Sigit
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

This research aim to attest the potential anthelmintic extract mango seed gadung (Mangifera indica L.) against worms Mecistosirrus digitatus in vitro, the study also aims to determine the effect of immersion time, variasis concentration and the relationship between time and treatment. this study used 210 M. digitatus worm extracted regardless of gender worms. treatment that is given was the concentration extract mango seed gadung was 5%, 7.07%, 10%, 14.14%, 20%, negative control using NaCl physiological and comparison using levamisole Hidrokloride 0.0025 mL. Observations death worm Mecistocirrus digitatus and observations were made at the 6th hour, 12th hour, 18th hour and 24th hour. The results showed that variations in concentration, soaking time and the relationship between soaking time with the treatment affect mortality Mecistocirrus digitatus worms. Test results of the analysis statitistik using factorial Anova and Duncan's Multiple Range Test showed a significant difference (p<0.01) between the time factor, variations in concentration and relations between time and treatment.

The Prevalance of Gastrointestinal Tract Protozoa Using Fecal Examination in Local Chicken(Gallus domesticus) Located in Kramat Village, District of Bangkalan, Bangkalan Regency Talita Yuanda Reksa; Poedji Hastutiek; Hana Eliyani; Kusnoto Kusnoto; Mufasirin Mufasirin
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research is to identify the prevalence of gastrointestinal track protozoa in local chicken (Gallus domesticus) located in Kramat Village, District of Bangkalan, Bangkalan Regency using fecal examination. The number of sample used were 140 including 70 samples from rice fields location and 70 samples from fisheries location. The result showed that 54 (38.6%) local chickens were infected by species of Eimeria; E. acervulina (2.5%), E. brunetti (22.8%), E. maxima (46.8%), E. mitis (1.3%), E. necatrix (22.8%), E. praecox (2.5%), and E. tenella (1.3%). The result was made of 16 (22.9%) local chickens in rice fields location and 38 (54.3%) local chickens in fisheries location. The infection of Eimeria sp. on male local chickens were 24 (34.3%) while on the female local chickens were 30 (42.9%). Chi Square Test showed that there was a highly significant difference toward the prevalence in rice fields and fishery locations (p<0.01), but there was no significant difference toward the prevalence of male and female local chickens (p>0.05).

Anthelmintic Activity Ethanol Extract of Ocimum sanctum Linn. Leaves Against Ascaridia galli In Vitro Vanna Lidya Kharisma; Setiawan Koesdarto; Koesnoto Supriandono; Lucia Tri Suwanti; Sri Agus Sudjarwo; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this research are to determine concentration, exposure time, interaction between concentration and exposure time of ethanol extract of Ocimum sanctum Linn. Leaves to cause death toward Ascaridia galli in vitro, and the value of LC50 and LC90 ethanol extract of Ocimum sanctum Linn. Leaves. Research design that has been used in the research was completely randomized design. This research used 200 samples of Ascaridia galli with length 7-11 cm without differentiating their sex. The concentration ethanol extract of Ocimum sanctum Linn. leaves were 1.25%, 2.5%, 5%, 10%. The control was using CMC-Na 0.5%. Each treatment then being replicated four times. The observation and recording of dead worm were done at 0, 3, 6, 12 and 24 hours. Ascaridia galli were declared dead if there was no movement while disturbed by anatomy tweezers and dipped in slightly warm water (50ºC). The obtained data was analyzed using Anova Factorial and continued with Duncan Multiple Range Test by SPSS for Windows 22. The result were 10% concentration and exposure time for 24 hours caused the most mortality toward Ascaridia galli. Interaction between concentration and exposure time resulted 10% concentration ethanol extract of Ocimum sanctum Linn. leaves in 24 hours caused the most mortality towards Ascaridia galli. Probit analysis was used to calculate the LC50 and LC90 of Ocimum sanctum Linn. leaves. The results were LC50 ethanol extract of Ocimum sanctum Linn. leaves at 6 hours was 14.8%, at 12 hours was 4.8% and at 24 hours was 3.0% and the LC90 at 24 hours was 9.1%.

Identification of Worm In Digestive Tract of Consumed Cattle Egret (Bubulcus ibis) In Ujung Pangkah, Gresik Nisa’ Rachmaningtyas Putri; E Djoko Poetranto; Nunuk Dyah Retno Lastuti; Setiawan Koesdarto; Bambang Sektiari Lukiswanto; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Ujung Pangkah, Gresik was became the most habitat of fowls such as Cattle egret (Bubulcus ibis). The aim of this research were to find out the type of worm that infected digestive tract of Cattle egret (Bubulcus ibis) in Ujung Pangkah, Gresik. The examination of worm in digestive tract was done by dissection and fecal examination. The identification based on morphological of the eggs and worms. The samples of digestive tract organ collected from restaurant that preserve Cattle egret as a main menu. Whereas the fecal sample collected by swab from the intestine. Cattle egret (Bubulcus ibis) has positive infection by worm parasite if there were worm or egg inside the digestive tract. Fecal samples were examined by native, sedimentation, coconut-sugar floating method and EPG counting with Lucient Brumpt. And the worm morphology from dissection of digestive tract would be identify with source book, journal, and discussed with supervisor. The result showed that 20 of digestive tract dissection and 20 fecal samples, there were three types of worm, Contracaecum sp., Cosmocephalus sp., Capillaria sp. and egg of Contracaecum sp. with 200 EPG

Investation of Ectoparasites In Chickens In Village of Kramat District of Bangkalan Regency of Bangkalan Fania Selfiannisa; Suherni Susilowati; Poedji Hastutiek; Lucia Tri Suwanti; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

A study on the infestation of ectoparasites in chicken’s raised in Village of Kramat, District of Bangkalan, Regency of Bangkalan was conducted between July to October 2017. A total of 60 chickens were examined based on their sex and region of the chicken’s body. Identification was carried out on ectoparasites found identified using permanent mounting methods and scraping. The types of ectoparasites were M. gallinae 77%, L. caponis 52%, M. stramineus 15%, R. sanguineus 3%, G. gallinae 2%, G. gigas 2%, and D. gallinae 2%. Data were analyzed using Chi Square and Correspondence. The results of infestation was higher in males than females, although the difference was not statistically significant (P>0.05). Results of analysis of the pattern of investation of ectoparasites in the region that includes in males and females body are M. gallinae in the region of the back. L. caponis in the region of the wings. M. stramineus. R. sanguineus in the region of the head-neck, G. gigas in the region of belly, G. gallinae in the region of the leg just found in the males body. D. gallinae in the region of the leg just found in the females body.

Identification of Gastrointestinal Worms Egg on Spotted Deer (Axis axis) and Bawean Deer (Axis kuhlii) at Wonorejo Bibit Park and Surabaya Flora Park Achmad Hasan Sahani; Sri Mulyati; Tri Bhawono Dadi; Sri Mumpuni Sosiawati; Kusnoto Kusnoto; Ratna Damayanti
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to identify the type of gastrointestinal worms egg of Spotted Deer (Axis axis) and Bawean Deer (Axis kuhlii) at Wonorejo Bibit Park and Surabaya Floral Park. The research was conducted in October-December 2017 used total 50 specimen of sample faeces, 40 sample faeces from Surabaya Flora Park and 10 sample faeces from Wonorejo Bibit Park, the samples analyzed at the Department of Parasitology Veterinary, Faculty of Veterinary Medicine, Airlangga University. The results showed that the degree of helmint gastrointestinal in Bawean Deer and Spoted Deer at Wonorejo Bibit Park showed 0% while at the Surabaya Flora Park is 15%. The type of worm found were Trematoda class it was Fasciola sp. The resuls of the analysis used Chi-Square test and showed no significantly differences degree of gastrointestinal helminth infection (p>0.05) .

Co-Authors Abdul Samik Achmad Hasan Sahani Agnes Theresia Soelih Estoepangestie Akbar Wijaya Putra Purnama Aksono HP., Eduardus Bimo Andhika Yudhantama Subroto Andriani Dwi Siswarini Arimbi Arimbi Arum Puspitasari Bambang Sektiari Lukiswanto Bernideta Dewi Kriswijayanti Boedi Setiawan Bryan Ahmad Affan Lubis Budiarto Budiarto Candra Dwi Atma Chusniati, Sri Cintia Larasati Dhio Asmaydo Didik Handijatno Diyah Ayu Candra E Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Ellza Agatha Damayanti Endang Suprihati Eny Coolfina Simarmata Era Insivitawati Erma Safitri Fania Selfiannisa Firman Hadi Fanani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hari Suprapto Hartono Hartono Hemasayu Nirmala Putri Herry Agoes Hermadi Hervina Benazir Ardiyanti Ine Karni Iwan Sahrial Hamid Jihaan Haajidah khoirul - Arifin Kismiyati , Koesnoto Supriandono Kuncoro Puguh Santoso Lianny Nangoi Lucia Tri Suwanti, Lucia Tri Mas’ud Hariadi Miftahul Jannah Mochamad Lazuardi moh. arief ma'ruf Mohammad Sukmanadi Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Ngakan Made Rai Widjaja Nisa’ Rachmaningtyas Putri Nove Hidayati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Ogen Sea Poedji Hastutiek Primarizky, Hardany Rahmahani, Jola Rahmi Sugihartuti Ratna Damayanti Retno Bijanti Retno Bijanti Ria Nikmatul Jannah Sardjana, I Komang Wiarsa Sesa Puput Febriyanti Setiawan Koesdarto Setiawati Sigit Soeharsono Soeharsono Sri Mulyati Sri Mumpuni Sosiawati Sri Subekti Suherni Susilowati Sunarso, Agus Talita Yuanda Reksa Titom Gusmana Putra Perdana Tri Bhawono Dadi Tutik Juniastuti Tyasningsih, Wiwiek Vanna Lidya Kharisma Vindo Rossy Pertiwi Warda Nafalizza Efendi

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