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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Journal of Tropical Soils Paediatrica Indonesiana Health Notions Journal of Fisheries & Marine

. Suwarno

Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 3288572

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Articles

Determinan Antigen Gen omp2a Brucella abortus Isolat Lokal Ratih Ratnasari; Didik Handijatno; . Suwarno; Fedik Abdul Rantam
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : IPB University

Penyakit Brucellosis pada sapi disebabkan oleh Brucella abortus dan dikenal sebagai penyakitreproduksi menular pada ternak. Brucellosis merupakan penyakit zoonosis karena dapat menularpada manusia. Penelitian ini bertujuan untuk mengetahui determinan antigen yang terdapat pada genomp2a B. abortus isolat lokal yang telah diblasting ke asam amino (protein). Sampel bakteri berasaldari sapi penderita Brucellosis asal Sulawesi Selatan dan Nusa Tenggara Timur. Gen omp2a diamplifikasimelalui tehnik Polymerase Chain Reaction (PCR) dengan menggunakan primer 2ab5F dan 2a900R.Produk PCR disekuensing untuk mendapatkan sekuen nukleotida gen omp2a B. abortus isolat lokal.Sekuen nukleotida ini dianalisis tingkat homologinya terhadap isolat asal mancanegara yang diaksesdari GenBank dengan menggunakan BLAST. Sekuen nukleotida gen omp2a diblasting ke asam aminokemudian dengan metode Kolaskar dan Tongaonkar antigenicity dapat diperoleh determinan antigenpada antigen protein membran luar (OMP) B. abortus isolat lokal. Hasil menunjukkan bahwa tingkathomologi antara sekuen gen omp2a B. abortus isolat lokal dengan isolat asal mancanegara mempunyaitingkat homologi yang tinggi (99% - 100%). Hasil prediksi determinan antigen didapatkan enamdeterminan antigen pada antigen OMP B. abortus isolat lokal.

Expression of Catalase and Malondialdehyde Levels in Silicon Dioxyde-exposed Lung Tissue of Mice Treated with Moringa oleifera Leaves Extract Kusmiyati Kusmiyati; Soedjajadi Keman; Muhammad Amin; Suwarno Suwarno
Health Notions Vol 2, No 3 (2018): March
Publisher : Humanistic Network for Science and Technology (HNST)

Silica particle such as silicon dioxide (SiO2), is considered as a hazardous and cytotoxic particle. Silica particle exposure leads to oxidative stress in lung tissue. Moringa oleifera is a plant with potential antioxidant compounds. Therefore the aim of this study was to analyze the effect of M. oleifera leaves extract (MLE) on expression of catalase enzyme and malondialdehyde (MDA) levels in lung tissue of mice exposed to silica particles. This study was an experimental study with randomized posttest-only control group design using 30 male Balb/c strain mice, 8-10 weeks of age, 20-30 g body weight (BW), which were randomly divided into five groups. Group 1 was the negative control group, group 2 was exposed to SiO2 particle and set as the positive control group, group 3 was treated with MLE 2 mg/20 g BW, group 4 was treated with MLE 5 mg/20 g BW, and group 5 was treated with MLE 8 mg/20 g BW. After 90 days, mice were sacrificed by cervical dislocation and the lung tissues were examined. Study results showed that expression of catalase in lung tissues of MLE-treated group was higher than that of positive control group, but not statistically significant. There was a significant difference of MDA level in lung tissue among groups. MDA level of groups treated with MLE 2 mg/20 g BW and 5 mg/20 g BW was lower than that of control group, while group treated with MLE 8 mg/20 g BW showed higher MDA level than control group (One Way ANOVA, p<0.05). It is concluded that administration of MLE indicates to prevent SiO2 inducedoxidative stress in lung tissue of Balb/c mice.Keywords: Antioxidant, Catalase, Malondialdehyde, Moringa oleifera, Silicon dioxyde

Deteksi Molekuler Gen Penyandi Protein Virb11 pada Brucella abortus Isolat Lokal Asal Pinrang, NTT dan Strain Vaksin Maria Gladis Bupu Maze; Didik Handijatno; Wiwik Tyasningsih; Suwarno Suwarno; Agnes Theresia Soelih Estoepangestie; Rahaju Ernawati
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Brucellosis in cattle is a disease caused by Brucella abortus due to the reduction in livestock population caused by abortion, stillbirth, weak birth, infertility and sterility. Brucella abortus has several potentialvirulence factors, i.e. virB11 gene that encodes VirB11 protein is an important virulence factor acts as an ATPase for assembling organelles when the bacteria replicate, helping to complete the bacterial cycle and agress to another cells. The aim of this study are to re-identification Brucella abortus and detect virB11 gene as encoding of B. abortus VirB11 protein in local isolates from Pinrang, NTT, strain vaccines S19 and RB51. The isolates Brucella abortus were re-cultured in Brucella agar base and re-identification is followed by microscopic with Gram staining and biochemical tested with urease, citrat, indol and TSIA test. virB11gene was detected with PCR method. The PCR result showed virB11 gene have DNA band 720 bp.virB11 gene are present in local isolates from Pinrang, NTT, strain vaccines S19 and RB51.

Analytical study of inflammatory cytokines and immunoglobulin expression following dust mite allergen exposure in pregnant mice Risa Etika; Subijanto Marto Sudarmo; Suwarno Suwarno; Muhammad Pradhika Mapindra; Muhammad Pradhiki Mahindra
Paediatrica Indonesiana Vol 61 No 6 (2021): November 2021
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/pi61.6.2021.336-42

Background Allergen tolerability due to allergic immune reactions could be transferred through the placenta from maternal to fetal circulation. Hence, a further investigation regarding the tolerability following mite allergen exposures is desirable. Objective To evaluate various doses of mite allergens and cytokines associated with Th1, Th2, and Treg cells with regards to possible allergic tolerance in neonatal mice. Methods This study used an experimental design with a post-test only control group, to assess the effect of mite allergens on pregnant BALB/C mice and their newborns. In this study female BALB/C mice aged 10 weeks were mated with male mice, then pregnant BALB/C mice were exposed to allergens at 4 weeks gestation. During pregnancy, pregnant femalesâ€™ blood specimens were taken to measure cytokines and immunoglobulins. Meanwhile, neonatal blood specimens were taken at 2 weeks postnatally to measure cytokines and immunoglobulins. Blood specimens from pregnant BALB/C mice and their newborns were evaluated using ELISA kits for the following cytokines: interleukin (IL)-2, interferon (IFN)-Î³, interleukin (IL)-4, IL-5, IL-10, TGF-Î²1, as well as immunoglobulins (Ig)G-1, IgG-2a, IgG-2b, IgG3 subclass, IgM, IgA, and IgE. The case group was the group that received high and low doses of exposure, while the control group did not get exposure. Results In response to low dose mite allergen exposure, there were significant increases of IL-2, IFN-y, and IL-4, IL-5, and TGF-Î²1 in mothers and neonates. Pregnant mices that received high doses of allergens, however, had significant increases in IL-5 and TGF-B1; results were likewise for their offspring. Mothers and neonates, had significantly increased expression of IgG subclasses after a low dose of dust mite allergen. Following a ten-fold increase in allergen dose, the mothers showed significant increases in IgA, IgM, IgE, and IgG subclasses, whereas in neonatal mice, those immunoglobulin levels were not significantly different from control mice. Conclusion Exposure to mite allergens can trigger regulatory functions of Th1, Th2, and Tregs cells to activate their cytokines, except IL-10. The regulatory function of Tregs is dominated by TGFÎ² in maternal and neonatal mice, at low and high doses. Th1 cytokines express cytokines during exposure only to low-dose allergens and Th2 cells regulate IL-5 levels to both low- and high-dose allergens.

Deteksi Koi Herpesvirus (KHV) pada Ikan Nila (Oreochromis niloticus) yang Diinfeksi secara Buatan [ Detection of Koi Herpesvirus (KHV) in Nile Tilapia (Oreochromis niloticus) Infected by Artificially Infection ] Hari Suprapto; Suwarno Suwarno; Muhammad Sungging Pradana
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i1.11230

Abstract Koi Herpesvirus (KHV) was formerly known as Cyprinid herpesvirus-3 (CyHV-3) and carp nephritis interstitial and necrosis gill virus (CNGV) is a virus that infects common carp and koi (Cyprinus carpio and C. carpio koi) in farmed and wild population. KHV cause disease at a temperature of 18-25 °C with mortality rate of 80-90 % in fry and adult fish. Currently KHV also detected in tilapia from the results of monitoring in the field. The presence of KHV in tilapia can occur as a result of maintenance in cages adjacent to the infected carp. KHV diagnostic method currently based on case definition and PCR (Polymerase Chain Reaction) detection. The basic concept of PCR is one DNA molecule is used to produce two copies, then four, then eight and so forth through multiplication by polymerase. PCR results sometimes indicated the presence of a faint band caused a low amount of virus, so it is necessary to investigate the presence of KHV DNA in tilapia using different doses of infection. This study aimed to determine the KHV infectivity in nile tilapia were infected by artificially infection and determine dose KHV infection that can infect nile tilapia. The study design used true experimental with with the presentation of descriptive data. Dose of viral infection are 1 ID50, 10 ID50, 100 ID50 and 1000 ID50. The results showed that no clinical symptoms of KHV infected in nile tilapia. The results of electrophoresis of PCR products showed that the mucus of nile tilapia were infected with a 1000 ID50 immersion dose showed thin bands. The same results are also shown in the gill of nile tilapia infected by gill spray at 1000 ID50 dose. Fish were infected by injection, KHV was not detected in mucus, gill, kidney and liver. The results above show nile tilapia cannot be infected by KHV on various treatment

Identifikasi Koi Herpesvirus pada Dosis yang Berbeda dengan Metode Imunohistokimia Streptavidin Biotin pada Ikan Nila (Oreochromis niloticus) [Identification of Koi Herpes Virus At Different Dose with Streptavidin Biotin Methods Immunohistochemistry on Tilapia (Oreochromis niloticus)] Hari Suprapto; Faricha Risma Nurani; Suwarno Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11277

Abstract KHV is a viral disease in goldfish and Koi Fish (Cyprinus carpio) is a highly contagious, infecting all ages or sizes of fish and aquaculture systems. This disease results in mortality between 80% - 100% of the total fish population, with an incubation period of between 1-7 days. KHV infection triggered by a drop in ambient temperature so it is referred to as a virus that attacks when cold (a cold virus). The disease attacks the temperature range of 18-28 ° C and can cause death. This virus attacks can occur at any age of the fish ranging in size from the seed to the parent. The most prominent clinical symptoms due to KHV infection is the sudden death 1-2 days after infection. Other clinical symptoms are necrosis of the gills, sunken eyes, bleeding at the gills, hemorraghe, excess mucus production in the body, and secondary bacterial infections or parasitic infestations. Streptavidin biotin immunohistochemical method was applied to the study of cells and tissues by staining imunostaining. Technique of determining the existence of (location) antigen (target protein) in tissue using antigen-antibody reaction that begins with histotenik procedure is the procedure of making tissue sections (histology). This method has high sensitivity and fast so that it can be applied to the detection of KHV antigen in tissues of fish Tilapia (Orechromis niloticus). This study aims to determine the presence of KHV antigen in tilapia gills after infection. The method used in this study is the experimental method. Dose of virus titer used was 1 ID50, ID50 10, 100 ID50, ID50 1000. The results showed that the Streptavidin Biotin immunohistochemical examination imunopatologi able to detect KHV virus Ag in the gill tissue of tilapia (Oreochromis niloticus) were infected with different doses. In all treatments showed on all the gills are KHV antigen is indicated by the presence of a golden brown color on the gill epithelium. Advised Streptavidin biotin immunohistochemistry test was applied to the detection of the presence or absence of fish-carp KHV carrier as a routine control program and control, including the prevention and outbreak of KHV in Indonesia, because of KHV were attacked in tilapia was persistent and did not show clinical symptoms

Quality Improvement of Compost from Empty Oil Palm Fruit Bunch by the Addition of Boiler Ash and its effect on Chemical Properties of Ultisols and the Production of Mustard (Brassica juncea L.) Mulyani, Sri; Suryaningtyas, Dyah Tjahyandari; Suwardi, .; Suwarno, .
JOURNAL OF TROPICAL SOILS Vol. 21 No. 3: September 2016
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2016.v21i3.161-169

Productions of crude palm oil (CPO) produce waste which include the empty oil palm fruit bunch (EOPFB), palm oil mill effluent, shells, and fiber. The combustions of shell and fiber as boiler feed produce waste in the form of boiler ash. Boiler ash is very potential to use as an additive to improve quality of the EOPFB compost. The objectives of this research were to study : 1) effect of boiler ash on the quality of the EOPFB compost, 2) effect of EOPFB compost on the chemical properties of Ultisol, and the yield of mustard. The first experiment was about quality improvement of compost from EOPFB by the addition of boiler ash. The treatments applied were four dose levels of boiler ash: K1= 0%, K2 = 15%, K3 = 25%, and K4 = 35%. The second experiment was greenhouse pot experiment. This experiment was consisted of thirteen treatments with three replications arranged in completely randomized design (CRD). The treatments applied were: four types of compost from the first experimental results aplied to the soil with four dose levels, i.e : 0, 10, 20 and 30 Mg ha-1. The results showed that the addition of boiler ash at the beginning of the composting process improved the quality of the EOPFB compost: which increased pH, amount of humic acids, macro and micro nutrients content and decreased content of Pb. The aplication of all compost –K1, K2, K3, K4– to Ultisol increased pH H2O, P2O5, organic-C, total-N, exchangeable-Ca, exchangeable-K and yield of mustard and decreased exchangeable-H, exchangeable-Al. Moreover the yield of mustard was increased about 84% (33.9 g plant-1) with K4 at doses 20 Mg ha-1 and 85% (34.1 g plant-1) with K3 at doses 10 Mg ha-1.

Co-Authors . Suwardi Agnes Theresia Soelih Estoepangestie Cutrisni, . Didik Handijatno Dyah Tjahyandari Suryaningtyas Faiza Chairani Suwarno Faricha Risma Nurani Fedik Abdul Rantam Feni Shintarika Hari Suprapto Kusmiyati Kusmiyati Maria Gladis Bupu Maze Muhammad Amin Muhammad Pradhika Mapindra Muhammad Pradhiki Mahindra Muhammad Sungging Pradana Rahaju Ernawati Ratih Ratnasari Risa Etika, Risa Soedjajadi Keman Sri Mulyani Sudarmo, Subijanto Marto Wiwik Tyasningsih

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