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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Aceh Journal of Animal Science Vitek : Bidang Kedokteran Hewan Journal of halal product and research (JHPR) journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Journal of Fisheries & Marine
Didik Handijatno
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
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Determinan Antigen Gen omp2a Brucella abortus Isolat Lokal Ratih Ratnasari; Didik Handijatno; . Suwarno; Fedik Abdul Rantam
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.347 KB) | DOI: 10.29244/avi.2.1.17-25

Abstract

Penyakit Brucellosis pada sapi disebabkan oleh Brucella abortus dan dikenal sebagai penyakitreproduksi menular pada ternak. Brucellosis merupakan penyakit zoonosis karena dapat menularpada manusia. Penelitian ini bertujuan untuk mengetahui determinan antigen yang terdapat pada genomp2a B. abortus isolat lokal yang telah diblasting ke asam amino (protein). Sampel bakteri berasaldari sapi penderita Brucellosis asal Sulawesi Selatan dan Nusa Tenggara Timur. Gen omp2a diamplifikasimelalui tehnik Polymerase Chain Reaction (PCR) dengan menggunakan primer 2ab5F dan 2a900R.Produk PCR disekuensing untuk mendapatkan sekuen nukleotida gen omp2a B. abortus isolat lokal.Sekuen nukleotida ini dianalisis tingkat homologinya terhadap isolat asal mancanegara yang diaksesdari GenBank dengan menggunakan BLAST. Sekuen nukleotida gen omp2a diblasting ke asam aminokemudian dengan metode Kolaskar dan Tongaonkar antigenicity dapat diperoleh determinan antigenpada antigen protein membran luar (OMP) B. abortus isolat lokal. Hasil menunjukkan bahwa tingkathomologi antara sekuen gen omp2a B. abortus isolat lokal dengan isolat asal mancanegara mempunyaitingkat homologi yang tinggi (99% - 100%). Hasil prediksi determinan antigen didapatkan enamdeterminan antigen pada antigen OMP B. abortus isolat lokal.
SA-11 The Total Count of CPS on Hand Holding Dog Swab in UNAIR Animal Hospital, Before and After Cleaning based on Islamic Rules and Cleaning Using Soap Gretania Residiwati; Mustofa Helmi Effendi; Didik Handijatno
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.112 KB)

Abstract

Purify our self from the dirt is highly regarded in Islamic principles because that is one of legitimate requirements of a worship. In Islamic law, dogs are classified including into the severe unclean category, where there are some perspectives against the nature of unclean possess by dog.  The way of purification is to wash as much seven times, and one of them using soil (Handi, 2008).Essentially, the unclean determination for dog saliva is viewed from ritual dimensions, instead of rational, thus there should be no reason logically. Furthermore, we cannot mix the concept of holiness according to the religion with a sterile concept if we relate to the medical side. However, as far as we know the sacred way, surely there is a great secret that Our Creator has saved, thus we need to explore more deeply.The means of transmitting pathogenic germs has been realized since the 1840s, where proper hand hygiene can reduce or prevent the occurrence of nosocomial infections. Dobson (2003) mentioned that washing hands can prevent more than 1 million deaths per year due to illness diarrhea, while washing hands with soap can reduce diarrhea up to 47%.Jayne (2002) who compared the number of bacteria that successfully grown from the saliva of dogs, cats and humans, mentioned that bacteria in dog saliva had the largest number with 53 colonies, while cats had 16 and human with 5 colonies. The bacteria derived from dog saliva have the fastest grow and the most varied colonies colors.Staphylococcus sp. is a normal flora that we can find on the skin, ears, swabs of nasal mucosa and mouth, also saliva of dog. This bacterium includes into opportunistic pathogens bacteria that can cause canine pyoderma, abscesses, otitis externa, infection of wound and urinary tract in dogs. In humans, they can cause external otitis, cardiomyopathy and endocarditis, food poisoning outbreaks, catheterrelated bacteremia, pneumonia and brain abscesses (Borjesson, 2014). S.intermedius and S.aureus are bacteria belonging to the Coagulase Positive Staphylococci (CPS) group which plays an important role in cases of skin infections in dogs and they are zoonotic (Hajek, 1967). Tanner et al (2000), reported that this bacterial transmission through pets to humans is a common case at home and inflict various diseases on dog owners.In Indonesia, limited studies of various causative agents of diseases makes we lack to know whether the cause of the various diseases that are currently emerging. Thus, the efforts of preventive that should be more necessary to do rarely get a serious attention. Whereas very possible, that our lovely pet is one of the source of the various diseases transmissions because of less precise of biosafety.There is no further proof yet, whether the concept of Islamic purification for saliva of dog also includes the concept of sterility against bacteria based on medical guidance. As an early stage, this paper will be elaborated how the total of CPS from hand swab holder dogs at Animal Hospital of Education, Airlangga University, Surabaya, before and after purification with soil according to Islamic principles and cleaning using soap.
Molecular characterization of Pasteurella multocida pfhaB1 gene fragment from buffalo and cattle isolates from Nusa Tenggara Timur Indonesia Ine Karni; Didik Handijatno; Lucia Tri Suwanti; Kusnoto Kusnoto; Jola Rahmahani; Wiwiek Tyasningsih
Aceh Journal of Animal Science Vol 4, No 2: December 2019
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.4.2.13581

Abstract

Almost all regions in Nusa Tenggara Timur East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Haemorragic Septicaemia (HS), which is caused by Pasteurella multocida  (P. multocida ) Serotypes B: 2. The fragment  pfhaB1gene is one of the virulence factors of P. multocida.The objective of this study was to determaine the phylogenetic, homology of P. multocidapfhaB1gene fragment of isolatedfrom Buffalo and Cattle in NTT. The P. multocida isolateswere re-cultured and further microscopic examined the biochemical tests, PCR, sequencing, homology, and phylogenetic relatedness test. P. multocida was observed as gram negative, coccobacilus, no growth on MacConkey Agar, does not produce H2S and gas, nonmotile and indole positive, does not produce urease enzymes, does not use citrate as a carbon source, does not ferment maltose and lactose but it does ferment glucose, sucrose and mannitol. ThepfhaB1gene fragmentfrom buffalo and cattle NTT isolates and also Katha strain vaccine showed DNA band 506 bp. P.multocida isolates from buffalo and cattle in NTT have 91% - 99% score homology with the comparative isolate. The isolate P. multocida from buffalo and cattle in NTT are in one cluster and their phylogenetic relatedness is close to isolates from Iran and India. It is concluded that the  pfhaB1gene fragmentof P. multocida from buffalo and cattle isolates have phylogenetic relatedness close and homolog with the other comparative isolates.    Keywords: Haemorrhagic Septicaemia; Nusa Tenggara Timur; Pasteurella multocida;  pfhaB1gene
Profile Protein "Brucella Abortus" Local Isolate with SDS Page Method Desty Apritya; Didik Handijatno; Ratih Ratnasari
VITEK : Bidang Kedokteran Hewan Vol 4 (2014): VITEK - Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this research was to find out the profile protein Brucella abortus local isolate. The samples bacteries were obtained from Balai Besar Veteriner Maros, South Sulawesi. Whole protein was obtained from Brucella abortus local isolate by ultrasonic homogenizer. The protein profile was analyzed by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) with 4% stacking gel, 12% separating gel and standard of molecular weight was 10 – 200 kDa.The results of this research showed that the profile protein had seven fractions there were 112 kDa, 72 kDa, 54 kDa, 46 kDa, 41 kDa, 31 kDa and 20 kDa.
Total plate count of commercial pasteurized milk sold by street vendors in Mulyorejo sub-district Surabaya Lailatul Maghfiroh; A. T. Soelih Estoepangestie; Tri Nurhajati; Nenny Harijani; Mustofa Helmi Effendi; Didik Handijatno
Journal of halal product and research (JPHR) Vol. 4 No. 2 (2021): Journal of Halal Product and Research (JHPR)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jhpr.vol.4-issue.2.65-70

Abstract

Food and nutritional quality of society is one of mine focuses of the government. Milk have complete nutrients but also can be the source food borne disease. The Veterinary Public Health itself has the responsibility to provide guarantees of food safety from animal resources which is called ASUH (safe, healthy, wholesome and halal) to be consumed by society. This study aimed to know the Total Plate Count of commercial pasteurized milk sold by street vendors in Mulyorejo Sub-district Surabaya. The study was conducted from January until February 2018 at the Laboratory of Veterinary Public Health Department, Faculty of Veterinary Medicine, Universitas Airlangga. Thirty samples of commercial pasteurized milk were bought from three street vendors then examined using Pour Plate Method of Total Plate Count. Total Plate Count result showed that the number of bacteria in commercial pasteurized milk sold by street vendors in Mulyorejo Sub-district Surabaya was 1.0 x 104 CFU/ml, that met the National Standard of Indonesia (SNI) 01-3951-1995.
CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Ratih Novita Praja; Didik Handijatno; Setiawan Koesdarto; Aditya Yudhana
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.411 KB) | DOI: 10.21157/j.ked.hewan.v12i1.8091

Abstract

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.
Uji Potensi Antifungi Perasan Daun Seledri (Apium graveolens L) Terhadap Aspergilllus terreus Secara In Vitro [Antifungal Potential Test Of Celery Leaves Juice (Apium graveolens L) Against Aspergilllus terreus By In Vitro] Sudarno Sudarno; Illa Rohdiana Hermawati; Didik Handijatno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11379

Abstract

Abstract Aspergillus terreus is a fungal that is able to produce several mycotoxins such as patulin, sitrinin and aflatoxin and contained in processed mackerel mixed with fresh steamed way. The impact of the use of chemicals to control fungal attack A. terreus showed a negative effect. This study aims to determine the potential and the minimum concentration of the juice of the leaves of celery (Apium graveolens L) as antifungal against the growth of A. terreus in vitro. The experiment was conducted at the Laboratory of the Faculty of Fisheries and Marine Airlangga University in July 2013. The method was used in this research that using paper disc diffusion method. Treatment outcome data were analyzed using descriptive statistics. Test potential antifungal celery juice in inhibiting the growth of fungus A. terreus obtained negative be results. The concentrations of juices were given as a treatment of 10 % to 100 % are not able to inhibit the growth of A. terreus shown with no inhibition zone formation in all treatments except the concentration of 10 % formalin as a control (+).
MOLECULAR IDENTIFICATION OF SARCOPTES SCABIEI VAR. CUNICULI FROM SURABAYA AND MALANG REGIONS OF EAST JAVA Desiandura, Kurnia; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Handijatno, Didik
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 6 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (752.607 KB) | DOI: 10.20473/ijtid.v6i6.5436

Abstract

Scabies is a zoonotic skin disease caused by Sarcoptes scabiei mites. As an emerging/re-emerging parasitic disease, scabies represents a significant global threat to both human and animal health. Numerous cases of scabies in Indonesia have been reported, which support research on the prevalence of S. scabiei. However, most such studies have involved conventional morphological studies, with limited molecular diagnostic studies. The purpose of the present study was the genetic characterization of S. scabiei var. cuniculi in domestic rabbits to generate baseline genotypic data. S. scabiei var. cuniculi was isolated and identified from scabies-infected rabbits from the Surabaya and Malang regions of East Java. Molecular identification was performed using Polymerase Chain Reaction (PCR) using specific primers targeting the COX1 gene. We performed COX1 PCR using rabbit isolates of S. scabiei from Indonesia. To the best of our knowledge, no such study had been reported previously. This study was performed in the Laboratory of Veterinary Parasitology, Faculty of Veterinary Medicine and the Tropical Disease Diagnostic Center Laboratory, Universitas Airlangga. The results with agarose gel electrophoresis revealed a 289 bp PCR product amplified from the DNA of S. scabiei isolates from both Surabaya and Malang in accordance with the expected COX1 amplicon size, that indicated a single band 289 bp in length, demonstrating specific detection of S. scabiei var. cuniculi from Surabaya and Malang using COX1 primers. The results were consistent with the calculated amplicon size based on primer positions within the COX1 locus, with the forward primer spanning nucleotides 61–94, and the reverse primer spanning nucleotides 331–350 ( 350 − 61 = 289 bp).  PCR genotyping of the isolates yielded an identical nucleotide length of 289 bp. Further studies are required to sequence the amplified fragments for homology assessment.
Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Mutiarasari, Nonie Olivia Adia; Harijani, Nenny; Rantam, Fedik Abdul; Raharjo, Dadik; Estoepangestie, Agnes Theresia Soelih; Handijatno, Didik
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Exploration of the Antibacterial Potential from Rice Eel Skin Mucus (Monopterus albus) Against Bacteria Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus Praja, Ratih Novita; Yudhana, Aditya; Handijatno, Didik; Al-Madinah, Wardatul Qoryah; Hamonangan, Jonathan Mark; Insani, Alivia Khairina; Prameswari, Nindya Pradnya; Prasetyo, Dhenatra Rifqy; Praja, Shifa Salsabilla
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.197-205

Abstract

The skin mucus of the rice eel (Monopterus albus) contains various antibacterial compounds and has the potential as a synthetic antibiotic. This research was conducted to explore the potential antibacterial power of the rice eel skin mucus against some pathogenic bacteria in freshwater fish. The bacteria were isolated from five samples of rice eel cultivation ponds belonging to Mr. Sabwan and then challenged with the mucus of the eel's skin through diffusion tests using paper discs. The rice eel skin mucus tested its antibacterial activity against three species of freshwater bacteria, Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus, by testing sensitivity and inhibitory zones. The inhibitory zones of each bacterium were measured using Vernier caliper which refers to the standardization of the inhibitory zone: < 4 mm no activity, 5-9 mm weak, 10- 14 mm medium, and > 15 mm strong. Test results showed that the eels' skin mucus could inhibit the growth of bacteria Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus.
Co-Authors . Suwarno A. T. Soelih Estoepangestie Adikara, Tatang Santanu Agnes Theresia Soelih Estoepangestie Al-Madinah, Wardatul Qoryah Ayang Mahindra Christian Marco Hadi Nugroho Desiandura, Kurnia Desty Apritya Elisa Herina Dimariwu Emmanuel Djoko Poetranto Endang Suprihati Fedik Abdul Rantam Hamonangan, Jonathan Mark Hani Plumeriastuti Illa Rohdiana Hermawati Ine Karni Insani, Alivia Khairina Kusnoto Kusnoto Lailatul Maghfiroh Lucia Tri Suwanti, Lucia Tri Maria Gladis Bupu Maze Mufasirin Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nenny Harijani Nunuk Dyah Retno Lastuti Praja, Ratih Novita Praja, Shifa Salsabilla Prameswari, Nindya Pradnya Prasetyo, Dhenatra Rifqy Prestalia Dwi Rachmawati Rahaju Ernawati Raharjo, Dadik Rahmahani, Jola Ratih Ratnasari Ratih Ratnasari Residiwati, Gretania Rima Ratnanggana Prasetya Romy Muhammad Dary Mufa Setiawan Koesdarto Sudarno, Sudarno Tri Nurhajati Tyasningsih, Wiwiek Wiwik Tyasningsih Yudhana, Aditya