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All Journal Jurnal Veteriner Journal of Parasite Science
Agnes Theresia Soelih Estoepangestie
Department Of Veterinary Public Health - Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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Journal : Jurnal Veteriner

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Deteksi Molekuler Gen Penyandi Protein Virb11 pada Brucella abortus Isolat Lokal Asal Pinrang, NTT dan Strain Vaksin Maria Gladis Bupu Maze; Didik Handijatno; Wiwik Tyasningsih; Suwarno Suwarno; Agnes Theresia Soelih Estoepangestie; Rahaju Ernawati
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.177 KB) | DOI: 10.19087/jveteriner.2020.21.4.503

Abstract

Brucellosis in cattle is a disease caused by Brucella abortus due to the reduction in livestock population caused by abortion, stillbirth, weak birth, infertility and sterility. Brucella abortus has several potentialvirulence factors, i.e. virB11 gene that encodes VirB11 protein is an important virulence factor acts as an ATPase for assembling organelles when the bacteria replicate, helping to complete the bacterial cycle and agress to another cells. The aim of this study are to re-identification Brucella abortus and detect virB11 gene as encoding of B. abortus VirB11 protein in local isolates from Pinrang, NTT, strain vaccines S19 and RB51. The isolates Brucella abortus were re-cultured in Brucella agar base and re-identification is followed by microscopic with Gram staining and biochemical tested with urease, citrat, indol and TSIA test. virB11gene was detected with PCR method. The PCR result showed virB11 gene have DNA band 720 bp.virB11 gene are present in local isolates from Pinrang, NTT, strain vaccines S19 and RB51.
Co-Authors . Suwarno Didik Handijatno Ellza Agatha Damayanti Endang Suprihati Kusnoto Kusnoto Maria Gladis Bupu Maze Nunuk Dyah Retno Lastuti Poedji Hastutiek Rahaju Ernawati Wiwik Tyasningsih