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All Journal Journal of the Indonesian Tropical Animal Agriculture Majalah Obat Tradisional Tropical Animal Science Journal
R. Murwani
Faculty of Animal Sciences, Diponegoro University
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy Health Professions Medicine & Pharmacology Public Health

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AKTIVITAS ANTI OKSIDAN DAN ANTI TUMOR KANDUNGAN TANAMAN HERBAL INDONESIA L. D., Mahfudz; Murwani, R.; T. H., Kusumowardani; Hou, D. X.
Majalah Obat Tradisional Vol 16, No 2 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.038 KB) | DOI: 10.14499/mot-TradMedJ16iss2pp68-74

Abstract

Penelitian ini ditujukan untuk mengevaluasi dan membandingkan aktivitas antioksidan 2 jenis herbal Indonesia, melalui meneliti kandungan fenol, cytotoksid dan induksi apoptosis terhadap sel HL-60. Data ini merupakan informasi yang berguna untuk manusia dalam menciptakan makanan kesehatan dan aplikasi baru dari antioksidan alami dalam bahan makanan sebagai pangan fungsional dan sebagai bahan untuk obat kanker. Penelitian ini menggunakan 2 buah sampel A dan sampel B. Aktivitas antioksidan total di analisa dengan metode DPPH. Kandungan total fenol di tentukan dengan metode Folin-Ciocalteu dan dihitung sebagai ekuvalensi dari asam gallic dan di ekspresikan sebagai gallic acid equivalent (GAE) dalam mg/g sampel. Pengaruh ekstrak herbal terhadap cytotoksid terhadap pertembuhan sel promyelocytic leukimia (HL-60) dianalisa dengan MTT kalorimeter. Hasil ditampilkan sebagai rata-rata ± penyimpangan baku. Analisis Statistik menggunakan Student t-test, pada tingkat signifikansi p<0,05. Koefisien korelasi (r2) terhadap parameter yang di test di hitung menggunakan analisa regresi. Kandungan ekstrak sampel A kering adalah 242,4mg dan sampel B adalah 256,4mg. Diketahui bahwa efek memakan dari ekstrak herbal Indonesia terhadap radikal DPPH meningkat 0,3 – 1,5mg/ml, dimana sampel A adalah 14,3 - 64,29% dan sampel B adalah 9,09 - 57,53%. Total fenol tertinggi didapat pada sampel A (21,72mg GAE/g) dan kandungan fenol rendah pada sampel B (17,53 GAE/g). Apoptosis dari sel HL-60 dilihat dari perubahan morpologi (kondensasi kromatin). Kondensasi kromatin secara spesifik dan fitur yang berbeda dari sel apoptosis, diketemukan pada sebagian besar sel perlakuan. Hasil ini menunjukan bahwa kematian sel diakibatkan oleh ekstrak herbal Indonesia.
Body weight, haematological indices and intestinal morphometric of broilers provided with diets containing formic acid, butyric acid or their blends Isroli, I.; Murwani, R.; Yudiarti, T.; Widiastuti, E.; Wahyuni, H. I.; Sartono, T. A.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 1 (2020): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.1.37-46

Abstract

The study investigated the influence of dietary incorporation of formic acid, butyric acid or their blends on growth rate, haematological indices and intestinal morphometric of broilers. A number of 240 Lohmann MB-202 chicks were pass rounded to four dietary groups, including CONT (chicks taking in basal feed with no additive), BTRT (chicks receiving basal feed with 0.03% butyric acid), FRMT (chicks receiving basal feed with 0.1% formic acid) and BTRT+FRMT (chicks receiving basal feed containing 0.03% butyric acid and 0.1% formic acid). Weight of chicks and intake were measured every week, while blood sample was collected at day 21 and 35. At day 35, birds were slaughtered and small intestinal segments and digesta were collected. At day 21, body weight was bigger (P<0.05) in BTRT+FRMT than in other birds. Feeding the blends of formic and butyric acids resulted in higher (P<0.05) value of mean corpuscular haemoglobin concentration (MCHC) at day 21. Feeding organic acids was associated with the lower (P<0.05) proportion of thrombocytes in broilers at day 35. Supplementation of butyric acid resulted in higher (P<0.05) serum albumin concentration at day 35. Butyric acid produced higher (P<0.05) levels of triglyceride as well as low-density lipoprotein (LDL) in the serum. The mixture of formic and butyric acids resulted in higher (P<0.05) titer of antibody against Newcastle disease vaccine (NDV) at day 21. Feeding formic acid decreased (P<0.05) ileal population of lactose negative enterobacteria at day 35. Overall, dietary supplementation of the blends of formic and butyric acids resulted in improved growth and immune defence of broiler chicks. 
ISOLATION OF FUNGI FROM THE GASTROINTESTINAL TRACT OF INDIGENOUS CHICKEN Yudiarti, T.; Yunianto B.I., V.D.; Murwani, R.; Kusdiyantini, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 2 (2012): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.2.115-120

Abstract

Gastrointestinal tract of chicken is a place in which many kinds of fungi can be found. The aim ofthe research was to isolate fungi from the gastrointestinal tract of the indigenous chicken (AyamKampung). The chicken samples were four days, one week and two months old and were sampled fromchicken farm located in Yogyakarta. Potato dextrose agar (PDA) medium was used to grow the fungi.Fifty pure isolates of fungi were found from three different ages, those were four days, one week andtwo months old chicken were 5, 10 and 35 isolates respectively. The largest number of isolate was foundin ileum, then followed by caecum, jejenum and duodenum. The fifty isolate of fungi belonged to sevenspecies, those were Aspergillus fumigatus, Aspergillus niger, Chrysonilia crassa, Mucor circinelloides,Mucor sp, Rhizopus oligosporus and Rhizopus oryzae.
Dietary Supplementation of Spirulina platensis as a Substitute for Antibiotics in Arab Chicken (Gallus turcicus) Hasna, N. A.; Widiastuti, E.; Agusetyaningsih, I.; Wulandari, E. C.; Murwani, R.; Yudiarti, T.; Sartono, T. A.; Sugiharto, S.; Wahyuni, H. I.
Tropical Animal Science Journal Vol. 47 No. 2 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.2.180

Abstract

This study was conducted to determine the effect of adding Spirulina platensis to replace zinc bacitracin on performance, egg quality, blood profile, total gut bacteria, and liver histopathology of local indigenous Arab chicken (Gallus turcicus). One hundred and eight 28-week-old laying hens were distributed randomly to three treatments: T0 (control diet), T1 (T0 + 1% S. platensis), and T2 (T0 + 0.04% zinc bacitracin). The treatment was applied for 49 days. S. platensis and zinc bacitracin decreased feed intake (p<0.05), but the egg mass had no significant effect; this provides a good improvement in feed conversion ratio (p<0.05). S. platensis helped maintain persistent egg production (p<0.05). S. platensis and zinc bacitracin provided the best results on haugh unit (p<0.05). S. platensis increased the yolk score (p<0.05). Zinc bacitracin decreased erythrocytes (p<0.05) but was not significantly different from S. platensis. S. platensis and zinc bacitracin increased mean corpuscular volume (MCV) (p<0.05). Blood chemical profile and total gut bacteria were not affected by the treatments. S. platensis was the best at maintaining liver’s health (p<0.05). This research concludes that S. platensis can efficiently optimize feed consumption, enhance performance, maintain egg quality, and protect the liver damage of Arab chicken. Therefore, S. platensis could be considered to replace the use of antibiotics.
Egg Production, Blood Profile, and Histopathology in Japanese Quail with Phytogenic Additives Murwani, R.; Anggraeni, R.; Muslih, M. H.; Yogantara, A. O.; Mulyono
Tropical Animal Science Journal Vol. 47 No. 3 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.3.321

Abstract

This study aimed to determine the effect of combining phytogenic Curcuma aeruginosa Roxb powder with C. xanthorizza standardized extract or with Anredera cordifolia leaf powder in an antibiotic-free diet on the egg production, red and white blood cell profile, fecal endoparasite, serum biochemistry, and intestinal and liver histopathology of Japanese quails. Four hundred eight-month-old Japanese quails were raised in 5-tier cages, randomly allocated into four treatments, namely: T0 (standard diet), T1 (standard diet plus 1% C. aeruginosa Roxb rhizome powder), T2 (standard diet plus 1% C. aeruginosa Roxb rhizome powder and C. aeruginosa extract (equal to 200 ppm standardized curcumin), and T3 (standard diet plus 1% C. aeruginosa Roxb powder and 1% A. cordifolia leaf powder). The data were analyzed using variance analysis (ANOVA). Duncan’s test was carried out at a 5% significant level when a significant effect was found. The results showed that weekly egg production was not affected by phytogenic addition, but egg production significantly increased on the last day of treatment (p<0.05). Combining 1% C. aeruginosa Roxb and 1% A. cordifolia significantly increased (p<0.05) erythrocytes, hemoglobin, hematocrit, leukocytes, lymphocytes, serum glucose, uric acid, creatinine, and AST. However, their values remained within the normal range of Japanese quails. No endoparasites were found in the fecal samples. The addition of phytogenic did not affect the height of intestinal villi and crypt depth (p>0.05). Interestingly, intestinal inflammation levels were reduced significantly in T1 and T2 compared to the control, while T3 was the same as the control (p<0.05). An elevated liver score was found (1 score higher) in T2 (p<0.05). This study suggests that phytogenic additives can help reduce normal intestinal inflammation (due to harsh intestinal environment) and improve the performance of laying Japanese quail, especially in the absence of endoparasites or infection.
Endoparasite and Microbial Burden in Quail Farm Systems: A Biosecurity and Water Quality Assessment in Central Java, Indonesia Murwani, R.; Dewi, B. P. A.; Anggraeni, R.; Mulyono, M.; Ambariyanto, A.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.71

Abstract

Quail farming is vital for smallholder farmers in Central Java, Indonesia; however, productivity often declines due to poor biosecurity, inadequate water quality, and parasitic infections. This study aims to examine biosecurity practices, water quality, and endoparasite loads in ten quail farms (L1-L10) located in Semarang City and nearby regencies. Data were collected through observations, interviews, water sampling, and analysis of quail droppings. Biosecurity scores (maximum 18) were categorized into three groups: Group-1 (scores 13-18), Group-2 (scores 7-12), and Group-3 (scores 1-6). Water quality was assessed based on pH, E. coli, and coliform counts using triplicate samples from each site. The biosecurity score showed that three farms are in Group-1 (L1, L7, L9), five are in Group-2, and two are in Group-3 (L8, L10). The water pH ranged from 4.8 to 8.0; the highest E. coli count was 19.33 CFU/mL (L9), and the highest Coliform count was 89.67 CFU/mL (L5). L1 had the highest parasite load, with Coccidia, Strongyloides, and Ascaridia sp. at 1000 oocysts/g (OPG), 100 OPG, and 33.33 OPG, respectively. Meanwhile, L7 showed no endoparasites or water contamination. Egg production ranged from 64.29% to 90.00%. Although no significant differences in endoparasite prevalence were found among biosecurity groups or water quality (p>0.05), Coliform levels differed significantly (p<0.05), with the highest levels in conventional well-water. This study revealed that only about one-third of smallholder quail farms in Semarang and its surrounding regencies adhere to proper biosecurity measures. It was also the first to document quail endoparasite burdens as well as E. coli and coliform contamination in drinking water. 
Co-Authors Agusetyaningsih, I. Ambariyanto, A. D. X. Hou, D. X. Dewi, B. P. A. E. Kusdiyantini E. Widiastuti H. I. Wahyuni Hasna, N. A. I. Isroli Kusumowardani T. H., Kusumowardani M. Mulyono Mahfudz L. D., Mahfudz Mulyono Muslih, M. H. R. Anggraeni S. Sugiharto Sartono, T. A. Turrini Yudiarti V.D. Yunianto B.I. Wulandari, E. C. Yogantara, A. O.