E. Widiastuti
Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang 50275

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Effect of decocted turmeric on performance, hematological parameters and carcass traits of broiler chickens Isroli, I.; Yudiarti, T.; Widiastuti, E.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.263-269

Abstract

This study aimed to investigate the effect of decocted turmeric on performance, haematological parameters and carcass traits of broilers. Broiler chicks at 11 days of age were allotted to five groups, including T0 (birds drinking 100% water), T1 (25% decocted turmeric + 75% water), T2 (50% decocted turmeric + 50% water), T3 (75% decocted turmeric + 25% water) and T4 (100% decocted turmeric). The study was arranged based on a completely randomized design (CRD), parameters investigated was performance and blood chemistry (leukocyte, total protein, albumin, globulin, uric acid, glucose). The data were subjected to analysis of variance at 5% probability. Decocted turmeric did not affect weight gain, feed and water consumption and feed conversion ratio of broilers. Lymphocytes proportion was higher (P<0.05) in T1, T2 and T4 than in T0 and T3 birds. The heterophils to lymphocytes ratio was lower (P<0.05) in birds receiving decocted turmeric than control. Globulin concentration and albumin to globulin ratio were higher and lower (P<0.05), respectively, in serum of T2, T3 and T4 than T0 and T1 birds. The proportion of heterophils and concentration of serum total protein, albumin, uric acid and glucose were not different among treatments. The weight of breast meat was higher and lower (P<0.05) in T3 and T4 birds, respectively, compared to the control. In conclusion, providing decocted turmeric through drinking water helped to improve stress responses and increased the weight of breast meat of broiler chickens. 
Effect of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chickens Sugiharto, S.; Yudiarti, T.; Isroli, I.; Widiastuti, E.; Wahyuni, H. I.; Sartono, T. A.; Nurwantoro, N.; Al-Baarri, A. N.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.286-294

Abstract

The objective of the present study was to evaluate the impact of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chicks. A number of 288 day-old-chicks (Lohmann MB-202 meat broilers) were distributed to four groups, including CONT (basal diet with no additive as a control), FORM (basal diet containing 0.1% formic acid), BUTR (basal diet with 0.03% butyric acid) and FORM+BUTR (basal diet with 0.1% formic and 0.03% butyric acid). At day 35 the birds were slaughtered and eviscerated, and from which the breast meat was obtained. Carcass and cut yields of broilers were not different (P>0.05) among CONT, FORM and BUTR birds. The combination of formic and butyric acids increased (P<0.05) the relative weight of back of broilers. Meat pH value was higher (P<0.05) in organic acids treated than in non-treated birds. Organic acids lowered (P<0.05) drip loss of broiler meat. Butyric acid increased (P<0.05) moisture and decreased (P<0.05) the content of meat protein. Formic acid increased (P<0.05), whereas butyric acid and combination of formic and butyric acids decreased (P<0.05) lightness values of breast meat. Yellowness values of meat increased (P<0.05) with organic acid administration. Intense bands of 25 kDa (phosphoglycerate mutase) was observed in most of meat samples from the treated birds, but not in meat from the control birds. In conclusion, dietary supplementation of organic acids was capable of improving the meat quality of broiler meats. The treatments may prevent the pale, soft and exudative (PSE)-like condition in broiler meats. 
EFFECT OF POST-HATCH FEED DEPRIVATION ON FATTY ACID COMPOSITION OF BROILER MEAT Sugiharto, S.; Isroli, I.; Yudiarti, T.; Widiastuti, E.; Kusumanti, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 4 (2015): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.4.234-237

Abstract

The objective of the present study was to investigate the effect of feed deprivation over the first 48 h post-hatch on the composition of fatty acids in broiler meat. Sixty 1-d-old Ross chicks were divided to two experimental groups, i.e., birds provided access to feed and water ad libitumimmediately post-hatch until d 35 and birds deprived from feed but not from water over the first 48 h post-hatch. Blood for cholesterol analysis was collected on d 34. The same birds were sacrificed on d 36, and immediately breast meat, abdominal fat, heart and liver were collected and weighed.Feed deprivation over the first 48 h post-hatch retarded (P<0.05) growth performance of chickens, but had no impact (P>0.05) on the abdominal fat, fatty acid profile of meat, total serum cholesterol level as well as the weight of heart and liver of broiler. In conclusion, early post-hatch feeding is a beneficial means to enhance the final body weight of broilers without affecting the meat quality.
EFFECT OF TURMERIC EXTRACT ON BLOOD PARAMETERS, FEED EFFICIENCY AND ABDOMINAL FAT CONTENT IN BROILERS Sugiharto, S.; Isroli, I.; Widiastuti, E.; Prabowo, N.S.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 1 (2011): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.1.21-26

Abstract

The experiment was carried out to investigate the effect of turmeric extract on blood parameters,feed efficiency and abdominal fat content in broilers. A total of 100 mixed-sexes Lohman chicks wereused in the experiment set up with completely randomized design of 5 different doses of turmeric extractgiven orally start from day-15 to day-35. The chicks given no turmeric extract were used as a control(T0), whereas T1, T2, T3 and T4 were the chicks given turmeric extract of 200, 400, 600 and 800mg/kg-live BW, respectively. Hemoglobin values of broilers given turmeric extract 800 mg/kg-live BWwas significantly higher (p<0.05) at week-3 and week-4, but not at week-5, compared to broilers giventurmeric extract with lower doses or control group. Turmeric extract did not affect significantly (p>0.05)the concentration of erythrocytes, the levels of hematocrit as well as FCR and abdominal fat content ofbroiler. Turmeric extract seemed to be able to improve the digestion and metabolism (of fat) resulted inenhanced production of hemoglobin in broilers. The hypolipidemic effect of turmeric that could hamperits potential to promote the growth of broilers per se might explain why turmeric could not improve thefeed efficiency of broiler.
The potential of Bacillus strains isolated from the rumen content of dairy cows as natural antibacterial and antioxidant agents for broilers Sugiharto, S.; Yudiarti, T.; Isroli, I.; Widiastuti, E.; Wahyuni, H.I.; Suprijatna, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.115-123

Abstract

The study was conducted to investigate the in vitro antibacterial activity and antioxidant-enhancing effect of Bacillus strains isolated from the rumen content of dairy cows. The antibacterial activity of Bacillus strains was assessed against Escherichia colibased on the agar-well diffusion method. To assess the antioxidant-enhancing effect, the Bacillusstrains was mixed (prepared as multi-strains) and used to ferment herbal medicine waste (HMW). The effect was compared with that of commercial probiotic Bacillus subtilis and non fermented HWM. Results showed that each Bacillus strain was able to inhibit the growth of E. coli. Fermentation with mixed Bacillus strains tended (P=0.07) to enhance the 2,2-diphenylpicrylhydrazyl (DPPH) radicals scavenging activity and total phenolics content of HMW. In contrast, fermentation with commercial Bacillus subtilis tended (P=0.07) to decrease DPPH radical scavenging activity and total phenol of HMW. Fermentation with both Bacillus preparations increased (P<0.05) the contents of crude protein and ash in HMW, while crude fat increased  (P<0.05) in HMW fermented with Bacillus subtilis. Fermentation either with Bacillus subtilis or mixed Bacillus strains increased (P<0.05) the fiber content of HMW. In conclusion, Bacillus strains isolated from the rumen content of dairy cows showed antibacterial activity and antioxidant-enhancing effect, therefore, they are potential as feed additive to substitute synthetic antibiotics and antioxidants for broilers raised under tropical conditions.
Growth, Physiological, and Intestinal Responses of Low-Weight Day-Old Broiler Chicks to Dietary Infertile Egg Powder Handayani, F. R.; Widiastuti, E.; Agusetyaningsih, I.; Yudiarti, T.; Sari, D. A. P.; Sugiharto
Tropical Animal Science Journal Vol. 48 No. 6 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.6.521

Abstract

The present study examined the consequences of supplementing the diets of low-weight day-old broiler chicks (DOC) with 4% infertile egg powder (IEP). The chicks were categorized into three groups namely; normal-weight DOC, low-weight DOC on a basal diet, and low-weight DOC on a diet that contained 4% IEP (T2). In hematological analysis, there was increase in hemoglobin levels, and mean corpuscular hemoglobin concentration (MCHC) compared to T2, but the MCV was less than T0 (p<0.05). There was improvement in the lipid metabolism, whereby triglyceride and HDL levels reduced and increased, respectively (p<0.05). T2 showed an improved antioxidant status; it had an increased activity of superoxide dismutase (SOD) and a decreased level of malondialdehyde (MDA) (p<0.05). Ileal lactic acid bacteria (LAB) and coliform counts were also substantially reduced in T2 vs. T0 (p<0.05), whereas both caecal coliform counts and LNE counts and the LAB-to-coliform ratio were significantly worse in T1 and T2 than T0 (p<0.05). Histological findings expressed the aspect of villus height-to-crypt depth (VH/CD) ratio that was greater in duodenum and ileum of T2 than T1, and deeper crypt in the jejunum in T0 than T1 (p<0.05). There was significant reduction in lesion score in duodenum and the jejunum as seen in T2 group (p<0.05). T2 also had much higher weight gain (p<0.05). per day, feed consumption and body weight at the end when compared to T1. To summarize the findings, the supplement of 4% IEP via the diet improved the physiological functioning and intestinal health status, causing the rise in body weights in low-weight broiler chicks.
Dietary Supplementation of Spirulina platensis as a Substitute for Antibiotics in Arab Chicken (Gallus turcicus) Hasna, N. A.; Widiastuti, E.; Agusetyaningsih, I.; Wulandari, E. C.; Murwani, R.; Yudiarti, T.; Sartono, T. A.; Sugiharto, S.; Wahyuni, H. I.
Tropical Animal Science Journal Vol. 47 No. 2 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.2.180

Abstract

This study was conducted to determine the effect of adding Spirulina platensis to replace zinc bacitracin on performance, egg quality, blood profile, total gut bacteria, and liver histopathology of local indigenous Arab chicken (Gallus turcicus). One hundred and eight 28-week-old laying hens were distributed randomly to three treatments: T0 (control diet), T1 (T0 + 1% S. platensis), and T2 (T0 + 0.04% zinc bacitracin). The treatment was applied for 49 days. S. platensis and zinc bacitracin decreased feed intake (p<0.05), but the egg mass had no significant effect; this provides a good improvement in feed conversion ratio (p<0.05). S. platensis helped maintain persistent egg production (p<0.05). S. platensis and zinc bacitracin provided the best results on haugh unit (p<0.05). S. platensis increased the yolk score (p<0.05). Zinc bacitracin decreased erythrocytes (p<0.05) but was not significantly different from S. platensis. S. platensis and zinc bacitracin increased mean corpuscular volume (MCV) (p<0.05). Blood chemical profile and total gut bacteria were not affected by the treatments. S. platensis was the best at maintaining liver’s health (p<0.05). This research concludes that S. platensis can efficiently optimize feed consumption, enhance performance, maintain egg quality, and protect the liver damage of Arab chicken. Therefore, S. platensis could be considered to replace the use of antibiotics.
Improvement of Performance, Blood Profiles, Gut Health, and Immune Response of Broilers Supplemented with Chitosan, Bacillus subtilis, or Their Combination Agusetyaningsih, I.; Kismiati, S.; Mangisah, I.; Widiastuti, E.; Wahyuni, H. I.; Yudiarti, T.; Sartono, T. A.; Sugiharto, S.
Tropical Animal Science Journal Vol. 47 No. 3 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.3.343

Abstract

The study aimed to investigate the effect of supplementing chitosan, Bacillus subtilis or blends of both on broiler growth performance, immune response, biochemical variables, and intestinal ecology of broiler chickens. Two hundred and eighty day-old chicks were distributed into 4 experimental groups, including T0 (control, basal feed), T1 (basal feed + chitosan 0.1% feed), T2 (basal feed + B. subtilis 0.01% feed), and T3 (basal feed + chitosan 0.1% feed + B. subtilis 0.01% feed). Variables measurements and sample collection were conducted on day 35. The T3 did not differ from T0 and T1, but they consumed less (p≤0.05) feed than T2 chickens. Feed conversion ratio (FCR) was lower (p≤0.05) in T1 and T3 compared to T0 and T2 groups. Leukocyte counts in T2 and T3 were higher (p≤0.05) than those in T0. The T1, T2, and T3 had higher (p≤0.05) lymphocyte counts than T0. The T3 had lower (p≤0.05) low-density lipoprotein (LDL) levels than the T0 group. The T2 had higher (p≤0.05) levels of total protein and globulin than T3 and T0. Compared to T0 and T3, serum albumin levels were greater (p≤0.05) in T1 and T2. The T3 had higher (p≤0.05) duodenal villi height than that in the other groups. The T2 and T3 chicks had higher (p≤0.05) Newcastle disease titer than T0 and T1. Compared to T2 and T3, T0 and T1 showed a worse (p≤0.05) microscopic structure of jejunum. The T2 and T3 showed lower (p≤0.05) lesion score in bursa of fabricius than that in the other groups. In conclusion, the blends of chitosan and B. subtilis resulted in improved FCR, higher leukocyte and lymphocyte counts, lower LDL, higher duodenal villi height, higher Newcastle disease titer, better microscopic structure of jejunum, and lower lesion score in bursa of fabricius of broiler chickens.