Article Per Year (5 Year)
p-Index From 2021 - 2026
0.408
P-Index
This Author published in this journals
All Journal Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)
Ratih Dewanti
Animal Science Department, Faculty Of Agriculture, Sebelas Maret University
Veterinary Other

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Keragaman genetik puyuh Jepang (Coturnix japonica) berdasarkan analisis sekuen DNA mitokondria gen Cytochrome-b Novisa Adimaka; Muhammad Rifki; Ratih Dewanti; Muhammad Cahyadi
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 2 (2019): Agustus
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.02.05

Abstract

Coturnix japonica is a Japanese quail which is commonly raised in Indonesia. The aim of the study was to determine the genetic diversity of Japanese quail (Coturnix japonica) based on complete sequence of mitochondrial DNA Cytochrome b gene analysis. This study used 12 samples of quail blood collected from two different village breeding centres (VBC) representing black and brown plumage lines. Total DNA genome was isolated from whole blood by following the Wizard® Genomic DNA Purification Kit protocol (Promega, USA). The DNA amplification process in this study was carried out using novel forward and reverse primers using Primer3 software. Additionally, PCR products were sequenced and analysed using MEGA 6.0 and DNAsp v.5 softwares. A total of 21 sequences consists of 12 sequences of quail sample and 9 reference sequences obtained from NCBI website were analyzed to construct phylogenetic tree and to evaluate genetic diversity of Japanese quails. The sequence analysis showed that Japanese quail population in this study had kinship with Francolinus pintadeanus and Coturnix chinensis with an 81% bootstrap value. The genetic distance among Japanese quails was ranged from 0.000 to 0.003. In addition, the mean of genetic distance for Japanese quail was 0.002 and the mean of genetic distance for 21 samples was 0.067. Tajima D'test values were -1.35536 for Japanese quail population and -1.38090 for whole population used in this study. The conclusion of this study was the Japanese quail had low genetic diversity based on complete sequence of Mitochondrial DNA Cytochrome b gene analysis that mean Japanese quail had low level of evolution
Reproduction and Production Performance of Swamp Buffalo (Bubalus bubalis) at the Small-Scale Holder Farmer in Sragen Regency, Central Java, Indonesia Nimas Alifia Nastiti; Joko Riyanto; Ratih Dewanti; Muhammad Cahyadi; Ari Kusuma Wati; Wari Pawestri; Yuli Yanti
Jurnal Sain Peternakan Indonesia Vol 18 No 3 (2023)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.18.3.140-147

Abstract

This research aimed to determine the performance of swamp buffalo reproduction and production at the small-scale holder farmer in Sragen Regency, Central Java, Indonesia, from June to September 2021. The method used in this research was a survey with a census (complete) sample determination. Data collection applies to interviews, observations and literature studies. Research parameters used in reproductive performance were calving interval (CI), service per conception (S/C), age of the first estrus, age of first birth and postpartum estrus. In contrast, production performance parameters were chest circumference, body length and estimated body weight. The result of research on reproductive performance showed that the average CI were 13.2±0.9 months, S/C 1.25±0.4 time, age of first estrus 2.7±0.6 years, age of first birth 3.7±0.5 years and postpartum estrus were 30–60 days. The result of research on the production of male buffalo body length was 205.3±1.7, and the body length of female buffalo was 127.2±4.3 cm, whereas the chest circumference of female was 205.3±1.7 cm and the chest circumference of the male was 189±2.8 cm. The estimated body weight of females and males were 516.7±7.7 kg and 445.2±11.9 kg. Based on the result of the research, the characteristics of swamp buffalo reproduction and production in Sragen Regency were classified as good.
Identification of the Membrane-Associated Transporter Protein (MATP) Gene Polymorphism as Candidate Mutation for Albinism in Japanese Quail Laksita Haniifah Pratiwi; Ratih Dewanti; Muhammad Cahyadi
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 2 (2023): August 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.02.09

Abstract

A pigmentation abnormality could be due to genetic mutation and trigger a disease related pigmentation deficiency such as albino which might be caused by mutation in membrane-associated transporter protein gene (MATP). The aim of this study was to identify polymorphism in the MATP exon 3 in Japanese quail. A total of nine Japanese quail consisting three brown plumage quail, three black plumage quail, and three albino quail were used in this study. Blood samples were used to extract the genomic Deoxyribonucleic acid (DNA) and used to amplify exon 3 regions of the MATP by polymerase chain reaction (PCR). The PCR products were then sequenced and analyzed. A total of 10 mutations were found, of which three mutations, g.4460G>A, g.4479G>A, and g.4514T>C, were within exon 3. These mutations were synonymous and non-synonymous. No specific mutation for albino was found in this study. In  summary those mutations did not specifically determine albinism in Japanese quail.
Co-Authors Ari Kusuma Wati Joko Riyanto Laksita Haniifah Pratiwi Muhammad Cahyadi Muhammad Rifki Nimas Alifia Nastiti Novisa Adimaka Wari Pawestri Yuli Yanti