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Journal : Borneo Journal of Medical Laboratory Technology

Perbandingan Pengukuran Kadar Vitamin C Menggunakan Spektrofotometri UV-Vis pada Panjang Gelombang UV dan Visible Khoirul Ngibad; Dheasy Herawati
Borneo Journal of Medical Laboratory Technology Vol 1 No 2 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.616 KB) | DOI: 10.33084/bjmlt.v1i2.715

Abstract

Vitamin C is a source of antioxidants which has a primary function to inhibit the occurrence of an oxidation reaction and inhibit a free radical. The source of vitamin C can be found in food and beverage. This study aims to compare the measurement of vitamin C levels using a UV-Vis spectrophotometer at UV and Visible wavelength. The method used in this study was the UV-Vis spectrophotometry method. The analysis of vitamin C levels at UV wavelength 266 nm and Visible wavelength 494 nm with a calibration curve range 0.3 to 0.8 mg/L. Validation of the methods studied included: linearity, detection limits, quantization limits, and precision. The results showed that the measurement of vitamin C levels at UV wavelengths resulted in the linearity of concentrations of 0.2 - 0.8 mg / L, the limit of detection of 0.05 mg/L, the limit of quantization of 0.17 mg/L and level of precision with RSD to 0.2015. On the other hand, the measurement of vitamin C levels in the Visible wavelength produced linearity from concentrations of 0.3 to 0.8 mg/L, the detection limit of 0.05 mg/L, quantization limit of 0.18 mg/L and the level of precision with RSD of 1.0489. Thus, the determination of vitamin C levels in samples using a UV-Vis spectrophotometer was preferred to be measured at UV wavelengths.
Kombinasi Rebusan Daun Salam dan Kemangi dalam Menurunkan Kadar Asam Urat Mus musculus Fahlia Nirmala; Kholifatuz Zumaroh; Novia Afriani Donatomo; Khoirul Ngibad
Borneo Journal of Medical Laboratory Technology Vol 2 No 1 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.798 KB) | DOI: 10.33084/bjmlt.v2i1.1088

Abstract

Indonesia is one of the world’s largest of biodiversity. Especially bay leaves (Syzygium polyanthum) and basil leaves (Ocimum basilicum). These plants can be used to reduce blood uric acid. This study aimed to determine the optimum dose that could reduce uric acid levels by providing a combination of decoction of basil leaves and bay leaves and to knew the phytochemical compounds that could reduce uric acid levels in basil leaves and bay leaves. The antihiperuricemia activity of boiled bay leaves and basil leaves was tested on male sex mice (Mus musculus) Balb / C strain, aged 8-10 weeks to reveal the effectiveness of bay leaves and basil leaves as blood uric acid-lowering. The dosage of decoction used is 1.5 glasses, 1 glass, and 0.5 glass from the shrinking of the cooking water which was originally 2 glasses. Tests were carried out on mice that experienced hyperuricemia induced by potassium oxonate 250 mg / kg BW or 5 mg / 20 g BB in mice intraperitoneally and mixed chicken liver, sprouts and melinjo. Measuring the level of urate is done using the POCT (Point Of Care Tasting) method. The results showed that combination of boiled bay leaves and basil leaves could reduce blood uric acid levels in mice around 0.5 - 1.0 mg / dl. The activity was effective in both doses, 0.5 glass and 1.5 glasses, but to get a significant decrease in uric acid levels this cooking water had to be consumed regularly and for a longer time.