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All Journal Jurnal Ilmu Pertanian Indonesia Hemera Zoa Media Kedokteran Hewan Jurnal Medik Veteriner Quantum Teknika DedikasiMU: Journal of Community Service
Eko Prasetyo Nugroho
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Religion Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Electrical & Electronics Engineering Engineering Environmental Science Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mathematics Mechanical Engineering Medicine & Pharmacology Social Sciences Veterinary

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Studi Eksperimental Hubungan Feeding di Mesin Bubut CMZ T-360 dengan Kekasaran Permukaan Material St 60 untuk Shaft Steady Rest Kurniawan, Agus; Samudra, Yudha; Nugroho, Eko Prasetyo; Eleganta, Florent Reno; Ganang, Gabriel; Krisnata, Hindi Chandra; Faturrahman, Ichsan
Quantum Teknika : Jurnal Teknik Mesin Terapan Vol 2, No 1 (2020): October
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/jqt.020115

Abstract

The research tested the effects between feeding and surface roughness on shaft of steady rest which will be worked in CMZ T-360’s lathe machine. Material of St.60 was turned into shaft of steady rest in 2 CMZT-360’s machines with several of feeding like 0.045, 0.056, 0.068, 0.079, 0.90 and 0,102 mm/rev. Then the work piece was tested the surface roughness of the work piece with using roughness tester and being seen the shape of the surface with using photo macro. The result of the roughness’s test showed that CMZ-T360’s lathe machine could reach Ra 1.55 µm until 4.79 µm with several of feeding. The work piece would be assembled in steady rest and being used to support 58 kg weigth in 2 hours to know the effect of surface roughness. After testing on steady rest, would be done photo macro on the specimen to make easy on visual analysis. From the data which is obtained can conclude that feeding variation which used on this research not so affecting the quality of surface roughness of the workpiece, the value of surface roughness that resulted from this research is mostly N8 (3,25 µm- 5,29 µm) and only a few that valued N7 (1,55 µm-2,53 µm) . The second conclusion is the surface roughness which quickly wornt-out after assembled at steady rest is the rough surface (4,79 µm become 3,84 µm) compared with the smooth surface (2,23 µm become 2,03 µm).
Preservasi Imago Jantan Ulat Sutera Liar Attacus Atlas (Lepidoptera: Saturniidae) Eko Prasetyo Nugroho; Damiana Rita Ekastuti; Raden Iis Arifiantini
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.27 KB)

Abstract

Wild silkworm (Attacus atlas) has high economic value. There is a problem in rearing wild silkworm Attacus atlas, the emergence of males and females imagoes are not the same. The aim of this research was to study the survival lifetime and the sperm of the male imago. This research used randomized design experiment, with two treatments of storage temperature (room temperature 27 °C and cool storage temperature 5 °C) and eight repetitions. Survival of male imago, sperm survival, and imago body weight had been measured. The results showed that cool temperature (5 °C) could to extend significantly (P<0,01) the survival lifetime of male imago and the sperm of male imago A. atlas up to 11.5 ± 0.7 days. There is a positive correlation between body weight and lifetime of male imago of wild silkworm A. atlas, so the lifetime of male imago is predicable from their body weight.
PAT-5 Update: Q Fever in Indonesia Agus Setiyono; Elok Puspita Rini; Handayu Untari; Eko Prasetyo Nugroho
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.027 KB)

Abstract

Q Fever is zoonotic disease caused by Coxiella burnetii, an intracellular obligate and negative Gram bacterium with pleomorphic shape (Kaplan and Bertagna 1955). Centers for Disease Control and Prevention (CDC) classify Coxiella burnetii as potential bioterrorism agent within B rank for its ability and characteristics (CDC 2013).The main reservoir animals for Q fever are ruminants (Maurin and Raoult 1999). Q fever infection whether in animal or human generally occur through inhalation, vector bites, or through oral ingestion—the last two routes are considered as secondary routes (Angelakis and Raoult 2010). Clinical symptoms of Q fever in both animal and human generally asymptomatic. Q fever can cause abortion in the third trimester of pregnancy and pneumonia in ruminants. Whether in human, acute Q fever can cause flu like syndrome and can develop into hepatitis, endocarditis, and for some severe chronic case, it caused death (Fournier et al. 1998). Office international des epizooties (OIE) classify Q fever into a re-emerging disease group (OIE 2010). Based on OIE data in 2012, the distribution of Q fever in animals occur in almost all country in the world including ASEAN (OIE 2012).Q fever was first discovered in Australia in 1935 (Kaplan and Bertagna 1955). Q fever disease transmission in animal occurs almost in every country in the world. Based on OIE data, Indonesia was classified as no information region (OIE 2012). World Health Organization (WHO) reported that Q fever was first found in cow serologically in Indonesia in 1953 (Kaplan and Bertagna 1955). Q fever in ruminants was again reported by Indonesian researcher between 2006 to 2015 in Bali, West Java, Jakarta, and Medan (Mahatmi et al. 2007; Setiyono et al. 2008; Nasution et al. 2015).Positive Q fever result was obtained especially from ex imported cows, thus it was suspected that cows from abroad might play main role of transmission of Q fever in Indonesia. However, there was also report of positive result in local ruminants, such as Bali cattle, goat, and sheep (Mahatmi et al. 2007). Researches in various country also stated of potential local ruminant as reservoir animal,  among them are Tibet sheep in China, Alpine and Saanen goat in Italia, camel (Camelus dromedaries) in Saudi Arabia, or Swedish dairy cattle in Swedia (Mohammed et al. 2014). Based on the historical study of Q fever in Indonesia, this research was performed by taken sample from both ex-import cattle and local ruminants in several region in East Java (Malang, Surabaya, Madura), Central Java (Boyolali), and West Java (Bogor, Bandung, Depok) which are known as regions with high population of ruminants in Indonesia.  So far, surveillance data of Q fever has not been recorded from these regions.
Immunohistochemical Detection of Coxiella burnetii in Cattle Spleen Organs from Ampel Slaughterhouse, Boyolali Regency Nugroho, Eko Prasetyo; Setiyono, Agus; Hadi, Upik Kesumawati; Winarsih, Wiwin; Astuti, Dwi
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.48-55

Abstract

Q-fever is a zoonotic bacterial disease that caused by Coxiella burnetii. These microorganism are gram negative and obligate intracellular bacteria. This study was conducted to detect C. burnetii in cattle organs which collected from Ampel slaughterhouse Boyolali Regency. In this study, spleen, heart, lung, liver and kidney were collected from 100 cattle. The samples were tested by immunohistochemical (IHC) method using polyclonal anti- C. burnetii antibodies. Immunohistochemical examination found the presence of C. burnetii in the cytoplasm of macrophage cells with specific brown color only in the spleen as many as 4 out of 100 cattle showing immunoreactive (4%). The four positive individual samples were from Simental cattle. These results indicate that Q-fever was found in local cattle in Boyolali Regency.
PELATIHAN PENGENDALIAN LALAT PENGGANGGU DENGAN MEMANFAATKAN BAHAN ALAMI DI SD DARUL ULUM KEBONSARI SURABAYA Kurniabudhi, Marek Yohana; Nugroho, Eko Prasetyo; Wahyuningtyas, Puput Ade
DedikasiMU : Journal of Community Service Vol 6 No 4 (2024): Desember
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/dedikasimu.v6i4.8446

Abstract

Lalat rumah (Musca domestica) merupakan hama sinantropik yang paling umum ditemukan di seluruh dunia dan berpotensi menjadi vektor mekanik bagi berbagai agen penyakit seperti bakteri, protozoa, dan telur cacing, terutama Soil Transmitted Helminth (STH). Keberadaan lalat pada bahan pangan dapat menyebabkan kontaminasi yang mengakibatkan penurunan mutu dan membahayakan kesehatan manusia yang mengonsumsinya. Salah satu fasilitas umum yang rentan terhadap kontaminasi ini adalah Sekolah Dasar, dimana peserta didiknya merupakan aset bangsa yang harus dilindungi. Upaya pengendalian lalat rumah di area perkotaan seringkali hanya difokuskan pada pemberantasan lalat dewasa, sehingga upaya deteksi dan pengendalian populasi fase larva masih terbatas. Edukasi terkait pentingnya kebersihan lingkungan serta penggunaan metode alami untuk pengendalian lalat menjadi sangat penting dalam konteks ini. Beberapa bahan alami seperti lilin aromaterapi, cuka apel, kantong bening berisi air, kayu manis, dan serai memiliki potensi untuk mengurangi populasi lalat di lingkungan sekolah. Program pengabdian masyarakat ini dilaksanakan di SD Darul Ulum Kebonsari Surabaya, dengan tujuan memberikan edukasi, meningkatkan kesadaran akan kebersihan lingkungan, serta mengevaluasi efektivitas bahan pengendali lalat secara berkala.
Molecular Detection of Bacterial Pathogens in Flies as Potential Vectors from Surabaya Traditional Markets Wahyuningtyas, Puput Ade; Kurniabudhi, Marek Yohana; Nugroho, Eko Prasetyo
Media Kedokteran Hewan Vol. 36 No. 3 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i3.2025.234-240

Abstract

Flies are insects that act as mechanical vectors in the spread of disease, especially in environments with poor sanitation, such as traditional markets. Surabaya has several traditional markets with relatively poor hygiene, which poses a risk of becoming breeding grounds for flies carrying pathogens. This study aims to identify fly species and detect the presence of pathogenic bacteria, especially Escherichia coli and Salmonella spp., using the Polymerase Chain Reaction (PCR) method. This study employed purposive sampling and collected samples at five traditional market locations throughout the city of Surabaya. Morphological identification revealed that Musca domestica (89.78%) was the dominant species, followed by Chrysomya megacephala (9.43%), Chrysomya saffranea (0.3%), Lucilia sp. (0.41%), and Sarcophaga sp. (0.08%). The result of bacterial culture on selective media and PCR confirmation showed the presence of Escherichia coli (100%) in fly samples, while in SSA media, which was continued with biochemical tests, the results did not detect Salmonella (0%) bacteria. These findings confirm that market flies, particularly Musca domestica, can serve as vectors of pathogenic bacteria and have the potential to transmit diseases to humans. Therefore, monitoring and controlling fly populations in traditional markets is necessary as a preventive measure to maintain public health.
Co-Authors Agus Kurniawan Agus Setiyono Damiana Rita Ekastuti Dwi Astuti Eleganta, Florent Reno Elok Puspita Rini Faturrahman, Ichsan Ganang, Gabriel Handayu Untari Krisnata, Hindi Chandra Kurniabudhi, Marek Yohana Puput Ade Wahyuningtyas R. Iis Arifiantini Samodra, Yudha Upik Kesumawati Hadi Wiwin Winarsih