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All Journal Buletin Veteriner Udayana Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Vitek : Bidang Kedokteran Hewan DedikasiMU: Journal of Community Service
Puput Ade Wahyuningtyas
Universitas Wijaya Kusuma Surabaya
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary

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Identifikasi Molekuler Blastocystis sp. pada Monyet Ekor panjang di Taman Nasional Alas Purwo Edward Yonas Kristijanto; Wiwik Misaco Yuniarti; Mufasirin Mufasirin; Poedji Hastutiek; Endang Suprihati; Boedi Setiawan; Puput Ade Wahyuningtyas; Doohan Mahendra
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p05

Abstract

Zoonotic diseases can be transmitted through close interactions between long-tailed monkeys. Blastocystis sp. is one of the parasites that can attack mammals and is most commonly found in the intestinal tract. This study aims to analyze the presence of infection and the phylogenetic tree of Blastocystis sp. in long-tailed monkeys in Alas Purwo National Park, Banyuwangi, East Java. Identification of Blastocystis sp. in this study using morphological and molecular methods. A total of 100 stools were examined microscopically using the floating method, showing that 61 samples were positive, followed by a PCR test with a target of 600bp. PCR results obtained three positive samples followed by squencing. Sequences processed in BLAST isolate samples TNAP2 and TNAP9 having homology with Blastocystis sp. Subtype 3 was 98-99%, while the TNAP7 isolate had a lower homology level of 78-79% and the level of phylogenetic analysis of the TNAP2 and TNAP9 isolates was related to Blastocystis sp. from the Philippines (KY610153.1) and Egypt (OP942294.1) and the TNAP7 isolate is related to Blastocystis sp. from Thailand (MH197670.1, MH197668.1). Isolate Blastocystis sp. from Alas Purwo National Park has high homology analysis results to Blastocystis type hominis from Rep. Czech and Chinese by 80-99%, it is possible to have a connection with the zoonotic problem.
Measurements and Accuracy of IgM and IgG Anti Phenolic Glycolipid-1 Levels in Blood Serum for Early Detection Mycobacterium leprae by using Enzyme-Linked Immunosorbent Assay (ELISA): A Reality of a Laboratory Abdullah, Salsabilla Putri Kinanti; Dinar Adriaty; Iswahyudi; Puput Ade Wahyuningtyas; Laura Navika Yamani; Medhi Denisa Alinda; Ratna Wahyuni; Cita Rosita Sigit Prakoeswa
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 2 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i2.43481

Abstract

Indonesia was the third most recent case of leprosy globally in 2020 with 11,173 people, after India and Brazil. Most of the leprosy manifestations are asymptomatic. This is possibly as subclinical leprosy which individuals without leprosy symptoms but have leprosy specific antibodies high levels, so it has the potential to become a transmission and disability. Therefore, an ELISA test need for early detection in preventing leprosy transmission. This study aims to measure IgM and IgG antibody levels in leprosy patients and assess the accuracy of the measurement results. This research is a cross-sectional study. Five patients' blood samples have analyzed for IgM and IgG anti-PGL-1 antibody levels by ELISA. Accuracy interpretation of this measurement based on the %CV. Antibody levels were classified based on the cut-off <605 u/ml as IgM seronegative or <630 u/ml as IgG seronegative, 605–1000 u/ml as low seropositive IgM or 630-1000 u/ml as low seropositive IgG, and >1000 u/ml as high seropositive IgM and IgG. Among five patients examined, 40% had high seropositive leprosy with anti-PGL-1 IgM and IgG antibody titers>1000 u/ml, and 60% of patients had seronegative leprosy. Accuracy in this ELISA test shows high accuracy with %CV <10% in the conversion of OD to antibody titer levels.  IgM and IgG Anti PGL-1 antibody titers by ELISA as one of the parameters in identifying patients at higher risk of leprosy. A significant portion of patients with high seropositive leprosy with high accuracy.
PELATIHAN PENGENDALIAN LALAT PENGGANGGU DENGAN MEMANFAATKAN BAHAN ALAMI DI SD DARUL ULUM KEBONSARI SURABAYA Kurniabudhi, Marek Yohana; Nugroho, Eko Prasetyo; Wahyuningtyas, Puput Ade
DedikasiMU : Journal of Community Service Vol 6 No 4 (2024): Desember
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/dedikasimu.v6i4.8446

Abstract

Lalat rumah (Musca domestica) merupakan hama sinantropik yang paling umum ditemukan di seluruh dunia dan berpotensi menjadi vektor mekanik bagi berbagai agen penyakit seperti bakteri, protozoa, dan telur cacing, terutama Soil Transmitted Helminth (STH). Keberadaan lalat pada bahan pangan dapat menyebabkan kontaminasi yang mengakibatkan penurunan mutu dan membahayakan kesehatan manusia yang mengonsumsinya. Salah satu fasilitas umum yang rentan terhadap kontaminasi ini adalah Sekolah Dasar, dimana peserta didiknya merupakan aset bangsa yang harus dilindungi. Upaya pengendalian lalat rumah di area perkotaan seringkali hanya difokuskan pada pemberantasan lalat dewasa, sehingga upaya deteksi dan pengendalian populasi fase larva masih terbatas. Edukasi terkait pentingnya kebersihan lingkungan serta penggunaan metode alami untuk pengendalian lalat menjadi sangat penting dalam konteks ini. Beberapa bahan alami seperti lilin aromaterapi, cuka apel, kantong bening berisi air, kayu manis, dan serai memiliki potensi untuk mengurangi populasi lalat di lingkungan sekolah. Program pengabdian masyarakat ini dilaksanakan di SD Darul Ulum Kebonsari Surabaya, dengan tujuan memberikan edukasi, meningkatkan kesadaran akan kebersihan lingkungan, serta mengevaluasi efektivitas bahan pengendali lalat secara berkala.
Exploring Phylogenetic Variability of Blastocystis sp. Isolates in Macaca fascicularis in Indonesia Wahyuningtyas, Puput Ade; Kristijanto, Edward Yonas
VITEK : Bidang Kedokteran Hewan Vol 14 No 2 (2024): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v14i2.308

Abstract

found in both animals and humans. Long-tailed monkeys have the potential to be a reservoir for the spread of zoonotic diseases. This study aims to molecularly identify Blastocystis sp. The 18S SSU rRNA gene can be used to determine the diversity of subtypes, homology, and phylogenetic analysis of Blastocystis sp. 85 fecal samples of long-tailed monkeys from Alas Purwo National Park, East Java were identified and molecularly confirmed using PCR with the primers BhRDr (5'- GAG CTT TTT AAC TCC AAC AAA CG-3') and RD5 (5'-ATC TGG TTG ATC CTG CCA GT-3'). The PCR results showed three positive samples with amplified bands with a length of 600bp, then DNA sequencing was carried out to analyze the phylogenetics of Blastocystis sp. The sequencing results for sample codes MB7, MB22, and MB9 have the closest level of homology to subtype 1 found in human samples and long-tailed monkeys in several countries. The third isolate from this study was still identical, and considered a local strain species because the third sample was grouped in a separate branch from another country.
Diversity and potential of nuisance flies as vectors of gastrointestinal parasitic diseases in traditional markets in Surabaya City Kurniabudhi , Marek Yohana; Nugroho , Eko Prasetyo; Wahyuningtyas, Puput Ade
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 15 No. 2 (2025): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v15i2.545

Abstract

Nuisance flies are known mechanical vectors capable of transmitting bacterial agents, protozoa, and helminth eggs—particularly Soil-Transmitted Helminths (STH)—from traditional market environments to human food. This study aimed to identify the diversity and distribution of nuisance fly species and to investigate the presence of gastrointestinal parasites in these flies at traditional markets in Surabaya City. Fly samples were collected from five traditional markets and examined using native, sedimentation, and flotation techniques under a microscope. Descriptive statistics were employed to analyze species diversity, relative Abundance, frequency, dominance, and Shannon-Wiener diversity index (H'). The identified species included Chrysomya megacephala, Chrysomya saffranea, Lucilia sp., Musca domestica, and Sarcophaga sp., with M. domestica being the dominant species across all markets. Endoparasitological examination revealed the presence of protozoa and helminth eggs. Entamoeba sp. was consistently detected (100%) in both fly genera, while Isospora sp. oocysts were exclusively found in M. domestica from Wonokusumo Market. Ancylostoma sp. and Toxocara sp. eggs were identified in M. domestica from several markets and in Chrysomya sp. from Kaliwaron LKMK Market. The presence of these parasites is likely due to the flies’ contact with contaminated substrates such as sewage, market waste, animal carcasses, or vegetables. These findings underscore the importance of nuisance fly surveillance, as these insects not only cause discomfort but also act as vectors of zoonotic pathogens posing health risks to animals and humans.
Molecular Detection of Bacterial Pathogens in Flies as Potential Vectors from Surabaya Traditional Markets Wahyuningtyas, Puput Ade; Kurniabudhi, Marek Yohana; Nugroho, Eko Prasetyo
Media Kedokteran Hewan Vol. 36 No. 3 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i3.2025.234-240

Abstract

Flies are insects that act as mechanical vectors in the spread of disease, especially in environments with poor sanitation, such as traditional markets. Surabaya has several traditional markets with relatively poor hygiene, which poses a risk of becoming breeding grounds for flies carrying pathogens. This study aims to identify fly species and detect the presence of pathogenic bacteria, especially Escherichia coli and Salmonella spp., using the Polymerase Chain Reaction (PCR) method. This study employed purposive sampling and collected samples at five traditional market locations throughout the city of Surabaya. Morphological identification revealed that Musca domestica (89.78%) was the dominant species, followed by Chrysomya megacephala (9.43%), Chrysomya saffranea (0.3%), Lucilia sp. (0.41%), and Sarcophaga sp. (0.08%). The result of bacterial culture on selective media and PCR confirmation showed the presence of Escherichia coli (100%) in fly samples, while in SSA media, which was continued with biochemical tests, the results did not detect Salmonella (0%) bacteria. These findings confirm that market flies, particularly Musca domestica, can serve as vectors of pathogenic bacteria and have the potential to transmit diseases to humans. Therefore, monitoring and controlling fly populations in traditional markets is necessary as a preventive measure to maintain public health.
Co-Authors Abdullah, Salsabilla Putri Kinanti Boedi Setiawan Cita Rosita Sigit Prakoeswa DINAR ADRIATY Doohan Mahendra Edward Yonas Kristijanto Eko Prasetyo Nugroho Endang Suprihati Iswahyudi Kristijanto, Edward Yonas Kurniabudhi , Marek Yohana Kurniabudhi, Marek Yohana Laura Navika Yamani Medhi Denisa Alinda, Medhi Denisa Mufasirin Nugroho , Eko Prasetyo Poedji Hastutiek Ratna Wahyuni Wiwik Misaco Yuniarti