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All Journal Journal of Applied Data Sciences Biological Environment and Pollution Jurnal Kedokteran Hewan Biosaintifika: Journal of Biology & Biology Education Proceeding of International Conference on Biology Education, Natural Science, and Technology
Anni Nurliani
Program Studi Biologi FMIPA Universitas Lambung Mangkurat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Physics Veterinary

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Morphological  Characteristics and Nest Structure of Stingless Bee (Heterotrigona itama) from Different Meliponiculture Practices Kadarsah , Anang; Putra, Aminuddin Prahatama; Nurliani, Anni; Suhartono, Eko; Ibrahim, Sayed
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.3503

Abstract

Exploration of Heterotrigona itama biology characteristics besides being valuable for health and biodiversity also beneficial as well as an environmental bioindicator. This research aims to evaluate morphology characteristics and nest structure of stingless bee Heterotrigona itama from three different meliponiculture practices (MP1:meliponiculture in the back yard; MP2:meliponiculture in the rubber garden and MP3: meliponiculture in the side yard) in Banjar Regency, Indonesia. The parameters studied are morphology, nest design, colony structure, and environmental suitability. One-way ANOVA at a 95% confidence level was used to analyze differences between parameters. The results show Heterotrigona itama body size average from rubber gardens is bigger (7.11±0.60 mm) than the side yard (6.79±0.34 mm) and back yard (6.64±0.46 mm). The degree of difference is visible in the fore leg (α=0.00<0.05) and hind leg (α=0.004<0.05). The nest structure in MP3 was significantly different than MP1 and MP2, especially in terms of funnel length (p=0.007<0.05) and nest height from the ground  (p=0.000<0.05).  Environmental conditions MP3  (temperature 31.69±1.93 0C, humidity 60.70±11.2 % and water source) is more supportive for meliponiculture than MP2 (disturbed by household waste) and MP3 polluted by chicken farms. Meliponicultures practices based on various locations and environmental characteristics influence several parameters of Heterotrigona itama morphology and nest structure.
Realtime and Spatial Data Analysis-based Monitoring System for Proboscis Monkey Habitat Health to Enhance Conservation Area Management Effectiveness Nurliani, Anni; Krisdianto, Krisdianto; Rezeki, Amalia; Munsyi, Munsyi
Journal of Applied Data Sciences Vol 5, No 4: DECEMBER 2024
Publisher : Bright Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47738/jads.v5i4.334

Abstract

The conservation of proboscis monkeys (Nasalis larvatus), an endemic primate species of Borneo, faces significant threats due to habitat degradation and declining populations. This study aims to develop a real-time and spatial data analysis-based monitoring system to improve the management of conservation areas for the species’ natural habitats. Conducted in the wetland ecosystems of Curiak Island, South Kalimantan, the research integrates remote sensing, Geographic Information Systems (GIS), and Internet of Things (IoT) technologies to monitor key environmental parameters such as vegetation health, land surface temperature (LST), and others. Indices like the Normalized Difference Vegetation Index (NDVI), Vegetation Health Index (VHI), Vegetation Condition Index (VCI), Temperature Condition Index (TCI), and Environmental Critical Index (ECI) are utilized to assess habitat conditions. Initial results showed poor vegetation health, with an NDVI of 0.6085, high LST of 20.41°C, and considerable environmental stress, reflected by an ECI of 74. Restoration efforts, however, improved conditions, with the NDVI rising to 0.7288, LST decreasing to 20.75°C, and the ECI lowering to 53 in the restoration area, signaling recovery. Though the ECI still suggests moderate environmental stress, the trend is positive. IoT sensors provided continuous real-time data, including CO levels at 0.2 PPM, CO2 at 34,045 PPM, O2 at 20.4% Vol, temperatures ranging from 33.155°C to 33.185°C, humidity between 67.45% and 67.65%, and pH at 6.8. Data on dissolved oxygen, total dissolved solids (TDS), and turbidity were also collected, providing dynamic insights into environmental conditions. The integration of community-based approaches ensures sustainable conservation efforts through local participation. This comprehensive monitoring system supports both proboscis monkey conservation and broader ecological objectives like biodiversity preservation, climate change mitigation, and ecosystem service provision, emphasizing adaptive management in conservation strategies.
Microplastic contamination in Heterotrigona itama bee products (Honey, Pollen, Propolis) from Meratus Geopark, South Kalimantan Kadarsah, Anang; Putra, Aminuddin Prahatama; Nurliani, Anni; Suhartono, Eko
Biological Environment and Pollution Vol. 5 No. 1 (2025)
Publisher : Association for Scientific Computing, Electronics, and Engineering (ASCEE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31763/bioenvipo.v5i1.904

Abstract

Microplastic pollution has become a significant environmental concern, with recent studies detecting microplastics in various ecosystems and natural products, including those produced by bees. This study investigates the presence, morphology, and distribution of microplastics in products of the stingless bee Heterotrigona itama—specifically honey, pollen, and propolis—collected from six meliponiculture sites within the Meratus Geopark, South Kalimantan, Indonesia. Samples were analyzed using stereomicroscopy to identify microplastic types, quantify particles, and measure sizes. The findings reveal that fibers and fragments are the predominant microplastic forms, with urban locations exhibiting higher contamination levels (approximately 309 to 318 particles per 100 ml) compared to rural areas. Notably, one rural site recorded the highest contamination level (approximately 362 particles per 100 ml), suggesting that factors beyond urbanization, such as agricultural practices and atmospheric deposition, contribute to microplastic pollution. The study underscores the role of bees as natural bioindicators for environmental monitoring and highlights the potential risks of microplastic contamination to bee health, food safety, and ecosystem sustainability. These insights are aligned with Sustainable Development Goal 12, which advocates for responsible consumption and production.
STUDI HISTOKIMIA LEKTIN TERHADAP JENIS DAN DISTRIBUSI GLIKOKONJUGAT ABOMASUM KERBAU RAWA (Bubalus bubalis) KALIMANTAN SELATAN Nurliani, Anni; Budi Pitojo, Teguh; Kusindarta, Dwi Liliek
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2826

Abstract

Penelitian ini bertujuan mengkaji efisiensi pencernaan kerbau rawa dengan mengidentifikasi jenis dan distribusi glikokonjugat pada daerah abomasum kerbau rawa. Enam ekor kerbau rawa jantan 2,5 tahun dan berat badan 300-400 kg digunakan dalam penelitian ini. Sampel diperoleh dari rumah potong hewan (RPH) Kabupaten Banjar, Kalimantan Selatan. Setiap bagian abomasum meliputi kardiak, fundus, dan pilorus diambil untuk pengamatan mikroskopis dengan pewarnaan hematoksilin-eosin (HE) dan alcian blue-periodic acid schiff (AB-PAS). Residu gula glikokonjugat pada abomasum dideteksi dengan pewarnaan histokimia lektin dengan menggunakan wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin agglutinin (Con A), ulex europaeus agglutinin (UEA), dan soybean agglutinin (SBA). Hasil penelitian menunjukkan bahwa daerah kardiak mengandung glikokonjugat D manosa/D glukosa, D galaktosa, dan N asetilglukosamin. Daerah fundus mengandung D manosa/D glukosa, D galaktosa, L fukosa, N asetilglukosamin, dan N asetilgalaktosamin. Daerah pilorus mengandung glikokonjugat L fukosa dan N asetilglukosamin. Pola reaktivitas daerah kardiak, fundus, dan pilorus kerbau rawa terhadap pewarnaan histokimia lektin memiliki pola yang berbeda dengan ruminansia lain. Jenis glikokonjugat yang dimiliki oleh kerbau rawa tersebut diduga berkaitan dengan fungsi peningkatan kemampuan efisiensi pencernaan kerbau rawa. Setiap bagian abomasum kerbau rawa memiliki jenis glikokonjugat yang spesifik dengan pola distribusi khas sesuai dengan fungsinya.
Health Examination of The Digestion of Proboscis Monkey (Nasalis larvatus) through Bacteriological Tests on Feces Maisya, Camalia; Nurliani, Anni; Rezeki, Amalia; Rusmiati, R
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

This study aims to identify bacteria and the prevalence value of bacteria found in probosci's monkey feces as a picture of proboscis monkey digestive health. Seventeen fecal samples from each proboscis monkey, namely Mimin, Chikita, and Pedro, were taken at the Bekantan Rescue Center, Sahabat Bekantan Indonesia Foundation (SBI). Feces were identified by making macroscopic and microscopic observations. Visible observations include consistency, color, mucus, and blood in the physical feces. Microscopic observations were made by culturing feces on Mac Conkey, blood agar, EMBA, DHL agar, and NA media, followed by gram staining and biochemical tests consisting of VP (Voges-Proskaeur) test, methyl red test, indole test, citrate test, and lactose test. The results of identifying 17 feces samples from each probosci's monkey found the presence of E.coli bacteria from microscopic testing. The prevalence of E.coli bacteria in Mimin, Chikita, and Pedro is 41.17%; 35.29%; 35.29%, so it is concluded that the presence of E.coli is a typical bacterium that is not harmful to the digestion of proboscis monkeys, supported by observations on the physical macroscopic feces of good probosci's monkeys.
Identification of Intestine Parasite Worms Eggs in Feces Proboscis Monkey (Nasalis larvatus) Rahman, Fiqita Giulleta Irtifannisa; Nurliani, Anni; Rezeki, Amalia; Rusmiati, R
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

The proboscis monkey (Nasalis larvatus) is one of the endemic primates on the island of Borneo which is protected in Indonesia. Rehabilitation efforts are important to prevent extinction of the proboscis monkey. Parasitic diseases are diseases that often infect primates in rehabilitation centers. Examination feces is one way to diagnose the presence of parasitic worms in the animal's body. This study aims to identify and know Prevalence of types of parasitic worms found in the feces of proboscis monkeys at the Bekantan Rescue Center Sahabat Bekantan Foundation (SBI). Sampling of proboscis monkey feces was carried out for 20 days on three proboscis monkeys at SBI. Identification of endoparasites is carried out in the Parasitology Laboratory, Banjarbaru Veterinary Center, South Kalimantan, using the Whitlock sedimentation method. The results of this study indicate that there are types of worms Trichuris trichiura, Trichostrongylus sp. and Oesophagostomum sp. found in proboscis monkey feces samples. The highest prevalence was found in the type of worm Trichuris trichiura which was found in Mimin's proboscis monkey, which was 35%.
Co-Authors Amalia Rezeki Aminuddin Prahatama Putra Anang Kadarsah, Anang Dwi Liliek Kusindarta Eko Suhartono Ernawati Ernawati Heri Budi Santoso Ibrahim, Sayed Kadarsah , Anang Krisdianto Sugiyanto Maisya, Camalia Munsyi, Munsyi Rahman, Fiqita Giulleta Irtifannisa Rusmiati, R Teguh Budi Pitojo, Teguh