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AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL KULIT JERUK KINTAMANI (Citrus aurantium L.) DALAM MENURUNKAN KETENGIKAN MINYAK KELAPA P Oka Sudiana; I M Oka Adi Parwata; J J Sibarani
Jurnal Kimia (Journal of Chemistry) Vol. 12 No.1 Januari 2018
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.475 KB) | DOI: 10.24843/JCHEM.2018.v12.i01.p01

Abstract

Paper ini membahas penentuan aktivitas antioksidan dan pengaruh penambahan ekstrak etanol kulit buah jeruk kintamani (EEKBJK) dalam menurunkan ketengikan minyak kelapa yang sudah dipanaskan pada suhu 200oC, 250oC, 300oC selama 5 menit kemudian didiamkan selama 3 hari.Kerusakan pada minyak kelapa dilihat dari nilai bilangan peroksida, bilangan asam dan kadar asam lemak bebas. Hasil pengukuran aktivitas antioksidan menunjukkan bahwa IC50 dari EEKBJK sebesar 53,71 mg/L. Hal ini menunjukkan EEKBJK mampu sebagai antioksidan. Penurunan masing-masing bilangan peroksida minyak kelapa dengan penambahan EEKBJK 0,02% b/v adalah 50,19%, 31,74% dan 16,01%. Penurunan masing-masing bilangan asam minyak kelapa dengan penambahan EEKBJK 0,02% b/v adalah 29,55%, 28,21% dan 18,42%. Penurunan masing-masing kadar FFA minyak kelapa dengan penambahan EEKBJK 0,02% b/v adalah 29,55%, 28,21% dan 18,42%.
ISOLASI DAN UJI ANTIRADIKAL BEBAS MINYAK ATSIRI PADA DAUN SIRIH (Piper betle Linn) SECARA SPEKTROSKOPI ULTRA VIOLET-TAMPAK I M. Oka Adi Parwata; Wiwik Susanah Rita; Raditya Yoga
Jurnal Kimia (Journal of Chemistry) Vol. 3, No. 1 Januari 2009
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.04 KB)

Abstract

Isolation, identification and free radical activities testing of essential oil from Piper betle Linn were carriedout. Around 13,0 mL (12, 37 gram) of yellow essential oil was obtained from 10,0 kg fresh leaves of Piper betleLinn. The oil was then partitioned with MeOH : H2O (7:3), n-hexane and chloroform.Free radical activities testing showed n-hexana fraction was the most active which can reduced 66,27 % ofDPPH for 5 minutes and 89,13 % in 60 minutes. GC-MS analysis revealed that there were 27 peaks. Based on thedata analysis there were only 9 compound, i.e. 4-metil(1-metiletil)-3-sikloheksen-1-ol, 1-metoksi-4(1-propenil)benzene, 4-(2-propenil)fenol/kavicol, 4-alilfenilacetate, Eugenol, Karyofilen, 3-alil-6-metoksifenilasetat, 4-alil-1,2-diasetoksibenzena dan dekahidro-4a-metil-1-metilen-7(1-metiletenil) naftalena.Based on peak’s intensity the oil were dominated by 4 compound i.e. 4-allyl phenyl acetate, 2 methoxy-4-(2 prophenil) fenol/eugenol, 3-allyl-6-methoxy phenyl acetate, 4-(2-prophenyl)-phenol / cavikol.
PENENTUAN KANDUNGAN TOTAL FLAVONOID DAN TOTAL FENOL DARI AKAR KERSEN (Mutingia calabura) SERTA AKTIVITASNYA SEBAGAI ANTIOKSIDAN M. R. M. Senet; I G. M. A. P. Raharja; I K. T. Darma; K. T. Prastakarini; N. M. A. Dewi; I M. Oka Adi Parwata
Jurnal Kimia (Journal of Chemistry) Vol. 12 No.1 Januari 2018
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.661 KB) | DOI: 10.24843/JCHEM.2018.v12.i01.p03

Abstract

Penelitian ini bertujuan untuk mengetahui kadar fenol dan flavonoid total dari ekstrak akar kersen serta aktivitas antioksidannya. Hasil ekstraksi 1 kg akar kersen menghasilkan ekstrak etanol (30,56 g), kloroform (6,52 g), etilasetat (6,1 g), n-butanol (3,21 g) dan air (4,65 g). Penapisan fitokimia menunjukkan ekstrak yang positif flavonoid adalah ekstrak air, kloroform, etanol dan etil asetatsedangkankelimaekstrak tersebut positif mengandung polifenol. Ekstrak etanol, n-butanol, kloroform, etil asetat, dan air memiliki kandungan total fenol berturut-turut yaitu 12,62%, 3,81%, 6,12%, 8,75%, dan 11,93%. Kandungan flavonoid pada ekstrak etanol, kloroform, etil asetat, dan air berturut-turut yaitu 0,22%, 0,05%, 0,12%, dan 0,03%. Aktivitas antioksidan pada ekstrak etanol diukur dengan metode DPPH dan dinyatakan dengan nilai IC50 sebesar 36,44 ppm
AKTIVITAS LARVASIDA MINYAK ATSIRI PADA DAUN SIRIH (Piper betle Linn) TERHADAP LARVA NYAMUK Aedes aegypti I Made Oka Adi Parwata; Sri Rahayu Santi; I Made Sulaksana; Ida Ayu Alit Widiarthini
Jurnal Kimia (Journal of Chemistry) Vol. 5, No. 1 Januari 2011
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation and larvacide activity of essential oil from Piper betle Linn were studied. Approximately 13,5 mL of essential oil was obtained from 10,0 kg fresh leaves of Piper betle Linn. The oil is pale yellow with density of 0,7148 g/mL. Phytochemical test indicates that the content of the oil are terpenoid and phenol compounds with toxicity Aedes aegypty larvae 0f LC50= 309,03 ppm. The essential oil was toxic towards Aedes aegypty larvae because it contains phenol compounds, i.e. Eugenol, cavichol.GC-MS analysis reveals that there are 9 compound, i.e. 4-methyl(1-methylnethyl)-3-cyclohexen-1-ol, 1-methoxy-4(1-propenyl) benzene, 4-(2-propenyl)phenol/cavicol, 4-alylphenylacetate, Eugenol, Karyofilen, 3-alyl-6-methoxyphenylacetate, 4-alyl-1,2-diacetoxybenzene and decahydro-4a-methyl-1-methylene-7(1-methylethenyl) naphthalene.Based on the peaks intensity the oil was dominated by 4 compounds i.e. 4-allyl phenyl acetate, 2 methoxy-4-(2 prophenyl) phenol/eugenol, 3-allyl-6-methoxy phenyl acetate, 4-(2-prophenyl)-phenol / cavicol. These four compounds probably act as the active compounds to kill Aedes aegypty larvae, as they are acetate and phenol compounds.
ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DARI RIMPANG LENGKUAS (Alpinia galanga L.) I M. Oka Adi Parwata; P. Fanny Sastra Dewi
Jurnal Kimia (Journal of Chemistry) Vol. 2, No. 2 Juli 2008
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation and antibacterial activity test of the languas rhizomes (Alpinia galanga L.) essential oil was carriedout The extract was yellow in colour with a volume of 3,5 mL.The activity test on E. coli bacteria at concentrations 100 ppm and 1000 ppm showed a retardation area of 7mm and 9 mm in diameters. At 1000 ppm it retarded S. aureus bacteria to a diameter of 7 mm. Gas Chromatography– Mass Spectrometer data show that the essential oil of the languas rhizomes contains mainly 8 components : Dlimonen;Eukaliptol; 3-sikloheksen-1-ol, 4-metil-1-(1-metietil); Fenol, 4-(2-profenil)-asetat; 2,6-oktadien-1-ol, 3,7-dimetil-asetat; 1,6,10-dodekatrien, 7,11-dimetil-3-metilen; Pentadesen; sikloheksen, 1-metil-4-(5-metil-1-metilen-4-heksenil.
PENGARUH KONSENTRASI EKSTRAK KASAR KULIT BATANG KUSAMBI (Schleichera oleosa (Lour) Oken) TERHADAP PERTUMBUHAN IN VITRO BAKTERI E. coli Ni Made Susilawati; Yan Ramona; I Made Oka Adi Parwata
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p06

Abstract

The main objective of research was to investigate the effectiveness of Schleichera oleosa (Lour) Oken crude extract to inhibit the in vitro growth of E. coli. The crude extract of the plant and the E. coli used in this study were obtained by applying the method of maceration in methanol solution and the method of dilution and spread plate on nutrient agar (NA) medium, respectively. The in vitro bioassay of the extract on E. coli was conducted by applying the method of diffusion on NA medium. The results showed that the crude extract of Schleichera oleosa (Lour) Oken was found to be effective to inhibit the growth of E. coli in the in vitro tests. The diameter of inhibition zone was proportional with the extract concentration exposed to the E. coli.
Formulasi Inokulan Jamur Pembentuk Gubal Gaharu pada Tanaman Ketimunan (Gyrinops versteegii) I MADE MEGA; DEWA KETUT SUANDA; DESAK NYOMAN KASNIARI; WAYAN SUENA; MADE ADI OKA PARWATA
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

This study aims to find the most effective formulation of the gaharu agar wood forming fungi inoculantswhich infect Gyrinops versteegii plants, thus producing high quality agar wood. The method used inthis research is the randomized block design (RBD) with three replication. The treatments were twofungi as inoculant applied saperately each and a combination of Rhisopus sp. and Fusarium sp. in theform of solid and liquid. The treatments tested were: (A) Fusarium solani fungi inoculant in solid form,(B) Rhisopus sp.fungi inoculant in solid form, (C) Mixture of Rhisopus sp. and Fusarium solani fungiinoculants in solid form, (D) Fusarium solani fungi inoculant in liquid form, (E) Rhisopus sp.fungiinoculants in liquid form, (F) Mixture of Rhisopus sp. and Fusarium solani fungi inoculants in liquidform, and (G) A combination of solid and liquid of Rhisopus sp. and Fusarium solani fungi inoculants.The parameters measured were the agar wood formed: agar wood fragrance level, color of agar woodand resin content of the agar wood. Furthermore, the data is analyzed statistically by analysis ofvariance. The results showed that, treatments of inoculants formulations significantly affects the aromaand resin content of agar wood. Formula of G (a combination of solid and liquid Rhisopus sp. andFusarium solani fungi inoculants) and F (a combination of liquid Rhisopus sp. and Fusarium solanifungi inoculants) produced agar wood of the highest quality compared to other formulas. Formula Gand F produced each agar wood with black and blackish-brown colour, with strong aroma (fragrance),and a resin content of 7.69% and 6.98%.
Determination Ethanol In Arak With Gas Chromatography Nyoman Sudarma; I Made Oka Adi Parwata
Bali Medika Jurnal Vol 4 No 2 (2017): Bali Medika Jurnal Vol 4 No 2 Desember 2017
Publisher : Stikes Wira Medika Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36376/bmj.v4i2.10

Abstract

Ethanol is a type of alcohol that can be consumed. One of drinks containing ethanol is Arak. Due to ethanol drink containing in excessive levels, has many causes and event such as accidents caused by drunken brawl. The purpose of this research was to determine the levels of ethanol in Arak is sold in the Merita village of Karangasem regency. Determination levels of ethanol done by gas chromatography. Results of the determination of levels ethanol of 5 samples taken at random to meet the standard required by Menkes RI number  86/Menkes/Per/IV/77, alcohol group B at the rate of 5-20% is sampel ‘a’ that is at the rate of 18.47%, sample ‘c’ with the  rate of 16.11% and sampel ‘d’ with the rate 11.68%. Alcohol group C with 20-55% rate that is in the sample ‘b’ with a sample rate of 25.28% and sampel ‘e’ with the rate 25.94%.
ANALISIS KADAR KLORIDA PADA AIR SUMUR GALI DI BANJAR TELAGA, DESA KUTAMPI KALER, KECAMATAN NUSA PENIDA, KABUPATEN KLUNGKUNG DENGAN TITRASI ARGENTOMETRI I Wayan Pira Adi Pratama; I Made Oka Adi Parwata; Putu Gede Subhaktiyasa
Bali Medika Jurnal Vol 4 No 1 (2017): Jurnal Chemistry Laboratory Vol 4 No 1 July 2017
Publisher : Stikes Wira Medika Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36376/bmj.v4i1.51

Abstract

Introduction : Water is the main of live for the living things especially human being. The function of water for living can’n be change by another soul. In coatal area is the area that often get difficulties to get clean water access, because often happen of instruction of sea water that cause high of clorida content. In plain water clorida ion at water work can cause corosi vitas on instrument that made from metal and salty taste on the water. The aim of the research to determine degree of clorida ion and analisis of quality of well water that dig by the inhabitant at banjar telaga kutampi village kaler nusa penida district, Klungkung regency. Method : The determining of clorida ion degree do by titrasi argentometri method mohr. Clorida degree from five sample dig well water in mg/L unit in succession: 310,8255, 1.196,5347, 1.716,0163, 560,1336, and 1.185,0225. Result : The things that can polluted well water is that the nearest distance of well with can raised clorida degree in well water. Because well water near with the beach will easy get sea water instruction or leak of sea water to well water. Based on Permenkes Republic of Indonesia number 492/Menkes/Per/IV/2010. The limit of clorida ion maximum 250 mg/L where the sample of degree that can be say the quality of five well water sample is not good from its clorida degree
PENENTUAN KADAR SENYAWA ORGANIK MATA AIR DI DESA PESEDAHAN, KARANGASEM SECARA PERMANGANOMETRI I Made Arya Wirya Atmaja; I Made Oka Adi Parwata; Putu Gede Subhaktiyasa
Bali Medika Jurnal Vol 4 No 1 (2017): Jurnal Chemistry Laboratory Vol 4 No 1 July 2017
Publisher : Stikes Wira Medika Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36376/bmj.v4i1.52

Abstract

Introduction: Water is the main component / important for living things, so its quality and quantity needs to be maintained. Similarly, the springs in the Pesedahan Village, Manggis District, Karangasem Regency that gives an important influence for the community’s activities there.Water quality decline to a certain level caused by one of the organic compounds contamination. The decrease of water quality to a certain level is caused by one of the organic compounds. Method: This research is a quantitative research. The purpose of this research is to determine and analyze the level of organic compound spring in Pesedahan Village, Manggis Subdistrict, Karangasem Regency. The research was conducted on 5 springs in the location, each taken 2 L as sample. Result: The result of the research by permanganometri result showed that the data of organic compound samples 1, 2, 3, 4, and 5 sequentially in mg / L were 8,2581, 8,8481, 11,0178, 9,8381 and 6,6781, respectively With Permenkes R1 No.492 / Menkes / Per / IV / 2010 found that the organic compound of sample 3 exceeded the maximum limit, while the other four samples were below maximum. Discussion: For that it is advisable for the community to maintain the safety of water springs, one of which cleans the area of ​​the spring, and does not dispose of waste around the springs location.