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Oman Zuas
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Astronomy Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Social Sciences Other

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Comparison of Yeast Resazurin Versus MTT Assay in vitro Methods For Determining Acute Toxicity of Halogenated Alkanes Oman Zuas
Jurnal ILMU DASAR Vol 9 No 2 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.984 KB)

Abstract

Halogenated alkanes may have potentially human health effect as a result of their persistence, bioaccumulation and toxicity after their release from environment into the food chain and water products. It is leading to increase attention for legislation aimed at prevention and great pressure to reduce the production and emission rate of halogenated alkanes. Besides many research efforts to understand the fate and (eco)toxicological effects of the halogenated alkanes. Several investigators have used animal in vivo in conventional toxicity studies of halogenated alkanes. Nevertheless, experimental by using animal testing is always time and resource demanding. Thus, it is not deemed suitable for screening of large number of potential toxicants. The main objective of this work was to investigate the comparability of yeast resazurin assay versus MTT assay for determining in vitro acute toxicity (EC50) of halogenated alkanes. The MTT assay was conducted using Chinese Hamster Ovary (CHO cell), whilst yeast strains were used in yeast resazurin assay. The study demonstrates a comparability result to which halogenated alkanes is more toxic to CHO cell than to yeast cell.
Estimasi Ketidakpastian Nilai Acuan Kobalt Dan Mangan Dalam Matriks Air Minum Kemasan Christine Elishian; Isna Komalasari; Eka Mardika Handayani; Oman Zuas; Nuryatini Hamim; Ayu Hindayani; Dyah Styarini; Eka Pratiwi
Jurnal Teknologi Vol 14 No 1 (2021): Jurnal Teknologi
Publisher : Jurnal Teknologi, Fakultas Teknologi Industri, Institut Sains & Teknologi AKPRIND Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34151/jurtek.v14i1.3542

Abstract

Ketidakpastian merupakan salah satu bagian penting dari nilai acuan suatu bahan acuan bersertifikat. Pada penelitian ini, komponen – komponen ketidakpastian pada penentuan nilai acuan kobalt (Co) dan Mangan (Mn) dalam bahan acuan matriks Air Minum Kemasan (AMK) telah berhasil diidentifikasi dan ditentukan dengan mengacu pada ISO/Guide 35:2017. Komponen yang berkontribusi pada nilai acuan Co dan Mn yaitu komponen ketidakpastian yang bersumber dari homogenitas sampel, ketidakpastian asal stabilitas jangka panjang penyimpanan dan ketidakpastian yang bersumber dari karakterisasi. Proses karakterisasi nilai acuan dilakukan dengan perbandingan dua metode yaitu ICP-MS dan ICP-OES menggunakan internal standar Ytrium dan kalibrasi eksternal. Nilai acuan logam Co dan Mn beserta ketidakpastiannya yaitu 14,8 ± 2,7 µg Kg-1 dan 41,6 ± 7,5 µg Kg-1 pada tingkat kepercayaan 95% dengan faktor cakupan 2. Adapun sumber kontributor terbesar ketidakpastian nilai acuan ditemukan berasal dari komponen stabilitas jangka panjang yaitu sebesar 1,17 µg Kg-1 (8%) dan 3,59 µg Kg-1 (9%) masing-masing untuk Co dan Mn.
Analisis Bibliometrik Penelitian Bio-Sintesis Nanopartikel Perak (NP-Ag) di Indonesia Oman Zuas; Nazarudin Nazarudin; Salprima Yudha; Umi K Yaumidin; Suripto D Yuwono; Anthoni B Aritonang
Jurnal Riset Fisika Indonesia Vol 1 No 2 (2021): Juni 2021
Publisher : Jurusan Fisika, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.691 KB) | DOI: 10.33019/jrfi.v1i2.2380

Abstract

Tulisan ini menyajikan analisis bibliometrik terhadap artikel ilmiah yang terbit dalam kurun waktu 2011-2020 terkait penelitian bio-sintesis nanopartikel perak (NP-Ag) di Indonesia. Analisi bibliometric dilakukan terhadap 55 artikel ilmiah yang diperoleh melalui pencarian di database Scopus. Hasil analisis menunjukkan bahwa penelitian dan pengembangan bio-sintesis NP-Ag di Indonesia cukup mendapat banyak perhatian yang dibuktikan oleh adanya temuan berikut. Trend pertumbuhan artikel ilmiah terkait NP-Ag diperkirakan masih akan terus mengalami kenaikan untuk beberapa tahun ke depan. Posisi pertama untuk peneliti dan institusi yang paling produktif dalam memublikasikan artikel ilmiah adalah masing-masing ditempati oleh Handayani, W., dan Universitas Indonesia (UI). Handayani, W. juga diketahui merupakan peneliti yang mempunyai jaringan kolaborasi yang terbanyak, dan untuk kolaborasi tingkat insitusi, di tempati oleh Institut Teknologi Sepuluh November (ITS). Untuk jurnal sebagai sumber artikel ilmiah yang paling menonjol ditempati oleh “Biointerface Research in Applied Chemistry”. Lebih lanjut, hasil analisis konten terhadap 55 jurnal ilmiah yang terbagi menjadi tiga klaster menunjukkan bahwa penelitian bio-sintesis dimasa yang akan datang hendaknya melibatkan peneliti dari multidisiplin ilmu dengan hasil yang lebih inovatif, dengan arah dan skala pengembangan NP-Ag yang bersifat aplikatif, serta menuju arah industrilisasi.
SIMULTANEOUS MEASUREMENT OF SYN-GAS COMPONENT (H2, CO2, CH4, AND CO) AS PRODUCT OF BIOMASS GASIFICATION BY USING VALIDATED GC-TCD METHOD Muhammad Rizky Mulyana; Oman Zuas; Harry Budiman; Sabar Simanungkalit; Nino Rinaldi
Scientific Contributions Oil and Gas Vol. 41 No. 1 (2018): SCOG
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/scog.41.1.298

Abstract

Biomass gasification has been widely known method to produce syn-gases, which can be considered as great alternative substitution for fossil fuels. However, accurate verification of the syn-gas composition requires a reliable and validated method for simultaneous measurement of H2, CO2, CH4, and CO. In order to fulfill the aforementioned requirements, which is the objective of this study; a GC-TCD method for simultaneous measurement of H2, CO2, CH4, and CO component in syn-gas has been validated in accordance to ISO/IEC 17025 and its application for real samples. For the method validation purpose, all of the performance parameters were evaluated and discussed in detail in this study. Intraday precision and interday precision were found to be acceptable having the %RSD below 0.67xCV Horwitz and below 1.0xCV Horwitz, respectively. The bias value falls within +2? for entire components, indicating that the method accuracy can be accepted. Coefficient correlation for all components was higher than 0.99, which means that the method has suffi cient linearity. LoD and LoQ for each component were found to be lower than typical syn-gas concentration range. The method roughness was also found in an acceptable level (the p-values was higher than 0.05 for allctarget components. Application of the validated method for the measurement of a real syn-gas samples indicated that the method is quite reliable to produce an accurate data and the validated methid can be used in a routine analysis. The innovation of this study, as shown by experiment results, is to evaluate the validated methods performance for simultaneous determination of syn-gas components concentration in one channel, compared to common method in which the syn-gas components were measured separatedly in a different channel. Biomass gasifi cation has been widely known method to produce syn-gases, which can be consideredas great alternative substitution for fossil fuels. However, accurate verifi cation of the syn-gas compositionrequires a reliable and validated method for simultaneous measurement of H2, CO2, CH4, and CO. Inorder to fulfi ll the aforementioned requirements, which is the objective of this study; a GC-TCD methodfor simultaneous measurement of H2, CO2, CH4, and CO component in syn-gas has been validated inaccordance to ISO/IEC 17025 and its application for real samples. For the method validation purpose,all of the performance parameters were evaluated and discussed in detail in this study. Intraday precisionand interday precision were found to be acceptable having the %RSD below 0.67xCV Horwitz and below1.0xCV Horwitz, respectively. The bias value falls within +2? for entire components, indicating that themethod accuracy can be accepted. Coeffi cient correlation for all components was higher than 0.99, whichmeans that the method has suffi cient linearity. LoD and LoQ for each component were found to be lowerthan typical syn-gas concentration range. The method roughness was also found in an acceptable level(the p-values was higher than 0.05 for allctarget components. Application of the validated method for themeasurement of a real syn-gas samples indicated that the method is quite reliable to produce an accuratedata and the validated methid can be used in a routine analysis. The innovation of this study, as shown byexperiment results, is to evaluate the validated methods performance for simultaneous determination ofsyn-gas components concentration in one channel, compared to common method in which the syn-gascomponents were measured separatedly in a different channel.Keywords: syn-gas, GC-TCD, method validation.
KETIDAKPASTIAN PENGUKURAN: EVALUASI, SUMBER-SUMBER DAN KONTRIBUSINYA DALAM PEMBUATAN BAHAN ACUAN CAMPURAN GAS (N2O DALAM MATRIKS N2) SECARA GRAVIMETRI Muhammad Rizky Mulyana; Harry Budiman; Oman Zuas; Nur Tjahyo Eka Darmayanti
JURNAL STANDARDISASI Vol 20, No 1 (2018)
Publisher : Badan Standardisasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31153/js.v20i1.599

Abstract

Gas dinitrogen monoksida (N2O) merupakan salah satu komponen gas rumah kaca dengan konsentrasi atmosferik yang terus meningkat setiap tahun, sehingga dibutuhkan upaya mitigasi yang didukung dengan metode pengukuran yang tervalidasi. Berdasarkan ISO/IEC 17025, metode pengukuran harus divalidasi unjuk kerjanya menggunakan bahan acuan yang memenuhi persyaratan, salah satunya yaitu memiliki nilai konsentrasi yang tersertifikasi beserta ketidakpastiannya. Di Indonesia, beberapa perusahaan gas specialty telah memulai upaya memproduksi bahan acuan gas sendiri untuk memenuhi kebutuhan dalam negeri. Untuk mendukung upaya tersebut, dalam studi ini akan dibahas secara rinci sumber-sumber ketidakpastian bahan acuan gas N2O dan kontribusi dari masing-masing sumber, hingga diperoleh nilai ketidakpastian akhir melalui prosedur estimasi yang akurat dan dapat diterima secara internasional berdasarkan ISO 6142. Nilai ketidakpastian diestimasi berdasarkan eksperimen aktual pembuatan 5 buah bahan acuan gas N2O dengan konsentrasi yang berbeda secara gravimetrik. Hasil ketidakpastian akhir untuk bahan acuan gas AH06018, AH06023, AH06021, AH06010, AH06020 berturut – turut adalah 2.528%, 3.490%, 4.215%, 4.785%, 5.292%. Adapun sumber ketidakpastian terbesar berasal dari fraksi mol komponen dalam parent gas, yang kontribusinya meningkat seiring bertambahnya pengenceran. Sementara itu, kontribusi ketidakpastian dari massa gas yang ditransfer mengalami penurunan dengan semakin rendahnya konsentrasi N2O. Berdasarkan hasil tersebut, disimpulkan bahwa nilai ketidakpastian konsentrasi bahan acuan meningkat seiring bertambahnya pengenceran, dan sumber ketidakpastian yang memberikan kontribusi terbesar adalah fraksi mol komponen dalam parent gas. Diharapkan hasil studi ini dapat menjadi acuan bagi produsen bahan acuan gas lokal dalam mengestimasi ketidakpastian secara tepat, serta meminimalkan kontribusi sumber-sumber ketidakpastian yang telah dibahas secara rinci.
PENERAPAN PROSEDUR SNI DALAM UJI BANDING LABORATORIUM Analisa Kadar Proksimat dalam Powdered Tonic Food Drink Oman Zuas; Nuryatini Nuryatini; Dyah Styarini
JURNAL STANDARDISASI Vol 11, No 2 (2009): Vol. 11(2) 2009
Publisher : Badan Standardisasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31153/js.v11i2.647

Abstract

The primary objective of proficiency testing (PT) is in the provision of information and support to participatinglaboratories, to enable them to monitor and improve the quality of their measurements. However, other benefitscan be obtained from PT. These include the comparison of data for a given measurement by different methods,the validation of new methods, and the provision of information for laboratories' customers and accreditationbodies. This paper reports on the subject of the participation of Puslit Kimia LIPI on PT for proximate analysis(ash, moisture, and fat content) in Powdered Tonic Food Drink (PTFD) by using SNI: 01-2891-1992. This PT wasorganized by Food and Nutrition Research Institute (FNRI)-Philippines. The PT scheme comprisses of theselection of analysis method, method verification, and analysis of proximate in PTFD sample followed byestimating its uncertainty. The final evaluation of PT by FNRI reported that Puslit Kimia LIPI has achieved asatisfactory result for proximate analysis in the PTFD sample. From this standpoint, it can be concluded that theSNI: 01-2891-1992 has shown great performance and gave comparable result to other participants from othercountries involved in this PT.
Co-Authors Aritonang, Anthoni Batahan Ayu Hindayani Christine Elishian Dyah Styarini Dyah Styarini Eka Mardika Handayani Eka Pratiwi Harry Budiman Isna Komalasari Muhammad Rizky Mulyana Muhammad Rizky Mulyana Nazarudin Nazarudin Nino Rinaldi Nur Tjahyo Eka Darmayanti Nuryatini Hamim Nuryatini Nuryatini Sabar Simanungkalit SALPRIMA YUDHA S Suripto D Yuwono Umi K Yaumidin