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All Journal HAYATI Journal of Biosciences Journal Pharmaceutical Science and Application (JPSA) Indonesian Journal of Cancer Chemoprevention
Dyaningtyas Dewi Putri Pamungkas
Department Of Pharmacology And Clinical Pharmacy, Faculty Of Pharmacy UGM
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Combination of Solanum nigrum L. Herb Ethanolic Extract and Doxorubicin Performs Synergism on T47D Breast Cancer Cells Anindyajati Anindyajati; Sarmoko Sarmoko; Dyaningtyas Dewi Pamungkas Putri; Adam Hermawan; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev1iss2pp78-84

Abstract

Leunca (Solanum nigrum L.) has been proven to possess anticancer activity on some type of cancer cells. In vitro study of solamargine found in the herb showed cytotoxic effect against several breast cancer cell lines, such as T47D and MDA-MB-31. Hence, further study on its potential as a co-chemotherapeutic agent needs to be conducted, in order to overcome resistance problem commonly found in cancer chemotherapy. This study aimed to examine the cytotoxic activity of leunca herb ethanolic extract (LEE) alone and its combination with doxorubicin. Single and combinational treatment of LEE and doxorubicin on T47D breast cancer cells were done, and their viability representing cytotoxicity were analyzed by using MTT assay to determine the IC50 value and combination index (CI) to evaluate the combinational effect. Twenty four hours-treatment of LEE alone gave cytotoxicity activity showing a dose-dependent manner with the IC50 of 47 µg/ml, while combinational treatment showed that 4 µg/ml LEE was found to be synergist with 4 nM doxorubicin on T47D cells, with the optimum CI value of 0.59. This result shows that Solanum nigrum L. is potential to be proposed as doxorubicin co-chemotherapeutic agent against breast cancer. Further study on its molecular mechanism needs to be conducted.Key words: Solanum nigrum, doxorubicin, synergist, breast cancer
Ethanolic Extract of Secang (Caesalpinia sappan L.) Wood Performs as Chemosensitizing Agent Through Apoptotic Induction on Breast Cancer MCF-7 Cells Rahmi Khamsita; Adam Hermawan; Dyaningtyas Dewi Pamungkas Putri; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 3, No 3 (2012)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev3iss3pp444-449

Abstract

Resistance to chemotherapy is believed to cause treatment failure of the patient cancer. Secang (Caesalpinia sappan L.) has been proven to possess anticancer activity on some cancer cell lines. The aimed of this study to develop ethanolic extract of secang wood (EES) as chemosensitizing agent through apoptotic induction on breast cancer MCF-7 cells. Extraction of secang was done by using maceration with 70 % ethanol. Single and combinatorial treatment of EES and doxorubicin on MCF-7 breast cancer cells were analyzed by using MTT assay to determine the IC50 value and combination index (CI) to evaluate the combinatorial effect. Apoptosis was analyzed with flowcytometry (annexin V).  EES showed a dose-dependent cytotoxicity (IC50 value of 37 µg/ml), while combinatorial treatment showed that 7 concentrations was found to be synergist with doxorubicin on MCF-7 cells. Combinatorial treatment also triggered apoptotic instead of single treatment. Based on this result, we conclude that ethanolic extract of secang wood is potential as chemosensitizing agent in breast cancer.Keywords: Caesalpinia sappan L, MCF-7 cells, doxorubicin, apoptosis.  
Synergistic Effect of Cinnamon Essential Oil (Cinnamomum burmannii) and Doxorubicin on T47D Cells Correlated with Apoptosis Induction Etyk Yunita Anjarsari; Nita Kristina; Yonika Arum Larasati; Dyaningtyas Dewi Pamungkas Putri; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 4, No 1 (2013)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev4iss1pp450-456

Abstract

Cinnamon  (Cinnamomum burmannii)  shows  anticancer activity  in  several  types  of  cancer  cells.  The aim of this study is to  observe the  cytotoxic  activity  of  cinnamon essential oil (CEO) solely and its combination with doxorubicin,  also the  ability  of  the  combination to induce apoptosis on T47D breast cancer cells. The CEO was prepared through distillation of dry cinnamon bark. Cytotoxic  assay  was performed by using MTT assay and apoptosis determination was done by using double staining with ethidium bromide and acridine orange. The result showed that CEO exhibited cytotoxic effect on T47D cells with IC50 values of 75 µg/ml.  Moreover, CEO showed synergist effect with doxorubicin. The lowest combination index (CI) with CI values of 0,37 was obtained by combination of doxorubicin-CEO  37,5  µg/ml-1,25  µM.  Treatment with CEO solely and its combination with doxorubicin showed apoptosis induction on T47D cells. The results of this  study  indicate  the potency of CEO  to  be  developed  as co-chemotherapeutic  agent  of  doxorubicin on breast cancer. Keywords:  cinnamon essential oil, doxorubicin, T47D cells, combination cytotoxic
Ethanolic Extract of Mangosteen (Garcinia mangostana) Peel Inhibits T47D and Hela Cells Line Proliferation Via Nf-қB Pathway Inhibition Erlina Rivanti; Annishfia Lailatur Rohmah; Herwandhani Putri; Prisnu Tirtanirmala; Dyaningtyas Dewi Pamungkas Putri
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev3iss2pp391-397

Abstract

Effective and selective chemoterapeutic and chemopreventive agent is needed to cure breast and cervical cancers. One of the potential natural material is mangosteen peel (Garcinia mangostana). In this study, we observed cytotoxic effect of ethanolic extract of mangosteen peel (EMP) on HeLa cells line and T47D cells line. The cytotoxic effect was determined using MTT assay.EMP showed cytotoxic effect on T47D cells and HeLa cells with IC50 values of 2.07 μg/ml and 10.58 µg/ml respectively. Molecular docking simulation was done to predict the molecular mechanism of active compund in mangosteen peel extract, α-mangostin, in NFқB pathway which is one of the potential pathway to induce cytotoxicity on T47D and HeLa cells. Docking was done using PLANTS software and the binding score between α-mangostin and proteasom is -78,12, whereas the binding score between α-mangostin and IKK is -86.84. These results showed the possiblity mechanism of mangostin peel extract containing α-mangostin inhibits IKK activation in NFқB pathway. Based on this study, we conclude that mangosteen peel extract is potential to be developed as chemopreventive agent toward cervical and breast cancers.Keywords: Mangosteen peel (Garcinia mangostana), cytotoxic, T47D cells, HeLa cells, NFқB
THE EFFECT OF VARIOUS LOCATION AND SOLVENT TO SAMBILOTO (Andrographis paniculata (Burm.f.) Nees) PHYTOCHEMICAL PROFILES Priyasana, I Putu; Nugroho, Agung Endro; Siswanto, Soni; Putri, Dyaningtyas Dewi Pamungkas; Murti, Yosi Bayu
Journal Pharmaceutical Science and Application Vol 5 No 2 (2023): Journal Pharmaceutical Science and Application
Publisher : Departement of Pharmacy, Faculty of Mathematic and Natural Science, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JPSA.2023.v05.i02.p04

Abstract

Background: Sambiloto (Andrographis paniculata (Burm.f.) Nees) is one of the traditional medicines that contain multiple phytochemical compounds. Phytochemical compounds can be influenced by growing location. Every location has its characteristic that affects the metabolic process of phytochemical compounds. Apart from that, the extraction solvent also plays an essential role in attracting compounds in sambiloto. The solvent is related to the polarity and can affect the phytochemical profile. Objective: This research aims to determine the effect of the growing location and extraction solvent of sambiloto on its phytochemical profile. Methods: Sambiloto from Denpasar (Dps), Sukoharjo (Skj), and Sleman (Slm) was extracted (1:10 w/v) using ultrasonic-assisted extraction in methanol 100% (MeOH100) and methanol 50% (MeOH50). In water solvents, extraction is carried out using the infusion method. Fingerprint analysis using HPTLC-Densitometry. The data obtained was analyzed further using principal component analysis (PCA). Results: Fingerprint analysis obtained peak data that varied in each extraction solvent. There are four major peaks in the chromatogram profile of each solvent. The peak chromatogram for each solvent also shows differences at each location. PCA analysis shows that the phytochemical content of sambiloto extract is divided into three main clusters. The distribution of the clusters is based on variations in the extraction solvent. Variations in growing location for each sambiloto also influence its phytochemical profile. However, these variations are still in the same quadrant for each solvent. Conclusion: The solvent determines more variations in the phytochemical profile of sambiloto extract than the growing location. Keywords: Sambiloto; Solvent; Location; Phytochemical; Principal Component Analysis
Cell Cycle Target Protein Induced by Galangin Treatment in Luminal Cells Confirmed by Bioinformatics Analysis Sekarini, Diyah Novi; Jati, Yohanes Surya; Zulkepli, Nur Ayunie; Putri, Dyaningtyas Dewi Pamungkas
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.969-979

Abstract

Galangin has activity modulating cell cycle arrest on luminal cancer cells and has high selectivity and low cytotoxicity for normal cells. This research intends to know galangin's prospective targets for promoting cell cycle arrest in luminal breast cancer via experimental in vitro, network pharmacology, and bioinformatics validation. In this research, MCF-7, a luminal model cell, was treated with galangin dose-dependent. Consequently, galangin exhibited a cytotoxic impact, with IC50 values of 117.86 μM. After that, SwissTargetPrediction, UALCAN, ShinyGO, and OncoLnc were used for bioinformatics validations, and Cytoscape software and the STRING website were used for computational analysis. Eight overlapping galangin target genes against luminal breast cancer were found. According to the analysis of the protein-protein interaction (PPI) network, eight hub genes-including CDK1, PLK1, TOP2A, ESR1, AURKB, NEK2, MMP9, and CA12-had the highest degree of freedom. Cell cycle regulation has been discovered to be tightly associated with overexpression of CDK1, PLK1, AURKB, and NEK2. By influencing the cell cycle, galangin inhibits the growth of luminal breast cancer, as determined by GO and KEGG enrichment analyses. In conclusion, by triggering cell cycle arrest, galangin may be used as a prospective chemotherapeutic treatment.
The Chemopreventive Potential of Diosmin and Hesperidin for COVID-19 and Its Comorbid Diseases Utomo, Rohmad Yudi; Ikawati, Muthi'; Putri, Dyaningtyas Dewi Pamungkas; Salsabila, Irfani Aura; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 11, No 3 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss3pp154-167

Abstract

The COVID-19 becomes worse with the existence of comorbid diseases such as cardiovascular diseases, metabolic syndromes, inflammation, degenerative diseases, as well as cancer. Therefore, a comprehension approach is needed to combat such comorbid conditions, not only focusing on the virus infection and replication but also directed to prevent the raising comorbid symptoms. This study analyzed the potential natural compounds, especially diosmin and hesperidin, as an anti-SARS-CoV-2 and chemopreventive agent against several COVID-19 comorbid diseases by using an in-silico method. Diosmin and hesperidin together with other natural compounds and existing viral drugs (lopinavir, nafamostat, and comastat) were docked into several proteins involved in SARS-CoV-2 infection and replication namely SARS-CoV-2 protease (PDB:6LU7), spike glycoprotein-RBD (PDB:6LXT), TMPRSS2, and PD-ACE2 (PDB:6VW1) using MOE software. The interaction properties were determined under docking score values. The result exhibited that diosmin and hesperidin performed superior interaction with all the four proteins compared to the other compounds, including the existing drugs. Moreover, under literature study, diosmin and hesperidin also elicit good chemopreventive properties against cardiovascular disorder, lung and kidney degeneration, as well as cancer development. In conclusion, diosmin and hesperidin possess high opportunity to be used for the COVID-19 and its the comorbid diseases as chemopreventive agents.Keywords: chemoprevention, COVID-19, diosmin, hesperidin, SARS-CoV-2 infection
Bioinformatics Analysis of Inhibition Activation SHP-2 by Galangal as Activating Agent of Cancer Immunotherapy Ardriyanto, Maria Indra; Rahman, Faaza Aulia; Hastuti, Hanaan Emilia Adi; Meiyanto, Edy; Kawai, Taro; Putri, Dyaningtyas Dewi Pamungkas
Indonesian Journal of Cancer Chemoprevention Vol 14, No 1 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss1pp1-11

Abstract

Interleukin 12 (IL-12) is a pro-inflammatory cytokine type 1 that has acted as a potential immunotherapy for cancer. The mechanism of IL-12 increases the activity of cytotoxic T cells and Natural Killer (NK) cells, reverse tumor-induced immunosuppression, prevent angiogenesis, and increases lymphocyte and antigen transport. Galangal is one of the natural ingredients that have biological activity as an anticancer and immunomodulator. In this research, researchers wanted to know the potential of the active compound of galangal to activate IL-12 by inhibiting the IL-12 analog, namely SHP-2. This research uses bioinformatics studies using several databases such as RCSB PDB, ChEMBL, Dr. Duke's Phytochemical and Ethnobotanical, UALCAN, OncoLnc and computational analysis using KNIME and MOE software. The SHP-2 structure used is taken from the RCSB PDB with the code 5EHR. The 10 compounds with the highest predictions of inhibiting SHP2 using KNIME were obtained, then molecular docking was performed using MOE and three compounds that had the potential to inhibit SHP-2 were Kaempferide, Galangin, and RiboflavinKeywords: cancer, computing, galangal, Interleukin 12, SHP-2.
Solanum nigrum Ethanolic Extract (SNE) Increases Cytotoxic Activity of Doxorubicin on MCF-7 Cell Putri, Dyaningtyas Dewi Pamungkas; Rivanti, Erlina; Istiaji, Raditya Prima; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 12, No 2 (2021)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev12iss2pp67-73

Abstract

Leunca (Solanum nigrum L.) is a potential source of natural anticancer agents. Solanum nigrum L. ethanolic extract (SNE) has cytotoxic activity in several cancer cell lines. We aimed to evaluate the ability of SNE to increase MCF-7 cell sensitivity to doxorubicin as a chemotherapeutic agent for breast cancer. Cell viability of SNE and its combination treatment with doxorubicin were conducted by 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) assay, and apoptosis assay was analyzed by Ethidium bromide-acridine orange method. The SNE showed a cytotoxic effect in the MCF-7 cell line with IC50 50 μg/mL. Combination treated DOX-SNE resulted in a combination index (CI) value of 0.21, indicating strong synergism SNE and doxorubicin. The SNE 25 μg/mL combined with doxorubicin 100 nM optimally induced apoptosis of MCF-7 cells. We concluded that SNE is the potential to be developed as a co-chemotherapeutic agent through apoptosis induction though the molecular mechanism need to explore.Keywords: Solanum nigrum L. herb ethanolic extract, doxorubicin, MCF-7, apoptosis.
Co-Authors Adam Hermawan Aditya Fitriasari Agung Endro Nugroho Anindyajati Anindyajati Annishfia Lailatur Rohmah Annishfia Lailatur Rohmah Ardriyanto, Maria Indra Astrid Ayu Maruti Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Endah Puspitasari Erlina Rivanti Erlina Rivanti Etyk Yunita Anjarsari Fikri Amalia Hastuti, Hanaan Emilia Adi Herwandhani Putri Herwandhani Putri Ika Nurzijah Ika Nurzijah Ikawati, Muthi' Ilham Augusta F. Jati, Yohanes Surya Kawai, Taro Muthi Ikawati Nita Kristina Nunuk Aries Nurulita Nur Ismiyati Nur Ismiyati Nur Ismiyati Prisnu Tirtanirmala Priyasana, I Putu Raditya Prima Istiaji Rahman, Faaza Aulia Rahmi Khamsita Rahmi Khamsita Rifki Febriansah Rifki Febriansyah Rifki Febriansyah Rohmad Yudi Utomo Salsabila, Irfani Aura Sarmoko Sarmoko Sekarini, Diyah Novi Siska Andrina Kusumastuti Siska Andrina Kusumastuti Siswanto, Soni Yonika Arum Larasati Yosi Bayu Murti Zulkepli, Nur Ayunie