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Comet Assay Assessment of DNA Damage in Buccal Mucosa Cells Exposed to X-Rays via Panoramic Radiography Yanuaryska, Ryna Dwi
Journal of Dentistry Indonesia Vol. 25, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Ionizing radiation (IR) presents a risk to human health via DNA damage even when administered at low doses, such as those used in panoramic radiography. Objectives: This study used the comet assay to assess DNA damage in buccal mucosa cells consequent to X-ray radiation from panoramic radiography. Methods: Twenty participants were recruited from among patients who underwent panoramic examinations at Prof. Soedomo Dental Hospital, Universitas Gadjah Mada, and divided into two groups of 10. Buccal mucosa cells were collected from all participants before exposure to IR and at 30 min or 24 h after exposure in groups 1 and 2, respectively, and subjected to a comet assay to assess DNA damage. Assay output images were analyzed using OpenComet software. Double-strand breaks (DSBs) were assessed by comparing the percentages of tail DNA in output images obtained before and after X-ray exposure. Results: A statistically significant (p=0.014) increase in the percentage of tail DNA was observed at 30 min after exposure, but not at 24 h (p=0.29). Conclusion: Panoramic X-ray radiation may induce DSBs in human buccal mucosal cells within 30 min after exposure.

Effect of β-carotene patch application on gingival crevicular fluid volume after repeated periapical radiographic exposure Faluthia Arini Puspitaningrum; Rurie Ratna Shantiningsih; Ryna Dwi Yanuaryska
Majalah Kedokteran Gigi Indonesia Vol 4, No 3 (2018): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Free radicals generated during ionization process of X-rays can damage biological tissues. Radiation exposure to gingival sulcus area will damage endothelial cells and increase permeability of blood vessels under sulcular and junctional epithelium. That inflammation will increase gingival crevicular fluid (GCF) volume. Repeated periapical radiographs often occurs due to the unfulfillment of quality assurance and leads to an increase amount of radiation dose received by the patient. Previous studies have shown that β-carotene mucoadhesive gingival patch can penetrate mucous membrane and provide protection against radiation by reducing the number of gingival epithelial cells micronuclei. The aims of this study was to observe β-carotene mucoadhesive gingival patch effect in GCF volume from patient exposed to repeated periapical radiographs. We recruited 10 participants from patients who receive repeated periapical radiographs in instalation of dentomaxillofacial radiology, Prof Soedomo dental and oral hospital Faculty of Dentistry UGM. The teeth of the subjects are divided into control and treatment group. β-carotene mucoadhesive gingival patch was applied to treatment group. GCF was collected using an absorbing paper strip before and after exposure, then measured bysliding caliper. Paired T-test showed significant differences (p<0.05) between GCF volume before and after radiographic exposure in each group. Independent T-test showed significant differences (p<0.05) of GCF volume between control and treatment group. Conclusion of this study is β-carotene mucoadhesive gingival patch significantly reduce GCF volume after repeated periapical radiographic exposure.

Panoramic and periapical radiographs utilization in Disaster Victim Identification (DVI): narrative review Vivin Nadine Ekayultania; Ryna Dwi Yanuaryska; Silviana Farrah Diba
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Vol 5 No 3 (2021): Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI)
Publisher : Ikatan Radiologi Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jrdi.v5i3.714

Objectives: The purpose of this narrative review is to discover radiographic images in panoramic and periapical radiographs that are used as identifiers and to compare the use of panoramic and periapical radiographs in identification based on DVI. Review: The databases used in this narrative review are Google Scholar, PubMed, and Science Direct. A total of 1258 search results appeared based on keywords. The search results were selected by title and abstract according to their relevance to the review topic, then results are selected again based on the inclusion and exclusion criteria. Total of 38 literatures were reviewed. This review shows radiographic identifiers used in panoramic radiographs are tooth restorations, crown, Root Canal Treatment (RCT), dental bridge, dental implants, maxillary sinus, rectilinear metal plate, orthodontic brackets, tooth anomaly, and root morphology. The radiographic identifiers used in periapical radiograph are tooth restorations, PSA, tooth anomaly, and root morphology. In this review, 53.8% of the literatures used panoramic radiograph for identification, whereas 46.2% used periapical radiograph. Conclusion: This review concluded that the most used radiographic identifier in panoramic radiograph is tooth restoration (57,1%) whereas in periapical radiograph is RCT (83,3%). Panoramic radiography were used in 53,8% of the literatures in this review, it was used more than periapical radiography.

The accuracy of extraoral periapical radiography on determining the working length in endodontic treatment: narrative review Khairin Yonni Kusumawati; Ryna Dwi Yanuaryska; Rurie Ratna Shantiningsih
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Vol 6 No 1 (2022): Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI)
Publisher : Ikatan Radiologi Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jrdi.v6i1.770

Objectives: This review article is aimed to determine the accuracy of extraoral periapical radiography as a determinant of the working length in endodontic treatment and the advantages and disadvantages of this technique. Review: Endodontic treatment is a dental treatment for damages reaching the pulp which aims to maintain the tooth. One way to succeed in endodontic treatment is to determine an accurate working length. The working length can be determined by intraoral periapical radiography examination but some people are sensitive to intraoral film placement. The placement of film on periapical radiography extraorally is needed. Conclusion: The study showed that the extraoral periapical radiography had more than 75% accuracy in determining the working length depending on angulation used. This review concludes that extraoral periapical radiography can be used in patients who can not tolerate to intraoral film placement with an accuracy that is not significantly different from that of the intraoral periapical radiography but is not meant to be replaced intraoral periapical radiography.

Fungsi pelindung tiroid dan persepsi dokter gigi terhadap penggunaannya pada teknik radiografi sefalometri dan CBCT Muhammad Yusuf Lubis; Ryna Dwi Yanuaryska; Rini Widyaningrum
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Vol 4 No 3 (2020): Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI)
Publisher : Ikatan Radiologi Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jrdi.v4i3.615

Objectives: The aims of this review is to describe thyroid shield function and to look the dentists’ perceptions considering the application in dental radiographic examination, particularly in cephalometric and Cone Beam Computed Tomography (CBCT) examination. Literature Review: The utilization of thyroid shield has been signified reduction of radiation dose about 34% in cephalometric examination and 18-40.1% in CBCT. The absence of universal guidelines has led to minimal perception of dentists on the importance of using thyroid shield as indicated by the low utilization of thyroid shield among patients. This low perception can be improved through adequate education and applied training in the use of thyroid shield. Conclusion: Dentists’ perceptions regarding the application of thyroid shield need to be improved so that the application of thyroid shield can be increased in order to protect patients from the risk of dental radiation exposure.

Perbandingan sudut antegonial dan kedalaman antegonial pada radiograf panoramik antara pria dan Wanita: Studi Observasional Purba, Theresia Alfi; Widyaningrum, Rini; Mudjosemedi, Munakhir; Yanuaryska, Ryna Dwi
Padjadjaran Journal of Dental Researchers and Students Vol 7, No 3 (2023): Oktober 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v7i3.48028

ABSTRAKPendahuluan: Identifikasi individu dapat dilakukan dengan menggunakan metode identifikasi sekunder, salah satunya berupa determinasi jenis kelamin menggunakan radiograf. Identifikasi jenis kelamin dapat dilakukan dengan mengamati morfologi mandibula yang merupakan tulang terkuat dan terbesar di wajah manusia. Perbedaan mandibula antara pria dan wanita pada regio antegonial mandibula dapat diamati serta diukur pada radiograf panoramik. Penelitian ini bertujuan untuk mengetahui perbedaan sudut dan kedalaman antegonial antara pria dan wanita serta untuk menentukan perbedaan sudut dan kedalaman antegonial antara sisi kiri dan kanan pada radiografi panoramik. Metode: Sampel penelitian terdiri dari 80 radiograf panoramik dari 40 pasien pria dan 40 wanita berusia 20-60 tahun. Pengukuran sudut antegonial dan kedalaman antegonial dilakukan secara digital pada radiografi panoramik. Independent sample t-test digunakan untuk mengetahui apakah ada perbedaan diantara kedua variabel yang dibandingkan antara pria dan wanita sekaligus untuk mengetahui perbedaan antara pengukuran sisi kanan dan kiri. Hasil: Rerata sudut antegonial pada kelompok pria adalah 155,36° ± 5,85 sedangkan pada kelompok wanita adalah 159,07° ± 5,25. Rerata kedalaman antegonial pada kelompok pria adalah 2,07 ± 0,69 mm dan pada kelompok wanita adalah 1,75 ± 0,59 mm. Hasil independent sample t-test menunjukkan perbedaan yang signifikan (p<0,05) pada sudut antegonial dan kedalaman antegonial antara kelompok pria dan wanita, serta tidak ada perbedaan yang signifikan pada sudut maupun kedalaman antegonial antara sisi kiri dan kanan (p>0,05). Simpulan: Terdapat perbedaan sudut dan kedalaman antegonial antara pria dan wanita, serta tidak terdapat perbedaan yang signifikan pada sudut dan kedalaman antegonial antara sisi kiri dan kanan pada radiografi panoramik.KATA KUNCI: Radiograf, panoramik, mandibula, sudut antegonial, kedalaman antegonialComparing antegonial angle and antegonial depth on panoramic radiographs between men and women: observational studyABSTRACTIntroduction: Personal identity can be determined using secondary identification methods such as sex estimation using radiograph. Mandibular morphology can be used for estimating sex. The mandible, as the largest and most robust bone in the human facial skeleton, provides valuable information for sex estimation. The antegonial region indicates sexual dimorphism in the mandible, which can be visually assessed on panoramic radiographs. This study aims to investigate potential differences in antegonial angles and depths between males and females, in addition to identify any differences in antegonial angle and depth on panoramic radiographs between the left and right sides. Methods: The study sample included 80 panoramic radiographs taken from 40 male and 40 female patients aged 20-60 years. The antegonial angle and depth were measured digitally on panoramic radiographs. The collected data was analyzed using an independent sample t-test. Results: In the male group, the mean antegonial angle was 155.36°±5.85, while in the female group, it was 159.07°±5.25. The mean value of antegonial depth in the male group was 2.07±0.69 mm and in the female group it was 1.75±0.59 mm. Independent sample t-test reveals a significant difference in antegonial angle and depth between the male and female groups (p<0.05) and no significant difference in both measurement between the left and right sides (p>0.05). Conclusion: There are differences in antegonial angles and depths between men and women, and no significant difference in antegonial angles and depths between the left and right sides on panoramic radiographs.KEY WORDS: radiograph, panoramic, mandible, antegonial angle, antegonial depth

Evaluasi tingkat pengetahuan mahasiswa Fakultas Kedokteran Gigi Universitas Gadjah Mada pada ilmu penyakit mulut dan radiologi kedokteran gigi Herwita, Alifah Nasywa Nur; Yanuaryska, Ryna Dwi; Naritasari, Fimma
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 10, No 3 (2024)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.100571

Dokter gigi memerlukan pengetahuan yang memadai untuk merawat pasien. Pendidikan jenjang sarjana merupakan bagian dari proses belajar yang tidak terpisahkan bagi dokter gigi. Ilmu Penyakit Mulut (IPM) dan Radiologi Kedokteran Gigi (RKG) merupakan dua bidang ilmu yang membutuhkan pengetahuan mendalam dan berperan penting dalam hal diagnosis dan manajemen penyakit pada pasien. Berdasarkan Standar Kompetensi Dokter Gigi Indonesia,terdapat kompetensi minimal yang harus dicapai oleh mahasiswa. Terdapat lima jenis kompetensi pada bidang IPM dan dua jenis kompetensi pada bidang RKG. Tujuan penelitian ini adalah mengetahui tingkat pengetahuan mahasiswa dan perbandingannya antar jenis kompetensi pada masing-masing bidang ilmu, yaitu IPM dan RKG. Penelitian dilakukan pada mahasiswa angkatan 2021 program studi S1 Kedokteran Gigi FKG UGM. Terdapat 142responden yang memenuhi kriteria inklusi dan eksklusi. Enam pertanyaan IPM dan sepuluh pertanyaan RKG yang memenuhi syarat valid dan reliabel digunakan untuk mengukur pengetahuan responden terhadap dua kompetensi IPM yaitu ‘anamnesis’ dan ‘Komunikasi, Informasi, dan Edukasi (KIE)’, serta dua kompetensi RKG yaitu ‘kemampuan interpretasi’ dan ‘keterampilan prosedural’. Hasil uji Mann-Whitney U pada bidang IPM dan RKG menunjukkannilai p<0,05. Hasil penelitian menunjukkan bahwa secara statistik terdapat variasi tingkat pengetahuan mahasiswa pada bidang IPM dan RKG. Selain itu, juga terdapat perbedaan tingkat pengetahuan antar jenis kompetensi pada bidang ilmu yang sama. Pada bidang IPM, terdapat perbedaan tingkat pengetahuan antara kompetensi ‘anamnesis’ dengan ‘KIE’. Pada bidang RKG, terdapat perbedaan tingkat pengetahuan antara ‘kemampuan interpretasi’ dengan ‘keterampilan prosedural’.

Deteksi karies proksimal menggunakan radiografi bitewing dan near-infrared light transillumination Nugroho, Lathifa Dewanti; Yanuaryska, Ryna Dwi; Widyaningrum, Rini
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 9, No 3 (2023)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.100770

Radiografi bitewing merupakan metode standar untuk deteksi karies proksimal karena dapat mencitrakan mahkota gigi dari permukaan distal kaninus hingga distal permukaan molar paling posterior tanpa tumpang tindih. Pemanfaatan sinar-X di bidang kedokteran gigi menerapkan prinsip as low as reasonably achievable (ALARA) untuk mengurangi efek radiasi. Near-Infrared Light Transillumination (NILT) merupakan metode deteksi karies tanpa menggunakanradiasi pengion sehingga dapat dijadikan alternatif untuk mengatasi permasalahan tersebut. Narrative review ini ditujukan untuk mendeskripsikan kelebihan dan kekurangan antara teknik intraoral bitewing dengan NILT beserta perbedaan hasil deteksi karies proksimal dari masing-masing teknik tersebut. Pencarian literatur pada narrative review ini menggunakan Google Scholar, ScienceDirect, dan Pubmed dengan kriteria inklusi yaitu artikel berjenis original/research article, case report, dan textbook ilmiah, serta artikel berbahasa Inggris maupun Indonesia yangditerbitkan tahun 2010-2021. Kriteria eksklusi berupa review article, artikel yang menunjukkan duplikasi, artikel yang tidak dapat diakses secara utuh, serta artikel tanpa metode penelitian. Total literatur yang dikaji sebanyak 29 artikel. Hasil review menunjukkan bahwa NILT merupakan metode deteksi karies proksimal tanpa disertai risiko radiasi sehingga tidak memberikan efek berbahaya pada tubuh sehingga penggunaannya dapat diulangi sesuai kebutuhan. Kelebihan lainnya, NILT lebih sensitif daripada radiograf untuk mendeteksi jaringan keras gigi yang mengalami demineralisasi pada fase awal. Nilai sensitivitas dan spesifisitas NILT lebih tinggi daripada radiografi bitewing sehingga dapat dijadikan alternatif pemeriksaan radiografi. Meskipun demikian, NILT tidak dapat mencitrakan karies yang telah meluas hingga pulpa karena NILT tidak memiliki daya tembus sebesar sinar-X, sehingga radiografi bitewing masihmerupakan standar pemeriksaan untuk deteksi karies proksimal.

The effect of video modeling and tell-show-do on anxiety during dental radiographic examinations among children Tania, Laura; Yanuaryska, Ryna Dwi; Wardani, Putri Kusuma
Majalah Kedokteran Gigi Indonesia Vol 11, No 1 (2025): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.97994

Dental procedure anxiety refers to the fear experienced before and during dental treatment. To reduce anxiety levels in children undergoing radiographic examinations, non-pharmacological behavioral management techniques such as tell-show-do and modeling can be employed. This study aimed to compare the effectiveness of video modeling and the tell-show-do technique in reducing anxiety in children aged 5–7 years undergoing dental radiographic examinations at RSGM Prof. Soedomo, UGM. This quantitative study used a pretest–posttest control group design. The participants were children aged 5–7 years who underwent radiographic examinations at the Radiology Department of RSGM Prof. Soedomo, UGM, between September and December 2023. The sample was divided into three groups: Group A received the video modeling intervention, Group B received the tell-show-do intervention, and Group C served as the control group. Data were collected using the Modified Dental Anxiety Scale (MDAS) questionnaire and physiological anxiety measurements (blood pressure and pulse rate) using an Omron HEM-6161 digital tensiometer. MDAS scores were analyzed using the Kruskal-Wallis test, while blood pressure and pulse rate were analyzed using one-way ANOVA. A total of 146 participants were included. Significant differences were observed in MDAS scores, blood pressure, and pulse rate from pretest to posttest among all groups (p < 0.05). The tell-show-do group showed the greatest reduction in anxiety (3.74 ± 2.62), followed by the video modeling group (3.33 ± 2.30), and the control group (0.92 ± 2.33). Both the tell-show-do technique and video modeling are effective in reducing dental anxiety in children aged 5–7 years undergoing radiographic examinations at RSGM Prof. Soedomo, UGM.

Comparison of infection control practices in dental radiographic examination during the COVID-19 pandemic among various health facilities in Yogyakarta, Indonesia Dewangga, Mella Anggia Puspa; Shantiningsih, Rurie Ratna; Widyaningrum, Rini; Yanuaryska, Ryna Dwi
Majalah Kedokteran Gigi Indonesia Vol 10, No 3 (2024): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.95895

Dental radiography services were at high risk of becoming sites for cross-infection during the COVID-19 pandemic. To minimize the spread of COVID-19, infection control adaptations were implemented in dental radiography services across various healthcare facilities, including Type A, B, C, and D hospitals, as well as clinical laboratories. This study aims to determine the differences in infection control practices of dental radiographic examinations during the COVID-19 pandemic at various health facilities. This cross-sectional study involved 42 dental radiographers who worked in Yogyakarta. Control infections were measured using the electronic questionnaire with 27 closed-ended questions. The data were analyzed statistically using the Kruskal-Wallis test, and descriptive analysis was continued by categorizing the control infection into high, medium, and low levels. The validity and reliability test showed that 18 questionnaire items were valid and reliable. The statistical test showed a p-value of 0.672 (p > 0.05) for the staff infection control and 0.147 (p > 0.05) for the room infection control. This study found no significant differences in infection control practices during dental radiographic examinations across various healthcare facilities during the COVID-19 pandemic. All facilities implemented high levels of infection control, with clinical laboratories showing the highest compliance. The study suggests increasing the number of questionnaire items related to room infection control and expanding the study’s scope in future research to improve accuracy and represent a broader population.

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