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EFEKTIVITAS BERBAGAI KONSENTRASI NEMATODA ENTOMOPATOGEN (Steinernema sp) TERHADAP MORTALITAS LARVA Spodoptera exiqua Hubner Ria, Kamariah; Natsir, Burhanuddin; Panggeso, Johanis
AGROTEKBIS Vol 1, No 1 (2013)
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Spodoptera exiqua is known as onion caterpillar, it attacks often reduces onion production.  This insect could even cause harvest failure, and chemicals in high doses and spraying is often used to control on its spread. This action can negatively impact to environment as well the users. To reduce the negative impact of the use of chemical insecticides is necessary to find an alternative strategy. It is known that several biological agents could serve as biopesticides such as  entomopathogenic nematodes Steinernema sp. This study was aimed to determine effective concentration of entomopathogenic nematodes Steinernema sp. to control S. exiqua growth.  Nematode used in this study was Infective juvenile (IJ) instar II. There were 6 different nematodes concentrations as treatments and each treatment was repeated 4 times in a Completely Randomized Design (CRD).  The treatments were 0 IJ, 200 IJ, 400 IJ, 600 IJ, 800 IJ, and 1000 IJ of nematodes, mixed in 2 ml of distilled water, respectively.  The results showed that the concentration of nematodes 600 JI is effective to kill 83.4% larvae of S. exiqua within 6 days. This finding clearly shows that nematode is able to reduce larvae S.exiqu and expecting a positive impact to onion production.
Uji Ketahanan Beberapa Varietas Jagung (Zea mays L.) Terhadap Intensitas Serangan Penyakit Bulai (Peronosclerospora maydis) J, Pajrin; Panggeso, Johanis; I, Rosmini
AGROTEKBIS Vol 1, No 2 (2013)
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The research was conducted in the Soulowe of village, District Biromaru, Sigi. Experimental design used was Randomized Block Design (RBD) with 3 (three) treatments and 5 (five) replications, in order to obtain 15 units of the experiment.  Each treatment is based on the differences in varieties. These varieties are varieties Bonanza, Paramita and Kumala. The variables measured were the intensity of the attacks and the rate of infection diseases. The first observation made at the time the plant was 14 days after planting, subsequent observations made at intervals of once a week as much as 7 times. The results of this study showed that the difference in response of resistance varieties Bonanza, Paramita and Kumala against downy mildew caused by the fungus Peronosclerospora maydis. The highest attack intensity was found in varieties Bonanza followed by Kumala varieties, while the lowest was found in varieties Paramita. The highest disease infection rate was varietas Bonanza, then followed by Kumala varietas, and then Paramita varieties that have the lowest disease infection rate.
Uji Antagonisme Trichoderma sp. Terhadap Jamur Patogen Alternaria porri Penyebab Penyakit Bercak Ungu Pada Bawang Merah Secara In-Vitro Muksin, Rusdam; I, Rosmini; Panggeso, Johanis
AGROTEKBIS Vol 1, No 2 (2013)
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The objective of this study is to know the ability the fungus Trichoderma sp. as antagonistic to fungal pathogens Alternaria porri (EII. Cif.) on shallot. This study was conducted in the Laboratory of plant pests and Disease Department (HPT), Agriculture faculty  of Tadulako University, Palu in Plant Central Sulawesi. This study used the isolation and identification of fungal pathogen A.Porri on onion and antagonism test of Trichoderma sp test against mold growth A.porri, with 2 treatments and 5 replications so that there were 10 experimental units. Local isolates of Trichoderma sp. Used in this experimen were derived from two institusion the Laboratory of plant pests and Diseases Faculty of Agriculture University of Tadulako and BPTP Biromaru. The result of this stady indikate that bolt of lokal isolate Trichoderma sp. quite effective in suppressing the growth fungi A.porri EII. Cif. disease-causing purple spots on the onion crop in-vitro, but percentage of inhibition bolth of isolate ware not significantly different at 5% level test results.
PENGARUH UMUR BIAKAN CENDAWAN Aspergillus sp. ISOLAT LOKAL PALOLO TERHADAP MORTALITAS Helopeltis spp. (HEMIPTERA : MIRIDAE) Pasaru, Remalia Bulo; Panggeso, Johanis; Khasanah, Nur
AGROTEKBIS Vol 5, No 2 (2017)
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This research aims to determine the effect of the amount of spores in the age difference cultured fungus Aspergillus sp. local isolates palolo on mortality Helopeltis spp. This research    used experimental methods of design are collated in a completely randomized design (CRD), which is testing the amount spores in each respective age culture 3, 5, 7, and 9 days, and the dose of spores in each age culture from isolates local palolo Aspergillus sp. on the death of test insects Helopeltis spp. The research was carried on in the Laboratory of Plant Pests and Diseases, Faculty of Agriculture, University of Tadulako, from May 2015 to July 2015. The results showed that in treatment P4 (age cultures of 9 days) the amount spores is more that 8.748 x 106sel and has effective power better in a deadly test insects Helopeltis spp. compared with the treatment of breeding age 3 days, 5 days, and 7 days. Key Words: Aspergillus sp., Cocoa (Theobromae cacao), Helopeltis spp.
UJI KETAHANAN BEBERAPA KLON KAKAO (Theobromae cacao L.) TERHADAP PENYAKIT BUSUK BUAH (Phytophthorah palmivora butl) Aminullah, Moh. Fajri S.; Panggeso, Johanis; Rosmini, Rosmini
AGROTEKBIS Vol 5, No 4 (2017)
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Cacao is one of the prioritized commodities of crop plantations in Central Sulawesi. However, the main obstacle faced in the cultivation of cocoa plants is plant pest organisms (OPT). One of the most frequently OPT encountered is black pod rot caused by Phytophtorah palmivora fungus. Based on the above description, this study aimed to determine the resilience of some cacao clones on the development of P. palmivora. Cocoa fruit samples of six different clones were taken from people plantations in Uenuni village, Palolo sub district of Sigi Regency, and brought to the Laboratory of Phytopathology Plant where their resistance to black pod rot disease was tested using a Detached Pod method. The six clones used included Local II (Criolo), M01, Lokal III (Mulia), CRD 60, Local I (Forestero), and Local IV (Hibrida). This study used a Completely Randomized design (CRD) with P. palmivora as a factor. The fungus was inoculated to the fruits of the six cacao clones. An experimental unit consisted of three pieces of cocoa for each clone tested and replicatedthree times.  Observations were made on the area size of spots on the fruit’ssurface and itsincreasingsizeper day for seven days. After seven days, the largest spot area of 350.79 cm2 was found in the Lokal III (Mulia) clone with the fastest rate of increasing spot size of 58.11 cm2 per day in average and with 0.63 cm thickness of the outer skin and with 0.43 cm thickness of the inner skin suggesting that this clone is very prone to P. palmivora attack. The lowest spot area found in the CRD 60 clone was 12.86cm2 with the rate of increasing spot size 1.91 cm2per day in average, and with 1.03 cm thickness of the outer skin and with 0.77 cm thickness of the inner skin suggesting that this clone belong to a group resistant to P. palmivora attack. Keywords: Cacao clone and Fungi P. Palmivora.
EFEKTIFITAS EKSTRAK RIMPANG LENGKUAS DALAM MENGHAMBAT AKTIFITAS CENDAWAN Oncobasidium theobremae SECARA In-vitro S, Ismail Suaib.; Lakani, Irwan; Panggeso, Johanis
AGROTEKBIS Vol 4, No 5 (2016)
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Vascular streak dieback disease caused by the fungus Oncobasidium theobremae is one of the important diseases of the cocoa crop. Galangal potential as a botanical pesticides that have various properties of them as anti-fungal and antibacterial. This study aims to determine the effectiveness of ginger rhizome extract in suppressing the activity of growing fungus O. theobremae. Implementation of the study began in August 2015 to February 2016 is located in the Laboratory of Plant Diseases, Faculty of Agriculture unit UNTAD sampling sites Salubomba disease in the village, District of South Banawa, Donggala, Central Sulawesi. This study uses galangal rhizome extract mixed with a concentration of 0.25%, 0.50% and 0.75% into the PDA (Potato Dextrose Agar). The results of the study during the week of observation showed that the highest concentration (0.75%) were able to inhibit the development of fungus O. theobremae with a percentage of 38.77%. Key Words : Ginger extract , concentration, Cocoa, and O. theobremae. 
EFIKASI EKSTRAK DAUN SIRIH TERHADAP Alternaria porri PENYEBAB PENYAKIT BERCAK UNGU PADA BAWANG MERAH SECARA In vitro Fahrun, Muh.; Panggeso, Johanis; Rosmini, Rosmini
AGROTEKBIS Vol 6, No 1 (2018)
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Bercak ungu disebabkan oleh jamur A. porri adalah penyakit penting yang menyerang pertanaman bawang merah dan menyebabkan kerugian dibeberapa sentra produksi di Indonesia. Pengendalian penyakit bercak ungu oleh petani masih menggunakan fungisida kimia sehingga diperlukan alternatif pengendalian dengan fungisida nabati. Salah satu fungisida nabati ialah daun sirih. Daun sirih memiliki sifat anti cendawan, dan anti oksidan dengan komponen minyak atsiri yaitu eugenol 63,39%, acetyleugenol 14,05%. Tujuan penelitian ini untuk menentukan efektivitas ekstrak daun sirih dalam menghambat pertumbuhan jamur A. porri penyebab penyakit bercak ungu pada bawang merah. Pelaksanaan penelitian di Laboratorium Jurusan Hama dan Penyakit Tumbuhan (HPT) Fakultas Pertanian Universitas Tadulako, Sulawesi Tengah. Palu. Penelitian dimulai pada bulan Januari sampai September 2016. Metode uji yaitu masing-masing konsentrasi ekstrak daun sirih di tuang sebanyak 2 ml ke dalam medium PDA. Setelah memadat, A. porri diambil menggunakan jarum ose dan diletakkan tepat di bagian tengah. Hasil penelitian menunjukkan, Ekstrak daun sirih efektif menghambat pertumbuhan A. porri, konsentrasi tertinggi dalam menghambat pertumbuhan A. porri adalah 8%. Hasil analisis regresi konsentrasi ekstrak daun sirih terhadap persentase penghambatan A. porri menunjukkan hubungan korelasi sangat    kuat (0,904). Kata kunci: Alternaria porri, Ekstrak, Sirih (Piper betle L.).
EFIKASI EKSTRAK DAUN SIRIH TERHADAP Alternaria porri PENYEBAB PENYAKIT BERCAK UNGU PADA BAWANG MERAH SECARA In vitro Fahrun, Muh.; Panggeso, Johanis; Rosmini, Rosmini
AGROTEKBIS Vol 6, No 6 (2018)
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Purple spot caused by the fungus A. porri is an important disease that attacks the shallot crop and causing loss yield in several production centers in Indonesia. Purple spot disease control still use chemical fungicides so it is necessary to control alternative vegetable fungicides. One is the betel leaf vegetable fungicides. Betel leaf have anti fungus characteristic, and anti-oxidants with essential oil component is eugenol 63.39%, 14.05% acetyleugenol. The purpose of this research to determine the effectiveness of betel leaf extract in inhibiting the growth of disease-causing fungus A. porri purple spots on shallots. Implementation of research at the Laboratory of Plant Pests and Diseases (HPT) Faculty of Agriculture, University Tadulako, Central Sulawesi. Palu. The research began in January to September 2016. The test method respectively betel leaf extract concentration in 2 ml pour into PDA medium. Once solidified, A. porri taken using a ose needle and placed right in the middle. The results showed that the betel leaf extract effectively inhibits the growth of A. porri, the highest concentration in inhibiting A. porri growth is 8%. The result of regression analysis of betel leaf extract concentration toward percentage of inhibition A. porri showed very strong correlation relationship (0,904).
PENGARUH APLIKASI Trichoderma sp. TERHADAP LAYU BAKTERI Ralstonia solanacearum PADA TANAMAN PISANG Trisnawati, Eka; Panggeso, Johanis; Asrul, Asrul
AGROTEKBIS Vol 7, No 2 (2019)
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Banana plant (Musa paradisiacal L.) is one of Indonesia native plants currently facing a serious problem of bacterial wilt disease caused by Ralstonia solanacearum bacteria. The purpose of this research was to determine the effect of Trichoderma sp. application at different rates to controll the bacterial. This research was conducted in the Plant Disease Laboratory and in the Agricultural Faculty of Agriculture, Tadulako University from April to September 2017. Banana seeds used were four month old kepok. This study used a Completely Randomized Design (CRD) with four rates of Trichoderma sp. i.e. control (T0), 20 g (T1), 40 g (T2), and 60 g (T3). Each treatment was replicated five times. The results of this study showed that the fastest incubation period occurred in T0 treatment (7 days) compared to the lowest T3 (25 days). The T3 treatment was able to reduce the bacterial wilting attack by 10-20%.
Uji Antagonisme Trichoderma sp. Terhadap Jamur Patogen Alternaria porri Penyebab Penyakit Bercak Ungu Pada Bawang Merah Secara In-Vitro Muksin, Rusdam; I, Rosmini; Panggeso, Johanis
AGROTEKBIS Vol 1, No 2 (2013)
Publisher : AGROTEKBIS

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Abstract

The objective of this study is to know the ability the fungus Trichoderma sp. as antagonistic to fungal pathogens Alternaria porri (EII. Cif.) on shallot. This study was conducted in the Laboratory of plant pests and Disease Department (HPT), Agriculture faculty  of Tadulako University, Palu in Plant Central Sulawesi. This study used the isolation and identification of fungal pathogen A.Porri on onion and antagonism test of Trichoderma sp test against mold growth A.porri, with 2 treatments and 5 replications so that there were 10 experimental units. Local isolates of Trichoderma sp. Used in this experimen were derived from two institusion the Laboratory of plant pests and Diseases Faculty of Agriculture University of Tadulako and BPTP Biromaru. The result of this stady indikate that bolt of lokal isolate Trichoderma sp. quite effective in suppressing the growth fungi A.porri EII. Cif. disease-causing purple spots on the onion crop in-vitro, but percentage of inhibition bolth of isolate ware not significantly different at 5% level test results.