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All Journal Indonesian Journal of Biotechnology The Indonesian Biomedical Journal
Dewi Kartikawati Paramita
Staf pengajar Bagian Histologi dan Biologi Sel FK UGM
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Public Health

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Evaluation of synthetic‐gene recombinant LipL32 antigen for IgM ELISA detection of Leptospira infection Widiastuti, Dyah; Paramita, Dewi Kartikawati; Murwanti, Retno; Kusrini, Ina; Wijayanti, Nastiti; Haryanto, Aris
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.111102

Abstract

Leptospirosis presents with nonspecific clinical features and requires time‐consuming laboratory tests for gold standard diagnosis. This study aims to design and characterize the recombinant LipL32 from synthetic gene and assess its performance as an antigen for detecting leptospirosis. The antigen was developed by cloning the LipL32 gene conserved portion of Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi. The immunoinformatic was used to characterize the developed rLipL32. Western blot results using anti‐histidine revealed a band of rLipL32 protein at ~40 kDa. Subsequently, it was used to examine the IgM antibody on human sera by using ELISA. The IgM‐LipL32 ELISA was evaluated using 67‐positive and 25‐negative sera and compared with a commercial ELISA. With a cut‐off value of 0.8, it showed 85.7% sensitivity, 83.3% specificity, a 48% positive prediction value (PPV), and 97% negative prediction value (NPV), indicating modest performance compared to existing commercial kits. The rLipL32 is a potential antigen for detecting IgM using ELISA; however, for use in low incidence areas, a confirmation test is crucial.
Epstein–Barr Virus Latent Membrane Protein-1 (EBV LMP-1) in Nasopharyngeal Carcinoma: Immune Correlates and Potential as A Clinical Outcome Biomarker Fachiroh, Jajah; Ramadhani, Fitriya; Hertanto, Nayaka Bagus Wahyu Agung; Paramita, Dewi Kartikawati
The Indonesian Biomedical Journal Vol 18, No 1 (2026)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v18i1.3954

Abstract

In endemic populations, nasopharyngeal carcinoma (NPC) is associated with epstein-barr virus (EBV) infection, with latent membrane-1 (LMP-1) playing a major role as an oncoprotein. Despite this well-established biological role, the clinical use of LMP-1 remains limited; therefore this review aimed to discuss the potential use of LMP-1 as clinical biomarker. Based on systematic searching results in two major biomedical journal databases, in this review, only a small number of studies that evaluated LMP-1 as a clinical outcome. Studies examining the relationship between LMP-1 and its related biomarkers in clinical samples were particularly scarce. By mapping the existing literature, this scoping review highlights mechanistic linking of LMP-1 to specific biomarker, such as interferon gamma (IFN-γ), leukemia inhibitory factor (LIF), chemokine (C-X-C motif) ligand 9 (CXCL9), programmed cell death ligand-1 (PDL-1), and had a positive regulatory loop with EBV-encoded small RNA (EBERs) serving to amplify inflammatory signals that facilitates NPC progression. A clear gap between evidence mechanism of LMP-1 and clinical research practice was observed. This may related to several reasons, including low detectability, a heterogeneous expression in tumor tissue; hence shifted into surrogated biomarkers that reflected LMP-1 signalling than the protein itself. Future studies should focus on combining LMP-1 with related inflammatory or immune markers, and conducting well-designed clinical studies to better define the potential role of LMP-1 within clinically relevant biomarker strategies for NPC.KEYWORDS: oncoprotein, stage, malignancy of nasopharynx, prognosis, survival, inflammation 
Co-Authors Aris Haryanto Dyah Widiastuti Hertanto, Nayaka Bagus Wahyu Agung Ina Kusrini Jajah Fachiroh, Jajah Nastiti Wijayanti Ramadhani, Fitriya Retno Murwanti