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All Journal Indonesian Journal of Biotechnology The Indonesian Biomedical Journal Majalah Patologi Indonesia
Dewi Kartikawati Paramita
Staf pengajar Bagian Histologi dan Biologi Sel FK UGM
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Public Health

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Evaluation of synthetic‐gene recombinant LipL32 antigen for IgM ELISA detection of Leptospira infection Widiastuti, Dyah; Paramita, Dewi Kartikawati; Murwanti, Retno; Kusrini, Ina; Wijayanti, Nastiti; Haryanto, Aris
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.111102

Abstract

Leptospirosis presents with nonspecific clinical features and requires time‐consuming laboratory tests for gold standard diagnosis. This study aims to design and characterize the recombinant LipL32 from synthetic gene and assess its performance as an antigen for detecting leptospirosis. The antigen was developed by cloning the LipL32 gene conserved portion of Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi. The immunoinformatic was used to characterize the developed rLipL32. Western blot results using anti‐histidine revealed a band of rLipL32 protein at ~40 kDa. Subsequently, it was used to examine the IgM antibody on human sera by using ELISA. The IgM‐LipL32 ELISA was evaluated using 67‐positive and 25‐negative sera and compared with a commercial ELISA. With a cut‐off value of 0.8, it showed 85.7% sensitivity, 83.3% specificity, a 48% positive prediction value (PPV), and 97% negative prediction value (NPV), indicating modest performance compared to existing commercial kits. The rLipL32 is a potential antigen for detecting IgM using ELISA; however, for use in low incidence areas, a confirmation test is crucial.
Epstein–Barr Virus Latent Membrane Protein-1 (EBV LMP-1) in Nasopharyngeal Carcinoma: Immune Correlates and Potential as A Clinical Outcome Biomarker Fachiroh, Jajah; Ramadhani, Fitriya; Hertanto, Nayaka Bagus Wahyu Agung; Paramita, Dewi Kartikawati
The Indonesian Biomedical Journal Vol 18, No 1 (2026)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v18i1.3954

Abstract

In endemic populations, nasopharyngeal carcinoma (NPC) is associated with epstein-barr virus (EBV) infection, with latent membrane-1 (LMP-1) playing a major role as an oncoprotein. Despite this well-established biological role, the clinical use of LMP-1 remains limited; therefore this review aimed to discuss the potential use of LMP-1 as clinical biomarker. Based on systematic searching results in two major biomedical journal databases, in this review, only a small number of studies that evaluated LMP-1 as a clinical outcome. Studies examining the relationship between LMP-1 and its related biomarkers in clinical samples were particularly scarce. By mapping the existing literature, this scoping review highlights mechanistic linking of LMP-1 to specific biomarker, such as interferon gamma (IFN-γ), leukemia inhibitory factor (LIF), chemokine (C-X-C motif) ligand 9 (CXCL9), programmed cell death ligand-1 (PDL-1), and had a positive regulatory loop with EBV-encoded small RNA (EBERs) serving to amplify inflammatory signals that facilitates NPC progression. A clear gap between evidence mechanism of LMP-1 and clinical research practice was observed. This may related to several reasons, including low detectability, a heterogeneous expression in tumor tissue; hence shifted into surrogated biomarkers that reflected LMP-1 signalling than the protein itself. Future studies should focus on combining LMP-1 with related inflammatory or immune markers, and conducting well-designed clinical studies to better define the potential role of LMP-1 within clinically relevant biomarker strategies for NPC.KEYWORDS: oncoprotein, stage, malignancy of nasopharynx, prognosis, survival, inflammation 
Ekspresi EBER pada Berbagai Tipe Histopatologi Karsinoma Nasofaring Anita, Kenty Wantri; Putranto, Bambang Endro; Prasetyo, Awal; Paramita, Dewi Kartikawati
Majalah Patologi Indonesia Vol. 24 No. 1, Januari 2015
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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Abstract

ABSTRAK Latar belakang Etiologi kanker nasofaring (KNF) sudah terbukti merupakan interaksi dari infeksi Epstein-Barr Virus (EBV), paparan lingkungan dan kerentanan genetik. Infeksi EBV mendominasi etiopatogenesis KNF terutama untuk tipe WHO-3. Di Indonesia, saat ini belum ada publikasi tentang ekspresi Epstein-Barr-virus-encoded small nuclear RNA (EBER) yang dikaitkan dengan klasifikasi histopatologi KNF. Tujuan penelitian ini menganalisis ekspresi EBER pada berbagai tipe histopatologi KNF untuk menambah informasi dalam diagnosis etiologi KNF, sehingga bermanfaat dalam strategi terapi. Metode Penelitian dengan desain cross sectional ini menggunakan sampel biopsi nasofaring yang didiagnosis sebagai KNF antara tahun 2009 sampai 2012 di Laboratorium Patologi Anatomik Fakultas Kedokteran Universitas Diponegoro/RSUP. Dr. Kariadi dan RS. St. Elisabeth Semarang. Sediaan dievaluasi ulang dan dilakukan in situ hybridization dengan probe fluorescein-conjugated peptide nucleic acid (PNA) pada jaringan blok parafin. Hasil Sebanyak 29 dari 31 kasus (93,5%) KNF tipe WHO-3 mengeskpresikan EBER positif dan terdapat perbedaaan bermakna (p=0,024) dengan KNF tipe WHO-1 dan 2. Uji korelasi antara ekspresi EBER dengan tipe histopatologi KNF positif kuat (koefisien korelasi Lambda = 0,667, p=0,034). Kesimpulan Ekspresi EBER terdapat lebih banyak pada KNF WHO tipe 3 yang berarti EBV lebih sering ditemukan pada KNF WHO tipe 3. Kata kunci: EBER, hibridisasi in situ, karsinoma nasofaring, virus Epstein-Barr. ABSTRACT Background The factors contributing nasopharingeal carcinoma (NPC) has been proven to be the interaction of viral, environmental and genetic factors. Etiopathogenesis of NPC is dominated by Epstein-Barr virus (EBV) infection, especially for WHO type 3. In Indonesia, there has been no publication about the expression of Epstein-Barr-virus-encoded small nuclear RNA (EBER) associated with histopathological classification. The aim of this study is to analyze the expression of EBER in various histopathological types of NPC and provide information onetiological diagnosis ofNPC that can be useful for therapeutic strategies. Methods This study was an descriptive designed as cross sectional study. Nasopharyngeal biopsies that were diagnosed as nasopharyngeal carcinoma, between the year 2009 and 2012, in Dr. Kariadi Hospital and St. Elisabeth Hospital Semarang were reevaluated. In situ hybridization with fluorescein-conjugated peptide nucleic acid (PNA) probe was performed on paraffin embedded tissue sections. Results This study found that 29 of 31 (93.5%) WHO-3 type NPC cases were EBER positif that significantly differed from WHO-1 and WHO-2 type NPC (p=0.024). There were strong positive correlation between histopathological types of NPC and expression of EBER (Lambda coefficient correlation=0.667, p=0.034). Conclusion EBER expressions found more in NPC WHO type 3 which mean EBV is often found in NPC WHO type 3. Key words: EBER, Epstein-Barr- virus, in situ hybridization, nasopharyngeal carcinoma.
Co-Authors Aris Haryanto Awal Prasetyo Dyah Widiastuti Hertanto, Nayaka Bagus Wahyu Agung Ina Kusrini Jajah Fachiroh, Jajah Kenty Wantri Anita, Kenty Wantri Nastiti Wijayanti Putranto, Bambang Endro Ramadhani, Fitriya Retno Murwanti