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All Journal Reaktor Biomedika VISI PUSTAKA: Buletin Jaringan Informasi Antar Perpustakaan Nusantara Science and Technology Proceedings JURPIKAT (Jurnal Pengabdian Kepada Masyarakat)
S. Marsudi
Fakultas Bisnis Dan Managemen Teknologi, Institut Teknologi Sepuluh Nopember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Energy Engineering Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Journal : Reaktor

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RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESS S. Marsudi
Reaktor Volume 10, Nomor 2, Desember 2006
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.849 KB) | DOI: 10.14710/reaktor.10.2.59-62

Abstract

Polyhydroxyalkanoates (PHAs) are intracellular material accumulated by several bacteria. Commercial production of PHAs faces the issue of high production cost especially substrate cost and recovery/separation cost. An alternative to reduce the production cost is to use enzyme and or chemical to recover PHAs from bacterial cells. Recovery of PHAs from bacterial cells was done using enzyme, chemical, and a mixture of enzyme and chemical. Enzyme (s) and or chemical(s) were added into culture broth to disrupt cells after adjusting pH and temperature of the culture broth. Treatment by adding enzyme or chemical only into culture broth showed a low level of PHAs recovered from bacterial cells. Treatment by adding a mixture of enzymes and chemicals showed the best result among 22 examined combinations, i.e. a mixture of EDTA, lisozyme, papain enzyme, and SDS. This combination gave a PHA recovery of 65 % w/w.
Co-Authors Ardy Maulidy Navastara Dini Ermavitalini Gita Widi Bhawika Irawati, Febri Dwi Kartika Nuswantara Kristanti Indah Purwani M. Syamsuddin Wisnubroto Moh. Saifulloh Nor Sri Inayati Nurul Jadid Soedarso Suarmini, Ni Wayan Tutik Nurhidayati