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All Journal Jurnal Ilmu dan Kesehatan Hewan Jurnal sains dan teknologi industri peternakan
I Made Yoga Windu Pradana
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Agriculture, Biological Sciences & Forestry Environmental Science Health Professions Social Sciences Veterinary

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The Effect of Soyabean Extender on Viability and DNA Integrity of Kintamani Dog Sperm on Cold Storage I Made Yoga Windu Pradana; Wayan Bebas; I Ketut Puja
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.103 KB)

Abstract

The objective of this study was to evaluate the viability and integrity of canine semen diluted in egg yolk glucose-citrate and Tris-citrate-glucose-soya bean. Semen was collected by manual manipulation from an apparently healthy kintamani dog stud. The semen was subjected to gross and microscopic examination to determine its viability following dilution in different extenders. Only semen with motility rate of 60% or higher was used in this study. Semen samples were diluted in Tris-citrate-glucose-soya bean and egg yolk glucose-citrate extenders at two levels of dilution; sperm to extenders ratio of 1:2 and 1:3. Following semen dilution, evaluations were made on sperm motility, percentage of live sperm, and DNA integrity at 0 hour, 3 hours, and 6 hours under 5oC of storage. Data was analyzed by multivariate analysis of variance (MANOVA).The present results showed a high significant different (p<0.01) in sperm motility, percentage of live and DNA integrity between treatment with egg yolk glucose-citrate and Tris-citrate-glucose-soya bean.  The result also showed that percentage of live sperm and DNA integrity of semen at 1:2 dilution difference significantly (p<0.01) to that of 1:3 dilution. The sperm viability and integrity were significantly difference (p<0,01) between the duration of storage at 5°C. Thus, the current study indicated that Tris-citrate-glucose- soya bean extenders  was sufficient to maintain motility, viability, and DNA integrity of kintamani dog  spermatozoa during storage at 5 oC. Further research should be conducted to evaluate the fertility of spermatozoa following dilution with these extenders.
PENANGANAN PROLAPS VAGINA PADA SAPI BALI DENGAN POLA ITERRUPTED HORIZONTAL MATTRESS Pradana, I Made Yoga Windu; Purnama, Dewa Ketut Raeyadi; Murdayasa, I Wayan Gede; Handika, Dewa Gede Crisna Ari; Guna, I Putu Sandika Arta
Jurnal Sains dan Teknologi Industri Peternakan Vol 6 No 1 (2026): Februari 2026
Publisher : Program Studi Peternakan Universitas Muhammadiyah Sidenreng Rappang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55678/jstip.v6i1.1935

Abstract

Vaginal prolapse is the protrusion of the vaginal mucosa from its anatomical position. Prompt and appropriate management is essential to prevent tissue necrosis. This paper discusses a case of vaginal prolapse in a Bali cow found in Kesiman Village, East Denpasar District, Bali. The affected cow was pregnant, with the vaginal mucosa observed hanging over the vulva. Treatment involved placing the cow in a restraint cage, cleaning the exposed vaginal mucosa with running water, and repositioning it to its normal position. This was followed by suturing the vulvar labia using an interrupted horizontal mattress pattern with nylon sutures to prevent recurrence. The sutures were removed 10 days post-treatment, and no signs of recurrent vaginal prolapse were observed.
Co-Authors Guna, I Putu Sandika Arta Handika, Dewa Gede Crisna Ari I Ketut Puja I Ketut Suatha I Putu Sampurna I Wayan Bebas Murdayasa, I Wayan Gede Purnama, Dewa Ketut Raeyadi