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All Journal International Journal of Renewable Energy Development BIOTROPIA - The Southeast Asian Journal of Tropical Biology ANNALES BOGORIENSES Jurnal Penelitian Kelapa Sawit Teknologi Indonesia Makara Journal of Technology Annales Bogorienses
Bambang Prasetya
Research Center for Biotechnology, LIPI, Cibinong, Bogor 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Veterinary

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Journal : ANNALES BOGORIENSES

 1 
Screening for Natural Producers Capable of Producing 1,3-Propanediol from Glycerol Andriani, Dian; Kusharyoto, Wien; Prasetya, Bambang; Wilke, Thomas; Vorlop, Klaus Dieter
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/43

Abstract

Glycerol is a renewable resource found as the main  by-product in the transesterification of triglycerides and fat saponification. Due to the increased production of plant oils, especially palm  oil  in developing countries, and their larger use  by the oleochemical industry, glycerol surpluses are on the world market and this may result in a desrease in glycerol  price. As a consequence, biotechnological processes  have been developed to convert this substrate  into  value-added  products,  such  as  1,3-propanediol  (1,3-PD).  The  microbial  production  of  1,3-PD could  be  competitive  to  chemical  routes  assuming  that  it  is  based  on  cheap  raw  material  and  an  optimized process.  In  the  screening  for  1,3  PD–producing  bacteria,  raw  glycerol  as  by-product  from  rapeseed  oil processing unit  was  used  as  a  carbon  source  compared  with  commercial  glycerol.  By  using  increasing concentration of  both  glycerols  from  50  to  150  g/l,  two  potential  bacteria  were  obtained  from  soil  samples.BMP 1 was obtained from an enrichment culture using 50 g/l commercial glycerol, while BMR-1 was obtained from  an enrichment culture using 100 g/l raw glycerol. The highest conversion yield obtained using the isolateBMP-1 was around 0.62 g 1,3-PD formed per  mol glycerol consumed, and 0.73  mol 1,3-PD  formed per  molgycerol using the isolate BMR-1. No bacteria were obtained from cultures using 150 g/l commercial and rawgycerol, respectively, which indicated that higher concentration of glycerol has inhibition effect.   Keywords: 1,3-propanediol, enrichment culture, glycerol, palm oil, screening
Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation Ratnakomala, Shanti; Yopi, Yopi; Suhartono, Maggy T; Meryandini, Anja; Prasetya, Bambang
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (685.031 KB) | DOI: 10.1234/96

Abstract

The objective of this study was to investigate the effect of media compositions on the production of β-mannanase by Nonomuraea sp.ID06-379. The study was focused on the influence of carbon, nitrogen,phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing β-mannanase (1.075 ± 0.006 U/ml),(NH4)2HPO4 as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH4)2HPO4 were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of β-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH4)2HPO4, under submerged fermentation with shaking at 120 rpm and 28 C for 2 days incubation.
Application of Microwave Heating in Biomass Hydrolysis and Pretreatment for Ethanol Production Hermiati, Euis; Mangunwidjaja, Djumali; Sunarti, Ttiti C; Suparno, Ono; Prasetya, Bambang
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.18 KB) | DOI: 10.1234/33

Abstract

Recently, due to depletion of  fossil derived energy stock in the world, there are growing  interests in utilizing biomass sources of bioethanol. There are basically two types of biomass that are usually used or converted to ethanol, starchy and lignocellulosic biomass. The conversion of starchy and lignocellulosic materials are widely explored,  however,  there  are  still  some  drawbacks,  such  as  high  enzyme  cost  and  intensive  energy  needed. Therefore, lower cost as well as energy and time efficient process technology in biomass conversion to ethanol  is important in enhancing the use of biomass to substitute fossil fuel. Microwave heating offers some advantages to overcome these drawbacks, especially due to its quick heat transfer and its heating selectivity. In conventional heating the  heat  was  transferred  through  conduction  or  convection  process  which  took  longer  time.  Thus,  by using microwave, degradation of starchy and lignocellulosic biomass could be completed in shorter time than by using conventional heating method. The roles of microwave heating in the degradation of biomass, especially starchy and lignocellulosic biomass  and its relation to the hydrolysis and pretreatment of that particular biomass for ethanol production are reviewed and discussed.   Keywords: biomass, pretreatment, ethanol, microwave
Co-Authors Aliyah, Novin Anas Miftah Fauzi Anja Meryandini Anondho Wijanarko Budi Mulyono, Ary Changshin Sunwoo, Changshin Dian Andriani, Dian Djumali Mangunwidjaja Don-Hee Park, Don-Hee Dulbert Tampubolon, Biatna Dwi Susmiarni, Rika Edita Martini, Edita Ellia Kristiningrum Euis HERMIATI Febrian Isharyadi Heri Hermansyah I Nyoman Jaya Wistara Kang, Kyeong Eop Khaswar Syamsu Kusharyoto, Wien Kusharyoto, Wien Kyeong Kang, Kyeong Lanjar Sumarno Lucky Risanto M Baiquni M Samsuri, M M. Baiquni Maggy T Suhartono Maggy Thenawidjaja Suhartono Mardias, R. Misri Gozan Mohammad Nasikin Nasikin, M. Nastiti Siswi Indrasti Ono Suparno R Mardias, R Restu Wahono, Daryono S Heris Anita, S Heris Samsuri, M. SHANTI RATNAKOMALA Sriramulu GobiKrishnan, Sriramulu Sunarti, Ttiti C Surn-Teh Bark, Surn-Teh Titi C. Sunarti Tjahyo Eka Darmayanti, Nur Utari Ayuningtyas Vorlop, Klaus Dieter Vorlop, Klaus Dieter Wasrin Syafii Widya FATRIASARI Wilke, Thomas Willke, Thomas Wulansari, Nuri YOPI YOPI