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Hubungan Dosis Tepung Gembili (Dioscorea esculenta) Dengan Tingkat Ekspresi Enzim Ampk-α2 pada Model Tikus Diabetes Melitus neni oktiyani; sunarti Sunarti; Prasetyastuti Prasetyastuti; Jujuk Anton Cahyono
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (995.684 KB) | DOI: 10.31964/mltj.v1i1.4

Abstract

Alternative methods of controlling glucose levels in patients with diabetes is by type of food, either by utilizing yam flour. At the flour contained inulin and resistant starch that can activate the enzyme AMPK-α2. Activation of these enzymes will stimulate glucose transport in skeletal muscle and liver, thus causing a decrease in glucose production. Varying doses of flour is expected to affect the expression of AMPK-α2. This study aims to dosage relationship yam flour (Dioscorea esculenta) with tigkat-α2 AMPK enzyme expression in the nucleus skeletal muscle and liver in mouse models of diabetes mellitus. The study was a quasi-experimental design with Post Test Only Group Design. Rats were divided into 5 (five) groups, healthy mice, the mice with type 2 diabetes, and type 2 groups of diabetic rats with dietary intake yam flour addition of 1.25 g (TG-1:25),), 2.5 g (TG -2.5), 5.0 g (TG-5.0). Yam flour is mixed into the rat diet feed with varying doses. The results showed no significant correlation between the dose of yam flour with AMPK-α2 expression levels in skeletal muscle nuclei (p = 0.312) and liver (p = 0.474) in a mouse model of DM. The need for other studies using other types of food as an alternative arrangement of food for patients with diabetes
Pengaruh Polymorphisme PvuII Gen Reseptor Estrogen Alfa terhadap Kadar Kalsium dan Phosphat Serum pada Wanita Postmenopause Prasetyastuti Prasetyastuti
Berita Kedokteran Masyarakat (BKM) Vol 26, No 1 (2010)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.478 KB) | DOI: 10.22146/bkm.3479

Abstract

Background: Estrogen has an important role in bone metabolism. The hypoestrogenemia condition occurring in postmenopausal women may affect bone metabolism. The mechanism action of estrogen to prevent the activity of osteoclast in bone resorption was mediated especially by estrogen receptor alpha gene (ESR1). The PvuII polymorphism that occurs in ESR1 gene may alter their expression and  function.Objectives: the aim of this research was to determine the difference of serum calcium and phosphate level in postmenopausal women with PvuII polymorphism of the Estrogen receptor alpha geneMethod: This was cross sectional design. The subjects are postmenopausal women 50-70 year old .Twenty seven subject divided into two group, group I consisted of 15 women with genotype PP,Pp and group II consisted of 12 with genotype pp. polymorphism were assessed by Polymerase Chain Reaction Restriction Fragmen Length Polymorphism(PCR-RFLP) technique. Determination of serum calcium and phosphate level used spectrophotometer. The data was analyzed with Independent sample t-test.Results: The serum calcium level are 8,15 ± 1,35 mg/dl and 8,13 ± 1,58 mg/dl in genotype PP,Pp and pp respectively. The serum phosphate level are 3,8 ± 0,47 mg/dl and 4,24 ± 0,86 mg/dl in genotype PP,Pp and pp respectivelyConclusion: there were no statistically significant differences in serum calcium and phosphate level among genotype PP,Pp with pp  ESRI gene(p>0,05) PvuII polymorphism of ESRI gene did not influence serum calcium and phosphate level in postmenopausal women.Keywords: postmenopausal women, estrogen receptor polymorphism, serum calcium, serum phosphate
Korelasi Antara Kadar Vitamin C dengan Kadar Methemoglobin Darah Lansia di Kecamatan Pakem Kabupaten Sleman Prasetyastuti Prasetyastuti
Berita Kedokteran Masyarakat (BKM) Vol 25, No 1 (2009)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.427 KB) | DOI: 10.22146/bkm.3572

Abstract

Background: Oxidative damage  by  free  radical can  cause  aging  process. Erythrocyte  as an  oxygen –  carrying agent  has high  risk  of  oxidative  injury which can  inducemethemoglobin  production.  Vitamin C  is  an  exogen antioxidant  that work  in  aqueous  phase  like  blood  and  can  react   with    free  radical before  they  initiate  the oxidative  injury.Objectives: The  aim of  this study was  to  find out whether  there  is possibility  of correlation  between  vitamin C and methemoglobin  level  in blood of elderly  living  in Pakem DistrictMethod:  This was  an observational  research with  cross  sectional  design,  in which  32  elderly  from  Pakem District were  subjects.  The  blood  sample was  taken  from  cubital  vein.  Determination  of   vitamin C  and methemoglobin  level  used  spectrophotometer. Pearson Correlation was  employed  to  analyze  the  data.Results: The vitamin C and methemoglobin level of the elderly 0,43 + 0,25 mg/dl  and  0,035 +  0.02% respectively. The  correlation between  vitamin C  and methemoglobin  level was  not significant    (  p =  0.771).Conclusion: The  vitamin C was not  correlated with  the methemoglobin  level.Keywords: vitamin C, methemoglobin,  elderly,  free  radica
Fibrotic effect of monoculture, conditioned media and coculture of fibroblast NIH3T3 cells on collagen secretion and mRNA TGF-beta expressions Rizki Awaluddin; Dwi Aris Agung Nugrahaningsih; Prasetyastuti Prasetyastuti
Pharmaciana Vol 13, No 2 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i2.25482

Abstract

Fibrosis is common profound in diabetes condition, they characterized by accumulation of extracellular matrix in tissue then cause tissue injury. The study aim to investigate effect of monoculture, conditioned media, and coculture of NIH3T3 cells in LPS-high glucose media conditions on mRNA TGF-B and collagen secretion. The fibroblast NIH3T3 were cultured with various culture type there is mono culture NIH3T3, conditioned media from RAW264.7 culture media, and coculture with RAW264.7 cells (ratio 1:1 and 1:2)  in normal media (media containing 4500 mikro g/mL of glucose) and HG-LPS media (media containing LPS 0.25 mikro g/mL and D-glucose 5400 mikro g/mL). Our study shows that mRNA TGF-B and collagen secretion of fibroblast NIH3T3 cells in monoculture and conditioned media from inflammatory macrophages are not statistically different. On other hand, the TGF-B mRNA expression and the relative ratio of collagen secretion in RAW 264.7 and NIH 3T3 cell coculture media with a culture ratio of 1:1 were the highest compared to other models (p kurang dari 0.05). Our study conclude that RAW 264.7 and NIH 3T3 coculture cells (1:1) in LPS and high glucose media is appropriate for fibrosis in vitro models.
Pengenalan Nutrigenomik Pada Guru SMA Biologi dan Kimia di Kota Yogyakarta Melalui Program Kemitraan Masyarakat (PKM) Farmawati, Arta; Sunarti, Sunarti; Rubi, Dianandha Septiana; Sadewa, Ahmad Hamim; Prasetyastuti, Prasetyastuti; Ngadikun, Ngadikun
SWARNA: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 2 (2024): SWARNA: Jurnal Pengabdian Kepada Masyarakat, Februari 2024
Publisher : LPPM Sekolah Tinggi Ilmu Ekonomi 45 Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/swarna.v3i2.1177

Abstract

Perkembangan ilmu pengetahuan dan teknologi berdampak pada pengembangan ilmu nutrisi berbasis molekuler diantaranya adalah nutrigenomik. Nutrigenomik merupakan ilmu yang mempelajari bagaimana nutrisi mempengaruhi ekspresi gen. Gen membawa deoxyribose nucleic acid (DNA) yang berisi informasi genetik yang menentukan sintesis protein, dimana protein tersebut berperan dalam berbagai proses biologis, sehingga sangat mempengaruhi kesehatan. Proses biologis merupakan proses reaksi kimia dalam tubuh yang sudah dipelajari sejak sekolah menengah pertama (SMP) oleh karena itu, pengenalan ilmu nutrigenomik pada guru SMA diharapkan dapat meningkatkan pengetahuan terhadap ilmu nutrigenomik pada guru, sehingga dapat meningkatkan kualitas siswa maupun guru sendiri. Upaya ini kami wujudkan dalam bentuk pengabdian masyarakat yang bekerja sama dengan Musyawarah Guru Mata Pelajaran (MGMP) Biologi dan Kimia Kota Yogyakarta. Kegiatan pelatihan yang diikuti 18 guru dilaksanakan dengan beberapa metode pembelajaran seperti ceramah, praktik di laboratorium, tutorial, dan training of trainer (ToT). Berdasarkan hasil evaluasi, program kemitraan masyarakat (PKM) dapat meningkatkan pengetahuan guru-guru mengenai nutrigenomik secara signifikan (43,33 ± 10,01 vs 66,44 ± 13,66) (P<0,05). Hasil ToT menunjukkan tingkat kepercayaan diri guru-guru dalam menyampaikan informasi terkait dengan nutrigenomik menjadi tinggi. Dengan demikian, program ini dapat mengenalkan sekaligus meningkatkan pengetahuan dan rasa percaya diri guru sebagai kader nutrigenomik untuk menyebarluaskan ilmu nutrigenomik kepada siswa maupun masyarakat.
Wild Stinging Nettle (Urtica dioica) Extract Suppresses Visceral Adipose Aromatase Levels and Improves Lipid Profile in Male Obese Rats Zaenudin, Zaenudin; Tyas, Jurnalis Gempaning; Tangkari, Kabir Ardiansyah; Sutiani, Harni; Farmawati, Arta; Prasetyastuti, Prasetyastuti
Journal of Food and Culinary Vol. 8 No. 1 [Juni 2025]
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jfc.v8i1.13860

Abstract

Obesity can elevate estrogen levels through increased aromatase activity, adversely affecting male fertility. Although aromatase inhibitors are commonly used, they can disrupt lipid profiles and raise cardiovascular risks. This experimental study analyzed the effects of wild stinging nettle (Urtica dioica) extract on visceral adipose aromatase levels and serum lipid profiles in obese male rats. Twenty-five 7–8-week-old male Sprague Dawley rats were divided into five groups: normal control, obesity control, and three obesity groups receiving U. dioica extract at 125, 250, or 500 mg/kg body weight. After a 4-week intervention, blood samples were collected to measure lipid profiles, and visceral adipose tissue was harvested to assess aromatase levels. The U. dioica extract significantly reduced visceral adipose aromatase levels (p < 0.01) and improved lipid profiles in obese rats. Specifically, treated rats showed dose-dependent decreases in triglycerides, total cholesterol, and LDL-cholesterol, along with an increase in HDL-cholesterol (p < 0.05). These findings indicate that U. dioica extract can suppress adipose aromatase levels and ameliorate lipid disturbances in obesity.