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Hernawan Yuli Rahmadi

Indonesian Oil Palm Research Institute

Author-ID : 3464638

Agriculture, Biological Sciences & Forestry

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KERAGAMAN GENETIK POPULASI E. oleifera DAN POPULASI E. guineensis x E. oleifera PADA KOLEKSI PLASMA NUTFAH PPKS Rokhana Faizah; Sri Wening; Hernawan Yuli Rahmadi; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 24 No 1 (2016): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

The oil palm species of Elaeis oleifera have some superior characters not possessed by E. guineensis species. The cross between E. oleifera x E. guineensis (E. o x E. g.) Is carried out to insert the superior character of E. oleifera into the E. guineensis species and to obtain individuals who possess a superior characteristic blend of both species. This study aims to determine the genetic diversity within and between populations of E. oleifera, as well as relationships between E. oleifera, E. guineensis and its interspecific hybrids. A total of 8 germplasm populations used were E. oleifera originating from Suriname and Brazil, E. guineensis population of Dura Deli and SP540T, and interspecific crosses of hybrid E. guineensis x E. oleifera from Colombia, hybrid E. guineensis x E. oleifera which is presumably from Colombia, as well as hybrid E. guineensis x E. oleifera from Brazil. A total of sixteen Simple Sequence Repeat (SSR) markers were used to analyze 92 individuals from 8 populations. PCoA results show that 8 Elaeis populations cluster in each group. The hybrid population of E. guineensis x E. oleifera suspected of Colombia has a very close kinship relationship with hybrid E. guineensis x E. oleifera from Colombia and clumped in the same quadrant. This suggests that the hybrid is indeed a hybrid of E. guineensis x E. oleifera from Colombia. The population of E. oleifera from Suriname showed the lowest genetic diversity, with the number of different alleles, specific alleles, heterozygosity values, and the percentages of polymorphic loci sequentially were 1.37, 0.18, 0.09, and 37.50%. While the population of interspecific interspecific crossbreeds of the E. oleifera species showed the highest genetic diversity with values of 3.81, 0.43, 0.62 and 100%.

DUGAAN GEJALA DEPRESI SILANG-DALAM DAN TINGKAT HOMOZIGOSITAS POPULASI KELAPA SAWIT HASIL PENYERBUKAN SENDIRI GENERASI KE-4 SP540T DAN GENERASI KE-5 DURA DELI Rokhana Faizah; Sri Wening; Hernawan Yuli Rahmadi; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 24 No 2 (2016): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Inbreeding is a common method used to reproduce candidate mother plant from selected parental lines for commercial seeds in Reciprocal Recurrent Selection (RRS) oil palm breeding program. However this practice may increased homozigosity level of selected population. This study concerned the level of homozygosity of SP540T fourth generations and Dura Deli Dolok Sinumbah fifth generations (3 crosses respectively) and their correlation with inbreeding depression symptoms. Polymerase Chain Reaction-Simple Sequence Repeat (PCR-SSR) with 16 markers developed for oil palm was used to analyze 327 samples. The result shows that the levels of homozigosity of SP540T fourth selfing generation were ranged between 0.44-0.84 or 0.61 in average. While the levels of homozygosity of Dura Deli fifth selfing generations were ranged between 0.60-0.93 or 0.78 in average. The homozygosity level in Dura Deli was 1.27% higher than SP540T populations. Correlation analysis showed that the higher the level of homozygosity, the higher of the inbreeding symptoms 2 observed (R =0.95).

KERAGAAN VEGETATIF DAN KOMPONEN TANDAN DARI SILANG BALIK SEMU PERTAMA Elaeis oleifera ORIGIN BRAZIL DAN SURINAME DI SUMATRA UTARA, INDONESIA Heri Adriwan Siregar; Hernawan Yuli Rahmadi; Retno Diah Setiowati; Edy Suprianto
Jurnal Penelitian Kelapa Sawit Vol 26 No 1 (2018): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

An Attempt to combine the superior traits of Elaeis oleifera and Elaeis guineensis have been done through an interspecific hybrid cross and followed by pseudo-backcross 1 (pBC1). Observation of vegetative morphology and bunch components are presented in this paper. Two populations of pBC1 E. oleifera from the Suriname and Brazil origin were planted in 1990, 1993, 1995, and 2005, and were intensively observed for vegetative morphological properties and bunch components in November 2016 to February 2018. The results showed that almost all the individuals of pBC1 grew upright such as E. guineensis, no longer growing horizontally like the wild E. oleifera and the interspecific hybrid populations. The datas showed that the Suriname population plant architecture are compact or smaller than the Brazilian origin including the height increment and the size of the stem, the frond architecture and its components. Similarly, the bunch components show that the pBC1 Brazil is slightly superior to Suriname pBC1.

PERAN NAA, GA3, KARBON AKTIF, DAN SUKROSA DALAM KULTUR EMBRIO ZIGOTIK KLON OG HYBRID (Elaeis guineensis JACQ. X Elaeis oleifera) OPEN POLLINATED Ernayunita Ernayunita; Hernawan Yuli Rahmadi; Iman Yani Harahap; Abdul Razak Purba
Jurnal Penelitian Kelapa Sawit Vol 24 No 3 (2016): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Oil palm OG hybrid genetic potentials are high in term of vegetative and generative traits, such as: low height increment, compact palm, high free fatty acid and high carotenes. However, OG hybrid reproduction is hindered due to its low germination rate. So, alternative method for germination is needed, which is in vitro embryo zygotic culture. This study objective was to reveal the best medium composition for OG hybrid embryo culture by modificated several oil palm basic medium from oil palm tissue culture protocol and to understand the role of plant growth regulators, activated charcoal, and sucrose on in vitro growth and development of OG hybrid embryo. The design used was Completely Randomized Design (CRD) with 16 replication and 4 embryos for each replicates, while the treatments were: A1 = M129 (control); A2 = M129 + activated charcoal; A3 = M129+ activated charcoal + sucrose; A4 = ½ M129 + activated charcoal + sucrose; A5 = M129 + GA3 ; and A6 = M129 + GA3 + NAA. From the study we found that the best medium for OG hybrid open pollinated clone's embryo culture was M129 with GA3 and NAA (A6). This medium gave percentage of in vitro germination up to 14.06%, and later on the plantlets from this medium gave the best vegetative growth performance such as high number of leaves and primer roots.

Co-Authors Abdul Razak Purba Edy Suprianto Ernayunita Ernayunita Heri Adriwan Siregar Iman Yani Harahap Retno Diah Setiowati Rokhana Faizah Sri Wening

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