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Journal : Journal of Tropical Animal Science and Technology

Levels Protein and Fat of Yoghurt Made of Different Types and Number of Cultures Theresia Ika Purwantiningsih; Maria Adlofina B Bria; Kristoforus W Kia
Journal of Tropical Animal Science and Technology Vol 4 No 1 (2022): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v4i1.967

Abstract

This research was carried out in June 2020 at the Chemistry Laboratory of the Faculty of Animal Husbandry, Nusa Cendana University, Kupang for testing protein and fat levels. This study aims are to determine the protein and fat content of yoghurt made from various types of milk, the protein and fat content of yoghurt made from different amount cultures, and to determine the interaction between the type of milk and the number of yoghurt cultures. Research material in the form of fresh milk, UHT milk, yoghurt cultures. The design used in this study was a completely randomized design (CRD) with a factorial pattern using 2 treatment factors, namely factor A in the form of type of milk, which consisted of: A1 = fresh milk, A2 = UHT milk (Ultra High Temperature) and factor B in the form of culture. yoghurt consisting of: B1 = Lactobacillus bulgaricus and Streptococcus thermophilus, B2 = Lactobacillus acidophilus, Lactobacillus bulgaricus, and Streptococcus thermophilus, B3 = Streptococcus thermophilus, Lactobacillus adhophilus, Lactobacillus bulgaricus, and Bifidobacterium animals. From the two factors above, there were 6 treatment combinations consisting of: A1B1, A1B2, A1B3, A2B1, A2B2, A2B3, each combination consisting of 3 replications so that there were 18 experimental sample units. The results showed that the type of milk and the number of bacterial cultures affected the fat content and protein content of yoghurt and there was an interaction between the type of milk and the number of different cultures on the percentage of fat and protein content of yoghurt produced. types of milk with different culture brands had a significant effect (P<0.05) on the percentage of yoghurt fat content. The results of the follow-up test with Duncan's test showed that the combination of treatment between the type of UHT milk and the culture Lactobacillus bulgaricus had a significant effect (P<0.05) on the percentage of yoghurt protein content in all treatments. It can be concluded that fresh milk has higher protein and fat content than UHT milk. The number of cultures 2 (Lactobacillus bulgaricus and Streptococcus thermophilus) produced yoghurt with the highest fat content and protein content.
The Effect of Filteret Sugarcane Juice in Egg Yolk Citrate on Viability and Spermatozoa Abnormality, and pH of Bali Catle Semen Rindiyani Y Tanii; Agustinus A Dethan; Theresia Ika Purwantiningsih
Journal of Tropical Animal Science and Technology Vol 4 No 1 (2022): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v4i1.1098

Abstract

This study aims to determine the effect of the sugarcane juice diluent in egg yolk citrate on viability, spermatozoa abnormality, and pH of Bali bull semen. This study has been carried out in Poultry Housing of Animal Science Study Program, and the Laboratory of the Faculty of Agriculture, University of Timor. Semen is collected from a a bull, ± 4.5 years old, in healthy condition. The method used in this study was a completely randomized design method with 5 treatments and 4 replications. The treatment giving are: T0 (diluent citrate-egg yolk without sugarcane juice); T1 (diluent citrate-egg yolk + 5ml sugarcane juice); T2 (diluent citrate-egg yolk + 10 ml sugarcane juice); T3 (diluent citrate-egg yolk + 15 ml sugarcane juice) and T4 (diluent citrate-yolk + sugar cane juice 20 ml). The variables measured were viability, abnormal spermatozoa, and semen pH of Bali bull. The results showed that T4 treatment with a 20 ml sugarcane juice level showed the best value for spermatozoa viability (92.875%), spermatozoa abnormality (6.375%), and semen pH (6.6). Conclusion: that the level of 20 ml of sugarcane juice in semen diluent can maintain the life of Bali bull spermatozoa.
Effect of Feeding Several Energy Sources on Carcass Production of Local Male Pigs in the Grower Phase Seran, Alfiana; Klau Tahuk, Paulus; Purwantiningsih, Theresia Ika
Journal of Tropical Animal Science and Technology Vol. 7 No. 2 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v7i2.690

Abstract

The study aimed to determine the carcass characteristics of local male pigs fed different energy sources. The research location is in Sasi Village, Kefamenanu District, North Central Timor Regency. The livestock used are 12 castrated local male pigs aged 12–16 weeks with a body weight range of 5–7 kg. The method used in the study is an experimental method using a completely randomised design (CRD) with 3 treatments and 4 replicates. The treatments tested were as follows: T1: 40% ground corn + 23% rice bran + 15% bran pollard + 22% fish meal; T2: 15% ground corn + 40% rice bran + 23% bran pollard + 22% fish meal; T3: ground corn 23% + rice bran 15% + pollard bran 40% + fish meal 22%. The variables observed in this study were slaughter weight, carcass weight, non-carcass weight, carcass percentage, and non-carcass percentage. The results showed that the treatments had no significant effect (P > 0.05) on slaughter weight, carcass weight, non-carcass weight, and carcass and non-carcass percentages of local male pigs in the growing phase. The slaughter weight for each treatment (T1, T2, and T3) was as follows: T1 was 6.867 kg, T2 was 7.936 kg, and T3 was 8.498 kg; the carcass weight for each treatment (T1, T2, and T3) was as follows: T1 was 3.466 kg, T2 at 4.115 kg, and T3 at 4.621 kg; the non-carcass weight for treatment T1 was 3.401 kg, T2 at 3.821 kg, and T3 at 3.886 kg; the carcass percentage for treatment T1 was 50.094%, T2 was 51.766%, and T3 was 52.674%; while the non-carcass percentages for treatments T1, T2, and T3 were 49.905%, 48.234%, and 47.325%, respectively.  It can be concluded that the use of ground corn, rice bran, or bran pollard as an energy source feed results in carcass and non-carcass production of local male pigs in the growing phase that are not significantly different, although there is a higher tendency when using 40% bran pollard in the diet.
Isolation of the bacteria that cause mastitis in dairy cows on farms in Yogyakarta Purwantiningsih, Theresia Ika; Widyobroto, Budi; Sine, Yuni; Suranindyah, Yustina; Artama, Wayan Tunas; Widodo
Journal of Tropical Animal Science and Technology Vol. 7 No. 3 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v7i3.10231

Abstract

Mastitis merupakan penyakit yang disebabkan oleh bakteri dan pada umum menyerang ternak perah. Berdasarkan hasil penelitian sebelumnya, terdapat perbedaan jenis bakteri penyebab mastitis di setiap daerah. Mengidentifikasi bakteri penyebab mastitis perlu dilakukan agar penyakit ini dapat ditangani dengan cepat, tepat, efektif dan efisien. Tujuan penelitian ini adalah untuk mengidentifikasi bakteri yang berpotensi menyebabkan mastitis di salah satu peternakan sapi perah di Yogyakarta. Pengujian California Mastitis Test dilakukan sebelum pengambilan sampel susu. Sampel susu yang menunjukkan hasil positif tiga kemudian diambil untuk diisolasi bakteri yang ada di dalamnya. Sampel susu yang berasal dari sapi yang terkena mastitis di peternakan tersebut diinolulasikan ke dalam bood agar, isolat yang tumbuh kemudian diinokulasikan kembali untuk selanjutnya dilakukan beberapa pengujian, seperti pewarnaan Gram, uji katalase, uji motilitas dan identifikasi menggunakan PCR. Hasil penelitian menunjukkan bahwa dua isolat dominan yang dihasilkan menunjukkan ciri – ciri berwarna ungu, berbentuk bulat, hasil uji katalase negatif dan hasil uji motilitas juga negatif. Hasil identifikasi menggunakan PCR menunjukkan bahwa kemungkinan spesies bakteri yang berhasil diisolasi adalah Streptococcus agalactiae, Bacillus cereus ,Bacillus thuringiensis, Bacillus wiedmannii, dan Endophytic bacterium. Streptococcus agalactiae dan Bacillus cereus merupakan bakteri dominan yang berhasil diisolasi dan kedua bakteri tersebut berpotensi sebagai penyebab mastitis pada peternakan di daerah Yogyakarta.