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Tjokorda Gede Oka
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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SARI CenTeLLA ASIATICA ASLI BALI MENINGKATKAN SEKRESI TuMouR neCRoSIS FACToR ALPHA (TNF-a) PADA MENCIT YANG DIINFEKSIKAN SALMoneLLA TYPHI I Nyoman Wande; Sianny Herawati; Ida Ayu Alit Widhiartini; I Wayan Putu Sutirta Yasa; Tjokorda Gede Oka; Ni Made Linawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.477

Abstract

Tumour necrosis factor alpha (TNF-α) is a cytokine produced by macrophages and other mononuclear cells, is a good antibacterial agent against Salmonella spp, especially Salmonella typhi. Centella asiatica is an alternative drug that is expected as an immunostimulant in patients with typhoid fever. Comparing the effectiveness of Centella asiatica extract the original Bali as an immunostimulant and without stimulants in mice infected Salmonella typhi in terms of TNF-α secretion. This study is an experimental study with a post test only with control group design. A total of 20 mice were divided into 4 groups. The first and second groups each given Centella asiatica extract 75 mg/20 g bw (0.5 cc) and without a given extract for 4 weeks. Both groups were inoculated orally Salmonella typhi 106 per mL of bacteria in the second week. The third and fourth groups were given thiamphenicol with Centella asiatica extract 75 mg/20 g bw (0.5 cc) and thiamphenicol without any extract for 4 weeks respectively. Both groups were inoculated orally Salmonella typhi 106 per mL of bacteria in the first day. All groups terminated on fourth week and examination levels of TNF-α by ELISA and gall culture. The mean levels of TNF-α in groups (1–4) is 86.10±2.67 pg/mL, 32.81±11.33 pg/mL, 35.87±3.90 pg/mL and 19.21±2.19 pg/mL respectively. Based on the examination of the gall cultures this study showed positive results in the first and second groups, while a negative result on the third and fourth groups. Based on the One way ANOVA analysis on levels of TNF-α, there are significant differences between the first group with the second group (p<0.05), and between the third and fourth groups also found significant differences (p<0.05) increased levels of TNF-α in mice with Salmonella typhi infection given Centella asiatica extract.
SUHU PENYIMPANAN KREATININ DAN ASAM URAT DALAM AIR KEMIH SELAMA 24 JAM AAN. Subawa; Sianny Herawati; I Nyoman Wande; I Wayan Putu Sutirta Yasa; Tjokorda Gede Oka
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1107

Abstract

Creatinine and uric acid is a product that excreted in the urine by normal kidney functions. The examination of creatinine and uricacid in urine is done on 24-hour urine collection. During the storage of the urine, it is recommended to be stored in a refrigerator withthe grade temperatures ranging from 2–8°C and is not recommended to use any preservative for the examination of creatinine anduric acid in urine. To know the comparation of creatinine and uric acid concentrations in urine between the urine tested immediatelyafter the collection with urine that was stored at a temperature 2–8°C and those at room temperature for 24 hours. A total of 45 urinesamples from outpatient clinic that came to the laboratory, were collected in particular urine vacutainer. Each urine sample is divided intothree tubes. The first tube (P1) examined concentrations of creatinine and uric acid immediately after collection, was considered as thebaseline value. The second tube (P2) stored at 2–8°C and the third tube (P3) is stored at room temperature for 24 hours, then followedby the examination of creatinine and uric acid concentrations. The examination of creatinine in urine was using reagent CREP2 RocheDiagnostic and uric acid in urine was using reagent UA2 Roche diagnostics by Cobas Integra ® 400 plus ® instrument. The mean ofcreatinine in urine concentrations which immediately examined (P1) is (125.10±74.85 mg/dL), concentrations after storage at 2−8°C(P2) and at room temperature (P3) were (123.42±73.80 mg/dL) and (124.09±73.95 mg/dL) respectively. Based on the analysis ofone-way ANOVA, there were no significant differences between the concentrations of creatinine in urine immediately checked which werestored at 2–8°C and at room temperature (P>0.05). The mean of uric acid in urine concentrations which immediately examined (P1) is(52.61±35.48 mg/dL), where as after storage at 2–8°C (P2) and room temperature (P3) were (45.11±31.62 mg/dL) and (46.38±28.91mg/dL) respectively. Based on the analysis of one-way ANOVA, there were no significant differences between the concentrations of uricacid in urine immediately checked by those stored at 2–8°C and at room temperature (P>0.05). Based on this study, it can be concludedthat there were no effect of storage temperature on the concentrations of creatinine and uric acid in urine within 24 hours.
PENDERITA DENGAN HEMOKROMATOSIS PRIMER Kadek Mulyantari; A.A.Wiradewi Lestari; A.A.N. Subawa; Tjokorda Gede Oka; Sudewa Djelantik
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 2 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i2.1014

Abstract

Primary Hemochromatosis is a hereditary disease that occurs predominantly in man. Among men, clinical signs and symptomsfrequently appears on 40 years until more than 60 years of age. Meanwhile, the signs and symptoms among women appear on 50 yearsof age or after menopause. It is a very rare case in children or young adult. Secondary hemochromatosis can be differentiated fromprimary hemochromatosis based on existence of other underlying disease and secondary hemochromatosis often occurs in patient withmultiple blood transfusions. The diagnosis of primary hemochromatosis is confirmed by chromosomal test and liver biopsy to confirmthe liver damage caused by excessive iron accumulation. The main treatment of primary hemochromatosis is phlebotomy. The purposeof this method is to remove overload iron in body. In this case, the patient was man, unmarried, 51 years old, Australian. Four yearsago, he had complained about arthropathies, chronic asthenia, depression, decreased of concentration and sexual desire. Laboratoryevaluation revealed Ferritin level 2126 ug/L and transferrin saturation always more than 99%. Liver function tests also increasedsignificantly. Some of his family’s members have the same disease as he has. He was diagnosed as primary hemochromatosis and hadperformed phlebotomy routinely. After phlebotomy has done, he recovered based on clinical and laboratorial findings.
Co-Authors Anak Agung Ngurah Subawa Anak Agung Wiradewi Lestari I Nyoman Wande I Wayan Putu Sutirta Yasa I.A.A. Widhiartini Ni Kadek Mulyantari Ni Made Linawati Sianny Herawati Sudewa Djelantik