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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Hardjoeno Hardjoeno
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HASIL TES LAJU ENDAP DARAH CARA MANUAL DAN AUTOMATIK N. Ibrahim; Suci Aprianti; M. Arif; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.840

Abstract

Erythrocyte sedimentation rate (ESR) is a non specific test that describes changes in protein plasma and is useful to determine inflammatory case. ESR is determined by using manual and automatic. In big cities, automatic method is more popular now days, but in periphery area, manual method is still the choice. To find out correlation between the results of manual and automatic tests. Cross sectional study was carried out among 200 patients at dr. Wahidin Sudirohusodo Public Hospital of Makassar from September to October 2005. The data were analyzed using R-Square (R2) and regression test using SPSS 11.5. There is significant correlation (P<0.05) among vertical manual, oblique manual and automatic methods. R2 of automatic and vertical methods are 0.95 (the first one hour) and 0.94 (the second one hour), as well as the R2 of oblique and vertical methods are 0.62 (the first one hour) and 0.87 (the second one hour) respectively. There is correlation among the results of the three methods. To convert the result of automatic to vertical, an equation of vertical score = 4.32 + 1.00 (automatic score) can be used. Meanwhile, conversion of oblique score to vertical score is vertical score = 7.58 + 0.99 (oblique score).
ANALISIS CAIRAN DARAH (TRANSUDAT) DAN SERUM CAMPURAN (EKSUDAT) DI PENDERITA DENGAN REMBESAN SELAPUT PARU (EFUSI PLEURA) Didi Irwadi; Sulina Y. Wibawa; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 2 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i2.947

Abstract

Pleural effusion is a fluid excess into pleural cavity due to transudation or exudation processes. The fluid deposited in the cavity canthreat the patient’s life. The pleural effusion could be produced in a patient with tuberculosis, cancer, cardiac failure, renal failure orviral/bacterial infection. The study is aimed to analyze the patterns of substance in the pleural effusion fluids produced by differentdiseases. A cross sectional study was performed from June 2006 to June 2007 at Clinical Pathology Laboratory of Dr. WahidinSudirohusodo Hospital, Makassar. The fluids were tested for glucose, total protein, LDH, and leukocyte count. Of 87 pleural effusion fluidsamples from 14–80 years old patients, 34.5% were transudates and 65.5% were exudates. Glucose value was higher (one tail T test,p < 0.01) in transudates group, whereas protein, LDH and leukocyte count were higher (one tail T test, p < 0.01) in exudates group.There were no significant differences of glucose, protein, LDH and leukocyte count among diseases within transudates group, as well aswithin exudates group. Staphylococcus spp., Klebsiella spp., and Acinetobacter spp., were the predominant bacteria revealed from thefluid cultures. Values of glucose, protein, LDH and leukocyte count have a different pattern between transudates and exudates groups.However, no special patterns were found among diseases within groups.
ANALISIS KADAR OSTEOKALSIN SERUM OSTEOPENIA DAN OSTEOPOROSIS N Sennang AN; Mutmainnah Mutmainnah; RDN Pakasi; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.841

Abstract

Osteoporosis is a metabolic bone disease, characterized by decreased of bone mass density. Its incidence is higher in older population.Serum osteocalcin level is related to the rate of bone turnover. To analyze serum osteocalcin level on osteopenic and osteoporosis subjects.We examined 61 subjects for detecting osteopenia and osteoporosis by Sahara densitometer and measuring serum osteocalcin level byElecsys N-MID Osteocalsin Assay test. Of 21 men and 40 women, prevalence of osteopenia and osteoporosis were higher in women(57.5% and 32.5%) than men (42.9% and 23.8%). Most of osteoporosis subjects were in ≥ 60 years group and osteopenic subjects werein 50–59 years group (p<0.05). Mean of serum osteocalcin on osteoporosis subjects (26.70 ng/mL) was higher than osteopenic (23.78ng/mL) and normal (19.16 ng/mL) subjects (p<0.005). Prevalence of osteopenia and osteoporosis were higher in women than men. Theincidence of osteoporosis was related to the increase of age. Serum osteocalcin level was related to the severity of diagnosis category.
KADAR KREATININ DAN BERSIHAN KREATININ PENDERITA LEPTOSPIROSIS Ismawati Amin; B. Rusli; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 2 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i2.882

Abstract

Leptospirosis is a frequent zoonosis in the tropics including Indonesia with 5–30% incidence and 5–20% mortality rate due toacute renal failure (ARF). To determine the frequency of leptospirosis by sex, age and glomerulus’s filtration rate (GFR) of the patient.A retrospective descriptive study on 32 subjects was done by measuring the creatinine clearance using Lyasis Auto analyzer in ClinicalPathology Laboratory of Wahidin Sudirohusodo Hospital from January 2004 to June 2006, of 32 patients, 25 (78%) male and 7 (22%)female, with the most frequent age range from 30–40 years (34.4%). Of the patients, 2 (6.3%) have normal GFR, 6 (18.8%) withmild GFR reduction, 10 (31.3%) with medium GFR reduction, 10 (31.3%) with severe GFR reduction and 4 (12.5%) with acute renalfailure. Leptospirosis is most frequent found in male with age between 30–40 years old. with reduction of GFR from mild to severe, andeven terminated with acute renal failure.
GAMBARAN SEROLOGIS IgM – IgG CEPAT DAN HEMATOLOGI RUTIN PENDERITA DBD D. Irwadi; M. Arif; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 2 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i2.860

Abstract

The prevalence of Dengue Hemorrhagic Fever (DHF) in South Sulawesi is still high. Proper and immediate management is importantto reduce the disease morbidity and mortality The aims of this study are to know the between correlate in stages of the disease and valuesof blood IgM-IgG, routine haematology analyses as will as infection status. A cross sectional study was done at Wahidin SudirohusodoHospital Makassar in January – June 2006. The diagnosis of DHF following WHO criteria (1999) and Immunocromatographic unit(ICT) for sera IgM-IGg have been conducted. From 83 collected samples, 37 male (44.6%) and female 46 (55.4%) ranged betweenage 6 months up to 47 year old. The decrease of trombocyte counts (mean 70.120/μL) and increase of (mean 10.09%) were observed.Serological unit result showed 10 (12%) primary infections and 68 (82%) secondary infections. Immunoglobulin G could be detectedin the second day of fever. The samples, 56 (67.5%) were stage I, 15 (18.1%) were stage II, and 12 (14.4%) were stage III or IV, thatmeans there were correlation between infection status and disease’s stages (p<0.01). It can be concluded they routine blood analysisis still useful for diagnosis of DHF, because it was confirmed by rapid serological unit specifically for IgM-IgG in early phase of DHF. Sothey an adequate treatment came out in order to prevent severe case of the disease.
OLD PEOPLE AND DIABETES MELLITUS Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.842

Abstract

Menjadi tua adalah proses mengembangkan hidup yang berkaitan dengan kumpulan perubahan yang di orang lanjut usia (lansia)dapat meningkatkan resiko kematian. Sindroma metabolik merupakan salah satu perubahan tersebut yang dikenal secara klinis danhasil laboratorik yaitu obesitas, hipertensi, intoleransi glukosa, dislipidemia, proinflammatory dan prothrombotic state. Hal ini dapatmeningkatkan prevalensi diabetes mellitus tipe 2 (T2DM) dan komplikasinya. Karena banyak kasus DM tidak terdiagnosis, penyaringan(skrining) yang diikuti pencegahan dan penatalaksanaan sangat diperlukan. Menyaring (skrining) orang lanjut usia dengan satu ataulebih komponen sindroma metabolik yang belum terdiagnosis DM dengan menilai glukosa darah puasa dan dua jam sesudah bebanuntuk diagnosis DM dan resiko kematiannya. Penyaringan (skrining) dilakukan di 1080 lansia umur 50 tahun atau lebih di Makassaryang diduga DM dengan tes glukosa plasma puasa dan dua jam sesudah beban, kemudian dinilai menurut klasifikasi WHO 1998.Dari 1080 pasien yang terdiri atas 560 (51,85%) laki-laki dan 520 (48,15%) perempuan, ditemukan DM, 420 (38,89%) denganglukosa plasma puasa  126 mg/dl, 425 (39,35%) dengan 2 jam sesudah beban glukosa  200 mg/dl dan 517 (47,87%) denganglukosa plasma puasa  126 mg/dl serta 2 jam sesudah beban  200 mg/dl. Satu atau lebih komponen sindroma metabolik orangtua merupakan biomarker atau prediktor untuk T2DM dan PJV. Pemeriksaan kesehatan secara reguler atau sedikitnya tes gula darahpuasa dan 2 jam sesudah beban glukosa di orang tua perlu dilakukan dan termasuk dalam sistem asuransi kesehatan. Diagnosis dinidiabetes dan penatalaksanaannya berarti mengurangi komplikasi, cacat, kematian maupun pembiayaan.
PROFIL TES DARAH RUTIN DAN JUMLAH LIMFOSIT TOTAL PADA PENDERITA HIV/AIDS Amraini Afiah; M. Arif; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 2 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i2.883

Abstract

CD4 test and Viral Load have been done for initiating, controlling, and deciding the treatment and prognosis of HIV/AIDS.If bothtests cannot be done, clinical symptoms and total lymphocyte count (TLC) can be used. TLC and Hb can be used for monitoring theHIV progressiveness. To evaluate routine blood test results, TLC, and CD4 of HIV/AIDS patients. Cross sectional and descriptive studyfrom October 2005 to April 2006, of 30 patients, i.e. 21 men (70%) and 9 women (30%). Leucopenia was found in 16.67% men and13.33% women. Anemia Hb in 70% men and 30% women with mean value 2.52 g/dl; lymphocytopenia in 46.67% men and 10%women; and netropenia in 16.67% men and 10% women. CD4 was discovered decline in 66.67% men and 30% women with mean value63.4 cell/mm3. TLC in 43.33% men and 16.67% women with mean value 1081.21 cell/mm3. In the study we found i.e. leucopenia,anemia, limfocytopenia, and neutropenia. CD4 and TLC are mostly decline. Further researches in the bigger populations are conductedto consider the parameter of routine blood tests, TLC, and CD4 on HIV/AIDS patients for initiating therapy.
ANALISIS KADAR SERUM FERITIN DI KARSINOMA PAYUDARA (Analysis of Ferritin Levels in Carcinoma Mammae) Sriwati Atjo; Uleng Bahrun; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 1 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i1.1219

Abstract

Carcinoma Mammae is a malignant disease originating from mammary parenchyma, and the second largest cause of death inthe world. Ferritin is a marker of neoplasia, which levels are increased in non-metastatic carcinoma mammae and more increased inmetastatic carcinoma mammae. This cross sectional study was conducted during June 2012 in carcinoma mammae patients of theWahidin Sudirohusodo Hospital, Ibnu Sina Hospital and Labuang Baji Hospital in Makassar. The aim of this study was to know theserum ferritin levels in metastatic and non-metastatic carcinoma mammae. In this study, 56 samples were obtained and grouped intometastatic and non-metastatic carcinoma mammae patients based on the Tumor, Node, Metastasis (TNM). Ferritin test was conductedby ECLIA method using Elecsys Analyzer Kit 2010 (Roche, USA). The study showed that the average ferritin concentration in advancedstage of carcinoma mammae (155.45 ng/mL) was higher than in the early stage (82.74 ng/m). The Mann Whitney test showed significantdifferences between the early and advanced stage (p=0.01), the median ferritin value in metastatic carcinoma mammae was higherthan non-metastatic (79.85 ng/mL). The Mann Whitney test showed significant differences between metastatic and non - metastaticcarcinoma mammae patients (p=0.00). Based on this study, it can be concluded, that ferritin levels can be used as a biomarker to predictthe progressivity of carcinoma mammae.
RESISTENSI Mycobacterium Tuberculosis TERHADAP OBAT ANTI TUBERKULOSIS A. Nikmawati; Windarwati Windarwati; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.843

Abstract

Patients infected with resistant Mycobacterium tuberculosis strain will be very difficult to cure by standard treatment. To evaluatethe drug resistance of Mycobacterium tuberculosis. A Cross sectional study was performed from January until July 2005. Samples wereobtained from sputum of the suspect tuberculosis. All samples were cultured in Lowenstein-Jensen Media and followed by sensitivity testaccording to resistance ratio method. Of 236 samples, there were 30% positive cultures. The percentage of mono-resistance to Isoniazidwas 70%, to Rifampicin 64.3%, to Ethambutol 62.8% and Streptomycin 64.3%. The percentage of Multi Drug Resistant Tuberculosis(MDR-TB) was 20–40% and the percentage of poly-resistant (Ethambutol and Streptomycin) was 47.1%. The percentage of suspecttuberculosis with positive culture was 30%. There were also found high percentage of mono-resistant, poly-resistant and MDR-TB.
ANALISIS KADAR ALBUMIN SERUM DENGAN RASIO DE RITIS PADA PENDERITA HEPATITIS B AT. Lopa; B. Rusli; M. Arif; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 2 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i2.884

Abstract

Hepatitis is a inflammation process which may present in an acute or chronic phase. A decrease of serum albumin level may befound in liver disease. Ratio de Ritis in mild hepatitis is < 1 while in alcoholic hepatitis is >1. To analyze serum albumin level withratio de Ritis in hepatitis B patients. A cross sectional study was carried out comprising 46 subjects from October 2005 to August 2006measuring albumin level SGOT and SGPT levels using Lyasis autoanalyzer. Thirty eight males and eight females, the level of serumalbumin decreased with an average of albumin level 2.98 gr/dL (p<0.05). Twenty two subjects showed a ratio de Ritis ≤ 1 with theaverage albumin level 3.00 gr/dL and 24 subjects were included in group ratio de Ritis > 1 with average albumin level 2.96 gr/dL(p value = 0.658). There was a decrease of serum albumin level in hepatitis B patient but no significant difference between decrease ofserum albumin level with ratio of de Ritis ≤ 1 and ratio of de Ritis >1.
Co-Authors A. Nikmawati Amraini Afiah Ani Kartini AT. Lopa B Rusli B. Rusli D. Irwadi Darwati, Darwati Didi Irwadi Elisabeth Frida Fitriani Fitriani Ismawati Amin M Arief M Arif M. Arif Mansyur Arif Mutmainnah Mutmainnah N Sennang AN N. Ibrahim Nurahami Nurahmi R A Nawawi RDN Pakasi Rostina Rostina S Aprianti S Josten S. Ibrahim Samirah Samirah Sriwati Atjo Suci Aprianti Sulina Y. Wibawa Syafii Syafii T Esa Uleng Bahrun Windarwati Windarwati