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Journal : Proceedings of Annual International Conference Syiah Kuala University - Life Sciences

Growth and proline content of potato Solanum tuberosum L. in vitro candidate tolerant to drought of origin callus Bilter A. Sirait; Iovie P. Roselyna; . Charloq
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Screening of candidate tolerant potato (Solanum tuberosum L.) to drought in vitro from callus was aimed to determine the growth and proline content of potato plants. This research was conducted at the Laboratory of Tissue Culture of Dinas Pertanian of North Sumatra Province, Jl Karya Jasa No 6 Gedung Johor Medan, in May 2012 until May 2013. The initial research was callus formation followed byorganogenesis. Then, was treated by PEG, 1000, 2000 and 3000 ppm. The result indicated that increased concentration of PEG decreased the amount of leaves and number of internodes. The higher the PEG concentration, the higher the proline content of potato in vitro.
Study of early screening of potato (Solanum tuberosum L.) as a result of drought stress (in vitro) . Charloq; Ernitha Panjaitan; Bilter A. Sirait
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Preliminary studies in Screening of Potato (Solanum tuberosum L.) as a result of drought stress (in vitro). This study aim was to determine the limits of potato (Solanum tuberosum L.) to drought stress conditions in vitro using PEG. This research was conducted at the Tissue Culture Laboratory Unit / Laboratorium Kultur Jaringan UPT/Balai Benih Induk Hortikultura Dinas Pertanian Propinsi Sumatera Utara, Jl. Karya Jasa No. 6 Gedung Johor Medan in March 2012 to June 2012. The Completely Randomized Design (non factorial) was used with PEG concentrations of five levels, namely: P1 = 10%, P2 = 20%, P3= 30%, P4 = 40% and P5 = 50% with 5 replications. The results showed that increasing the concentration of PEG will reduce the percentage of plantlets survival, reduce plant height, number of roots and number of nodes. Treatment of 50% PEG resulted in the death of potato cuttings due to drought stress.