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Studi Bioekologi Parasitoid Tetrastichus brontispae Feer (Hymenopters : Eulophidae) Terhadap Hama Brontispa longissima Gestro (Coleoptera:Chrysomelidae) Maria Goreti Nere; Yustina Maria Silvia Wonga Puu
AGRICA Vol. 4 No. 1 (2011): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v4i1.468

In recent years, the coconut leaf beetle (Brontispa longissima, Hymenopters: Eulophidae) has been a significant pest in Ende, Indonesia and has destroyed the majority of the coconut crop leading to overall production to decline. In 2007 coconut leaf beetle began being controlled biologically using the parasitoid Tetrastichus brontispae (Hymenopters: Eulophidae). This is now considered locally to be the most effective way to suppress pest populations of coconut leaf beetle but until now there have been no scientific studies on this biological pest control method. This study aims to determine the effectiveness of Tetratichus brontispae parasitoids as a treatment for coconut leaf beetle infestations. This research used a Completely Randomized Design (CRD), which was comprised of three types of experiments. First, the average coconut leaf beetle survival rate was measured when exposed to T. brontispae at five ratios: 1:1, 2:1, 3:1, 4:1, and 5:1. Researchers also measured the effect of Tetratichus brontispae treatment duration using 5treatments, namely: 24 hours, 48 hours, 72 hours, 96 hours, and 120 hours of repeated 10 times. 3) Observation of biological parameters of Tetratichus brontispae was conducted qualitatively. The results showed that treatment with the highest level of parasitization occurred at the ratio of 3:1, as much as 20.75% parasitization, and the lowest level parasitization effect occurred at a level of the 1:1 ratio, as low as 7.07%, with the highest percentage survivability of T. Brontispae occurring on the first day (6.48%) and the lowest on day five at 1.29%. On average, 98.13% of T. Brontispae specimens produced offspring, and an average of 32.71% of specimens gave birth each day. Tetratichus brontispae adults had an average life span of 9-11 days, had no pre- oviposition period with the oviposition period occurring until day five. Specimens would die an average of 6 days following oviposition.

IDENTIFIKASI HAMA PENGGEREK DAUN Liromiza spp. (Diptera: Agromyzidae) DAN PARASITOIDNYA YANG BERASOSIASI PADA BERBAGAI JENIS TANAMAN SAYURAN DI KECAMATAN DETUSOKO DAN KELIMUTU KABUPATEN ENDE Sri Wahyuni; Yustina Maria Silvia Wonga Puu; Kristina Erniyani; Julianus Jeksen
AGRICA Vol. 1 No. 1 (2008): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v1i1.475

The aim of this research is to identify Liromiza spp. And the parasitoid is associated with various types of vegetable plants in Detusoko and Kelimutu Districts in Ende Regency. Data were analyzed by matching morphology of existing specimens with insect morphology books written by Bhorror 1970 and matching with standard specimens originating from Bali and Bogor. The results obtained from two vegetable producing districts in Ende, namely Detusoko and Kelimutu, show the following: There are two types of Liriomyza spp associated with several types of host plants in Ende Regency. L. huidobrensis (Diptera: Agromyzydae) is located in Kelimutu District and L Sativae (Diptera: Agromyzydae) is in Detusoko District. The diversity of parasitoids found in Detusoko Subdistrict were as many as four species from the Eulopidae family while in Kelimutu District there were five types of parasitoids originating from two families namely Braconidae and Euiopidae. Neosacharis okazaki is the most dominant type of parasitoid in each plant in Detusoko District. Opiits sp dominant in Kelimutu Subdistrict on the long bean, green beans, mustard greens, chicory and cabbage. While H. varicornis is dominant in Kelimutu District in tomato plants.

INVENTARISASI HAMA PASCAPANEN JAGUNG DALAM PENYIMPANAN DI TINGKAT PETANI DAN PEDAGANG DI KUPANG Yustina Maria Silvia Wonga Puu; Samuel Pakan; Lince Makkun
AGRICA Vol. 1 No. 1 (2008): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v1i1.478

This research was conducted at pest and plant disease laboratory, faculty of agriculture Nusa Cendana University in Kupang. Corn sample is taken from fanner in Amarasi, Kupang Timur, Kupang Barat and from the trader in Oesapa, Oesao, Penfui. This research intends to inventory pest after yields corn and to know the damage level of corn in farmer and trader. This research used a 32 kg corn sample for quotation methods. The observed variable is pest insect species, imago population, damage seed percentage, Weight decrease percentage, water level, temperature and moisturizer. Pest insect population and species observed on 1 kg corn. While seed damage and weight decrease per cent on 100 g corn seed and 5 g for water level. Data result tabulated and descriptively analyzed. The result of the research showed 1 pest insect species in the farmer corn storage is S.zeanmys and 2 species in trader corn storage is S.zeanmys and T confusum. Highest population S.zeamays founded in Penfui trader by 50,50/kg and lowest population 4,50/kg in farmer at Amarasi. T.confusum population only founded in trader not in farmer level. Highest population 2,17/kg in Penfui trader and lowest 0,17/kg in Oesapa trader. While highest seed damage percentage founded in Penfui trader by 11,13% and lowest 1,22% in farmer at Amarasi and highest weight decrease percentage founded in Oesapa trade by 0,77% and lowest 0,25% in farmer at Kupang Barat

STRUKTUR KOMUNITAS MESOFAUNA TANAH PEROMBAK BAHAN ORGANIK PADA VEGETASI KOPI DAN KAKAO Kristina Erniyani; Sri Wahyuni; Yustina Maria Silvia Wonga Puu
AGRICA Vol. 3 No. 1 (2010): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v3i1.488

The use of inorganic fertilizers and pesticides can change the agroecosystem gradually. These changes may result in a decrease in the diversity of soil organisms. These organisms are very important in the decomposition process of reforming the organic matter into compounds that are readily absorbed by plants. Other changes include a change in the soil structure which will affect the soil chemical and physical characteristic and ultimately affect the soil productivity leading to a decreased agricultural product. The return of the remains from harvest, litter and crop residues from coffee and cocoa crops to the soil will provide energy for soil organisms and it will help maintain the soil environment that supports the life of other organisms. This research aimed at identifying the composition and diversity of mesofauna formed by the composted organic matter in cocoa and coffee plantations. Soil sampling was conducted on coffee and cocoa plantations in the Onelako village of Ndona District. Soil sampling was conducted in September 2010. Soil sampling used Neuman method of 0-15 cm and 16-30 cm depth. Each soil sample was taken to the laboratory. The separation of mesofauna from soil used Berlese-tullgran method that had been modified for 4 days. The separated Meso fauna was identified under the light microscope. The result of this research showed that the highest composition of mesofauna decomposer of organic matter in cocoa and coffee vegetation is Collembola. Collembola is very tolerant to acidic soil condition and they are dominant in the soil and surface habitats that contain lots of litter. Collembola is microarthropods that have large distribution both in quantity and diversity. The highest diversity of mesofauna on coffee vegetation is 1.525 and on cocoa, vegetation is 1.273. It was also noted that at a depth of 0-15 cm there was a higher concentration of mesofauna in the coffee vegetation area, which was about 7.3960, while in the cocoa vegetation there where 3.6376. The high level of the diversity index caused an increase in decomposition and therefore better soil fertility.

EFEKTIVITAS CENDAWAN ENTOMOPATOGEN BEAUVARIA BASSIANA TERHADAP HAMA PENGGEREK BUAH KAKAO CONOPOMORPHA CRAMERELLA SNELLLEN Yustina Maria Silvia Wonga Puu
AGRICA Vol. 3 No. 1 (2010): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v3i1.492

Cocoa pod borer Conomorpha cramerella snellen (lepidoptera: gracillaridae) is one type of the key pest which is highly responsible for drilling cocoa, which then causes the production and quality of cacao to decrease. The use of entomopathogenic fungus is one of the alternatives to cope with it. By invecting and developing within the insect, such a fungus can cause the insect to suffer from diseases which will then be responsible for their death. Beauveria bassiana are types of the entomopathogenic fungus which are used for controlling cocoa pod corer, as they are able to infect the pest trough the enzyme or toxin produced, which then leads to its death. This study aims at an effectiveness test of entomopathogenic fungus Beauveria bassiana against cocoa pod borer. This research was conducted at the Laboratory Udaya University in Bali from May to November 2010. The experimental design employed was randomized complete block design (RCBD) wich was made up of four treatments such as B0 (Control), B1 (B. bassiana in which the spore density was 105 spora/ml/); B2 (B, bassiana in which the spore density 106 spora/ml); B3 (B. bassiana in which the spore density was 107 spora/ml). The result shows that the Larvae CPB which was infected by B. bassiana shows different treatments and responses than control. The fastest death of the larvae CPB took place on the treatments and responses than control. The fastest death of the larvae CPB took place on the treatment of B. bassiana in which spore density 107. The fastest appearance of spore took place on the treatment five days after inoculation. The fungus of B. bassiana at the spore speed of 105 and 107 caused all the larvae CPB 100% to die at five days inoculation.

PENYEBARAN HAMA KUTU PUTIH PEPAYA PARACOCCUS MARGINATUS Williams & Granara de Willink (Hemiptera : Pseudococcidae) PADA BERBAGAI TANAMAN INANG DI KABUPATEN ENDE Yustina Maria Silvia Wonga Puu
AGRICA Vol. 3 No. 2 (2010): December
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v3i2.495

Papaya is one of the many horticultural commodities grown by farmers. In mid-2008, thousands of trees in the District Sukaraga papaya, Bogor Regency pest infestation papaya white and whole tree has been attacked. P. marginatus is a prolific insect that attack several host plants, including tropical fruit crops and ornamental plants. This research aims to determine the spread of lice infestation in White Paracoccus marginatus Ende and type of host plants are attacked by pest Mite White Paracoccus marginatus. This study is useful to obtain information about the spread of pest P. marginatus in the district of Ende as a step in taking control measures, information to relevant agencies and farmer about the existence pf pest P. marginatus in Ende district, and continued research on the existence of natural enemies and pest bioecology O. Marginatus. Sampling study conducted in 21 sub-district in NTT Propinsis Ende. The study was conducted from March-August 2011. Materials used in this study plant pest Paracoccus marginatus, Alcohol 95%. The tools used are a collection of bottles, brushes, a microscope, transparent plastic, paper labels, Thermo-Hygrometer, coo box, stationery and literature. The result showe that the spread of pest P. marginatus was observed in 21 sub-district in Ende found only in the District 2 District East Ende and Ndona. Papaya is attacked by P. marginatus have chlorotic leaves, young leaves to fall and prospective fruits and fruit and leaves are covered by this pest by stacking layers of wax until the drought ends up dead. The intensity of damage caused by pest P. marginatus in the District of East Ende of 33.33% and 16.67% for Ndona District. The spread of pest P. marginatus in the district of Ende found at two locations namely Ende District East and District Ndona. Types of host plants are attacked by pest P. marginatus is Papaya plants.

UJI AKTIVITAS EKSTRAK DAUN MBOA (Melastomata malabatricum) TERHADAP HAMA GUDANG Callosobruchus sp (COLEOPTERA:BRUCHIDAE) PADA KACANG HIJAU (Phaseolus radiata L.) Hartini Haris; Yustina Maria Silvia Wonga Puu
AGRICA Vol. 3 No. 2 (2010): December
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v3i2.498

Activity Test Extract Mboa Leaf (Melastomata Malabatrikum) against warehouse pest Callosobruchus Sp (Coleoptera : Bruchidae) Green bean (Phaseolus Radiata L.). Green beans are an important crop in the tropics and have long cultivated in Indonesia. Green bean production in quantity and quality is strongly influenced by the presence of pest and diseases. The damage caused by insects ensued can reach 70% (Kartasapoetra, 1987). Given the magnitude percentage damage inflicted by insects CallosobbrushusSp is a necessary control measure. This study aims to determine the activity of leaf extract Mboa (M. Mallabatricum) against warehouse pest Callosobruchus Sp. This study uses a CDR with four treatments (M25, M50, M75, M100) and repeated 10 times. The result showed extra activity Mboa leaves (M. Mallabatricum) against Callosobruchus Sp to test a contact poison can cause Callosobruchus Sp morality above 80% seen in the treatment of M50, M75, and M100 of 9.987%. repellant test showed a decrease traffic to continue successive treatment – participated in treatments M25 (61%), M50 (46%), M75 (43%), M100(34%) and mortality due to neurological disorders for M25 (7.34%), M50 (7.99%), M75 (8.54%), and M100 (9.21%). An antibody test can suppress high levels of oviposition in each treatment. M25 (31.21%), M50 (71.01%), M75(79.5%), and M100 (87.22%).

PEMANFAATAN CENDAWAN ENTOMOPATOGEN Beauveria bassiana (BALSAMO) VUILEMIN DALAM PENGENDALIAN HAMA TANAMAN Yustina Maria Silvia Wonga Puu
AGRICA Vol. 2 No. 1 (2009): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v2i1.545

The using of synthetic pesticide in control of crop pest cause various of negative impacts like resistance, resurgence, the incidence of secondary pets, killing of the natural enemy and is impure of environmental even death to human being and animal. One of the correct operation alternative to decrease the using of synthetic pesticide is biological control with exploiting of entomopathogen caiied Beauveria bassiana. This Entomopatogen has an ability for infection of insect either through direct contact, and also inoculation of woof was given to target insect by producing the toxin so that insect body closed conidia and become hard like a mummy. The ability is also influenced by the condition of environment especially temperature and dampness. B. bassiana has high patogenistas ability almost in all of the insect. B.bassiana can depress the population of coffee powder H. hampei of Helopeltis spp equal to 76% and the other insect type. Application of B. bassiana don't generate death at another pest which good for (natural enemy) in nature. Infection mushroom mechanism of entomopathogenic B. bassiana in control of pest in the field happened by direct contact, inoculation and also contamination with target pest insect and effective in controlling of various pest insect type. Its effectiveness is influenced by a variety of isolate types, the closeness of spore, quality of media grow, controlled pest type, age of stadia pest, application time, application frequency and environmental factor.

PKM-KELOMPOK TANI ALAM SUBUR DI DESA NDUARIA KECAMATAN KELIMUTU KABUPATEN ENDE NUSA TENGGARA TIMUR Yustina Puu; Charly Mutiara; Yohanes Pande
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol 4, No 2 (2019): December 2019
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v4i2.3480

Partners in this PKM is Alam Subur Farmers in Nduaria Village that has fifteen members. Based on the results of a survey conducted, it is known that the problems faced by partners are lack of counseling and assistance to farmer groups in the use of inputs and fertilizers and pesticides, the price of chemical fertilizers and pesticides is very expensive and very complicated to obtain, farmers do not know how to make organic fertilizers and pesticides that use plants around the farmers' land, farmers do not know how to control pests and diseases in cabbage and mustard plants, the statutes and bylaws of the group are not legally strong, and group management is still weak. The solution provided to overcome the problems of partners is to provide knowledge and skills to farmers in this group about the facilities and infrastructure used in aquaculture activities, the correct use of fertilizers and pesticides in aquaculture activities, making organic fertilizers and vegetable pesticides from plants around farmer's land, control of pests and diseases in cultivated plants and good group management by making a statute and by laws. Output targets to be achieved in PKM activities include a scientific article published through an ISSN journal, an article in print media, a video of PKM activity, and farmers can make organic fertilizer and pesticides from plants around their land. The methods that will be used in achieving the above objectives are counseling and training. The results achieved are 90% of farmers have understood and can make organic fertilizers and pesticides. DOI: https://doi.org/10.26905/abdimas.v4i2.3480

Fall Armyworm Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in East Nusa Tenggara Province of Indonesia: Genetic Characterization and Strain Detection Yustina Maria Silvia Wonga Puu; Itji Diana Daud; Andi Nasruddin; Melina Melina
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 2 (2024): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i2.91748

Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) is an invasive pest of corn plants spreading throughout the world, including East Nusa Tenggara (NTT), Indonesia. Despite the wide distribution, there is a lack of information on the strain or genetic diversity of the pest in NTT. Therefore, this study aimed to determine the strain of S. frugiperda from several areas in NTT with a molecular method using cytochrome oxidase subunit 1 (COI) and triose phosphate isomerase (Tpi) gene markers. The samples were collected from 3 islands: Timor, Flores, and Sumba. Amplification of the marker genes was carried out using 3 specific primers to identify the strain obtained from samples. Subsequently, polymerase chain reaction (PCR) products were sequenced and the DNA sequences were analyzed using the BioEdit and BLAST programs. Phylogeny analyses were carried out using the MEGA 11 program to verify the strain group of samples with reference isolates from other countries found in GenBank. The PCR results showed product amplicon size 811 bp for the CO1A marker. Based on phylogeny tree analysis using COI marker, S. frugiperda from NTT showed 2 clades, namely corn and rice strains. The characterization results showed that S. frugiperda in NTT comprised 63.6% corn and 36.4% rice strains. COIB classified S. frugiperda from NTT into the h4 haplotype subgroup, while Tpi gene marker was in the corn strain. This study provided valuable information regarding the strain of S. frugiperda in Indonesia to determine the appropriate control strategy.

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