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All Journal Bioedukasi: Jurnal Pendidikan Biologi Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Penelitian Hayati

Fatimah Fatimah

Departement Of Biology, Faculty Of Science And Technology, Universitas Airlangga, Surabaya, Kampus C, Jl. Dr. Ir. H. Soekarno, Mulyorejo Surabaya, Jawa Timur; University CoE Research Center For Bio-Molecule Engineering, Universitas Airlangga, Kampus

Author-ID : 3543208

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Articles

PELATIHAN PEMBUATAN PUPUK ORGANIK CAIR PADA KELOMPOK TANI DI KABUPATEN PROBOLINGGO Tini Surtiningsih; Fatimah Fatimah; Ni’matuzahroh Ni’matuzahroh; Agus Supriyanto; Tri Nurhariyati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

This community service aims to apply the method of making and liquid organic fertilizer and improving the skills of farmers in making liquid organic fertilizer. In addition to reducing the dependence of farmer groups on inorganic fertilizers and utilizing the results of sugarcane waste, namely molasses and microbes as a formula in the manufacture of liquid organic fertilizer. The method that was carried out was to increase insight into liquid organic fertilizer, and how to make it. The results of the socialization of liquid organic fertilizers showed that the insights of farmer groups increased by an average of 77% through the pretest and post test values during socialization. Based on the evaluation shows that the farmer group has been able to make liquid organic fertilizer independently. The socialization of making liquid organic fertilizer provides additional insight for farmer groups on the use of liquid organic fertilizer for agriculture.AbstrakPengabdian kepada masyarakat ini bertujuan untuk menerapkan cara pembuatan dan pupuk organik cair dan meningkatkan keterampilan petani dalam membuat pupuk organik cair. Selain itu untuk mengurangi ketergantungan kelompok tani terhadap pupuk anorganik dan memanfaatkan hasil limbah tebu yaitu molase dan mikroba sebagai formula dalam pembuatan pupuk organik cair. Metode yang dilakukan adalah peningkatan wawasan mengenai pupuk organik cair, dan cara pembuatannya. Hasil sosialisasi pupuk organik cairmenunjukkan bahwa wawasan kelompok tani meningkat rata-rata sebesar 77% melalui nilai pretest dan post test saat sosialisasi. Berdasarkan evaluasi menunjukkan bahwa kelompok tani telah mampu membuat pupuk organik cair secara mandiri. Sosialisasi pembuatan pupuk organik cair memberikan tambahan wawasan bagi kelompok tani terhadap pemanfaatan pupuk organik cair bagi pertanian.

PELATIHAN PENGOLAHAN LIMBAH TOILET MENGGUNAKAN FORMULA MIKROBA DI PERUMAHAN TENGGILIS SURABAYA Fatimah Fatimah; Tini Surtiningsih; Agus Supriyanto; Nima’tuzahroh Nima’tuzahroh; Salamun Salamun; Tri Nurhariyati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Blockage of septic tanks is one of the problems in the community related to the discharge of toilet waste produced. The use of synthetic toilet cleaners that are not environmentally friendly is one of the factors that can inhibit the process of decomposing organic waste. The existence of microbial killer ingredients in toilet cleaning materials that come into the septic tank will actually inhibit the growth of essential microbes that play a role in the perfection of the decomposition process. Lack of understanding of the community related to microbial processes in the processing of toilet waste is one of the factors inability of the community to overcome these problems. This community service aims to disseminate information related to the processing of organic waste, the role of microbes in the processing of toilet waste, how to make microbial formulas that play a role in the processing of toilet waste, as well as efforts to increase the creativity of the community to process their own waste to improve environmental sanitation and improve community welfare. The results of the training showed that the average pretest and post test scores of the cadres of Tenggilis Surabaya village experienced a significant increase in value with a mean value of 37.78 (pretest) to 62.22 (post-test). Toilet waste processing cadres have been able to make biotoilet products that can be used to process limbat toilets in their homes. The informationand insights they receive can be passed on to the community from each cadre.AbstrakPenyumbatan septic tank merupakan salah satu permasalahan di masyarakat terkait dengan buangan limbah toilet yang dihasilkan. Penggunaan pembersih toilet yang berbahan sintetik yang tidak ramah lingkungan merupakan salah satu faktor yang dapat menghambat proses penguraian limbah organik. Keberadaan bahan pembunuh mikroba dalam bahan-bahan pembersih toilet yang ikut masuk ke dalam septik tank justru akan menghambat pertumbuhan mikroba esensial yang berperan dalam kesempurnaan proses dekomposisi. Kurangnya pemahaman masyarakat terkait dengan proses mikroba dalam pengolahan limbah toilet merupakan salah satu faktor ketidakmampuan masyarakat dalam mengatasi permasalahan tersebut. Pengabdian masyarakat ini bertujuanuntuk mensosialisasikan informasi terkait proses pengolahan limbah organik, peran mikroba dalam pengolahan limbah toilet, cara membuat formula mikroba yang berperan dalam pengolahan limbah toilet, serta upaya meningkatkan kreativitas masyarakat untuk mengolah limbahnya sendiri guna meningkatkan sanitasi lingkungan dan meningkatkan kesejahteraan masyarakat. Hasil pelatihan menunjukkan bahwa rerata nilai pretest dan post test kader kelurahan Tenggilis Surabaya mengalami peningkatan nilai yang cukup signifi kan dengan rerata nilai dari 37,78 (pretest) menjadi 62,22 (post test). Kader pengolahan limbah toilet telah mampu membuat produk biotoilet yang dapat dimanfaatkan untuk mengolah limbat toilet di rumah masing-masing. Informasi dan wawasan yang mereka terima dapat di teruskan kepada komunitas asal masing-masing kader.

PENINGKATAN PRODUKTIVITAS KELOMPOK SANTRI DALAM BUDIDAYA JAMUR TIRAM PUTIH DI PONDOK PESANTREN DARUL HUDA, JABON, SIDOARJO Nima’tuzahroh Nima’tuzahroh; Fatimah Fatimah; Nur Indradewi Oktavitri
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Islamic boarding schools generally have human and natural resources, and have a large influence on the communities around them. These resources can be optimized to increase students’ productivity with White Oyster Mushroom (JTP) cultivation. This community service aims to implement JTP cultivation to increase students’ productivity. The method that was carried out was to increase the knowledge of JTP cultivation, the formation of JTP cultivation cadres for making kumbung, JTP cultivation, JTP harvesting and sales. The JTP results show that the students’ insight increased by an average of 73% through the pretest and posttest scores during socialization. 3 Cadres are formed from coordination with the head of the cottage. The location chosen for mushroom cultivation is based on the criteria for JTP cultivation. JTP cultivation cadres have been able to make 4 x 6 m mushroom kumbung around the hut. Cultivation is done using 2000 baglog mushrooms for 1 month. Increased net income from santri through harvesting mushrooms from Rp. 0 to Rp. 4,000,000 every 2000 baglog after harvest. This increase in income can show an increase in santri productivity for income for Islamic boarding schools.AbstrakPondok pesantren umumnya memiliki sumber daya manusia dan sumber daya alam, dan memiliki pengaruh besar terhadap masyarakat di sekitar mereka. Sumber daya tersebut dapat dioptimalkan untuk peningkatan produktivitas santri dengan budidaya Jamur Tiram Putih (JTP). Pengabdian masyarakat ini bertujuan untuk penerapan budidaya JTP untuk peningkatan produktivitas santri. Metode yang dilakukan adalah peningkatan wawasan budidaya JTP, pembentukan kader budidaya JTP untuk pembuatan kumbung, berbudidaya JTP,pemanenan dan penjualan JTP. Hasil JTP menunjukkan wawasan santri meningkat rata-rata sebesar 73% melalui nilai pretest dan post test saat sosialisasi. 3 Kader dibentuk dari koordinasi dengan ketua pondok. Lokasi yang dipilih untuk budidaya jamur berdasarkan kriteria untuk budidaya JTP. Kader budidaya JTP telah mampu membuat kumbung jamur dengan ukuran 4 × 6 m di sekitar pondok. Budidaya dilakukan dengan menggunakan 2000 bag log jamur selama 1 bulan. Peningkatan penghasilan bersih dari santri melalui panen jamur dari semula Rp. 0 menjadi Rp. 4.000.000 tiap 2000 baglog setelah panen. Peningkatan penghasilan ini dapat menunjukkan peningkatan produktivitas santri untuk pemasukan bagi pondok pesantren.

The potency of Micrococcus sp. L II 61 bacteria as oil sludge cleaning agent Ni’matuzahroh Ni’matuzahroh; Intan Ayu Pratiwi; Tini Surtiningsih; Fatimah Fatimah; Sri Sumarsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

This research aimed to reveal the ability of Micrococcus sp. L II 61 bacteria that was isolated from Pegirian Surabaya as oil cleaning agent. This is an experimental research to detect the presence of biosurfactant and lipase enzyme in culture supernatant of Micrococcus sp. L II 61 with aliphatic hy-drocarbon (cooking oil) as a substrate growth. Biosurfactant production was evaluated by measuring the surface tension of supernatant using tensiom-eter du Nouy and measuring the emulsification activity value using diesel oil as hydrocarbon test. Lipase enzyme was detected by measuring lipolitic activity value of crude enzyme (culture supernatant) by using p-nitrofenil palmitic (p-npp) as a substrate test. Data were analyzed descriptively. The results showed that Micrococcus sp. L II 61 produced biosurfactant with surface tension decreasing of culture supernatant up to 30.27 ± 1.17 mN/m compared than aquadest and value of hydrocarbon emulsification activity (AE 1 hour) up to 20.24 ± 0.68 %. Culture of Micrococcus sp. L II 61 after 16 hours incubation have a lipolytic activity 33.53 ± 0.14 U/mL at pH 7 and 37 oC. Supernatant of Micrococcus sp. L II 61 100% (v/v) give the high-est percentage of oil sludge solubility, i.e. 86.38 ± 2.39%. Micrococcus sp. L II 61 is a highly potential to be developed as oil sludge cleaning agent.

STUDI KINETIKA PRODUKSI BIOSURFAKTAN Bacillus subtilis 3KP PADA SUBSTRAT MOLASE Ni’matuzahroh; Nur Hidayatul Alami; A. Faiz Khudlari T.; Fatimah; Tri Nurhariyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/281

Bacillus subtilis 3KP was a bacteria that can produce biosurfactant, when it was grown on a molasses substrate. This research was aimed to know the kinetic type of biosurfactant production, so it can be known the efficiency of molasses and the optimal culture condition to produce biosurfactant. B. subtilis 3KP was grown on mineral salt medium that had been already added with various molasses concentrations (1, 2, 3, and 4 g/l). The culture was incubated until 7 days on a shaker at 27° C and 120 rpm. The measurement of bacterial growth (CFU/ml), pH culture changing, and biosurfactant production which was indicated from emulsification activity and crude biosurfactant weight was determined at various incubation periods (0, 1, 2, 3, 4, 5, 6 and 7 days). Emulsifier activity (E1h) was measured by adding 1 ml of kerosene to 1 ml of biosurfactant supernatant. The measurement was made after 1 hour. The E1h index was given as percentage of height of emulsified layer (cm) divided by total height of the liquid column (cm). Crude biosurfactant was got from ammonium sulfate precipitation, then was dried and was determined in (g/l culture). The data of growth and biosurfactant production were analysed descriptively to know differentiation of biosurfactant production type of B. subtilis 3 KP in that different treatment. The examination of kinetics parameters such as product yield (Yp/x) and specific rate of culture (μ) were taken into account to evaluate a high efficient production process. The results of this research indicated that biosurfactant of B. subtilis 3KP was begun produced at exponential phase and was optimal at the end of exponential phase (production under growth-limiting conditions). Molasses concentration of 3% and 4 days incubation gave an optimal biosurfactant production, so it can be became the exact combination alternative to scale up biosurfactant of B. subtilis 3KP.

UJI PRODUKSI BIOSURFAKTAN OLEH Pseudomonas sp. PADA SUBSTRAT YANG BERBEDA Fatimah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/351

Biosurfactant, microbial metabolite whose properties like surfactant, was suggested to replace chemically synthesized surfactant for take in hand environtmental pollution by petroleum hydrocarbon. This work was done to examine potency of Pseudomonas sp. isolated from Tanjung Perak Harbor to produce biosurfactant. Also, to know the effect of different substrates (glucose + yeast extract, lubricating oil and hexadecane) toward biosurfactant production. Pseudomonas sp. grown in mineral synthetic water and biosurfactant production was measured on stationary phase. Biosurfactant production based on emulsification activity and surface tension reduction of supernatant (using Du Nouy tensiometer). Solar, lubricating oil, and hexadecane were used to examine emulsification activity. Results indicated that Pseudomonas sp. have a potency to produce biosurfactant. Surface tension of supernatant decreased up to 20 dyne/cm, when grown on hexadecane substrate. Hexadecane is the best growing substrate for biosurfactant production than others.

Uji Toleransi Logam Berat Bakteri Hidrokarbonoklastik dan Uji Kemampuan Micrococcus sp. LII61 dalam Menurunkan Kromium (Cr VI), Tembaga (Cu II), Seng (Zn II) Achmad Zainal Abidin; Elga Renjana; Fatimah Fatimah; Ni’matuzahroh Ni’matuzahroh
Bioedukasi: Jurnal Pendidikan Biologi Vol 12, No 1 (2019): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

This research aimed to know: (1) tolerance level of hydrocarbon-degrading bacteria collected from Biology Laboratory of Airlangga University i.e. Acinetobacter sp P2(1) Bacillus subtilis 3KP, Micrococcus sp. LII61, Pseudomonas putida T1(8), at various concentration of heavy metal chromium (Cr6+), copper (Cu2+), zinc (Zn2+), and combination of that three metals; (2) growing ability of selected bacteria in lowering metals concentration; and (3) reduction percentage of metal concentration by selected bacteria. Tolerance test was performed using streak plate method on two types of solid mediums, i.e. Mineral Salt Medium (MSM) agar and Mueller-Hinton Agar (MHA) with a stratified concentration and 72 hours of incubation time. Bacterial growth and metal concentration reduction test were carried out in liquid medium containing single metal or combined metals. Viability of bacteria was calculated using Total Plate Count (TPC) method. Logarithmic of TPC were analyzed statistically using Brown-Forsythe test followed by Games-Howell. The results showed: (1) All bacteria was resistant to either single heavy metal or combined metals on both medium. However, Micrococcus sp. LII61, had the highest tolerance compared to the other three bacteria, which was able to grow at Zn+2 1000 ppm in both medium. Micrococcus sp. LII61, was the selected bacteria to be tested for its ability to reduce heavy metal by single heavy metal and combined metals for 3 and 7 days of incubation time. (2) Ability levels of Micrococcus sp. LII61, to grow in some heavy metals was Zn2+ > Cu2+ > Cr6+ consecutively. (3) Ability levels of Micrococcus sp. LII61, in reducing heavy metals was Cu2+ > Zn2+ > Cr6+ consecutively. Percentage decrease of Cu2+ in single metal was 66.15%, while in combination metals was 54.25% at 3 days incubation. Percentage decrease of Cu2+ after seven day incubation in single metals and combination metals were 90.13% and 74.72% respectively.

Co-Authors A. Faiz Khudlari T. Achmad Zainal Abidin Agus Supriyanto Elga Renjana Intan Ayu Pratiwi, Intan Ayu Nima’tuzahroh Nima’tuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Nur Hidayatul Alami Nur Indradewi Oktavitri Salamun Salamun Sri Sumarsih Tini Surtiningsih Tri Nurhariyati

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