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All Journal Medical Journal of Indonesia Jurnal Wicara Desa
Susan Rahayu
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences

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Establishment of human sperm-specific voltage-dependent anion channel 3 recombinant vector for the production of a male contraceptive vaccine Asmarinah, Asmarinah; Bachtiar, Endang W.; Malik, Amarila; Rahayu, Susan
Medical Journal of Indonesia Vol 21, No 2 (2012): May
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (904.243 KB) | DOI: 10.13181/mji.v21i2.479

Abstract

Background: The aim of this study was to construct a recombinant vector of human sperm specific VDAC3 gene for production of VDAC3 antibody, which is potential as male contraception vaccine.Methods: Target fragment sequence of VDAC3 gene was obtained through amplification of human sperm VDAC3 cDNA with primers covering exon 5 to exon 8. Its PCR product in size of 435 bp was cloned to the pET101/D-TOPO expression vector (5753 bp). E. coli bacteria were transformed with this vector. Cloning of VDAC3 fragment gene to the vector was confirmed by the using of XbaI restriction enzyme and PCR colony method with primers covering exons 5-8 of the human VDAC3 gene.Results: Alignment analysis of amplified fragment covering exon 5 to exon 8 of VDAC3 gene showed 94% homology to human VDAC3 gene from databank. After cloning to the expression vector and transformation to E. coli competent cells, twelve colonies could grow in culture media. Gel electrophoresis of sliced VDAC3 recombinant vector showed a single band in the size of 6181 bp in 8 colonies. After application of PCR colony and amplicon sequencing, the result showed a single band in the size of 435 bp and fragment sequence with 94% identity to human VDAC3 gene.Conclusion: The construction of human sperm specific VDAC3 gene recombinant vector was established in this study. In the future, this recombinant vector will be used to produce VDAC3 antibody for the development of a male contraception vaccine. (Med J Indones. 2012;21:61-5)Keywords: Contraception, recombinant vector, sperm, VDAC3
CARA PEMBUATAN PUPUK KOMPOS BERBAHAN DASAR KOTORAN SAPI Daffa, Ady Ahmad; Astuti, Mustika Fuji; Parastining, Sesilia Novia Ayu; Safitri, Ajeng Aissah; Alvian, Alvian; Rahmawati, Mila; Anaputri, Lesi; Aranta, Muhammad Antony; Ramdani, Suri; Rahayu, Susan
Jurnal Wicara Vol 2 No 5 (2024): Jurnal Wicara Desa
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/wicara.v2i5.5574

Abstract

Making organic fertilizer based on cow dung as an environmentally friendly alternative for modern agriculture. The organic fertilizer produced not only functions to increase fertility land, but also helps manage waste and protect the environment. Making process Fertilizer involves several important stages, such as preventing ingredients, mixing, controlling humidity, aeration and cooling. Studies conducted show that organic fertilizers The resulting product contains good nutrients, provides physical, chemical and chemical improvements biology in soil. This product supports sustainable agricultural practices as well as produce healthier food.
Co-Authors Agnes Stephanie Harahap Alvian, Alvian Amarila Malik Anaputri, Lesi Aranta, Muhammad Antony Asmarinah Asmarinah Astuti, Mustika Fuji Daffa, Ady Ahmad Endang W. Bachtiar Ham, Maria Francisca Parastining, Sesilia Novia Ayu Rahmawati, Mila Ramdani, Suri Safitri, Ajeng Aissah Salinah