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Methylation Profile of HOXA 11 Gene in Eutopic Endometrium on Endometriosis Patient with Infertility Natadisastra, Muharam; Yuwono, Valencia; Febri, Ririn; Asmarinah, Asmarinah
Indonesian Journal of Obstetrics and Gynecology Volume 6. No. 2 April 2018
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To investigate the HOXA11 gene profile on endometriosispatients with infertility in Indonesia.Methods: This cross sectional study was conducted in Dr. CiptoMangunkusumo Hospital from July 2015- June 2016. The subjectswere endometriosis patients with infertility who have beenconfirmed histopathological. The control group was taken fromnon-endometriosis and fertile patients. Eutopic endometriumsamples were taken and examined for the methylation of HOXA 11gene.Results: Both groups consist of six patients. The difference ofmethylation of HOXA 11 gene between those two groups isstatistically significant (p=0.03). There was hyper methylation inendometriosis group.Conclusion: There is a hyper methylation of HOXA 11 gene ineutopic endometrium of endometriosis patients with infertility.Thus, possibly can explain the poor endometrial receptivityin endometriosis patient and give a broad research area inepigenetic therapy of endometriosis.[Indones J Obstet Gynecol 2018; 6-2: 110-113]Keywords: endometriosis, epigenetic, HOXA 11, infertility,methylation

ANALISIS KEMURNIAN EKSTRAKSI ASAM RIBONUKLEAT PADA SAMPEL DARAH MENSTRUASI Ocktariyana Ocktariyana; Dian Lestari; Asmarinah Asmarinah
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 17 No 2 (2022): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : POLITEKNIK KESEHATAN KEMENKES PALEMBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v17i2.1392

Latar Belakang: Asam nukleat mengandung materi genetik dan berfungsi untuk mengatur perkembangan biologis seluruh bentuk kehidupan secara seluler. Asam nukleat yang paling umum adalah Asam deoksiribonukleat (ADN) dan Asam ribonukleat (ARN). Untuk mengeluarkan ARN dari dalam intisel maka diperlukan suatu teknik isolasi. Suatu ekstraksi asam nukleat dikatakan baik jika dari prosedur yang dilakukan bisa didapatkan asam nukleat yang murni dan utuh. Penelitian ini bertujuan untuk mengetahui bagaimana tingkat kemurnian ekstrak asam nukleat dari sampel darah menstruasi menggunakan teknik isolasi asam nukleat. Metode: Sampel darah menstruasi dikumpulkan dengan cara ditampung pada kertas saring yang di desain khusus. Sampel akan diekstraksi menggunakan Quick-ARN Miniprep Plus Kit R1058 Zymo Research untuk isolasi ARN, selanjutnya diukur tingkat kemurnian dengan menggunakan alat nanodrop berdasarkan prinsip spektrofotometri. Data diolah secara statistic dengan menggunakan analisis deskripsi dalam disajikan dalam bentu distribusi frekuensi dan nilai rerata. Hasil: pada penelitian ini diperoleh bahwa rerata tangka kemurnian ARN sampel darah menstruasi yang ditampung pada kertas saring pada Panjang gelombang A260 / A280 adalah 2,07, dan Panjang gelombang A260 / A230 adalah 2,1. Kesimpulan: Isolasi ARN pada sampel darah menstruasi yang ditampung di kertas saring memiliki tingkat kemurnian yang optimal.

Sperm DNA Fragmentation and Chromatin Maturation of Prepared Sperm with ALA Supplementation: The Effect of Embryo Development Quality silvia werdhy lestari; aucky hinting; Petrus Supardi; anom Bowolaksono; asmarinah asmarinah
Journal of Global Pharma Technology Volume 11 Issue 09: (2019) September 2019
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract: This study was aimed to determine the differences in embryo quality between spermatozoa with good or poor of DNA fragmentation index and chromatin maturation, through spermatozoa preparation with the addition of ALA. This experimental study compared the quality of embryos that fertilized by spermatozoa with DNA fragmentation and chromatin maturation, with and without ALA supplementation. In this study semen samples from 20 patients were collected, which were then prepared and examined for DNA fragmentation index (DF) and chromatin maturation (CM). Then the sample was divided into 4 groups, namely the good DF - good CM, good DF - poor CM, poor DF - good CM and poor CM - poor CM, further divided again with and without treatment (ALA supplementation). After injection of spermatozoa into the oocyte, the development of embryo was observed in each group. In the group without treatment, significant differences in embryo quality were obtained between groups of good DF - good CM with poor DF - good CM, good DF - good CM with poor DF - poor CM, good DF - poor CM with poor DF - good CM and good DF - poor CM with poor DF - poor CM (p <0.05). In the treatment group, there were not significant difference in the quality of the embryo between treatment groups, namely with or without ALA, in the group with good or poor DF, and with good or poor CM (p> 0.05). Embryo fertilized by spermatozoa with good or poor DF and with good or poor CM, with the supplementation of ALA, the quality was not significantly better than without the supplementation of ALA. Keywords: spermatozoa DNA fragmentation, spermatozoa chromatin maturation, ALA, in vitro fertilization, embryo quality

Mechanism of Immune System Dysfunction, Apoptosis and Oxidative Stress on Endometriosis Miftahuz Zakiyah; Asmarinah Asmarinah
Journal of Biomedicine and Translational Research Vol 9, No 2 (2023): August 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i2.16885

Endometriosis is gynecology disease that still needs advance knowledge to develop best treatment for enhancingÂ the best quality of patient life. It is characterized by forming the endometrium tissue outside the uterus and happened approximately in 5-10% of reproductive women. Endometriosis with poor prognosis can be a high risk of endometrium cancer, ovarian cancer and results in infertility. A deep attempt to reach the knowledge of clear molecular etiology of endometriosis is needed. Here, there are three molecular mechanism dysfunction that occurs on endometriosis; (1) immune system dysfunction, (2) disturbance in apoptosis signal, (3) and oxidative stress elevation. Therefore, the determination of molecular pathogenesis of endometriosis will be useful in developing of diagnostic and therapeutic methods.

ANALISIS KEMURNIAN EKSTRAKSI ASAM RIBONUKLEAT PADA SAMPEL DARAH MENSTRUASI Ocktariyana, Ocktariyana; Lestari, Dian; Asmarinah, Asmarinah
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 17 No 2 (2022): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v17i2.1392

Latar Belakang: Asam nukleat mengandung materi genetik dan berfungsi untuk mengatur perkembangan biologis seluruh bentuk kehidupan secara seluler. Asam nukleat yang paling umum adalah Asam deoksiribonukleat (ADN) dan Asam ribonukleat (ARN). Untuk mengeluarkan ARN dari dalam intisel maka diperlukan suatu teknik isolasi. Suatu ekstraksi asam nukleat dikatakan baik jika dari prosedur yang dilakukan bisa didapatkan asam nukleat yang murni dan utuh. Penelitian ini bertujuan untuk mengetahui bagaimana tingkat kemurnian ekstrak asam nukleat dari sampel darah menstruasi menggunakan teknik isolasi asam nukleat. Metode: Sampel darah menstruasi dikumpulkan dengan cara ditampung pada kertas saring yang di desain khusus. Sampel akan diekstraksi menggunakan Quick-ARN Miniprep Plus Kit R1058 Zymo Research untuk isolasi ARN, selanjutnya diukur tingkat kemurnian dengan menggunakan alat nanodrop berdasarkan prinsip spektrofotometri. Data diolah secara statistic dengan menggunakan analisis deskripsi dalam disajikan dalam bentu distribusi frekuensi dan nilai rerata. Hasil: pada penelitian ini diperoleh bahwa rerata tangka kemurnian ARN sampel darah menstruasi yang ditampung pada kertas saring pada Panjang gelombang A260 / A280 adalah 2,07, dan Panjang gelombang A260 / A230 adalah 2,1. Kesimpulan: Isolasi ARN pada sampel darah menstruasi yang ditampung di kertas saring memiliki tingkat kemurnian yang optimal.

Stone recurrence among Indonesian kidney stone formers: a comprehensive analysis of genetic polymorphism, demographic, and clinical factors Atmoko, Widi; Savira, Missy; Fajriani, Rosalina; Wistara, Sevita Sathya; Asmarinah; Harahap, Alida Roswita; Witjaksono, Fiastuti; Werdhani, Retno Asti; Safriadi, Ferry; Hamid, Agus Rizal Ardy Hariandy; Birowo, Ponco; Rasyid, Nur; Taher, Akmal
Medical Journal of Indonesia Vol. 33 No. 3 (2024): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247596

BACKGROUND The recurrence rate of kidney stone disease (KSD) can exceed 50% within 5 years. This study aimed to investigate factors associated with stone recurrence based on single nucleotide polymorphisms (SNPs) of the calcium-sensing receptor (CaSR) rs1801725 and rs1042636, demographic, and clinical profiles. METHODS We collected data on the demographic and clinical characteristics of 80 adult kidney stone formers from April 2021 to September 2022, including peripheral blood samples, morning and 24-hour urine, and 7-day water intake records. Real-time polymerase chain reaction was used to evaluate SNP. Comparisons were made between patients with recurrent and first-time stones. RESULTS 41% of 80 patients have experienced stone recurrence. Recurrent stone formers exhibited significantly higher 24-hour urine calcium excretion (p = 0.03) and lower serum calcium levels (p = 0.019) than first-time stone formers. Hypocitraturia (100%), low urine volume (78%), and hyperoxaluria (55%) were the main abnormalities of all patients. No significant differences were found in CaSR gene polymorphisms and other demographic, biochemical, or clinical parameters. CONCLUSIONS Recurrent stone formers had higher 24-hour urine calcium excretion and lower serum calcium levels. Other risk factors and CaSR polymorphisms may insignificantly affect KSD recurrence.

Body Composition Parameters, Adiponectin, Leptin and Adiponectin/Leptin Ratio are Correlated with LH/FSH Ratio in Women with PCOS but not in Women without PCOS Gita Pratama; Wiweko, Budi; Asmarinah; Widyahening, Indah Suci; Andraini, Trinovita; Hartanto Bayuaji; Andon Hestiantoro
Indonesian Journal of Obstetrics and Gynecology Volume 12 No. 1 January 2024
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v12i1.2031

Objective: To investigate the correlation between body composition parameters, adiponectin, leptin and the adiponectin/leptin ratio and the LH/FSH ratio in women with polycystic ovary syndrome (PCOS). Methods: A cross-sectional study was conducted at Reproductive Cluster Yasmin, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, with sixty women with PCOS and sixty healthy women as controls (matched for age and BMI). Body composition parameters, including body weight, body mass index (BMI), waist circumference (WC), waist to hip ratio (WHR), percent body fat (PBF), visceral fat area (VFA), percent subcutaneous fat (PSF) and skeletal muscle mass (SMM), were measured; levels of fasting glucose, fasting insulin, testosterone, and sex hormone binding globulin (SHBG) were measured; and homeostatic model assessment for insulin resistance (HOMA-IR) values, anti-Mullerian hormone (AMH), free androgen index (FAI), Ferriman-Gallwey (FG) score, adiponectin levels, leptin levels, adiponectin/leptin ratio, LH, FSH and LH/FSH ratio were measured. Results: Body composition parameters (body weight, BMI, WC, WHR, PBF, VFA, PSF, SMM) were not significantly different between women with PCOS and controls. Fasting insulin (P<0.05), HOMA-IR (P<0.05), AMH (P<0.01), FAI (P<0.01), FG score (P<0.01) and LH/FSH ratio (P<0.05) were higher in PCOS women. Adiponectin (P<0.01) was lower in PCOS women, while leptin and the adiponectin/leptin ratio were not significantly different between groups. Most of body composition parameters, adiponectin, leptin and adiponectin/leptin ratio were correlated with HOMA-IR in both groups. SMM was positively correlated with the LH/FSH ratio, while body weight, BMI, WC, PBF, VFA, and PSF were inversely correlated with the LH/FSH ratio in PCOS patients but not in controls. WHR was not correlated in either group. Leptin (r=-0.278; P<0.05) was negatively correlated with the LH/FSH ratio only in the PCOS group. Adiponectin (r=0.394; P<0.01) and the adiponectin/leptin ratio (r=0.413; P<0.01) were also positively correlated with the LH/FSH ratio only in the PCOS group. AMH was correlated with the LH/FSH ratio, whereas testosterone level, FAI, FG score, fasting insulin level and HOMA-IR value were not correlated with the LH/FSH ratio in PCOS women. Conclusion: Most of the body composition parameters, leptin, adiponectin and the adiponectin/leptin ratio were significantly correlated with HOMA-IR in both groups. However, correlations of those parameters with LH/FSH ratio were found only in PCOS but not in women without PCOS. Adiponectin and leptin may play a significant role in the mechanism of neuroendocrine disorders in PCOS, which is characterized by an increased LH/FSH ratio. Keywords: adiponectin, adiponectin/leptin ratio, body composition, HOMA-IR, leptin, LH/FSH ratio, PCOS

mRNA Expression and DNA Methylation of CXCL16 in Menstrual Blood and Endometrium Tissue of Subjects with Endometriosis and Pelvic Pain Febriyeni, Febriyeni; Hestiantoro, Andon; Tulandi, Togas; Muharam, Muharam; Asmarinah, Asmarinah; Sandra, Ferry
The Indonesian Biomedical Journal Vol 16, No 2 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i2.2958

BACKGROUND: The cytokine chemokine ligand 16 (CXCL16) plays an important role in the pathophysiology of endometriosis by regulating the inflammatory response and contributing to the pain-associated endometriosis. Despite this, the impact of epigenetic modifications, such as DNA methylation, on CXCL16 has yet to be fully understood. Therefore, this research was conducted to assess both the mRNA expression and DNA methylation levels of the proinflammatory gene CXCL16 in the endometrium tissue and menstrual blood of patients with and without endometriosis.METHODS: Thirty-five women with and without endometriosis were involved in this research. Subjects' menstrual blood samples were collected using filter paper pads, meanwhile the endometrium tissue were collected by performing biopsy, from which DNA and RNA were extracted. The DNA methylation levels of the CXCL16 were measured using the pyrosequencing method following bisulfite conversion treatment. Meanwhile, the mRNA expression level was measured using the quantitative polymerase chain reaction (qPCR) method and analyzed with the Livak method.RESULTS: The mRNA expression of CXCL16 in menstrual blood of endometriosis subjects was 2.42 times higher compared to control group (p=0.030). Furthermore, the expression of CXCL16 in menstrual blood was identical to that in endometrial tissue (p=0.173). DNA methylation analysis showed that CXCL16 in the menstrual blood of endometriosis subjctes had lower methylation levels compared to controls (p=0.004), indicating hypomethylation.CONCLUSION: Increased mRNA expression and hypomethylation of CXCL16 in the menstrual blood of endometriosis patients could serve as a direct marker for diagnosing endometriosis. However, further study to validate these findings and explore the potential of CXCL16 as a diagnostic tool, and additional research involving larger patient for the cohorts study is necessary.KEYWORDS: CXCL16, DNA methylation, endometrium, menstrual blood, mRNA expression, pain

SOX2 expression in the primary tumor of castration-naive metastatic prostate adenocarcinoma in association with metastasis extent Saraswati, Meilania; Kekalih, Aria; Lisnawati; Rahadiani, Nur; Asmarinah; Hernowo, Bethy Suryawathy; Hamid, Agus Rizal Ardy Hariandy; Mochtar, Chaidir Arif
Medical Journal of Indonesia Vol. 35 No. 1 (2026): March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247588

BACKGROUND Poor prognosis in patients with metastatic prostate adenocarcinoma (mPCa) may be due to the expression of stem cell-related genes. This study aimed to demonstrate the association between the expression of cancer stem cell markers and metastasis in patients with castration-naive mPCa. METHODS This cross-sectional, analytical study investigated a formalin-fixed paraffin-embedded prostate specimens from patients diagnosed in Cipto Mangunkusumo Hospital. Patients aged ≥50 years old were grouped based on the extent of metastases (high-volume disease [HVD] and low-volume disease [LVD]). In each case, immunohistochemical staining for CD133, CD44, SOX2, and androgen receptor was performed and analyzed using H-score. All data were recorded and analyzed using SPSS software version 20.0. RESULTS A total of 61 patients were recruited from 2020 to 2023 and divided into the HVD (n = 38) and LVD (n = 23) groups, with a mean age of 67.9 years. 45 of the patients had International Society of Urological Pathology (ISUP) grade 5 disease, while 16 of them had grade <5. A significant difference of ISUP grade and PSA serum level was observed in the HVD versus LVD group (p = 0.017 and <0.001, respectively). Additionally, a significant association was found between SOX2 expression and metastatic extent. CONCLUSIONS The LVD group showed higher SOX2 expression in the primary tumor compared to the HVD group. Different SOX2 expressions in various sites and stages may be due to the cancer cells’ systemic network.

Comparison of DNA Extraction Feasibility from Menstrual Blood and Endometrial Tissue in Reproductive-Aged Women Febriyeni; Hestiantoro, Andon; Asmarinah; Tulandi, Togas; Hasnita, Evi; Zuraida; Harnaldo Putra, Billy; Octariyana; Rizki Amanda, Clara; Astria Rishel, RIka
Jurnal Kesehatan - STIKes Prima Nusantara Vol 16 No 2 (2025): Jurnal Kesehatan Volume 16 Nomor 2 Tahun 2025
Publisher : LPPM Universitas Prima Nusantara Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35730/jk.v16i2.1314

ABSTRACT The endometrium is a highly vascularized tissue composed of numerous blood vessels, with cells that are formed and destroyed during each menstrual cycle. Menstrual blood, which is shed cyclically from the endometrium in women of reproductive age, contains endometrial tissue or cells. Both deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are the primary nucleic acids present in cells with nuclei. In this study, 20 samples of menstrual blood and endometrial tissue were collected from women of reproductive age. Menstrual blood was gathered using specially designed filter paper, and DNA was extracted using the QIAamp DNA Mini Kit (Qiagen, Cat No.: 51304). Endometrial tissue was obtained via biopsy, and DNA was extracted using the Geneaid DNA Isolation Kit (Geneaid, New Taipei, Taiwan). DNA purity and concentration were measured using a Thermo Scientific Nano-Drop microvolume Spectrophotometer. The results indicated an average DNA purity of 1.88 ± 0.09 and a mean concentration of 116.9 ng/µL for menstrual blood, while endometrial tissue had a median DNA purity of 1.92 and a concentration of 192 ng/µL. Statistical analysis revealed no significant difference in DNA purity between menstrual blood and endometrial tissue (p = 0.083), but a significant difference in DNA concentration was observed (p = 0.002). Although there was a slight difference in DNA concentration, both menstrual blood and endometrial tissue samples were equally effective in preserving optimal purity and concentration of DNA molecules. Keywords: Deoxyribonucleic Acid, DNA, Endometrial Tissue, Menstrual Blood, Nucleic Acid.

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