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All Journal Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Partner Animal Production : Indonesian Journal of Animal Production Livestock and Animal Research Jurnal Produksi Ternak Terapan (JPTT) Jurnal Kedokteran Hewan Farmers: Journal of Community Services Jurnal Agripet Animal Production
Siti Darodjah Rasad
Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Estrous Performance of Etawah Crossbred Goats Following Different Estrous Synchronization Methods Solihati, Nurcholidah; Rasad, Siti Darodjah; Winangun, Kikin; Toha, Toha
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.74

Abstract

The objective of the research was to determine the estrous profile of Etawah Crossbred goats after estrous synchronization with different methods. Eighteen does aged 12-24 month old were divided in three groups to receive estrous synchronization treatments (T1 = 14 days intravaginal implant of 60 mg of progesterone (MPA), T2 = two times injection of 5 mg PGF2α (lutalyse) in 11 days interval, and T3 = 10 days of intravaginal implant of 60 mg of progesterone (MPA) + injection of 5 mg PGF2α 48 hours before removal) with six replications. The parameters consisted of estrous behaviour, changes in size and colour of vulva, and duration of estrus when the number of superficial and keratin cells were dominating in the vaginal mucus cell. Data from estrous behaviour and score of vulvar colour was analyzed using Kurkal Wallis test, while onset of estrus, size of vulva slit and estrous duration was analyzed using ANOVA and Duncan test. The result showed that estrous behaviour and changes in color and size of vulva were not significantly different, but estrous duration was significantly different. Estrous duration in T1 (31.30 hour) and T2 (31.10 hour) was significantly longer than that of T3 (11.36 hour). It is concluded that different methods of estrus synchronization affected estrous quality equally but it affected the estrous duration differently based on vaginal mucus cells. Treatment implant vaginal sponge content progesterone for 14 days and double injection of PGF2α with 11-day interval given longest estrous duration.
X-Y Chromosom Bearing Sperm Proportion of Local Ram after Sexing with Different Combination of Bovine Serum Albumin (BSA) Concentration Solihati, Nurcholidah; Rasad, Siti Darodjah; Hilmia, Nena; Winangun, Kikin; Toha, Toha
ANIMAL PRODUCTION Vol. 25 No. 1 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.1.137

Abstract

The research aims to evaluate proportion of X-Y chromosom bearing sperm after sexing of local ram semen with different combination of BSA concentration. The research object was ten ejaculated semen of local ram, three years old. The research design used CRD (completely randomize design) with four treatments of BSA concentration combination on upper and bottom layer (T1: 3% & 6%, T2: 4% & 6%, T3: 5% & 10%, and T4: 6% & 12%), and 10 repetitions. Data analysis using analysis variance and Duncan’s Multiple Range Test. The result showed that combination of BSA concentration was significantly effect on proportion of X-Y chromosome bearing sperm of local ram sperm. The higher average proportion of X- and Y- chromosome bearing sperm was obtained at combination 5% (75.55±1.09% for X) at upper layer and 10% BSA (76.45±1.12% for Y) of bottom layer. The conclusion is that combination of BSA concentration significantly effect on proportion of X-Y chromosome bearing sperm, and concentration of 5% and 10% BSA at upper layer and bottom layer gave the higher proportion of X-Y chromosome bearing sperm of local ram sperm.
Effect of PMSG administration in combination with vaginal sponge on estrous occurrence and litter size of Javanese sheep Setiawan, Rangga; Rasad, Siti Darodjah; Solihati, Nurcholidah; Widyastuti, Rini; Soeparna, Soeparna
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.4349

Abstract

AbstractThe use of vaginal sponge in estrous synchronization has been a popular method to uniform estrous cycle of small ruminants. In order to increase the litter size, the use of the sponge is combined with PMSG. Therefore the objective of this study was to evaluate the effect of PMSG in combination with vaginal sponge on estrous response and litter size of Javanese local sheep. The study was conducted in Faculty of Animal Husbandry, Padjadjaran University, Indonesia with 32 non-pregnant local sheep. All these sheep was divided into four groups of treatment, i.e: 1) group of sheep was subjected to vaginal sponge (VS) treatment containing 30 mg Medroxyprogesterone acetat (MAP) inserted in the vagina for 14 days; 2) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection -1 d before VS removal; 3) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection at the day of VS removal; 4) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection +1 d after VS removal. The result showed that all treatment groups had 100% of estrous response. However, the occurrence of estrous time varied among group after VS removal. Group 1 (G1) and G4 tend to have estrous occurrence at day 3 after VS removal by 55% and 75% response, respectively. Whilst, G2 and G3 tend to have estrous occurrence respectively at day 1 (62,5%) and day 2 (50%) after VS removal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have a 88,9% and 87,5%, respectively. No significant effects of the treatments on the ewes litter size. In conclusion, the use of PMSG administration 24 h prior, after, or at sponge withdrawal increase a tighter synchrony of oestrous and pregnancy rate, but has no effect on the increasing of litter size in Javanese local sheep.Keywords: Vaginal sponge, PMSG, estrous response, pregnancy rate, litter size, sheep
Effect of Skim Nanoparticles on the Motility and Kinematics of Simmental Cattle Frozen Semen Anggraeni, Fitri Dian; Rasad, Siti Darodjah; Solihati, Nurcholidah
Jurnal Agripet Vol 24, No 2 (2024): Volume 24, No. 2, October 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i2.31736

Abstract

Nanotechnology has positively impacted the development of extenders used in the cryopreservation of spermatozoa in livestock. The inclusion of nano skim in the extender is expected to preserve semen quality after thawing. This study aimed to determine the optimal concentration of nano skim. Various concentrations of nano skim (0%, 1.66%, 3.33%, 5%, 6.66%, 8.33%, and 10%) with the addition of 10% nano egg yolk in each extender were compared. Fresh semen samples were collected from three three-year-old Simmental bulls using an artificial vagina. The study parameters included motility (total motility, progressive motility) and kinematics (velocity curve linear (VCL), velocity straight linear (VSL), velocity average pathway (VAP), amplitude lateral head (ALH), beat cross frequency (BCF), straightness (STR), linearity (LIN), and wobble (WOB). A completely randomized design was employed, and data were analyzed using analysis of variance (ANOVA). Fresh semen dilution results indicated that only the control group (0%) and the nano skim group with concentrations of 6.66% and 8.33% were eligible for further processing into frozen semen. The results showed no significant difference between the three treatment groups on sperm motility and kinematics of Simmental cattle after thawing (p0.05). The control group exhibited the highest percentage of total motility, VCL, VAP, and ALH values, while the 6.66% nano skim group outperformed the 8.33% nano skim group. The highest percentage of progressive motility and VSL value was observed in the 6.66% nano-skim group compared to the 8.33% nano-skim and control groups. The highest values of BCF, WOB, LIN, and STR was observed in the nano-skim group at 8.33% compared to the nano-skim group at 6.66% and control. The study concluded that 6.66% nano skim was the optimal concentration to maintain the quality of frozen semen of Simmental cattle.
Pelatihan Penerapan Sinkronisasi Estrus dan Inseminasi Buatan untuk Peningkatan Produktivitas Budidaya Domba di Desa Pamulihan Kecamatan Pamulihan Kabupaten Sumedang Solihati, Nurcholidah; Rasad, Siti Darodjah; Setiawan, Rangga; Widyastuti, Rini; Sari, Aprilianna P.Z.N.L.; Toha, Toha; Winangun, Kikin
Farmers : Journal of Community Services Vol 7, No 1 (2026): Farmers: Journal of Community Services
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v7i1.69538

Abstract

Sinkronisasi estrus dan inseminasi buatan (IB) merupakan bioteknologi reproduksi yang dapat membantu manajemen reproduksi di suatu kawasan peternakan dimana peternak dapat mengatur waktu perkawinan yang dapat disesuaikan dengan permintaan pasar. Desa pamulihan Kecamatan Pamulihan Kabupaten Sumedang, memiliki beberapa peternak domba Garut yang memiliki usaha secara komersil dengan jangkauan pemasaran yang cukup luas. Tujuan kegiatan ini yaitu memberikan pengetahuan kepada peternak domba tentang sinkronisasi estrus dan IB untuk dapat diaplikasikan pada sistem pemeliharaan domba yang mereka miliki. Metode yang dilakukan meliputi penyuluhan, diskusi dan tanya jawab, pelatihan dan demonstrasi. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan peternak terhadap materi pelatihan terkait sinkronisasi estrus dan IB yang diketahui dari hasil pre-test dan post-tes, yaitu sebesar 53%. Hasil ini menunjukkan bahwa metode yang dilakukan berupa penyuluhan dan pelatihan cukup efektif memberikan pengetahuan kepada peternak domba di Desa Pamulihan. Disimpulkan bahwa peserta pelatihan mampu menerima ilmu dan keterampilan yang telah diberikan, peserta juga tertarik untuk dapat mengaplikasikan hasil pelatihan terhadap domba yang mereka miliki, namun untuk pelaksanaan secara mandiri masih memerlukan pembinaan dan pendampingan
The Effect of Incubation Time on the Quality of Post-Thawed Ram Sexed Sperm Solihati, Nurcholidah; Rasad, Siti Darodjah; Setiawan, Rangga
Livestock and Animal Research Vol 22, No 2 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i2.90802

Abstract

Objective: The sperm sexing using an albumin column requires an incubation process at a certain temperature for sperm to move through the albumin layer. It is thought that the incubation time can influence the quality of the semen resulting from post-thawing sexing. The aim of this research was to determine the effect of incubation time on the quality of sexed sperm post-thawing and to find out the optimum incubation time that provide the best sperm quality.Methods: This research was carried out in a completely randomized design, using three incubation times (T1= 45 minutes, T2= 60 minutes, T3= 75 minutes) with six replications. The parameters consisted of motility, abnormality and acrosome integrity of sexed sperm in the upper and bottom fractions. Data were analyzed using analysis of variance (Anova) and continued with the Duncan test.Results: The results showed that the incubation time had a significant effect on motility, intact acrosome cap and recovery rate, but had no significant effect on abnormality of post thawed sexed sperm. For both the upper and bottom fraction, incubation times of 45 and 60 minutes produced the best motility and recovery rate compared to 75 minutes, but the best intact acrosome cup was obtained from an incubation time of 45 minutes.Conclusions: Based on the research results, it can be concluded that the length of incubation time influences the quality of post thawed ram sexed sperm, and 45 minutes is the optimal incubation time to provide the best quality.
Co-Authors . Paggi Anggraeni, Fitri Dian Annisa Yusrina Annisaa Yusrina Bambang Hadisutanto Bambang Purwantara Fahmy Avicenna HARSA, DZAKY FATIH Ilham Nugraha Iwan Setiawan K Winangun Kikin Winangun N Solihati Nena Hilmia Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Rangga Setiawan Rangga Setiawan Rini Widyastuti Sari, Aprilianna P.Z.N.L. Soeparna Soeparna Soeparna Soeparna, Soeparna Sutarman Muhardja Toha, Toha Winangun, Kikin