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Isolasi dan Identifasi Mikroba Rumen Penghasil Antihistamin “Histamine Methyl Transferase“ Ning Iriyanti; Budi Rustomo; Efka Aris Rimbawanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 1 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.1.88

Abstract

The aim of this research was to find antihistamine ”Histamine Methyl Transferase” of microbe producers through isolation and testing of their activities on various substrates. The isolation process found four putative isolates i.e. N2k, N2b, N3 and N5. Their general characteristics were round, big and formed regular colonies; regular edge and wavy colonies. Colonies were whitish, shiny and had low convex elevation. N2k, N2b, and N5 isolates were Gram negative bacteria whereas N3 was Gram positive. Those isolates were non-motile. Further tests were conducted to examine the isolate capability to produce catalase and utilize citric acid as the carbon source to which they showed positive result. Nitrate reduction test showed positive results for N2b and N5 but not forN2k and N3. The ability to ferment sugars into acid and gas indicated that all isolates except N3 were capable of fermenting glucose and lactose. However, only N2k was capable to produce gas.
Tingkah Laku Makan dan Kecernaan Nutrien Berbagai Bangsa Sapi Lokal yang Diberi Pakan Jerami Padi dan Konsentrat Apit Mulyana; Muhamad Bata; Efka Aris Rimbawanto
Jurnal Agripet Vol 22, No 1 (2022): Volume 22, No. 1, April 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i1.20490

Abstract

ABSTRACT. Penelitian ini bertujuan mengetahui pengaruh bangsa sapi yang berbeda terhadap tingkah laku makan dan kecernaan nutrien pada pemberian pakan jerami padi dan konsentrat. Penelitian dilaksanakan selama empat (4) bulan di UD. Sapi Amanah, Desa Karanggintung, Kecamatan Sumbang, Kabupaten Banyumas. Materi yang digunakan adalah 40 ekor sapi lokal jantan dari bangsa sapi Madura (M), Sumba Ongole (SO), Bali (B), dan Bali Timor (BT) masing-masing 10 ekor. Rerata bobot hidup awal masing-masing bangsa sapi berturut-turut adalah 236,904,527 kg, 283,508,873 kg, 224,404,814 kg, dan 282,107,802 kg untuk M, SO, B, dan BT. Sapi tersebut diberi pakan konsentrat 2,5% (BK=86,53%) dari bobot hidup dan jerami padi disediakan secara ad libitum. Penelitian dirancang menurut rancangan acak lengkap dengan empat (4) bangsa sapi sebagai perlakuan. Peubah yang diamati adalah tingkah laku makan dan kecernaan bahan kering (KcBK) dan bahan organik (KcBO). Hasil penelitian menunjukkan bahwa bangsa sapi berpengaruh nyata (P0,05) terhadap kecepatan makan sore hari; lama waktu makan siang, malam dan sehari penuh, KcBK dan KcBO. Akan tetapi, tidak berpengaruh nyata (P0,05) terhadap kecepatan makan pagi hari; frekuensi makan dan ruminasi siang, malam dan sehari penuh; lama waktu ruminasi siang, malam dan sehari penuh. Kesimpulannya adalah terdapat persamaan dan perbedaan tingkah laku makan, kecernaan bahan kering dan bahan organik pada bangsa sapi lokal dan kecernaan BK dan BO pada sapi SO lebih rendah dibandingkan dengan sapi lainnya.(Feeding behavior and nutrient digestibility of various local cattle breeds fed with rice straw and concentrates)ABSTRAK. This study aimed to determine the effect of different breeds of local cattle on feeding behavior and nutrient digestibility of rice straw and concentrate diet. The research was conducted for four (4) months at UD. Sapi Amanah, Karanggintung Village, Sumbang District, Banyumas Regency. The materials used were 40 local bulls from Madura (M), Sumba Ongole (SO), Bali (B), and Bali Timor (BT) and each of the breeds was ten (10) cattle. The initial body weights of each breed were 236,904,527 kg, 283,508,873 kg, 224,404,814 kg and 282,107,802 kg for M, SO, B and BT, respectively. They were fed with 2.5% concentrate (dry matter/DM=86.53%) from body weight and rice straw which was provided ad libitum. The study was designed according to Randomized Complete Design with four (4) local cattle breeds as treatments. Variables measured were feeding behavior and digestibility of DM and organic matter (OM). The results showed that cattle breed had a significant effect (P0.05) on eating rate in the afternoon, duration of daytime, nighttime and one-day meals, DM and OM digestibility. However, there was no significant effect (P0.05) on eating rate in the morning; frequency of daytime, nighttime, and one-day meals and rumination; spent time of daytime, nighttime, and one-day rumination. In conclusion, there are similarities and differences in feeding behavior, DM and OM digestibility among local cattle breeds, and digestibility of dry matter and organic matter of SO was lower than other cattles.
Produk Metabolisme Rumen pada Sapi Perah Laktasi S.N.O. Suwandyastuti; Efka Aris Rimbawanto
Jurnal Agripet Vol 15, No 1 (2015): Volume 15, N0. 1, April 2015
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v15i1.2284

Abstract

(Rumen metabolism product on lactating dairy cattle)ABSTRACT. The rumen microorganism, as yeast, have an important role in rumen fermentation processes and the rumen metabolism product. A research had been done to study the use of yeast, Saccharomyces cereviseae in Lactating dairy cattle ration. The research had been conducted by experimental method, in a Latin Square Design. The animal were subjected as column and periods function as row. The treatment to be tested were four levels of yeast addition, namely : 0, 5, 10 and 15 g/cattle/day. The variables measured were rumen metabolism product : Total Volatile Fatty Acids (T-VFA), Acetate (C2), Propionate (C3), Butyrate (C4), Formiate, Valerate, Nitrogen Ammonia and C2/C3. Based on the all variables measured, it was indicated that the addition of yeast Saccharomyces cereviseae up to 15 g/cattle/day have not changed the rumen metabolism product on lactating dairy cattle; although it was a normally production of total VFA (96,86 9,94 mM/L and C2/C3 (3,08 0,14), but it was very high production of N-NH3 (12,85 2,72 mM/L). To increase the efficiency of metabolism processes, it is need the addition of fermentable carbohydrate in ration.
Pengaruh Imbangan Jerami Padi, Dedak Padi dan Onggok Terfermentasi terhadap Kecernaan dan Produk Fermentasi Rumen Secara In Vitro S.N.O. Suwandyastuti; Efka Aris Rimbawanto; Ning Iriyanti
Jurnal Agripet Vol 10, No 2 (2010): Volume 10, No. 2, Oktober 2010
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v10i2.646

Abstract

The influence of the ratio of fermented rice straw, rice bran and cassava solid waste upon the In vitro digestibility and rumen fermentation productABSTRACT. Chemical and physical treatments have been attempted to improve the utilization of agro industrial wastes, but the result is not efficient and caused pollution. Besides, biological treatments using the microbes have been used to improve the nutritive value and utilization of agro industrial wastes. The current experiment was conducted to find out the optimal ratio of fermented rice straw, fermented rice bran and fermented tapioca waste. There were five kinds of ratio of fermented rice straw, fermented rice bran and fermented tapioca waste, namely: K1 (70% fermented rice straw + 15% fermented rice bran + fermented tapioca waste), K2 (60% fermented rice straw + 20% fermented rice bran + 20% fermented tapioca waste), K3 (50% fermented rice straw + 25% fermented rice bran + 25% fermented tapioca waste), K4 (40% fermented rice straw + 30% fermented rice bran + 30% fermented tapioca waste) and K5 (30% fermented rice straw + 35% fermented rice bran + 35% fermented tapioca waste) An in vitro technique, using completely Randomize Block Design was applied and each treatment was repeated four times. Variables measured were Dry matter and Organic matter digestibility and rumen fermentation products (volatile fatty acid and N-NH3 concentration). The dry matter digestibility of K1, K2, K3, k4 and K5 was 29.39, 31.27, 32.33, 33.71 and 34.82%, respectively. The organic matter digestibility of K1, K2, K3, K4 and 5 was 30.82, 31.27, 32.73, 34.94, and 34.92, respectively. Volatile fatty acid concentrations of K1, K2, K3, K4 and K5 were 95.19, 91.77, 87.21, 104.31, 106.59 mM/l, respectively. N-NH3 concentrations of K1, K2, K3, K4 and K5 were 0.97, 0.93, 0.93, 1.00, 1.04 mM/l, respectively. Significant difference (P0.01) was only found in dry matter digestibility among treatments, while others variables were not significantly different among treatments. It was indicated that the optimal ratio was K4.
Pemanfaatan Limbah Berserat Dalam Konsentrat Untuk Sapi Jantan Umur Satu Tahun S.N.O. Suwandyastuti; Efka Aris Rimbawanto
Jurnal Agripet Vol 11, No 1 (2011): Volume 11, No. 1, April 2011
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v11i1.390

Abstract

The utilization of fibrous waste in concentrate for yearling male cattleABSTRACT. A series of experiment had been conducted to improved the quality of rice straw, rice bran and cassava solid waste through biological pretreatment using several species of microbes. Based on the digestibility and the fermentation product, indicated that 30 percent of rice straw, 35 percent of rice bran and 35 percent of cassava solid waste was the optimal ratio for concentrate formulated. The present experiment was carried out by experimental method with Completely Randomized Design, on twelve yearling male cattle of Ongole grade. The treatment tested were 3 physical form of the concentrate : (1) unfermented mesh; (2) fermented mesh and (3) fermented pellet. The variables measured were : energy and protein digestibility, rumen fermentation product, nitrogen balance and daily body weight gain. The digestion and balance trial were carried out by the Total Collection Method. The analysis of covariance shown, that there were significantly effects of the treatment tested whether upon the protein and energy digestibility, the nitrogen balance (P0.01), nitrogen ammonia production and daily body weight gain (P0.05), but there was no significantly effect on the Total Volatile Fatty Acid and the proportion of the individual VFA (P0.05). Based on the all variables measured, it was indicated that fermented pellet was the best concentrate (composed by 30% of fermented rice straw, 35% of fermented rice brand and 35% of fermented cassava solid waste).
Biotransformasi Limbah Ikan Menjadi Bahan Pakan untuk Ruminansia Efka Aris Rimbawanto; S.N.O. Suwandyastuti; Sri Rahayu
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i1.287

Abstract

Biotransformation of fish waste as feed matter for ruminantABSTRACT. Fish waste (head, viscera and tails) which ensilage with saccharomyces sp and lactobacillus sp, had a high quality and protein content of about 53,18 0,83% and a good aroma. An experiment have been conducted by experimental method with in sacco trial and in vivo trial. The treatment tested were 4 kind of protein source : (1) fish waste silages mixed with tapioca ; (2) fish waste silages mixed with rice bran ; (3) soy bean kernels and (4) fish meal. The in sacco trial was carried out on 3 fistulated cows, to study the rate of protein degradability in the rumen. The in vivo trial was carried out by Completely Randomized Design on 16 heads of fat tail rams. The variable measured were: rumen fermentation product (VFA and N-NH3); energy and protein digestibility; daily body weight gain of the rams. The experiment showed, that the source of protein use have no significantly effect upon VFA both on the VFA total and individual VFA (acetate, propionate and butyrate). The highest propionate reach by soya bean kernels (P 0.01). Based on the all variables measured, it was indicated the silage of fish waste can be used to substituted of soya bean kernels or fish meal in the ration of sheep.
Penggunaan Onggok sebagai Sumber Energi dalam Ransum Sapi S.N.O. Suwandyastuti; Efka Aris Rimbawanto
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i1.262

Abstract

The utilization of cassava meal residues as energy source in dairy cows rationABSTRACT. The dairy farmer in the rural village, usually use rice brain and coconut meat kernels as the energy source, although coconut meat kernels is less palatable and too expensive. It is, therefore, important to seek for the other materials which are locally available and inexpensive. An experiment had carried out, using five lactating dairy cows in a Change Over Design as long as five periods. Each period lasted for 21 days, consisted of 14 days preliminary and seven days observation period. The animal function as column and the period as row, so that its 5 x 5 Latin Square. The treatment tested are five substitution levels of coconut meat kernels by cassava meal residues of 5, 7.5, 10, 12.5 and 15 percent dry matter ration respectively. The variables measured were : (1) ration digestibility and essential minerals balance; (2) daily milk production and milk composition; (3) rumen fermentation product. Based on the analysis of variance of all variables measured the experiment suggested that cassava meal residues can be used as the energy source to substitute coconut meat kernels in kernels in lactating dairy cattle ration of 5 up to 15 percent dry matter ration, without causing physiological disorder moreover, increases the milk composition.
Peningkatan Mutu Jerami Padi, Dedak Padi dan Onggok dengan Fermentasi Fungi dan Yeast S.N.O. Suwandyastuti; Efka Aris Rimbawanto; Prayitno Prayitno
Jurnal Agripet Vol 12, No 2 (2012): Volume 12, No. 2, Oktober 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v12i2.199

Abstract

The quality improvement of rice straw, rice bran and cassava waste by fermentation of fungi and yeast ABSTRACT. Biomass of agricultural residues are highly potential as ruminant feedstuff. However, it is characterized by high content of indigestible fiber and low nutritive value, due to the strong hydrogen bonds in the lignocelluloses. Biological treatment by using microbes seems to be an alternative, because of the capability, with no pollution problem. An experiment has been conducted to seek for the fungi and yeast which capable to improve the quality of rice straw, rice bran and cassava waste. The trial was done by the technique of in sacco and in vitro, in a completely randomized block design. The variables measure were : dry matter and protein digestibility, protein solubility and nutrient composition of the fermentation product. Based on the all variables measure, the current study concluded that the microbes chosen were : monoculture of T. viride for rice straw, monoculture of A. niger for rice bran and biculture of A luchuensis and S. cereviseae for cassava waste.
KONSENTRASI VFA DAN NH3 CAIRAN RUMEN DOMBA YANG DIBERI TEPUNG DAUN KELOR DAN MINYAK SAWIT SECARA IN VITRO Safitri, Afifah Dian; Suhartati, F M; Rimbawanto, Efka Aris
ANGON: Journal of Animal Science and Technology Vol 3 No 2 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1072.894 KB) | DOI: 10.20884/1.angon.2021.3.2.p149-155

Abstract

Background. The research entitled VFA and NH3 Concentrations of Rumen Fluid Given In Vitro Moringa oleifera Leaf Meal and Palm Oil, which was conducted on 27 June 2020 to 12 September 2020. Materials and Methods. The material used in the study was the rumen fluid of 3 sheep obtained shortly after slughter, and beforehand lamb have been fed with the same treatment as the study. The treatment rations used consisted of R0 = control feed without moringa leaves and palm oil, R1 = control feed + 2% moringa oleifera leaf meal from feed dry matter (DM), and R2 = control feed + 2% moringa leaf meal from feed DM + 0.5% palm oil from feed DM. The method in the study was carried out in vitro according to Tilley and Terry (1963) which was modified by Sutardi (1979), the variable measured was the VFA concentration using the steam distillation method according to Krooman et al. (1967), while the rumen fluid NH3 concentration was measured using the Conway (1940) microdifusion technique. Results. The results of the analysis of variance showed that moringa leaf meal and palm oil supplementation had no significant effect (P> 0.05) on the VFA and NH3 concentrations of sheep rumen fluid in vitro, but the data obtained by supplementing Moringa leaf meal and palm oil could increase VFA 0,95% and NH3 0,94%. Conclusion. It was concluded that the use of 2% Moringa leaf powder supplementation and 0,5% palm oil increased the VFA concentration by 0,95% and NH3 0,94% of lamb rumen fluid in vitro. nd NH3 0,94% of lamb rumen fluid in vitro.
SUPLEMENTASI RDP (RUMEN DEGRADABLE PROTEIN) DAN UDP (UNDEGRADABLE PROTEIN) DALAM RANSUM DOMBA TERHADAP DEGRADASI BAHAN KERING DAN BAHAN ORGANIK DALAM RUMEN SECARA IN VITRO Iswanto, Dimas Oqi Nur; Rimbawanto, Efka Aris; Bata, Muhamad
ANGON: Journal of Animal Science and Technology Vol 4 No 1 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1057.225 KB) | DOI: 10.20884/1.angon.2022.4.1.p1-6

Abstract

Background. Research "Supplementation of RDP (Rumen Degradable Protein) and UDP (Undegradable Protein) in Sheep Ration on In Vitro Degradation of Dry Matter and Organic Matter in Rumen". This study aims was determine the effect of RDP and UDP supplementation in sheep rations on dry matter degradation and in vitro organic matter degradation. Materials and methods The research material used were the rumen fluid of 3 sheep as a source of inoculum, treatment rations supplemented with RDP (urea-zeolite) and UDP (soybean meal protected by condensation tannins). The in vitro research method used a completely randomized design (CRD) with 5 treatments and 5 replications. The treatments tested were R1 = basal ration (30% elephant grass silage, 70% concentrate) + 1% RDP and 1% UDP; R2 = basal ration + RDP 1% and UDP 2% ; R3 = basal ration + RDP 1% and UDP 3% ; R4 = basal ration + RDP 2% and UDP 1% ; R5 = basal ration + RDP 3% and UDP 1%. The variables measured were dry matter degradation and organic matter degradation at 48 hours incubation. Results. The results showed that supplementation of RDP and UDP in sheep rations had no significant effect (P>0.05) on the value of dry matter and organic matter degradation. Conclusion. It can be concluded that the administration of RDP and UDP supplementation in sheep rations in vitro did not interfere with rumen microbial activity